Click here to switch to the map view.

The map label for this gene is yisK [H]

Identifier: 158422393

GI number: 158422393

Start: 869842

End: 870738

Strand: Reverse

Name: yisK [H]

Synonym: AZC_0769

Alternate gene names: 158422393

Gene position: 870738-869842 (Counterclockwise)

Preceding gene: 158422395

Following gene: 158422392

Centisome position: 16.22

GC content: 64.1

Gene sequence:

>897_bases
GTGACGCAGCAGACGATCCAGCGTTGTGCGATCGCAGAAGGGACAGACATGAAGCTCGCAACATTCAAAATCGGCGCCCG
GCCGACCTGGGGCGTCATCGCGGGAGATGAAGCGCTGGACGTCGGCGCCGTGCTTCGGGACCGCTATGGAAGCCTGAAGG
CCGTTTTGGCAGCCGATGCGCTGTCTCAGGTGGAAGCGGCCCTGCCGCAGGCCGCACGCCATCCGCTTTCGGAGATCACC
TGGCTGCCGGTCATCCCGGATTCCAACAAGGTGCTCTGCATCGGCCTCAATTATGAGATGCACCGGCTGGAGACAGGACG
GGCCGTGGTGGAACACCCCACGGTGTTCACCCGCTTCGCCGACAGCCAGATCGGCCATCTGGCGCACATCATCCGACCGC
GCGTGTCCCAGCACCTCGATTTCGAAGGTGAGCTTGCGATCATCATCGGCAAGCCCGGCCGCTACATCACGCGCGAGAAC
GCCTGGAGCCACATCGCCGGCTACAGCTGCTACAATGAAGGCAGCATCCGCGATTTCCAGCGCCACACCCACCAGTTCGC
ACCGGGCAAGAACTTCCCCGGCACCGGCGCCTTCGGCCCGTGGATGATGACGCCGGACGAGGCGGGCGACATCACCACCA
TGAAGCTGGAGACGCGGCTGAACGGGACGGTGGTGCAGGAAGCGCTCATCTCGCAGATGATCTTCGATATCCCCTGCCAG
ATCGAATATTGCTCCACCTTCACCAACCTCGAACCCGGCGACGTCATCGTGACCGGAACGCCCGGCGGCGTCGGCGCCAA
GCGCACGCCTCCGCTTTGGATGAAGCCCGGCGACATCGTCGAGGTGGAGATCGACCGCGTCGGCATCCTGCGCAACGGCG
TTGCCGACGAAGCCTGA

Upstream 100 bases:

>100_bases
TTTTTATGGCGCAGCGCGCCAGTTTCATACATAAAATCTGTCTGAGAGATTTTTTAGCGATATAGTCCTGTCCTGCCGTC
GTCGCGCCATCGAGCGGAGC

Downstream 100 bases:

>100_bases
CCGATGACGGTGGCGGATGGCAGGCCCCATCCGCAACCCCAAGCAGACCTGACGGGCGCGCGGCTCTCCAGCCGGACGTG
AAGGCCGGCGCGCGCCCGCT

Product: 2-keto-4-pentenoate hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTQQTIQRCAIAEGTDMKLATFKIGARPTWGVIAGDEALDVGAVLRDRYGSLKAVLAADALSQVEAALPQAARHPLSEIT
WLPVIPDSNKVLCIGLNYEMHRLETGRAVVEHPTVFTRFADSQIGHLAHIIRPRVSQHLDFEGELAIIIGKPGRYITREN
AWSHIAGYSCYNEGSIRDFQRHTHQFAPGKNFPGTGAFGPWMMTPDEAGDITTMKLETRLNGTVVQEALISQMIFDIPCQ
IEYCSTFTNLEPGDVIVTGTPGGVGAKRTPPLWMKPGDIVEVEIDRVGILRNGVADEA

Sequences:

>Translated_298_residues
MTQQTIQRCAIAEGTDMKLATFKIGARPTWGVIAGDEALDVGAVLRDRYGSLKAVLAADALSQVEAALPQAARHPLSEIT
WLPVIPDSNKVLCIGLNYEMHRLETGRAVVEHPTVFTRFADSQIGHLAHIIRPRVSQHLDFEGELAIIIGKPGRYITREN
AWSHIAGYSCYNEGSIRDFQRHTHQFAPGKNFPGTGAFGPWMMTPDEAGDITTMKLETRLNGTVVQEALISQMIFDIPCQ
IEYCSTFTNLEPGDVIVTGTPGGVGAKRTPPLWMKPGDIVEVEIDRVGILRNGVADEA
>Mature_297_residues
TQQTIQRCAIAEGTDMKLATFKIGARPTWGVIAGDEALDVGAVLRDRYGSLKAVLAADALSQVEAALPQAARHPLSEITW
LPVIPDSNKVLCIGLNYEMHRLETGRAVVEHPTVFTRFADSQIGHLAHIIRPRVSQHLDFEGELAIIIGKPGRYITRENA
WSHIAGYSCYNEGSIRDFQRHTHQFAPGKNFPGTGAFGPWMMTPDEAGDITTMKLETRLNGTVVQEALISQMIFDIPCQI
EYCSTFTNLEPGDVIVTGTPGGVGAKRTPPLWMKPGDIVEVEIDRVGILRNGVADEA

Specific function: Unknown

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=301, Percent_Identity=34.5514950166113, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI40786394, Length=300, Percent_Identity=34.3333333333333, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI215422413, Length=203, Percent_Identity=32.0197044334975, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI13654274, Length=203, Percent_Identity=32.0197044334975, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI66348062, Length=203, Percent_Identity=32.0197044334975, Blast_Score=95, Evalue=7e-20,
Organism=Escherichia coli, GI1787428, Length=201, Percent_Identity=30.3482587064677, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17557057, Length=198, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28572127, Length=250, Percent_Identity=34.4, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24663695, Length=221, Percent_Identity=29.4117647058824, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI28571789, Length=204, Percent_Identity=27.4509803921569, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: NA

Molecular weight: Translated: 32616; Mature: 32484

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQTIQRCAIAEGTDMKLATFKIGARPTWGVIAGDEALDVGAVLRDRYGSLKAVLAADA
CCHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHH
LSQVEAALPQAARHPLSEITWLPVIPDSNKVLCIGLNYEMHRLETGRAVVEHPTVFTRFA
HHHHHHHHHHHHHCCHHHCEEEEEECCCCCEEEEECCCEEEEECCCCEEEECCEEEEECC
DSQIGHLAHIIRPRVSQHLDFEGELAIIIGKPGRYITRENAWSHIAGYSCYNEGSIRDFQ
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCHHHHCCCEECCCCCHHHHH
RHTHQFAPGKNFPGTGAFGPWMMTPDEAGDITTMKLETRLNGTVVQEALISQMIFDIPCQ
HHHHHCCCCCCCCCCCCCCCCEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCE
IEYCSTFTNLEPGDVIVTGTPGGVGAKRTPPLWMKPGDIVEVEIDRVGILRNGVADEA
EEEHHHHCCCCCCCEEEECCCCCCCCCCCCCCEECCCCEEEEEECEEEEECCCCCCCC
>Mature Secondary Structure 
TQQTIQRCAIAEGTDMKLATFKIGARPTWGVIAGDEALDVGAVLRDRYGSLKAVLAADA
CHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHH
LSQVEAALPQAARHPLSEITWLPVIPDSNKVLCIGLNYEMHRLETGRAVVEHPTVFTRFA
HHHHHHHHHHHHHCCHHHCEEEEEECCCCCEEEEECCCEEEEECCCCEEEECCEEEEECC
DSQIGHLAHIIRPRVSQHLDFEGELAIIIGKPGRYITRENAWSHIAGYSCYNEGSIRDFQ
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCHHHHCCCEECCCCCHHHHH
RHTHQFAPGKNFPGTGAFGPWMMTPDEAGDITTMKLETRLNGTVVQEALISQMIFDIPCQ
HHHHHCCCCCCCCCCCCCCCCEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCE
IEYCSTFTNLEPGDVIVTGTPGGVGAKRTPPLWMKPGDIVEVEIDRVGILRNGVADEA
EEEHHHHCCCCCCCEEEECCCCCCCCCCCCCCEECCCCEEEEEECEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]