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The map label for this gene is rfaE

Identifier: 158423878

GI number: 158423878

Start: 2576871

End: 2578310

Strand: Direct

Name: rfaE

Synonym: AZC_2254

Alternate gene names: 158423878

Gene position: 2576871-2578310 (Clockwise)

Preceding gene: 158423877

Following gene: 158423880

Centisome position: 47.99

GC content: 71.32

Gene sequence:

>1440_bases
ATGCTCGCCGAAACCCAGCTCGCGCCCATCGCCGTCATCGGCGACGTGATGGTGGACCGCTACATCACCGGCTCCGTCTC
GCGCATCTCTCCGGAAGCGCCGGTGCCGGTGCTCGTCCATGGCGCCGAACGCATCGTGCCCGGTGGCGCGGCCAACGTCG
CCGCCAATGCCGCCGCCCTCGGGGCGCCTGTGCTCCTCGTGGGTCTTGTCGGAGAGGACGAGGCGGCGAGCCAATTGGCC
GATGCGCTCGCCACCTATCCGGGCATCAGCCTCGCGCACATGGTGGTGGACGCCAAGCGGCCCACCATCACCAAGACGCG
GGTGATGAGCGGGCGCCAGCAGATCGTGCGCATCGACGCGGAGGTGACGCGGGCCTGCGACGCTGCCGAGGAGGCGGCGC
TGATTGGCGCGGCGGAAGCGGCCATCGCCAAGGCGGGTGTCGTGGTGCTCTCCGATTATGCCAAGGGCGTGCTGAGCGAT
GCGGTGATCGCCGCCGCCATGGCTGCGGCCAAGGCGCGCAATGTGCCGGTGATCGTCGATCCCAAGCGCCGCACCTTCGA
GGCCTATCGCGGCGCCACTCTTGTGACGCCCAATCGCCGGGAACTGGCCGAGGCCACCGGCCTGCCGGACGAGACGGATG
CGGACGCCGCCCGCGCGGCGGAGGCGGCCGGTCGCCAGTTCGGCGGCGATGTACTGGTGACGCGGGCCGAGAAGGGCATG
ACGCTCTGGCGGCAGGATGGGCGCGTGCTCCATGTGCCGGCCGAGGCGCGCGAGGTGTTCGACGTCTCGGGCGCGGGCGA
TACGGCATTGGCCGCTCTCGCCGTTTCGCTCGCCGGCGGGCAGAGCCTGGAGGCGAGCGTCGGCATCGCCAATGCGGCCG
CCGCGCTGGCCGTCGCGAAGCTCGGAACCGCCGTCGTGACGCGCGCGGAACTCAGGGCCGCGCTGGAGCGCACGGCGGCG
CAGATCGCACCGCCCGGTGCGCTCGTCTCCCGCGAGGATGCCTGCGCGGTGGTCGCCGCCTGGAAGGCGCAGGGGCTGCG
GGTGGTCTTCACCAACGGCTGCTTCGATCTCGTCCACCCCGGCCATGTGAGCCTGCTGGAACAGTCGGCCGCGCAGGGGG
ACCGTCTGGTGGTGGCGCTGAACACGGATGCCTCCGTCCGCCGCCTCAAGGGGCCGAGCCGCCCGCTTCAGGACGAGCAG
GCGCGGGCGCGGGTGATGGGCGCCATGCGCTGCGTCGATCTCGTCGTGCTGTTCGACGAGGAAACGCCGCTCGAAACCAT
CAAGGCGCTGCTGCCGGACGTGCTGGTGAAGGGCGCCGATTACGCCCCGCACGAGGTGGTTGGGGCCGACGTGGTGACGG
CAAACGGCGGCGAACTGGTGCTTGTGGATCTGGTGGCCGGCAAGTCCACCTCCAGCCTCGTTGCCAAGGCGCGGACCTGA

Upstream 100 bases:

>100_bases
AGCCGGCTACGCCAAGCCCTTCACCAGCCTTGAGGATGGCGTCGCCGATTATGTGACGCGCCATCTGCGTGGCGCCAACG
CGGCCTGAGCGGGGAAGAAA

Downstream 100 bases:

>100_bases
AAAATCCGCCTCAATTTATTGATCTGAAAAGAGAACGCCCCGAACCATCCGGCTCGGGGCGTCTTCATGTGATGCGTCAG
AGCAAGAGGCTCAGGCGGCG

Product: bifunctional protein

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MLAETQLAPIAVIGDVMVDRYITGSVSRISPEAPVPVLVHGAERIVPGGAANVAANAAALGAPVLLVGLVGEDEAASQLA
DALATYPGISLAHMVVDAKRPTITKTRVMSGRQQIVRIDAEVTRACDAAEEAALIGAAEAAIAKAGVVVLSDYAKGVLSD
AVIAAAMAAAKARNVPVIVDPKRRTFEAYRGATLVTPNRRELAEATGLPDETDADAARAAEAAGRQFGGDVLVTRAEKGM
TLWRQDGRVLHVPAEAREVFDVSGAGDTALAALAVSLAGGQSLEASVGIANAAAALAVAKLGTAVVTRAELRAALERTAA
QIAPPGALVSREDACAVVAAWKAQGLRVVFTNGCFDLVHPGHVSLLEQSAAQGDRLVVALNTDASVRRLKGPSRPLQDEQ
ARARVMGAMRCVDLVVLFDEETPLETIKALLPDVLVKGADYAPHEVVGADVVTANGGELVLVDLVAGKSTSSLVAKART

Sequences:

>Translated_479_residues
MLAETQLAPIAVIGDVMVDRYITGSVSRISPEAPVPVLVHGAERIVPGGAANVAANAAALGAPVLLVGLVGEDEAASQLA
DALATYPGISLAHMVVDAKRPTITKTRVMSGRQQIVRIDAEVTRACDAAEEAALIGAAEAAIAKAGVVVLSDYAKGVLSD
AVIAAAMAAAKARNVPVIVDPKRRTFEAYRGATLVTPNRRELAEATGLPDETDADAARAAEAAGRQFGGDVLVTRAEKGM
TLWRQDGRVLHVPAEAREVFDVSGAGDTALAALAVSLAGGQSLEASVGIANAAAALAVAKLGTAVVTRAELRAALERTAA
QIAPPGALVSREDACAVVAAWKAQGLRVVFTNGCFDLVHPGHVSLLEQSAAQGDRLVVALNTDASVRRLKGPSRPLQDEQ
ARARVMGAMRCVDLVVLFDEETPLETIKALLPDVLVKGADYAPHEVVGADVVTANGGELVLVDLVAGKSTSSLVAKART
>Mature_479_residues
MLAETQLAPIAVIGDVMVDRYITGSVSRISPEAPVPVLVHGAERIVPGGAANVAANAAALGAPVLLVGLVGEDEAASQLA
DALATYPGISLAHMVVDAKRPTITKTRVMSGRQQIVRIDAEVTRACDAAEEAALIGAAEAAIAKAGVVVLSDYAKGVLSD
AVIAAAMAAAKARNVPVIVDPKRRTFEAYRGATLVTPNRRELAEATGLPDETDADAARAAEAAGRQFGGDVLVTRAEKGM
TLWRQDGRVLHVPAEAREVFDVSGAGDTALAALAVSLAGGQSLEASVGIANAAAALAVAKLGTAVVTRAELRAALERTAA
QIAPPGALVSREDACAVVAAWKAQGLRVVFTNGCFDLVHPGHVSLLEQSAAQGDRLVVALNTDASVRRLKGPSRPLQDEQ
ARARVMGAMRCVDLVVLFDEETPLETIKALLPDVLVKGADYAPHEVVGADVVTANGGELVLVDLVAGKSTSSLVAKART

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Escherichia coli, GI1789432, Length=475, Percent_Identity=44.2105263157895, Blast_Score=353, Evalue=2e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_AZOC5 (A8I5B0)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001525170.1
- ProteinModelPortal:   A8I5B0
- SMR:   A8I5B0
- GeneID:   5691460
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_2254
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- ProtClustDB:   CLSK936243
- BioCyc:   ACAU438753:AZC_2254-MONOMER
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.- [C]

Molecular weight: Translated: 49174; Mature: 49174

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 267-267

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAETQLAPIAVIGDVMVDRYITGSVSRISPEAPVPVLVHGAERIVPGGAANVAANAAAL
CCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEECCHHCCCCCCCHHHHHHHHH
GAPVLLVGLVGEDEAASQLADALATYPGISLAHMVVDAKRPTITKTRVMSGRQQIVRIDA
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEHHEEECCCCCCHHHHHHCCCHHEEEECH
EVTRACDAAEEAALIGAAEAAIAKAGVVVLSDYAKGVLSDAVIAAAMAAAKARNVPVIVD
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
PKRRTFEAYRGATLVTPNRRELAEATGLPDETDADAARAAEAAGRQFGGDVLVTRAEKGM
CCHHHHHHHCCCEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
TLWRQDGRVLHVPAEAREVFDVSGAGDTALAALAVSLAGGQSLEASVGIANAAAALAVAK
EEEECCCCEEECCHHHHHHEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LGTAVVTRAELRAALERTAAQIAPPGALVSREDACAVVAAWKAQGLRVVFTNGCFDLVHP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCEECCCC
GHVSLLEQSAAQGDRLVVALNTDASVRRLKGPSRPLQDEQARARVMGAMRCVDLVVLFDE
CHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEEEEECC
ETPLETIKALLPDVLVKGADYAPHEVVGADVVTANGGELVLVDLVAGKSTSSLVAKART
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCHHHHHHCCCC
>Mature Secondary Structure
MLAETQLAPIAVIGDVMVDRYITGSVSRISPEAPVPVLVHGAERIVPGGAANVAANAAAL
CCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEECCHHCCCCCCCHHHHHHHHH
GAPVLLVGLVGEDEAASQLADALATYPGISLAHMVVDAKRPTITKTRVMSGRQQIVRIDA
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEHHEEECCCCCCHHHHHHCCCHHEEEECH
EVTRACDAAEEAALIGAAEAAIAKAGVVVLSDYAKGVLSDAVIAAAMAAAKARNVPVIVD
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
PKRRTFEAYRGATLVTPNRRELAEATGLPDETDADAARAAEAAGRQFGGDVLVTRAEKGM
CCHHHHHHHCCCEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
TLWRQDGRVLHVPAEAREVFDVSGAGDTALAALAVSLAGGQSLEASVGIANAAAALAVAK
EEEECCCCEEECCHHHHHHEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LGTAVVTRAELRAALERTAAQIAPPGALVSREDACAVVAAWKAQGLRVVFTNGCFDLVHP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCEECCCC
GHVSLLEQSAAQGDRLVVALNTDASVRRLKGPSRPLQDEQARARVMGAMRCVDLVVLFDE
CHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEEEEECC
ETPLETIKALLPDVLVKGADYAPHEVVGADVVTANGGELVLVDLVAGKSTSSLVAKART
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA