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The map label for this gene is fdoH [H]

Identifier: 158422782

GI number: 158422782

Start: 1291133

End: 1292110

Strand: Reverse

Name: fdoH [H]

Synonym: AZC_1158

Alternate gene names: 158422782

Gene position: 1292110-1291133 (Counterclockwise)

Preceding gene: 158422783

Following gene: 158422781

Centisome position: 24.06

GC content: 63.91

Gene sequence:

>978_bases
GTGAGGGAGGGTCGAACCATGTTGCAACCAGCACCGAACCCTCCGGTCGCCCCCACCCCGGCGAAGTTCTCGGACCAGGA
TCTGCTGCGGAAGTCGGCGTCCACCATCACCCCGCCGGCCCAGCGCCAGACGGAGGTGGCCAAGCTCATCGACGTGTCGA
AGTGCATCGGCTGCAAGGCCTGCCAGGCGGCATGTCTTGAGTGGAACAACCTCCACGAGGATATCGGCACCTTCAACGGG
TCCTACACCAACCCGCCGGACCTGACCGCCAACAGCTTCACGCTGATGCGGTTCACCGAGTGGGTGAACCCGGAGACCGA
CAACCTTGAGTGGCTGATCCGCAAGGACGGCTGCATGCATTGCGAGGACCCGGGCTGCCTCAAGGCCTGCCCGGCCCCCG
GCGCCATCGTGCAGTACACCAACGGCATCGTGGATTTCGTCCACGATAACTGCATCGGCTGCGGCTATTGCGTGAAGGGC
TGTCCCTTCAACATTCCCCGCATCTCCAAGGTCGACAACAAGGCGTACAAGTGCACGCTGTGTTCCGACCGCGTGGCGGT
GGGCCAGTGGCCCGCCTGCGCCAAGGCGTGCCCCACCCAGGCCATCGCCTTCGGCTCCAAGGAGGACATGAAGAAGCATG
CCGCCGAGCGCGTCACGGATCTGAAGTCCCGCGGCTACAAGAACGCGGGCCTCTATGATCCGCCGGGCGTCTCCGGCACG
CACGTCATGTACGTGCTCCAGCATGCTGACAAGCCGAGCATCTATGCCGGCCTTCCGGACAATCCGCGCATCTCCCCGAT
CGTGGAAGCCTGGAAGGGCGTCACCAAGTATGTCGGCATGGGCGTCATGGGCTTCGCCGCCATCGCAGGCGTCTTCCATT
ACCTCGTCGCGGGCGCCAACAAGGTGAGCCCGGAAGATGAGGAACAGGCCCAGAAGCTGGCAGAAGGCGTCAAGGGTACG
AAAGGGGACAAGGCATGA

Upstream 100 bases:

>100_bases
CCGCGAACGTGCTCACCCCGTTCGTCGGTGACGCGAACATCGAGACGCCGGAGTTCAAGGCGTTTCTGGTCAATATCGAG
CCCACGACCGGGCCGGTGGC

Downstream 100 bases:

>100_bases
GCGATAATCCCCGCCCCGCCCCCAGCGATGTCATGCCCGGCGACAGCGTGCAGCCGGGCCGTCCGGTGACTGTGAACCGA
TACACCGTCGGGGCGCGGAT

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MREGRTMLQPAPNPPVAPTPAKFSDQDLLRKSASTITPPAQRQTEVAKLIDVSKCIGCKACQAACLEWNNLHEDIGTFNG
SYTNPPDLTANSFTLMRFTEWVNPETDNLEWLIRKDGCMHCEDPGCLKACPAPGAIVQYTNGIVDFVHDNCIGCGYCVKG
CPFNIPRISKVDNKAYKCTLCSDRVAVGQWPACAKACPTQAIAFGSKEDMKKHAAERVTDLKSRGYKNAGLYDPPGVSGT
HVMYVLQHADKPSIYAGLPDNPRISPIVEAWKGVTKYVGMGVMGFAAIAGVFHYLVAGANKVSPEDEEQAQKLAEGVKGT
KGDKA

Sequences:

>Translated_325_residues
MREGRTMLQPAPNPPVAPTPAKFSDQDLLRKSASTITPPAQRQTEVAKLIDVSKCIGCKACQAACLEWNNLHEDIGTFNG
SYTNPPDLTANSFTLMRFTEWVNPETDNLEWLIRKDGCMHCEDPGCLKACPAPGAIVQYTNGIVDFVHDNCIGCGYCVKG
CPFNIPRISKVDNKAYKCTLCSDRVAVGQWPACAKACPTQAIAFGSKEDMKKHAAERVTDLKSRGYKNAGLYDPPGVSGT
HVMYVLQHADKPSIYAGLPDNPRISPIVEAWKGVTKYVGMGVMGFAAIAGVFHYLVAGANKVSPEDEEQAQKLAEGVKGT
KGDKA
>Mature_325_residues
MREGRTMLQPAPNPPVAPTPAKFSDQDLLRKSASTITPPAQRQTEVAKLIDVSKCIGCKACQAACLEWNNLHEDIGTFNG
SYTNPPDLTANSFTLMRFTEWVNPETDNLEWLIRKDGCMHCEDPGCLKACPAPGAIVQYTNGIVDFVHDNCIGCGYCVKG
CPFNIPRISKVDNKAYKCTLCSDRVAVGQWPACAKACPTQAIAFGSKEDMKKHAAERVTDLKSRGYKNAGLYDPPGVSGT
HVMYVLQHADKPSIYAGLPDNPRISPIVEAWKGVTKYVGMGVMGFAAIAGVFHYLVAGANKVSPEDEEQAQKLAEGVKGT
KGDKA

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=302, Percent_Identity=58.9403973509934, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1787749, Length=293, Percent_Identity=59.3856655290102, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1787122, Length=175, Percent_Identity=32.5714285714286, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI2367345, Length=180, Percent_Identity=30, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787872, Length=175, Percent_Identity=31.4285714285714, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789370, Length=182, Percent_Identity=30.2197802197802, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI226510944, Length=184, Percent_Identity=31.5217391304348, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082179, Length=175, Percent_Identity=26.8571428571429, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI2367245, Length=167, Percent_Identity=27.5449101796407, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788811, Length=176, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787963, Length=158, Percent_Identity=26.5822784810127, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789067, Length=185, Percent_Identity=29.1891891891892, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082180, Length=167, Percent_Identity=28.1437125748503, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 35170; Mature: 35170

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREGRTMLQPAPNPPVAPTPAKFSDQDLLRKSASTITPPAQRQTEVAKLIDVSKCIGCKA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHH
CQAACLEWNNLHEDIGTFNGSYTNPPDLTANSFTLMRFTEWVNPETDNLEWLIRKDGCMH
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCEEEEEECCCCCC
CEDPGCLKACPAPGAIVQYTNGIVDFVHDNCIGCGYCVKGCPFNIPRISKVDNKAYKCTL
CCCCCCCCCCCCCCHHHHHHCHHHHHHHCCCCCCCHHHCCCCCCCCCCHHCCCCCEEEEE
CSDRVAVGQWPACAKACPTQAIAFGSKEDMKKHAAERVTDLKSRGYKNAGLYDPPGVSGT
CCCCCCCCCCCHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
HVMYVLQHADKPSIYAGLPDNPRISPIVEAWKGVTKYVGMGVMGFAAIAGVFHYLVAGAN
EEEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KVSPEDEEQAQKLAEGVKGTKGDKA
CCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MREGRTMLQPAPNPPVAPTPAKFSDQDLLRKSASTITPPAQRQTEVAKLIDVSKCIGCKA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHH
CQAACLEWNNLHEDIGTFNGSYTNPPDLTANSFTLMRFTEWVNPETDNLEWLIRKDGCMH
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCEEEEEECCCCCC
CEDPGCLKACPAPGAIVQYTNGIVDFVHDNCIGCGYCVKGCPFNIPRISKVDNKAYKCTL
CCCCCCCCCCCCCCHHHHHHCHHHHHHHCCCCCCCHHHCCCCCCCCCCHHCCCCCEEEEE
CSDRVAVGQWPACAKACPTQAIAFGSKEDMKKHAAERVTDLKSRGYKNAGLYDPPGVSGT
CCCCCCCCCCCHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
HVMYVLQHADKPSIYAGLPDNPRISPIVEAWKGVTKYVGMGVMGFAAIAGVFHYLVAGAN
EEEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KVSPEDEEQAQKLAEGVKGTKGDKA
CCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]