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The map label for this gene is nodW [H]

Identifier: 158423905

GI number: 158423905

Start: 2606427

End: 2607083

Strand: Direct

Name: nodW [H]

Synonym: AZC_2281

Alternate gene names: 158423905

Gene position: 2606427-2607083 (Clockwise)

Preceding gene: 158423904

Following gene: 158423906

Centisome position: 48.54

GC content: 66.21

Gene sequence:

>657_bases
GTGATGAAGGTCCAGAAATCCCGCAAGGAAACGGCGCCCGCGACACCCGTGGTGATCGTGGTGGACGACGACGCAGGTCT
GCGGGAGGCCCTGTCCAGCCTGTTCCGTTCGGTTGGCCTTGAAGTGGCGCTCTTCGCCTCGGCGCTGGAGATGCTCGCCG
CCCCCCTGCCCGACGCGCCACGCTGCCTCGTGCTCGACATCCGCCTGCCCGGCGTCAGCGGGCTCGACTTCCAGCAGCAG
TTGACGCGGCAGGACGTGGACATGCCCATCATCTTCATGACCGGACACGGGGATATTCCCATGTCGGTGCGGGCCATGAA
GGCGGGCGCCCTCGATTTCCTGACCAAGCCGTTCCGCGATCAGGACATGCTCGACGCGGTGCTGGCAGCCATCGCCATGG
ATCGCACGCGCCGCGACGAACGGGCCGCGTCCGACCACGTCCGCACGCTCTATGAGAGCCTGACCGAACGGGAGAAGCAG
ATCATGGGACTGGTCACCTCCGGCCTCATGAACAAGCAGGTGGCCGCGGAGGTGGGGCTCAGCGAGATCACGGTGAAGAT
CTATCGGGGCAACGTCATGCGGAAGATGGCCGCACGTTCCTTCGCCGACCTCGTGCGCATGGCGGAGGCGGTGGGCCTCA
GCAAGCCGAAGGCCTGA

Upstream 100 bases:

>100_bases
GCATCCTCGCAACCCCGGCCGAGCCGCGGGGGCTGCGCATGACCGTTCAACTCCCGATCACCGGCGACATGGCAGCGCCC
GGTCTGCAGGAGAAGGCCTC

Downstream 100 bases:

>100_bases
GCCGCCAAACGTGTGTATGATACTCCTGAGGCCCTTCGAGGGCCTATGGTGAGCCTCGATGCGGTGCGCATGCCGAACGG
CAGCGCGCCCCAAGAGGTGC

Product: nodulation protein W

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MMKVQKSRKETAPATPVVIVVDDDAGLREALSSLFRSVGLEVALFASALEMLAAPLPDAPRCLVLDIRLPGVSGLDFQQQ
LTRQDVDMPIIFMTGHGDIPMSVRAMKAGALDFLTKPFRDQDMLDAVLAAIAMDRTRRDERAASDHVRTLYESLTEREKQ
IMGLVTSGLMNKQVAAEVGLSEITVKIYRGNVMRKMAARSFADLVRMAEAVGLSKPKA

Sequences:

>Translated_218_residues
MMKVQKSRKETAPATPVVIVVDDDAGLREALSSLFRSVGLEVALFASALEMLAAPLPDAPRCLVLDIRLPGVSGLDFQQQ
LTRQDVDMPIIFMTGHGDIPMSVRAMKAGALDFLTKPFRDQDMLDAVLAAIAMDRTRRDERAASDHVRTLYESLTEREKQ
IMGLVTSGLMNKQVAAEVGLSEITVKIYRGNVMRKMAARSFADLVRMAEAVGLSKPKA
>Mature_218_residues
MMKVQKSRKETAPATPVVIVVDDDAGLREALSSLFRSVGLEVALFASALEMLAAPLPDAPRCLVLDIRLPGVSGLDFQQQ
LTRQDVDMPIIFMTGHGDIPMSVRAMKAGALDFLTKPFRDQDMLDAVLAAIAMDRTRRDERAASDHVRTLYESLTEREKQ
IMGLVTSGLMNKQVAAEVGLSEITVKIYRGNVMRKMAARSFADLVRMAEAVGLSKPKA

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=102, Percent_Identity=38.2352941176471, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788222, Length=208, Percent_Identity=25.9615384615385, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1790102, Length=194, Percent_Identity=29.8969072164948, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786599, Length=119, Percent_Identity=31.9327731092437, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788550, Length=142, Percent_Identity=26.7605633802817, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1786747, Length=160, Percent_Identity=24.375, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23875; Mature: 23875

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
6.4 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKVQKSRKETAPATPVVIVVDDDAGLREALSSLFRSVGLEVALFASALEMLAAPLPDAP
CCCCCHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCC
RCLVLDIRLPGVSGLDFQQQLTRQDVDMPIIFMTGHGDIPMSVRAMKAGALDFLTKPFRD
CEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
QDMLDAVLAAIAMDRTRRDERAASDHVRTLYESLTEREKQIMGLVTSGLMNKQVAAEVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
SEITVKIYRGNVMRKMAARSFADLVRMAEAVGLSKPKA
HHHEEEEHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMKVQKSRKETAPATPVVIVVDDDAGLREALSSLFRSVGLEVALFASALEMLAAPLPDAP
CCCCCHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCC
RCLVLDIRLPGVSGLDFQQQLTRQDVDMPIIFMTGHGDIPMSVRAMKAGALDFLTKPFRD
CEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
QDMLDAVLAAIAMDRTRRDERAASDHVRTLYESLTEREKQIMGLVTSGLMNKQVAAEVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
SEITVKIYRGNVMRKMAARSFADLVRMAEAVGLSKPKA
HHHEEEEHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]