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The map label for this gene is paaF [H]

Identifier: 158424490

GI number: 158424490

Start: 3264430

End: 3265188

Strand: Reverse

Name: paaF [H]

Synonym: AZC_2866

Alternate gene names: 158424490

Gene position: 3265188-3264430 (Counterclockwise)

Preceding gene: 158424491

Following gene: 158424489

Centisome position: 60.81

GC content: 70.88

Gene sequence:

>759_bases
ATGACCGAGAGCCTGCTCCTCAGCGATCCCCAGCCGGGCGTGCGCCTTCTCACCATCAATCGGCCGCAGCGGCGCAATGC
GCTGGACCGGGCGACCTATGGCGCGCTGGGTGAGGCCATCGCGGCGGCCGATGCGGATGAGGCGGTGCGCGCCATCGTGC
TCACCGGCGCGGAAGGCTGCTTCACCGCCGGCAATGATCTCTCCGATTTTCAGGACACCAGCGACACCGGGCCCAGCGCG
GGCCTGACCTTCCTTCAGGCGCTGACCGCCTGCGGCACGCCGCTGCTGGCGGCCGTCGAAGGTTTTGCGGTGGGCATCGG
CACGACGATGCTGCTCCATTGCGATCTCGCCTATGCGGGGGCGGGCGCCACCTTCCGCCTGCCGTTCGCCGCGCTCGGCC
TCTGCCCGGAAGGCGCCTCCAGCTACCTGCTGCCCCGCATCGCCGGCACCAAGATGGCGGCCGAGTTGCTGATGCTGGGC
GAGGCCTTCGGCCCGGATGTCGCCTGCGACGCGGGCCTCATCAATGCGGTGACGGCGAAGGACGGCGCCCTGGCACTGGC
GCTGGAGAAGGCCGGTGCGCTAGCGGCCCTGCCGCCGGAATCCGTGCTCGCCACCAAGCGTCTCCTGCGCGCCCATACGC
GCGATGCGGTGTCCGCCGCCCTTGCGCTGGAGGCGGAGAGCTTCCATGCCCTGCGGCGGGGGCCTGCGGCACAGGCCGCC
TTTGCCCGTTTCTTCAAGAAGCGGGACGGCGCATCCTGA

Upstream 100 bases:

>100_bases
CCCCGGTGGCGCAGGTGGTGGACCGTCTCGCGCACGAATATGCCACGGCGCGCGAGCGCCTCGCGGCCACCGCGGCCCGC
TTTGCCGGAGGAGAAGCCTG

Downstream 100 bases:

>100_bases
GCGCTCCTGATCCGCACCGCCGACCGGAAACCATGATGACCCAGCCCGAAGCCCAGACGAGCGACCTCGACGAAGGCGAT
GCCAAGGAAGACCGGCACTT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MTESLLLSDPQPGVRLLTINRPQRRNALDRATYGALGEAIAAADADEAVRAIVLTGAEGCFTAGNDLSDFQDTSDTGPSA
GLTFLQALTACGTPLLAAVEGFAVGIGTTMLLHCDLAYAGAGATFRLPFAALGLCPEGASSYLLPRIAGTKMAAELLMLG
EAFGPDVACDAGLINAVTAKDGALALALEKAGALAALPPESVLATKRLLRAHTRDAVSAALALEAESFHALRRGPAAQAA
FARFFKKRDGAS

Sequences:

>Translated_252_residues
MTESLLLSDPQPGVRLLTINRPQRRNALDRATYGALGEAIAAADADEAVRAIVLTGAEGCFTAGNDLSDFQDTSDTGPSA
GLTFLQALTACGTPLLAAVEGFAVGIGTTMLLHCDLAYAGAGATFRLPFAALGLCPEGASSYLLPRIAGTKMAAELLMLG
EAFGPDVACDAGLINAVTAKDGALALALEKAGALAALPPESVLATKRLLRAHTRDAVSAALALEAESFHALRRGPAAQAA
FARFFKKRDGAS
>Mature_251_residues
TESLLLSDPQPGVRLLTINRPQRRNALDRATYGALGEAIAAADADEAVRAIVLTGAEGCFTAGNDLSDFQDTSDTGPSAG
LTFLQALTACGTPLLAAVEGFAVGIGTTMLLHCDLAYAGAGATFRLPFAALGLCPEGASSYLLPRIAGTKMAAELLMLGE
AFGPDVACDAGLINAVTAKDGALALALEKAGALAALPPESVLATKRLLRAHTRDAVSAALALEAESFHALRRGPAAQAAF
ARFFKKRDGAS

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI260274832, Length=230, Percent_Identity=32.6086956521739, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI45643119, Length=230, Percent_Identity=32.6086956521739, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI260275230, Length=230, Percent_Identity=32.6086956521739, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI194097323, Length=212, Percent_Identity=30.6603773584906, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI221136756, Length=241, Percent_Identity=22.4066390041494, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI221136753, Length=241, Percent_Identity=22.4066390041494, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI221307494, Length=224, Percent_Identity=24.1071428571429, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI70995211, Length=232, Percent_Identity=28.8793103448276, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI116235487, Length=130, Percent_Identity=32.3076923076923, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI25453481, Length=219, Percent_Identity=25.1141552511416, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI4757966, Length=219, Percent_Identity=25.1141552511416, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI51538573, Length=219, Percent_Identity=25.1141552511416, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI51538581, Length=219, Percent_Identity=25.1141552511416, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI4757968, Length=165, Percent_Identity=27.8787878787879, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI49355787, Length=165, Percent_Identity=27.8787878787879, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI68989263, Length=183, Percent_Identity=30.6010928961749, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=33.8645418326693, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1787660, Length=254, Percent_Identity=29.5275590551181, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788597, Length=215, Percent_Identity=28.8372093023256, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI221142681, Length=239, Percent_Identity=29.7071129707113, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1790281, Length=183, Percent_Identity=28.9617486338798, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=242, Percent_Identity=30.1652892561983, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17549921, Length=180, Percent_Identity=37.7777777777778, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25145438, Length=220, Percent_Identity=30.9090909090909, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=223, Percent_Identity=29.5964125560538, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=213, Percent_Identity=28.6384976525822, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=234, Percent_Identity=25.2136752136752, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323314, Length=236, Percent_Identity=26.6949152542373, Blast_Score=75, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24654903, Length=211, Percent_Identity=32.2274881516588, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI20129971, Length=243, Percent_Identity=30.4526748971193, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653477, Length=243, Percent_Identity=30.4526748971193, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357171, Length=232, Percent_Identity=27.1551724137931, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19920382, Length=225, Percent_Identity=26.2222222222222, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653139, Length=204, Percent_Identity=27.9411764705882, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24650670, Length=236, Percent_Identity=29.2372881355932, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 25756; Mature: 25625

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESLLLSDPQPGVRLLTINRPQRRNALDRATYGALGEAIAAADADEAVRAIVLTGAEGC
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEECCCCC
FTAGNDLSDFQDTSDTGPSAGLTFLQALTACGTPLLAAVEGFAVGIGTTMLLHCDLAYAG
EECCCCCHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEECHHEEC
AGATFRLPFAALGLCPEGASSYLLPRIAGTKMAAELLMLGEAFGPDVACDAGLINAVTAK
CCCEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEECC
DGALALALEKAGALAALPPESVLATKRLLRAHTRDAVSAALALEAESFHALRRGPAAQAA
CCEEEEEEHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FARFFKKRDGAS
HHHHHHHHCCCC
>Mature Secondary Structure 
TESLLLSDPQPGVRLLTINRPQRRNALDRATYGALGEAIAAADADEAVRAIVLTGAEGC
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEECCCCC
FTAGNDLSDFQDTSDTGPSAGLTFLQALTACGTPLLAAVEGFAVGIGTTMLLHCDLAYAG
EECCCCCHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEECHHEEC
AGATFRLPFAALGLCPEGASSYLLPRIAGTKMAAELLMLGEAFGPDVACDAGLINAVTAK
CCCEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEECC
DGALALALEKAGALAALPPESVLATKRLLRAHTRDAVSAALALEAESFHALRRGPAAQAA
CCEEEEEEHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FARFFKKRDGAS
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]