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The map label for this gene is potB [H]

Identifier: 158424086

GI number: 158424086

Start: 2796775

End: 2797689

Strand: Reverse

Name: potB [H]

Synonym: AZC_2462

Alternate gene names: 158424086

Gene position: 2797689-2796775 (Counterclockwise)

Preceding gene: 158424087

Following gene: 158424085

Centisome position: 52.1

GC content: 66.67

Gene sequence:

>915_bases
ATGCCCGCCTCAGCGATGAACGGTCAGATGGAAATGGATGTGCCCGCGCCTCGCGCGCGGGCCGGCGTCCGGGTGTCGCC
GGCCTTCGGGCTGGCGACACCGGCCACGCTCTTCGTGCTGCTCGGGCTGGTGGCGCCGCTGGCGCTGATGTTCCGCTACA
GCCTCAACCGCTTCGTGCCTGGCCAGATGATGGTCGAGGCGCTGACCCTCGCCAATTATCAGAAGTTCTTTTCCGACAGC
TTCTATCAGGAGGTGCTGGGCACCACGCTCTGGGTGTCTGCCCTCTCCACCGTCCTGTGCCTGCTGGCGGGCTTCCCCGT
CGCTTACGCGCTGGTGCGCCACGCCGGGCCGAAGTGGAAGCCGCGCCTGCTCATCCTCATCATCCTGCCGCTGCTCATGG
GCAATGCGGTGCGCACCGCCGCCTGGATGGTGATCCTCGGCGACAAGGGCCTGTTCAATGCCGCGCTCGGCAGCGTGGGC
CTCTCCGCCGTCAAGCTGATGTACACGCCGACCGCCGTCGTCATCGGCCTCGTTTCCGTGATGCTGCCCTTCATGATCGT
AACGCTCCAGAGCGTGCTGGAGGGCATCGACGAAAATCTGGAGGCTGCCGCAGCCAGCCTCGGCGCGCCGCATGGGGTGG
TGCTGCGCCGAGTGGTGCTGCCGCTGGCGCTGCCGGGCCTCCTCGCCGGCACCATGCTGTGCTTCATCCTCTCGATGAAT
GCCTATGCGACGCCGGTGCTGATCGGCGGGCCGACCTTCCACATGATGGCGCCCACCGTCTATCAGCAGGTGGCCAAGGC
CATGAACTGGCCCTTCGGCGCCGCGCTCGCCTTCGTGCTGATGGCCGTCACCCTGTGCCTCACCACCACCGCAAATGTGC
TGGTGCAGCGGCATTACCGGAAGTGGAGCGAGTGA

Upstream 100 bases:

>100_bases
AGGCCGCGGCGCTCAAATTCCCCGATTATGAGCACGTGGCCAAGAATACGGCCGAATGGCTGGAGTGGTGGAACAAGAAC
ATCGCCCGCTGAGCCGCGAC

Downstream 100 bases:

>100_bases
TGGGCGCGCTTGCCATCCGCCGCATCTATGCCGGCCTCTCCGCCACCGTGCTCGCCTTCGTGCTCCTGCCGCTGGTCGCC
ATCGTCTGGGTGAGCTTCTT

Product: ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MPASAMNGQMEMDVPAPRARAGVRVSPAFGLATPATLFVLLGLVAPLALMFRYSLNRFVPGQMMVEALTLANYQKFFSDS
FYQEVLGTTLWVSALSTVLCLLAGFPVAYALVRHAGPKWKPRLLILIILPLLMGNAVRTAAWMVILGDKGLFNAALGSVG
LSAVKLMYTPTAVVIGLVSVMLPFMIVTLQSVLEGIDENLEAAAASLGAPHGVVLRRVVLPLALPGLLAGTMLCFILSMN
AYATPVLIGGPTFHMMAPTVYQQVAKAMNWPFGAALAFVLMAVTLCLTTTANVLVQRHYRKWSE

Sequences:

>Translated_304_residues
MPASAMNGQMEMDVPAPRARAGVRVSPAFGLATPATLFVLLGLVAPLALMFRYSLNRFVPGQMMVEALTLANYQKFFSDS
FYQEVLGTTLWVSALSTVLCLLAGFPVAYALVRHAGPKWKPRLLILIILPLLMGNAVRTAAWMVILGDKGLFNAALGSVG
LSAVKLMYTPTAVVIGLVSVMLPFMIVTLQSVLEGIDENLEAAAASLGAPHGVVLRRVVLPLALPGLLAGTMLCFILSMN
AYATPVLIGGPTFHMMAPTVYQQVAKAMNWPFGAALAFVLMAVTLCLTTTANVLVQRHYRKWSE
>Mature_303_residues
PASAMNGQMEMDVPAPRARAGVRVSPAFGLATPATLFVLLGLVAPLALMFRYSLNRFVPGQMMVEALTLANYQKFFSDSF
YQEVLGTTLWVSALSTVLCLLAGFPVAYALVRHAGPKWKPRLLILIILPLLMGNAVRTAAWMVILGDKGLFNAALGSVGL
SAVKLMYTPTAVVIGLVSVMLPFMIVTLQSVLEGIDENLEAAAASLGAPHGVVLRRVVLPLALPGLLAGTMLCFILSMNA
YATPVLIGGPTFHMMAPTVYQQVAKAMNWPFGAALAFVLMAVTLCLTTTANVLVQRHYRKWSE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=230, Percent_Identity=33.9130434782609, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1787369, Length=217, Percent_Identity=34.5622119815668, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787713, Length=240, Percent_Identity=30, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787368, Length=124, Percent_Identity=33.8709677419355, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32595; Mature: 32463

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASAMNGQMEMDVPAPRARAGVRVSPAFGLATPATLFVLLGLVAPLALMFRYSLNRFVP
CCCCCCCCCEEECCCCCHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
GQMMVEALTLANYQKFFSDSFYQEVLGTTLWVSALSTVLCLLAGFPVAYALVRHAGPKWK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PRLLILIILPLLMGNAVRTAAWMVILGDKGLFNAALGSVGLSAVKLMYTPTAVVIGLVSV
HHHHHHHHHHHHHCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLPFMIVTLQSVLEGIDENLEAAAASLGAPHGVVLRRVVLPLALPGLLAGTMLCFILSMN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AYATPVLIGGPTFHMMAPTVYQQVAKAMNWPFGAALAFVLMAVTLCLTTTANVLVQRHYR
CHHCCEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KWSE
HCCC
>Mature Secondary Structure 
PASAMNGQMEMDVPAPRARAGVRVSPAFGLATPATLFVLLGLVAPLALMFRYSLNRFVP
CCCCCCCCEEECCCCCHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC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HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]