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The map label for this gene is artJ [H]

Identifier: 158423696

GI number: 158423696

Start: 2384990

End: 2385760

Strand: Reverse

Name: artJ [H]

Synonym: AZC_2072

Alternate gene names: 158423696

Gene position: 2385760-2384990 (Counterclockwise)

Preceding gene: 158423698

Following gene: 158423695

Centisome position: 44.43

GC content: 64.07

Gene sequence:

>771_bases
ATGCCAGAGCGCGGAACAGGAGACAGCATGTTTCTGAAATCTCTCGCCGCTCTGGCGGTCGGCATCGCAGTGGGCTTCTC
CGCACCCGTGATGGCGCAGGAGAGCTTCAAGGTGGGCGTCACCACCACCGGCGTTCCCTTCACCTTCGTGGACACCACCA
CCCAGCAGCCCACCGGCGCCATGGTGGACCTCGCCAAGGCGATCGCGACCGAAGTCGGCCTGAAGCCCACCTTCGAGCTG
ACCGCCTTCTCGGCGCTGGTGCCCTCGCTCAGCACCCACAAGATCGACGTCATCTCCGCCGGCATGCTTGCCACCGACGA
GCGCCGCAAGGTCGTGGATTTCAGCGAACCGGTCTATACCTATGGCGACGCCATGTTCGTGGCCGCCGACGCCAAGGATG
TCACGCTCGACGACCTCAAGGGCCAGGTGGTGGGCGCGCAGGTGGGCACCACCTTCGCCGACAGCCTAAAGGCCCTGAAC
ATCTTCAGCGAAGTGAAGCTCTACGAGAGCATCGCCGACATCATGCGCGACGTGAAGCTCGGCCGCATCAAGGCGGGCTT
TGGCGATCAGCCGATCATCGCCTACCAGATCGCCAAGAACCCCGGCCTCGGCGTGCGGCTCGTGAAAGGCTACAAGCCTC
TCAAGGAAGGCCAGGTCGCCCTCGCCATCGCCAAGGACAATCCGGCCCTGCTGACCAAGGTCAATGCCGCCATCCTCAAG
CTCAAGGCGAGCGGCGAGCTGGACAAGATCTTCGCCGCCTACGGCCTCTGA

Upstream 100 bases:

>100_bases
GCGGTTCCGATGCGGACCGGCCAAGGCAGCCCTTTCCTGCTGCCGATCCTTCGAGGCGGAACCAACACGCGCCGGACGGC
GAGCGCTTGCGCGCGCCCGG

Downstream 100 bases:

>100_bases
TCGACAACGCGGGCGGCGCGCCGCGCCGTCCGTTCCCCGGCCCATCGCGCCGGCAAGTACAGGTGCCGCGACGGCCCGCC
GTCGCATCCGTTGGCCCGCC

Product: amino acid ABC transporter

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MPERGTGDSMFLKSLAALAVGIAVGFSAPVMAQESFKVGVTTTGVPFTFVDTTTQQPTGAMVDLAKAIATEVGLKPTFEL
TAFSALVPSLSTHKIDVISAGMLATDERRKVVDFSEPVYTYGDAMFVAADAKDVTLDDLKGQVVGAQVGTTFADSLKALN
IFSEVKLYESIADIMRDVKLGRIKAGFGDQPIIAYQIAKNPGLGVRLVKGYKPLKEGQVALAIAKDNPALLTKVNAAILK
LKASGELDKIFAAYGL

Sequences:

>Translated_256_residues
MPERGTGDSMFLKSLAALAVGIAVGFSAPVMAQESFKVGVTTTGVPFTFVDTTTQQPTGAMVDLAKAIATEVGLKPTFEL
TAFSALVPSLSTHKIDVISAGMLATDERRKVVDFSEPVYTYGDAMFVAADAKDVTLDDLKGQVVGAQVGTTFADSLKALN
IFSEVKLYESIADIMRDVKLGRIKAGFGDQPIIAYQIAKNPGLGVRLVKGYKPLKEGQVALAIAKDNPALLTKVNAAILK
LKASGELDKIFAAYGL
>Mature_255_residues
PERGTGDSMFLKSLAALAVGIAVGFSAPVMAQESFKVGVTTTGVPFTFVDTTTQQPTGAMVDLAKAIATEVGLKPTFELT
AFSALVPSLSTHKIDVISAGMLATDERRKVVDFSEPVYTYGDAMFVAADAKDVTLDDLKGQVVGAQVGTTFADSLKALNI
FSEVKLYESIADIMRDVKLGRIKAGFGDQPIIAYQIAKNPGLGVRLVKGYKPLKEGQVALAIAKDNPALLTKVNAAILKL
KASGELDKIFAAYGL

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=239, Percent_Identity=35.5648535564854, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787088, Length=242, Percent_Identity=30.9917355371901, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787031, Length=242, Percent_Identity=33.0578512396694, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788228, Length=257, Percent_Identity=28.0155642023346, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1788649, Length=255, Percent_Identity=28.2352941176471, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27009; Mature: 26877

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPERGTGDSMFLKSLAALAVGIAVGFSAPVMAQESFKVGVTTTGVPFTFVDTTTQQPTGA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEEECCCCEEEEECCCCCCCCH
MVDLAKAIATEVGLKPTFELTAFSALVPSLSTHKIDVISAGMLATDERRKVVDFSEPVYT
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEECCCCCCEECCCCCCEE
YGDAMFVAADAKDVTLDDLKGQVVGAQVGTTFADSLKALNIFSEVKLYESIADIMRDVKL
CCCEEEEEECCCCCCHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRIKAGFGDQPIIAYQIAKNPGLGVRLVKGYKPLKEGQVALAIAKDNPALLTKVNAAILK
CEEECCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCEEEEEECCCCCEEEEECEEEEE
LKASGELDKIFAAYGL
EECCCCHHHHHHHHCC
>Mature Secondary Structure 
PERGTGDSMFLKSLAALAVGIAVGFSAPVMAQESFKVGVTTTGVPFTFVDTTTQQPTGA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEEECCCCEEEEECCCCCCCCH
MVDLAKAIATEVGLKPTFELTAFSALVPSLSTHKIDVISAGMLATDERRKVVDFSEPVYT
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEECCCCCCEECCCCCCEE
YGDAMFVAADAKDVTLDDLKGQVVGAQVGTTFADSLKALNIFSEVKLYESIADIMRDVKL
CCCEEEEEECCCCCCHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRIKAGFGDQPIIAYQIAKNPGLGVRLVKGYKPLKEGQVALAIAKDNPALLTKVNAAILK
CEEECCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCEEEEEECCCCCEEEEECEEEEE
LKASGELDKIFAAYGL
EECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]