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The map label for this gene is fixJ [H]

Identifier: 158423906

GI number: 158423906

Start: 2607188

End: 2607571

Strand: Direct

Name: fixJ [H]

Synonym: AZC_2282

Alternate gene names: 158423906

Gene position: 2607188-2607571 (Clockwise)

Preceding gene: 158423905

Following gene: 158423907

Centisome position: 48.55

GC content: 65.36

Gene sequence:

>384_bases
GTGTCTGTTTCTCCGATGATCACGATCATCGACGACGACGAGTTCGTCCGTCTCGCGACCGAGAGTTTCATGCGGTCGCT
GGGTTTCGAGACCCGGACCTTTGCCTGCGCAGAGGACTTCTTCTCGTCGCCGCTCCGACATGCCACGGACTGCATCATCA
CCGATGTGCAGATGCCGGGCATGAGCGGCGTGGAGCTTCTCGCCCGTCTGCGCGCTGAAGGAAGCGCCGTGCCGGTGATC
GTCGTGACGGCCTTTCCCAGCGACCGCATCCGCTCCCGCGCCACGGAACTCGGGGCGCTGGGTTTCTTTCCGAAGCCCTT
CAGCGGCGCCGACATTGTGGCCTGCATCGAGAAGGCCGTCGGGCCCGGACCGGCCGGCCCCTGA

Upstream 100 bases:

>100_bases
CCAAACGTGTGTATGATACTCCTGAGGCCCTTCGAGGGCCTATGGTGAGCCTCGATGCGGTGCGCATGCCGAACGGCAGC
GCGCCCCAAGAGGTGCTGAC

Downstream 100 bases:

>100_bases
GCGTCTCGTTTCAGCTCGACAGGACGGTTGCGCCGGCGGCCAGCACGATCAATCCAAGGACGATGAAGCCCCAGGTTCGC
CAACTCGGTGGTGCGCTGGC

Product: response regulator receiver

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MSVSPMITIIDDDEFVRLATESFMRSLGFETRTFACAEDFFSSPLRHATDCIITDVQMPGMSGVELLARLRAEGSAVPVI
VVTAFPSDRIRSRATELGALGFFPKPFSGADIVACIEKAVGPGPAGP

Sequences:

>Translated_127_residues
MSVSPMITIIDDDEFVRLATESFMRSLGFETRTFACAEDFFSSPLRHATDCIITDVQMPGMSGVELLARLRAEGSAVPVI
VVTAFPSDRIRSRATELGALGFFPKPFSGADIVACIEKAVGPGPAGP
>Mature_126_residues
SVSPMITIIDDDEFVRLATESFMRSLGFETRTFACAEDFFSSPLRHATDCIITDVQMPGMSGVELLARLRAEGSAVPVIV
VTAFPSDRIRSRATELGALGFFPKPFSGADIVACIEKAVGPGPAGP

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=116, Percent_Identity=31.0344827586207, Blast_Score=70, Evalue=5e-14,
Organism=Escherichia coli, GI1790299, Length=115, Percent_Identity=28.695652173913, Blast_Score=69, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13555; Mature: 13424

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSPMITIIDDDEFVRLATESFMRSLGFETRTFACAEDFFSSPLRHATDCIITDVQMPG
CCCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHEEECCCCCC
MSGVELLARLRAEGSAVPVIVVTAFPSDRIRSRATELGALGFFPKPFSGADIVACIEKAV
CHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHC
GPGPAGP
CCCCCCC
>Mature Secondary Structure 
SVSPMITIIDDDEFVRLATESFMRSLGFETRTFACAEDFFSSPLRHATDCIITDVQMPG
CCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHEEECCCCCC
MSGVELLARLRAEGSAVPVIVVTAFPSDRIRSRATELGALGFFPKPFSGADIVACIEKAV
CHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHC
GPGPAGP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]