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The map label for this gene is potD [H]

Identifier: 158424087

GI number: 158424087

Start: 2797698

End: 2798819

Strand: Reverse

Name: potD [H]

Synonym: AZC_2463

Alternate gene names: 158424087

Gene position: 2798819-2797698 (Counterclockwise)

Preceding gene: 158424088

Following gene: 158424086

Centisome position: 52.12

GC content: 62.75

Gene sequence:

>1122_bases
GTGCGCGCCTCTTCGCCACGCACGCATCATAAAAATCCAGAGGCGGACATGACGAAGACGACTACTCCCTTTTCTGCGCC
CCTTTCGCGCCGCTCTGTCCTCGTGGGCCTTGGCCTTGCTGCCGGCAGCGCCGTGTTCGGCCTGCCCCGTGGCGCCGCCG
CCCTTGAGGGCACACTTGTGGTCTCCAACTGGGGCGGCGACTGGAACGAACGCACCATCAAGTTCGTCGAGGCGCCGCTG
GTGGAAAGCCAGGGCATCAAGATCGTCCGCGACCTCGGCATGGAGCCGGAGCGCAAGGCCAAGCTCATCGCCGAGAAGCG
CCTGCGTCGCGGCACCATCGACGTCATCCATATCAATGAAGGCGACAGCGTCGAACTGAACGGCCAGGACGTGCTGGCCG
ACATCGATTTCGCCAAGGTGCCGAACTATGCGGACGTGGTGCCGGCGCTGAAGTCCAAGCCATTCTTCGTGCCGTGGCTC
TATAGCGGCGTGACGCTCATCTACAACAAGGACAAGGTGCCGAACCCGCCGAAGTCCTATGCGGAGTTGTGGGACAAGAA
ATGGGCCGGAAAGCTCGGCCTCACCAACCAGCTCTATTTCAATTATGTGATGATGGCCGGGCTCATCAAGGGCGGAAACG
TCACCAATACGGAAGAGGGCAAGAAGCGCCTCATCGAGCTGAAAGAACTCACCCAGCCGCGCATCTATGCGGCCCATCAG
CAACTGGCGGCAGGCCTTGCCAATGGCGAGGTGGACATCGCCATCAATTACAAGGCGCGCGGCCTCCAGTGGGCCAATGA
TGGGGCGCCGCTCGCCATCCAGTATCCGGCCGAGGGCGCTATCGCCGTCATGTTCGGCGCGGCCCTGCCCAAGCGGGCCC
CGAGCCCGGAGCTGGCCTATGTCTATTTCAACGCCATGCTCGATCCCAAGGCCATGGCGGGCCTCGCGGGCGCCAGCTTC
TACGCTCCGGCCAACGCCAAGGCAGCCCTTTCGCCCGAGCTGAAAGCGAAGATCGATTTCACGGCGGCGGAGGCCGCGGC
GCTCAAATTCCCCGATTATGAGCACGTGGCCAAGAATACGGCCGAATGGCTGGAGTGGTGGAACAAGAACATCGCCCGCT
GA

Upstream 100 bases:

>100_bases
TCGCCGATGTGGAGCCCCTGTCGCGCTGCATCGCCTATCTGGACGACGTGGGTACGCGCGCTGCCGCCTGAGGCCGGTCC
GTCCACGAGGCGCGCGCTCC

Downstream 100 bases:

>100_bases
GCCGCGACATGCCCGCCTCAGCGATGAACGGTCAGATGGAAATGGATGTGCCCGCGCCTCGCGCGCGGGCCGGCGTCCGG
GTGTCGCCGGCCTTCGGGCT

Product: twin-arginine translocation pathway signal precursor

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MRASSPRTHHKNPEADMTKTTTPFSAPLSRRSVLVGLGLAAGSAVFGLPRGAAALEGTLVVSNWGGDWNERTIKFVEAPL
VESQGIKIVRDLGMEPERKAKLIAEKRLRRGTIDVIHINEGDSVELNGQDVLADIDFAKVPNYADVVPALKSKPFFVPWL
YSGVTLIYNKDKVPNPPKSYAELWDKKWAGKLGLTNQLYFNYVMMAGLIKGGNVTNTEEGKKRLIELKELTQPRIYAAHQ
QLAAGLANGEVDIAINYKARGLQWANDGAPLAIQYPAEGAIAVMFGAALPKRAPSPELAYVYFNAMLDPKAMAGLAGASF
YAPANAKAALSPELKAKIDFTAAEAAALKFPDYEHVAKNTAEWLEWWNKNIAR

Sequences:

>Translated_373_residues
MRASSPRTHHKNPEADMTKTTTPFSAPLSRRSVLVGLGLAAGSAVFGLPRGAAALEGTLVVSNWGGDWNERTIKFVEAPL
VESQGIKIVRDLGMEPERKAKLIAEKRLRRGTIDVIHINEGDSVELNGQDVLADIDFAKVPNYADVVPALKSKPFFVPWL
YSGVTLIYNKDKVPNPPKSYAELWDKKWAGKLGLTNQLYFNYVMMAGLIKGGNVTNTEEGKKRLIELKELTQPRIYAAHQ
QLAAGLANGEVDIAINYKARGLQWANDGAPLAIQYPAEGAIAVMFGAALPKRAPSPELAYVYFNAMLDPKAMAGLAGASF
YAPANAKAALSPELKAKIDFTAAEAAALKFPDYEHVAKNTAEWLEWWNKNIAR
>Mature_373_residues
MRASSPRTHHKNPEADMTKTTTPFSAPLSRRSVLVGLGLAAGSAVFGLPRGAAALEGTLVVSNWGGDWNERTIKFVEAPL
VESQGIKIVRDLGMEPERKAKLIAEKRLRRGTIDVIHINEGDSVELNGQDVLADIDFAKVPNYADVVPALKSKPFFVPWL
YSGVTLIYNKDKVPNPPKSYAELWDKKWAGKLGLTNQLYFNYVMMAGLIKGGNVTNTEEGKKRLIELKELTQPRIYAAHQ
QLAAGLANGEVDIAINYKARGLQWANDGAPLAIQYPAEGAIAVMFGAALPKRAPSPELAYVYFNAMLDPKAMAGLAGASF
YAPANAKAALSPELKAKIDFTAAEAAALKFPDYEHVAKNTAEWLEWWNKNIAR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=280, Percent_Identity=25.7142857142857, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40702; Mature: 40702

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASSPRTHHKNPEADMTKTTTPFSAPLSRRSVLVGLGLAAGSAVFGLPRGAAALEGTLV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHCCCHHCCCCCCCCCEECEEE
VSNWGGDWNERTIKFVEAPLVESQGIKIVRDLGMEPERKAKLIAEKRLRRGTIDVIHINE
EECCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECC
GDSVELNGQDVLADIDFAKVPNYADVVPALKSKPFFVPWLYSGVTLIYNKDKVPNPPKSY
CCEEEECCCEEEEECCHHHCCCHHHHHHHHCCCCCEEEEHHCCEEEEEECCCCCCCCHHH
AELWDKKWAGKLGLTNQLYFNYVMMAGLIKGGNVTNTEEGKKRLIELKELTQPRIYAAHQ
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
QLAAGLANGEVDIAINYKARGLQWANDGAPLAIQYPAEGAIAVMFGAALPKRAPSPELAY
HHHHHCCCCCEEEEEEEEECCEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCEEE
VYFNAMLDPKAMAGLAGASFYAPANAKAALSPELKAKIDFTAAEAAALKFPDYEHVAKNT
EEEEECCCHHHHHHCCCCCEECCCCCCEECCCCCEEEEEEEHHHHHEECCCCHHHHHHHH
AEWLEWWNKNIAR
HHHHHHHHHHCCC
>Mature Secondary Structure
MRASSPRTHHKNPEADMTKTTTPFSAPLSRRSVLVGLGLAAGSAVFGLPRGAAALEGTLV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHCCCHHCCCCCCCCCEECEEE
VSNWGGDWNERTIKFVEAPLVESQGIKIVRDLGMEPERKAKLIAEKRLRRGTIDVIHINE
EECCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECC
GDSVELNGQDVLADIDFAKVPNYADVVPALKSKPFFVPWLYSGVTLIYNKDKVPNPPKSY
CCEEEECCCEEEEECCHHHCCCHHHHHHHHCCCCCEEEEHHCCEEEEEECCCCCCCCHHH
AELWDKKWAGKLGLTNQLYFNYVMMAGLIKGGNVTNTEEGKKRLIELKELTQPRIYAAHQ
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
QLAAGLANGEVDIAINYKARGLQWANDGAPLAIQYPAEGAIAVMFGAALPKRAPSPELAY
HHHHHCCCCCEEEEEEEEECCEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCEEE
VYFNAMLDPKAMAGLAGASFYAPANAKAALSPELKAKIDFTAAEAAALKFPDYEHVAKNT
EEEEECCCHHHHHHCCCCCEECCCCCCEECCCCCEEEEEEEHHHHHEECCCCHHHHHHHH
AEWLEWWNKNIAR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]