Click here to switch to the map view.

The map label for this gene is cobQ

Identifier: 158423882

GI number: 158423882

Start: 2580953

End: 2582410

Strand: Direct

Name: cobQ

Synonym: AZC_2258

Alternate gene names: 158423882

Gene position: 2580953-2582410 (Clockwise)

Preceding gene: 158423881

Following gene: 158423884

Centisome position: 48.06

GC content: 69.41

Gene sequence:

>1458_bases
ATGGCGCGCGCGCTGATGTTTCAGGGCACCGGCTCGGACGTGGGCAAGTCCCTGCTGGTCGCCGGCCTCGCCCGCGCCTT
CGCTGACCGGGGCCTGAAGGTCCGCCCCTTCAAGCCGCAGAACATGTCCAACAATGCCGCCGTCACGGCCGACGGCGGCG
AGATTGGGCGGGCGCAGGCGCTTCAGGCCCGAGCTGCCCGCGTGCCTCTCTCCGTGCACATGAACCCCGTGCTGCTGAAG
CCGCAGAGCGAGGTTGGTGCGCAGGTGGTGGTGCAGGGCAGGGTGGTCGGCAGCGCCAAGGCGGCCGCCTATCAGCAGAT
GAAGGCAGGCCTCCTGCCCTCGGTTCTGGAGAGTTTTCGCCGGCTAAAGACCGAGGCCGACCTTGTGCTGGTGGAAGGTG
CCGGCTCCGCCTCCGAGGTCAATCTCAGGGCCAATGACATCGCCAACATGGGCTTTGCCCGTGCGGCGGACGTACCTGTG
GTACTGATCGGAGACATCGACCGAGGCGGCGTGATCGCGAGCCTCGTGGGCACGAAGACGGTGCTCGATCCGGCGGATGC
GGCGATGATCCAGGGCTTCATCGTCAACCGTTTCAGGGGCGATCCCGCCCTGTTCGGGAGCGGCATGGACCTCATCGCCG
GGCACACGGGCTGGGCCGCTCTCGGCCTCGTGCCCTTTTACACGGGCGCCGCCCGCCTGCCCGCCGAGGACGCGCTGGGC
GTCGCCGGCCCGCAGGCGCCGAAGCCCGGCGCGAAAGTGCGGATCGCGGTGCCGATCCTGCCCCATGTGGCGAATTTCGA
CGATCTCGATCCGCTGGACGCCGAGCCGGGTCTCGAGGTGCGGCGCATCCGTCCATCCGACGTCCTGCCGACGGATACGG
ATCTCGTGCTCCTCATCGGCTCCAAGGCCACCATCGCGGATCTCGCGGCCCTACGGGCCGGCGGCCTGCATCATGACATC
CAGGCCTTTGCACGTCGCGGCGGGCGGGTGCTGGGCCTGTGCGGCGGCTATCAGATGATGGGCGAGCATCTGTCCGATCC
GGATGGCGTGGAAGGCCCGCCCGGCTCGACGGAAGGCCTCGGGCTGCTGAAGGTGGGTACGGTGATGACCGGCGAGAAAC
GGCTGGTCGCCGTCACCGGTACCTCGCGGCTCGGTGCGCCTTTCTCCGGCTATGAAATGCACATCGGCGCAACGGAAGGC
GCCGACACCGCCCGCCCCTTCGCCGAGCTCGACGGCGCGGGCGCGGAAGGCGCCGTCTCGGCCGATGGCCGCATCATGGG
CACCTATGTGCACGGCCTGTTCGGGGATGACCGCCAGCGCGCGGCCCTCATGGGGCTGCTGGGCGCAGGGCCGGCTCAGG
TGGCCTATGAGGCAGGCGTCGAAGAGGCGCTGGACGGTCTTGCCGCTCACCTCTCGGCGCATCTCGACCTCGACCGCCTC
CTCAGCCTCGCGCGATGA

Upstream 100 bases:

>100_bases
TCGTCACCGAGATGCAGTTGGTGAAGCACCATTTCGCGGCCGGCGTGAAGGCGCAGGTCGGCATCGAGTTCTGAGCGCGT
CGACAGGAGCGGGGAGGGCC

Downstream 100 bases:

>100_bases
CGAGGCCCGCGAGCGCGATCAGCGTGACGAACACCACCAGCAGCGCGTCCGCCGCCACATAGAGGCGCAGGGCCGCGCGG
ATGTCGCGGGCGTCGATGTC

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MARALMFQGTGSDVGKSLLVAGLARAFADRGLKVRPFKPQNMSNNAAVTADGGEIGRAQALQARAARVPLSVHMNPVLLK
PQSEVGAQVVVQGRVVGSAKAAAYQQMKAGLLPSVLESFRRLKTEADLVLVEGAGSASEVNLRANDIANMGFARAADVPV
VLIGDIDRGGVIASLVGTKTVLDPADAAMIQGFIVNRFRGDPALFGSGMDLIAGHTGWAALGLVPFYTGAARLPAEDALG
VAGPQAPKPGAKVRIAVPILPHVANFDDLDPLDAEPGLEVRRIRPSDVLPTDTDLVLLIGSKATIADLAALRAGGLHHDI
QAFARRGGRVLGLCGGYQMMGEHLSDPDGVEGPPGSTEGLGLLKVGTVMTGEKRLVAVTGTSRLGAPFSGYEMHIGATEG
ADTARPFAELDGAGAEGAVSADGRIMGTYVHGLFGDDRQRAALMGLLGAGPAQVAYEAGVEEALDGLAAHLSAHLDLDRL
LSLAR

Sequences:

>Translated_485_residues
MARALMFQGTGSDVGKSLLVAGLARAFADRGLKVRPFKPQNMSNNAAVTADGGEIGRAQALQARAARVPLSVHMNPVLLK
PQSEVGAQVVVQGRVVGSAKAAAYQQMKAGLLPSVLESFRRLKTEADLVLVEGAGSASEVNLRANDIANMGFARAADVPV
VLIGDIDRGGVIASLVGTKTVLDPADAAMIQGFIVNRFRGDPALFGSGMDLIAGHTGWAALGLVPFYTGAARLPAEDALG
VAGPQAPKPGAKVRIAVPILPHVANFDDLDPLDAEPGLEVRRIRPSDVLPTDTDLVLLIGSKATIADLAALRAGGLHHDI
QAFARRGGRVLGLCGGYQMMGEHLSDPDGVEGPPGSTEGLGLLKVGTVMTGEKRLVAVTGTSRLGAPFSGYEMHIGATEG
ADTARPFAELDGAGAEGAVSADGRIMGTYVHGLFGDDRQRAALMGLLGAGPAQVAYEAGVEEALDGLAAHLSAHLDLDRL
LSLAR
>Mature_484_residues
ARALMFQGTGSDVGKSLLVAGLARAFADRGLKVRPFKPQNMSNNAAVTADGGEIGRAQALQARAARVPLSVHMNPVLLKP
QSEVGAQVVVQGRVVGSAKAAAYQQMKAGLLPSVLESFRRLKTEADLVLVEGAGSASEVNLRANDIANMGFARAADVPVV
LIGDIDRGGVIASLVGTKTVLDPADAAMIQGFIVNRFRGDPALFGSGMDLIAGHTGWAALGLVPFYTGAARLPAEDALGV
AGPQAPKPGAKVRIAVPILPHVANFDDLDPLDAEPGLEVRRIRPSDVLPTDTDLVLLIGSKATIADLAALRAGGLHHDIQ
AFARRGGRVLGLCGGYQMMGEHLSDPDGVEGPPGSTEGLGLLKVGTVMTGEKRLVAVTGTSRLGAPFSGYEMHIGATEGA
DTARPFAELDGAGAEGAVSADGRIMGTYVHGLFGDDRQRAALMGLLGAGPAQVAYEAGVEEALDGLAAHLSAHLDLDRLL
SLAR

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_AZOC5 (A8I5C0)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525174.1
- ProteinModelPortal:   A8I5C0
- GeneID:   5692199
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_2258
- HOGENOM:   HBG302271
- OMA:   AHTDLNP
- ProtClustDB:   PRK00784
- BioCyc:   ACAU438753:AZC_2258-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 49943; Mature: 49812

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 334-334 ACT_SITE 431-431

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARALMFQGTGSDVGKSLLVAGLARAFADRGLKVRPFKPQNMSNNAAVTADGGEIGRAQA
CCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCCCCHHHH
LQARAARVPLSVHMNPVLLKPQSEVGAQVVVQGRVVGSAKAAAYQQMKAGLLPSVLESFR
HHHHHHCCCEEECCCEEEECCCHHCCCEEEEEEEEECCHHHHHHHHHHHCCCHHHHHHHH
RLKTEADLVLVEGAGSASEVNLRANDIANMGFARAADVPVVLIGDIDRGGVIASLVGTKT
HHCCCCCEEEEECCCCCCEEEEEHHHHHHCCCCHHCCCCEEEEECCCCCCEEEEHHCCCH
VLDPADAAMIQGFIVNRFRGDPALFGSGMDLIAGHTGWAALGLVPFYTGAARLPAEDALG
HCCCHHHHHHHHHHHHHHCCCHHEECCCCHHEECCCCHHHHHHHHHHCCCCCCCCHHCCC
VAGPQAPKPGAKVRIAVPILPHVANFDDLDPLDAEPGLEVRRIRPSDVLPTDTDLVLLIG
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEC
SKATIADLAALRAGGLHHDIQAFARRGGRVLGLCGGYQMMGEHLSDPDGVEGPPGSTEGL
CCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCCCE
GLLKVGTVMTGEKRLVAVTGTSRLGAPFSGYEMHIGATEGADTARPFAELDGAGAEGAVS
EEEEECEEEECCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCCCCCEEC
ADGRIMGTYVHGLFGDDRQRAALMGLLGAGPAQVAYEAGVEEALDGLAAHLSAHLDLDRL
CCCEEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHCCCHHHH
LSLAR
HHHCC
>Mature Secondary Structure 
ARALMFQGTGSDVGKSLLVAGLARAFADRGLKVRPFKPQNMSNNAAVTADGGEIGRAQA
CCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCCCCHHHH
LQARAARVPLSVHMNPVLLKPQSEVGAQVVVQGRVVGSAKAAAYQQMKAGLLPSVLESFR
HHHHHHCCCEEECCCEEEECCCHHCCCEEEEEEEEECCHHHHHHHHHHHCCCHHHHHHHH
RLKTEADLVLVEGAGSASEVNLRANDIANMGFARAADVPVVLIGDIDRGGVIASLVGTKT
HHCCCCCEEEEECCCCCCEEEEEHHHHHHCCCCHHCCCCEEEEECCCCCCEEEEHHCCCH
VLDPADAAMIQGFIVNRFRGDPALFGSGMDLIAGHTGWAALGLVPFYTGAARLPAEDALG
HCCCHHHHHHHHHHHHHHCCCHHEECCCCHHEECCCCHHHHHHHHHHCCCCCCCCHHCCC
VAGPQAPKPGAKVRIAVPILPHVANFDDLDPLDAEPGLEVRRIRPSDVLPTDTDLVLLIG
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEC
SKATIADLAALRAGGLHHDIQAFARRGGRVLGLCGGYQMMGEHLSDPDGVEGPPGSTEGL
CCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCCCE
GLLKVGTVMTGEKRLVAVTGTSRLGAPFSGYEMHIGATEGADTARPFAELDGAGAEGAVS
EEEEECEEEECCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCCCCCEEC
ADGRIMGTYVHGLFGDDRQRAALMGLLGAGPAQVAYEAGVEEALDGLAAHLSAHLDLDRL
CCCEEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHCCCHHHH
LSLAR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA