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The map label for this gene is nirA [H]

Identifier: 158422305

GI number: 158422305

Start: 752157

End: 753926

Strand: Reverse

Name: nirA [H]

Synonym: AZC_0681

Alternate gene names: 158422305

Gene position: 753926-752157 (Counterclockwise)

Preceding gene: 158422306

Following gene: 158422304

Centisome position: 14.04

GC content: 68.81

Gene sequence:

>1770_bases
ATGAGCGGCGACTTTACAGTGGATCAGAAACGCTATCTGGAAGGTTTCATGTCCGGCGCGCAGGTGGCCCGCGCGGGTCG
CGCGCCCGGCGGCGGCTCTCCCGCCACGGCCGAGCCGACCGGACCGGATGCGCCGAGCCTCAAGGCCATGGCGCGCACGG
AAGCGGAGGGCAAGAAGCTCTGCCCCGAGGAGAAGGCCAAGCGCGAGGAAATGGGCCTCGATGCCTGGCCCCGCATGGAA
GAAGCCGCGCGCGAGGGCGTCTACCCCAAGGGGCCGGACATCCTGCGCTGGAAGTATCACGGCCTGTTCTATGTCGCCCC
CGCGCAGGACAGCTTCATGTGCCGCCTCAGGATGCCCAACGGCATCCTCACCCACTGGCAGTTCCGGGGCATCGCCGACA
TCGCCGAAATCCACGGCGGCGGCTATGCGGACGTGACAACGCGCGCCAATCTCCAGATCCGCGAGATCCCGGCCACCGAC
GGCGTGATCCTGCTGGAGAAGCTGGTGGACCTCGGACTCACCGCCAAGGGCTCCGGCGGCGACAACATCCGCAACGTCAC
GGGCACACCCACCGCTGGCATCGACCCGCAGGAGCTGCTGGACACCCGCCCCTATGCCAAGGCGTGGCACCATCACATCC
TCAACACCCGCACGCTCTATGGCCTGCCGCGCAAGTTCAACGTGGCCTTCGACGGCGCCGGCCAGATCGGCGCGCTGGAG
GACACCAACGACATCGGCTTCCAGGCCGTGGAGGTGAAGGAGGGCTTCGGCGTCGAACCCGGCGTGTGGTTCCGCCTTGC
GCTCGGCGGCATCACCGGCCACAAGGATTTCGCCCGCGATACCGGCGTCATCCTGCCCCCTGCGGACGCCACCAAGGTGG
CCGATGCCATCATCCGCGTCTTCATCGACACCGGCGACCGCACCGACCGCAAGAAGGCCCGCCTCAAATACGTGCTCGAT
GCGCTGGGCTTCGACGGCTTCCTGAAGAAGGTGGAAGAGCGGCTGGGCGCCCCCTTCGTCCGCGTGCCGGCAGACGCCGT
GAAACCGCGCCCGGAGGCGGACCGCATGGCCCATCTCGGCGTGCGGCCGCAGAAGCAGGCGGGGCTGAACTGGATCGGCA
TCGGCGTCCCGGTGGGCCGCATGACGGCCGCCCAGATGCGCGGCCTTGCGGATATCGCCCAGAACCTCGGGGATGGCGAT
CTGCGCCTCACCGTCTGGCAGAACGTCCTCGTTTCCGGCGTGCCGGATGCACGGGTGACGGTGGCGAAGGCCGCCATCAC
CGCGCTCGGCCTCACGGCGGATGCGGATGCGCTTCAGGCCGGCATCGTCGCCTGCACGGGGGCGGCCGGCTGCCGCTTCG
GCAATGCGGACACTAAGGCCACCGCGCGCGCCATCGCCGCTTATTGCGGCCCGCGCGTGGCGCTGGACAGCCCGGTGAAC
ATCCACGTCACCGGCTGCCACAATTCCTGCGCCCAGCATTATATCGGCGATATCGGCCTCATCGGCGCGCGCGTGGCCAT
CAATGAGGATGGCGACACGGTGGATGGCTACAACCTGCTGGTGGGCGGCGGTTTCGGCACGGAGGCCGCCATCGCGCAGG
AGCTGTTCGAGCAGGTGAAGGCGGAAGACGCACCCGCCCTCGTGGAGCGCCTGCTCAGCGGCTGGACTGCGCAAAGGCTG
CCGGGCGAGACCTTCGCCGCCTTCACCCGCCGCCTTGGCCCCGCCGCCCTGAAATCCCTCGTCGAGACCGCCATGGAGCG
CGCCGAATGA

Upstream 100 bases:

>100_bases
TATTTCCTCGACCTCATCACGAACCGCACCCCCATCGCGCCGCTGCGCGCCGCGCTCGCCTTCGGGCGGCCGGCGCTGGC
GGCGTGAGGAGAGAGACATC

Downstream 100 bases:

>100_bases
CCCTCCAGACGCGAACCCCTCTGGTGCCCTTCCTGCCGGAAAGCGCCCCTTTCTCGGCCGAGCAGCGGGCCTGGCTCAAC
GGCTTCTTCGCCGGCATTTT

Product: ferredoxin-nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MSGDFTVDQKRYLEGFMSGAQVARAGRAPGGGSPATAEPTGPDAPSLKAMARTEAEGKKLCPEEKAKREEMGLDAWPRME
EAAREGVYPKGPDILRWKYHGLFYVAPAQDSFMCRLRMPNGILTHWQFRGIADIAEIHGGGYADVTTRANLQIREIPATD
GVILLEKLVDLGLTAKGSGGDNIRNVTGTPTAGIDPQELLDTRPYAKAWHHHILNTRTLYGLPRKFNVAFDGAGQIGALE
DTNDIGFQAVEVKEGFGVEPGVWFRLALGGITGHKDFARDTGVILPPADATKVADAIIRVFIDTGDRTDRKKARLKYVLD
ALGFDGFLKKVEERLGAPFVRVPADAVKPRPEADRMAHLGVRPQKQAGLNWIGIGVPVGRMTAAQMRGLADIAQNLGDGD
LRLTVWQNVLVSGVPDARVTVAKAAITALGLTADADALQAGIVACTGAAGCRFGNADTKATARAIAAYCGPRVALDSPVN
IHVTGCHNSCAQHYIGDIGLIGARVAINEDGDTVDGYNLLVGGGFGTEAAIAQELFEQVKAEDAPALVERLLSGWTAQRL
PGETFAAFTRRLGPAALKSLVETAMERAE

Sequences:

>Translated_589_residues
MSGDFTVDQKRYLEGFMSGAQVARAGRAPGGGSPATAEPTGPDAPSLKAMARTEAEGKKLCPEEKAKREEMGLDAWPRME
EAAREGVYPKGPDILRWKYHGLFYVAPAQDSFMCRLRMPNGILTHWQFRGIADIAEIHGGGYADVTTRANLQIREIPATD
GVILLEKLVDLGLTAKGSGGDNIRNVTGTPTAGIDPQELLDTRPYAKAWHHHILNTRTLYGLPRKFNVAFDGAGQIGALE
DTNDIGFQAVEVKEGFGVEPGVWFRLALGGITGHKDFARDTGVILPPADATKVADAIIRVFIDTGDRTDRKKARLKYVLD
ALGFDGFLKKVEERLGAPFVRVPADAVKPRPEADRMAHLGVRPQKQAGLNWIGIGVPVGRMTAAQMRGLADIAQNLGDGD
LRLTVWQNVLVSGVPDARVTVAKAAITALGLTADADALQAGIVACTGAAGCRFGNADTKATARAIAAYCGPRVALDSPVN
IHVTGCHNSCAQHYIGDIGLIGARVAINEDGDTVDGYNLLVGGGFGTEAAIAQELFEQVKAEDAPALVERLLSGWTAQRL
PGETFAAFTRRLGPAALKSLVETAMERAE
>Mature_588_residues
SGDFTVDQKRYLEGFMSGAQVARAGRAPGGGSPATAEPTGPDAPSLKAMARTEAEGKKLCPEEKAKREEMGLDAWPRMEE
AAREGVYPKGPDILRWKYHGLFYVAPAQDSFMCRLRMPNGILTHWQFRGIADIAEIHGGGYADVTTRANLQIREIPATDG
VILLEKLVDLGLTAKGSGGDNIRNVTGTPTAGIDPQELLDTRPYAKAWHHHILNTRTLYGLPRKFNVAFDGAGQIGALED
TNDIGFQAVEVKEGFGVEPGVWFRLALGGITGHKDFARDTGVILPPADATKVADAIIRVFIDTGDRTDRKKARLKYVLDA
LGFDGFLKKVEERLGAPFVRVPADAVKPRPEADRMAHLGVRPQKQAGLNWIGIGVPVGRMTAAQMRGLADIAQNLGDGDL
RLTVWQNVLVSGVPDARVTVAKAAITALGLTADADALQAGIVACTGAAGCRFGNADTKATARAIAAYCGPRVALDSPVNI
HVTGCHNSCAQHYIGDIGLIGARVAINEDGDTVDGYNLLVGGGFGTEAAIAQELFEQVKAEDAPALVERLLSGWTAQRLP
GETFAAFTRRLGPAALKSLVETAMERAE

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=539, Percent_Identity=25.7884972170686, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6322597, Length=544, Percent_Identity=25.1838235294118, Blast_Score=114, Evalue=6e-26,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 62801; Mature: 62670

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDFTVDQKRYLEGFMSGAQVARAGRAPGGGSPATAEPTGPDAPSLKAMARTEAEGKKL
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCC
CPEEKAKREEMGLDAWPRMEEAAREGVYPKGPDILRWKYHGLFYVAPAQDSFMCRLRMPN
CCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCEEEEECCCCCEEEEEECCC
GILTHWQFRGIADIAEIHGGGYADVTTRANLQIREIPATDGVILLEKLVDLGLTAKGSGG
CCEEEEECCCHHHHHHHCCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHCCCCCCCCCC
DNIRNVTGTPTAGIDPQELLDTRPYAKAWHHHILNTRTLYGLPRKFNVAFDGAGQIGALE
CCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCHHHCCCCCEEEEEECCCCCCCCCC
DTNDIGFQAVEVKEGFGVEPGVWFRLALGGITGHKDFARDTGVILPPADATKVADAIIRV
CCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCHHHHCCCCEECCCCHHHHHHHHHHH
FIDTGDRTDRKKARLKYVLDALGFDGFLKKVEERLGAPFVRVPADAVKPRPEADRMAHLG
HCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCEEECCHHHCCCCCCHHHHHHCC
VRPQKQAGLNWIGIGVPVGRMTAAQMRGLADIAQNLGDGDLRLTVWQNVLVSGVPDARVT
CCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCHHH
VAKAAITALGLTADADALQAGIVACTGAAGCRFGNADTKATARAIAAYCGPRVALDSPVN
HHHHHHHHHCCCCCHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCE
IHVTGCHNSCAQHYIGDIGLIGARVAINEDGDTVDGYNLLVGGGFGTEAAIAQELFEQVK
EEEECCCHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHC
AEDAPALVERLLSGWTAQRLPGETFAAFTRRLGPAALKSLVETAMERAE
CCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGDFTVDQKRYLEGFMSGAQVARAGRAPGGGSPATAEPTGPDAPSLKAMARTEAEGKKL
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCC
CPEEKAKREEMGLDAWPRMEEAAREGVYPKGPDILRWKYHGLFYVAPAQDSFMCRLRMPN
CCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCEEEEECCCCCEEEEEECCC
GILTHWQFRGIADIAEIHGGGYADVTTRANLQIREIPATDGVILLEKLVDLGLTAKGSGG
CCEEEEECCCHHHHHHHCCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHCCCCCCCCCC
DNIRNVTGTPTAGIDPQELLDTRPYAKAWHHHILNTRTLYGLPRKFNVAFDGAGQIGALE
CCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCHHHCCCCCEEEEEECCCCCCCCCC
DTNDIGFQAVEVKEGFGVEPGVWFRLALGGITGHKDFARDTGVILPPADATKVADAIIRV
CCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCHHHHCCCCEECCCCHHHHHHHHHHH
FIDTGDRTDRKKARLKYVLDALGFDGFLKKVEERLGAPFVRVPADAVKPRPEADRMAHLG
HCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCEEECCHHHCCCCCCHHHHHHCC
VRPQKQAGLNWIGIGVPVGRMTAAQMRGLADIAQNLGDGDLRLTVWQNVLVSGVPDARVT
CCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCHHH
VAKAAITALGLTADADALQAGIVACTGAAGCRFGNADTKATARAIAAYCGPRVALDSPVN
HHHHHHHHHCCCCCHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCE
IHVTGCHNSCAQHYIGDIGLIGARVAINEDGDTVDGYNLLVGGGFGTEAAIAQELFEQVK
EEEECCCHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHC
AEDAPALVERLLSGWTAQRLPGETFAAFTRRLGPAALKSLVETAMERAE
CCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]