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The map label for this gene is fdoI [H]

Identifier: 158422781

GI number: 158422781

Start: 1290429

End: 1291136

Strand: Reverse

Name: fdoI [H]

Synonym: AZC_1157

Alternate gene names: 158422781

Gene position: 1291136-1290429 (Counterclockwise)

Preceding gene: 158422782

Following gene: 158422780

Centisome position: 24.04

GC content: 64.41

Gene sequence:

>708_bases
ATGAGCGATAATCCCCGCCCCGCCCCCAGCGATGTCATGCCCGGCGACAGCGTGCAGCCGGGCCGTCCGGTGACTGTGAA
CCGATACACCGTCGGGGCGCGGATCAACCACTGGATCACCGCCATCAGCCTGATCCTGCTGGCGTTGTCCGGCCTGTCGC
TGTTCACGCCGAGCCTGTTCTTCCTGACCAACCTGTTCGGTGGCGGACAGCTCACCCGCGCCATCCATCCCTGGATCGGC
GTGGTGCTGTTCTTCTCCTTCTCCATCCTGTTCATCCGCTTCTGGCGGGCGAACCTGCCCAAGCGGGAAGACGTGACATG
GGTCGCGCGCAGCGGCGATGTCCTGATGGGTCACGAGGAACGTCTTCCTGAACTCGGCAAGTACAATGCCGGTCAAAAGG
TGGTGTTCTGGGGCATGTCGGCGCTGATCATCGTTCTGATCGTCAGCGGCCTGATCGTGTGGGAGCGATATTTCTTCACC
TACACGAGCATTCCCACGCAGCGTGTGGCGCTCATCGTCCACTCGCTGGCGGCGGTCGGCATCATCTGCATCTGGATCAT
CCACGTCTACGCGGCCATCTGGGTCAAGGGCACCATGAGCGCCATGACCAGCGGCAAGGTGACGGGCGGCTGGGCCTGGC
GGCATCACCGCAAGTGGCTGCGGGAGCTCGTGGCCGGCAAGCGCGCCGGCGGCACCCCGGCCGAGTGA

Upstream 100 bases:

>100_bases
TCCATTACCTCGTCGCGGGCGCCAACAAGGTGAGCCCGGAAGATGAGGAACAGGCCCAGAAGCTGGCAGAAGGCGTCAAG
GGTACGAAAGGGGACAAGGC

Downstream 100 bases:

>100_bases
GCCCCGCCTTCTGACGTCATCCGCCCGCGCGGCCCCCAGCCGCGCGGGCTTTCGTTCCTGCCGCGATCCGTCGCCCTCGC
GCAATGCGTGCAGAGGCCTA

Product: formate dehydrogenase subunit gamma

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MSDNPRPAPSDVMPGDSVQPGRPVTVNRYTVGARINHWITAISLILLALSGLSLFTPSLFFLTNLFGGGQLTRAIHPWIG
VVLFFSFSILFIRFWRANLPKREDVTWVARSGDVLMGHEERLPELGKYNAGQKVVFWGMSALIIVLIVSGLIVWERYFFT
YTSIPTQRVALIVHSLAAVGIICIWIIHVYAAIWVKGTMSAMTSGKVTGGWAWRHHRKWLRELVAGKRAGGTPAE

Sequences:

>Translated_235_residues
MSDNPRPAPSDVMPGDSVQPGRPVTVNRYTVGARINHWITAISLILLALSGLSLFTPSLFFLTNLFGGGQLTRAIHPWIG
VVLFFSFSILFIRFWRANLPKREDVTWVARSGDVLMGHEERLPELGKYNAGQKVVFWGMSALIIVLIVSGLIVWERYFFT
YTSIPTQRVALIVHSLAAVGIICIWIIHVYAAIWVKGTMSAMTSGKVTGGWAWRHHRKWLRELVAGKRAGGTPAE
>Mature_234_residues
SDNPRPAPSDVMPGDSVQPGRPVTVNRYTVGARINHWITAISLILLALSGLSLFTPSLFFLTNLFGGGQLTRAIHPWIGV
VLFFSFSILFIRFWRANLPKREDVTWVARSGDVLMGHEERLPELGKYNAGQKVVFWGMSALIIVLIVSGLIVWERYFFTY
TSIPTQRVALIVHSLAAVGIICIWIIHVYAAIWVKGTMSAMTSGKVTGGWAWRHHRKWLRELVAGKRAGGTPAE

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=202, Percent_Identity=46.5346534653465, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1787750, Length=190, Percent_Identity=41.0526315789474, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 26198; Mature: 26067

Theoretical pI: Translated: 10.96; Mature: 10.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNPRPAPSDVMPGDSVQPGRPVTVNRYTVGARINHWITAISLILLALSGLSLFTPSLF
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHH
FLTNLFGGGQLTRAIHPWIGVVLFFSFSILFIRFWRANLPKREDVTWVARSGDVLMGHEE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEECCHH
RLPELGKYNAGQKVVFWGMSALIIVLIVSGLIVWERYFFTYTSIPTQRVALIVHSLAAVG
HCCHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IICIWIIHVYAAIWVKGTMSAMTSGKVTGGWAWRHHRKWLRELVAGKRAGGTPAE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SDNPRPAPSDVMPGDSVQPGRPVTVNRYTVGARINHWITAISLILLALSGLSLFTPSLF
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHH
FLTNLFGGGQLTRAIHPWIGVVLFFSFSILFIRFWRANLPKREDVTWVARSGDVLMGHEE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEECCHH
RLPELGKYNAGQKVVFWGMSALIIVLIVSGLIVWERYFFTYTSIPTQRVALIVHSLAAVG
HCCHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IICIWIIHVYAAIWVKGTMSAMTSGKVTGGWAWRHHRKWLRELVAGKRAGGTPAE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]