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The map label for this gene is ubiF [H]

Identifier: 158423622

GI number: 158423622

Start: 2303566

End: 2304870

Strand: Reverse

Name: ubiF [H]

Synonym: AZC_1998

Alternate gene names: 158423622

Gene position: 2304870-2303566 (Counterclockwise)

Preceding gene: 158423633

Following gene: 158423619

Centisome position: 42.92

GC content: 70.73

Gene sequence:

>1305_bases
GTGCACAGGCGGCGTGCTGAGACGATGCGCCCGCAAGACGCCCTCGCGCGTTGCCGATATGATCCGCGCAATTTCCGGAG
GCGTACCGCCTCCATCGTTTGTCCGAAGGAGGCGTTCATGTCGCAGCCCGTCAGCAATGCACCGGGGATCGAAGCCGAAG
TGGCGGTGGTCGGTGCCGGGCCGGCCGGCCTCGCGACTGCCATCGGCTTTGCGGCATCGGGCCGGACCACCGTGCTCATC
ACCGGTCCGGAGCGCGGCATCGACCACCGCACGACCGCCCTGCTCGGCACCTCGATCCCGATCCTCGAGCGTTTCGGCGT
CTGGGACCGCATCGCCCCTCACACCGCGCCGCTGCGGGACATGCGCATCGTGGACGGCACGCGCCGCCTCGTCCGCGCGC
CGGAAGTGACCTTCAAGGCCAGCGAGATCGGCCTCGAAGCCTTCGGCTACAATATCGAGAACGAGCCCCTGCGCCGGGAG
TTGGGGGCAGCCGCCGAGGAGACGAACGGCCTCAGTCTCGCCCGCGCATCCCTCACCGATCTGCGCACGGACGCGGACGG
CGTGACGCTCAGCCTCGATGACGGTCGCTCGGTGCGCGCCACGCTCGTGGTGGCGGCGGACGGGCGCAACTCCCGCGTCC
GCGAGGCGTGCGGCATCGGCGTGAAGCGGCGCGAATATCCGCAGGTGGCGGTCACCGCCACGGTACGCCACACCCGTCCC
CACTGGGACATCTCCACCGAATTCCACACCGAAACCGGCCCCTTTACCCTGGTGCCCCTGCCCGGAGACCGCTCCAGCAT
CGTCTGCGTGGTGGATGACCGCGAGGCGGTGCGCCTCCTCGGACTCGACCTGCCGGCGCTGGCGCGGGAATTCGAGCGGC
GGGCCCATTCCATCCTGGGTCCTTTCGAGGTCGAGGAAGGCCGAGCGGCCTTCCCGCTGGTGGCCGAAACGGCCCGCGAG
ATCACCGCGCCCCGCGTCGCCCTGATGGCGGAAGCGGCCCATGTGATGCCGCCCATCGGAGCGCAGGGCCTGAACCTTGG
GCTCCGGGACGCGGTTGCGCTGGTGGATCTCGCGGCCGAGCCCGGCCTCGACCTCGGCGGAGCGGAAATGCTGAAGCGCT
ATGCGGGCGCCCGCGACCGCGACATCCACGGCCGCATGACGGCGGTGGATATGCTCAACCGCTCGCTCCTCTCCGACCTC
GTGCCGGTGCAGAGCGTGCGCGGCTTCGGCCTTTATCTGCTCGACCGCGTGGGGCCGCTGCGGCGCACGGTGATGCGCCT
CGGCCTCGGCAACCGCGCGGCCTGA

Upstream 100 bases:

>100_bases
GATGAATTCGGATCGCCCCGACCATTTCCGCCTCCCGCGCTTGAAACGCCCGCGATCATAGGGGCGTTGAAGTTCCGGGC
AAGGCGCCTGCCAAAGCCCT

Downstream 100 bases:

>100_bases
GCGTCCCTTATTTCACGACCGAGATGCGGGGCGACGTCACCGCCGCCTCGCGCGGCTTGGCGGGAGCATCCCGCATGCCG
AAATCGGTGCGCGCCACGAG

Product: ubiquinone biosynthesis hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MHRRRAETMRPQDALARCRYDPRNFRRRTASIVCPKEAFMSQPVSNAPGIEAEVAVVGAGPAGLATAIGFAASGRTTVLI
TGPERGIDHRTTALLGTSIPILERFGVWDRIAPHTAPLRDMRIVDGTRRLVRAPEVTFKASEIGLEAFGYNIENEPLRRE
LGAAAEETNGLSLARASLTDLRTDADGVTLSLDDGRSVRATLVVAADGRNSRVREACGIGVKRREYPQVAVTATVRHTRP
HWDISTEFHTETGPFTLVPLPGDRSSIVCVVDDREAVRLLGLDLPALAREFERRAHSILGPFEVEEGRAAFPLVAETARE
ITAPRVALMAEAAHVMPPIGAQGLNLGLRDAVALVDLAAEPGLDLGGAEMLKRYAGARDRDIHGRMTAVDMLNRSLLSDL
VPVQSVRGFGLYLLDRVGPLRRTVMRLGLGNRAA

Sequences:

>Translated_434_residues
MHRRRAETMRPQDALARCRYDPRNFRRRTASIVCPKEAFMSQPVSNAPGIEAEVAVVGAGPAGLATAIGFAASGRTTVLI
TGPERGIDHRTTALLGTSIPILERFGVWDRIAPHTAPLRDMRIVDGTRRLVRAPEVTFKASEIGLEAFGYNIENEPLRRE
LGAAAEETNGLSLARASLTDLRTDADGVTLSLDDGRSVRATLVVAADGRNSRVREACGIGVKRREYPQVAVTATVRHTRP
HWDISTEFHTETGPFTLVPLPGDRSSIVCVVDDREAVRLLGLDLPALAREFERRAHSILGPFEVEEGRAAFPLVAETARE
ITAPRVALMAEAAHVMPPIGAQGLNLGLRDAVALVDLAAEPGLDLGGAEMLKRYAGARDRDIHGRMTAVDMLNRSLLSDL
VPVQSVRGFGLYLLDRVGPLRRTVMRLGLGNRAA
>Mature_434_residues
MHRRRAETMRPQDALARCRYDPRNFRRRTASIVCPKEAFMSQPVSNAPGIEAEVAVVGAGPAGLATAIGFAASGRTTVLI
TGPERGIDHRTTALLGTSIPILERFGVWDRIAPHTAPLRDMRIVDGTRRLVRAPEVTFKASEIGLEAFGYNIENEPLRRE
LGAAAEETNGLSLARASLTDLRTDADGVTLSLDDGRSVRATLVVAADGRNSRVREACGIGVKRREYPQVAVTATVRHTRP
HWDISTEFHTETGPFTLVPLPGDRSSIVCVVDDREAVRLLGLDLPALAREFERRAHSILGPFEVEEGRAAFPLVAETARE
ITAPRVALMAEAAHVMPPIGAQGLNLGLRDAVALVDLAAEPGLDLGGAEMLKRYAGARDRDIHGRMTAVDMLNRSLLSDL
VPVQSVRGFGLYLLDRVGPLRRTVMRLGLGNRAA

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=422, Percent_Identity=26.303317535545, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI32967307, Length=329, Percent_Identity=27.9635258358663, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1786883, Length=399, Percent_Identity=30.5764411027569, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789273, Length=393, Percent_Identity=29.5165394402036, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789274, Length=393, Percent_Identity=29.5165394402036, Blast_Score=123, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25146631, Length=441, Percent_Identity=26.3038548752834, Blast_Score=114, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6321694, Length=464, Percent_Identity=23.2758620689655, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 47051; Mature: 47051

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRRRAETMRPQDALARCRYDPRNFRRRTASIVCPKEAFMSQPVSNAPGIEAEVAVVGAG
CCCCHHHHCCCHHHHHHCCCCHHHHHHHHHEEECCHHHHHCCCCCCCCCCCEEEEEEECC
PAGLATAIGFAASGRTTVLITGPERGIDHRTTALLGTSIPILERFGVWDRIAPHTAPLRD
CHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCHHHHHCCCHHHCCCCCCCCHH
MRIVDGTRRLVRAPEVTFKASEIGLEAFGYNIENEPLRRELGAAAEETNGLSLARASLTD
EEEHHHHHHHHCCCCCEEEHHHHCHHHHCCCCCCCHHHHHHCCCHHCCCCCHHHHHHHHH
LRTDADGVTLSLDDGRSVRATLVVAADGRNSRVREACGIGVKRREYPQVAVTATVRHTRP
HCCCCCCEEEEECCCCCEEEEEEEEECCCCCHHHHHHCCCCCCCCCCCEEEEEEEECCCC
HWDISTEFHTETGPFTLVPLPGDRSSIVCVVDDREAVRLLGLDLPALAREFERRAHSILG
CCCCCCEEECCCCCEEEEECCCCCCEEEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHCC
PFEVEEGRAAFPLVAETAREITAPRVALMAEAAHVMPPIGAQGLNLGLRDAVALVDLAAE
CEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCC
PGLDLGGAEMLKRYAGARDRDIHGRMTAVDMLNRSLLSDLVPVQSVRGFGLYLLDRVGPL
CCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHH
RRTVMRLGLGNRAA
HHHHHHHCCCCCCC
>Mature Secondary Structure
MHRRRAETMRPQDALARCRYDPRNFRRRTASIVCPKEAFMSQPVSNAPGIEAEVAVVGAG
CCCCHHHHCCCHHHHHHCCCCHHHHHHHHHEEECCHHHHHCCCCCCCCCCCEEEEEEECC
PAGLATAIGFAASGRTTVLITGPERGIDHRTTALLGTSIPILERFGVWDRIAPHTAPLRD
CHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCHHHHHCCCHHHCCCCCCCCHH
MRIVDGTRRLVRAPEVTFKASEIGLEAFGYNIENEPLRRELGAAAEETNGLSLARASLTD
EEEHHHHHHHHCCCCCEEEHHHHCHHHHCCCCCCCHHHHHHCCCHHCCCCCHHHHHHHHH
LRTDADGVTLSLDDGRSVRATLVVAADGRNSRVREACGIGVKRREYPQVAVTATVRHTRP
HCCCCCCEEEEECCCCCEEEEEEEEECCCCCHHHHHHCCCCCCCCCCCEEEEEEEECCCC
HWDISTEFHTETGPFTLVPLPGDRSSIVCVVDDREAVRLLGLDLPALAREFERRAHSILG
CCCCCCEEECCCCCEEEEECCCCCCEEEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHCC
PFEVEEGRAAFPLVAETAREITAPRVALMAEAAHVMPPIGAQGLNLGLRDAVALVDLAAE
CEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCC
PGLDLGGAEMLKRYAGARDRDIHGRMTAVDMLNRSLLSDLVPVQSVRGFGLYLLDRVGPL
CCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHH
RRTVMRLGLGNRAA
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]