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The map label for this gene is nasD [H]

Identifier: 158422306

GI number: 158422306

Start: 753940

End: 755142

Strand: Reverse

Name: nasD [H]

Synonym: AZC_0682

Alternate gene names: 158422306

Gene position: 755142-753940 (Counterclockwise)

Preceding gene: 158422307

Following gene: 158422305

Centisome position: 14.06

GC content: 70.91

Gene sequence:

>1203_bases
GTGAGCGAGCCTCTCGTCATCATCGGCAACGGCATGGCCGCCACCAAGCTCTGCGAAGAGCTCGGGGCCTGCGGGCTCGG
CCGCTATTCCGTCGCCGTCGTGGGAGCGGAGCCGGGCCTTGCCTATAATCGCGTGCTGCTCTCCGACGTGCTCGCCGGCA
AGCTGCCGGCCACCGATCTTGAAATGAAGCCCGCCAGTTGGTGGGCGAAGCAGGGCGTGACCCTGCTCTATGGAACGCCC
GCCGCTTCCATCGACCGGGAAAGCCGGGCCGTGGTGCTGGCGGACGGGCGTCGCCTGCCCTTCAGCCGGCTGGTGCTCGC
CACCGGCTCGCATCCCATCCGCCTGCCGCTGCCGGGCGCGGACCTGCCCGGCGTCACCACCTTCCGCGATCTGGCGGATG
TTTCGGCTCTGCTCGCGGCGGCAAAGCCCGGCGTGCGCGCCGTGGTGATCGGCGGCGGCCTTCTGGGGCTGGAAGCCGCC
ATCGGCCTTGCGGGCGCAGGCGTTGCGACCACCCTCGTCCATCTGATGGACCGGCTGATGGAGCGCCAGCTCGACGCCAC
TGCCGCCGATCTGCTGCGTCAGGCGGTGGAAGCGCGCGGCGTTACCGTGCGGCTTCAGGCCCACAGCGCCGCCATCGAAG
GCGAAAGCCATGTCACGGGACTGCGGCTCGCGGATGGCGAGGTGCTGCCCGCCGATCTGGTGGTGATGGCTTGCGGCGTG
CGCCCGAATGCCGATCTCGCCCGCGCAACGGGCCTCACCTGCAATCGCGGCGTGGTGGTGGACGATCATCTCGCCACCAG
CGATCCCGCCATCTTCGCGCTGGGCGAATGCGCCGAGCACCGGGGCATGGCCTATGGCCTCGTCGCCCCCGCCTATGAGC
AGGCGCACGTGCTCGCGCGGCATCTGGCGGGCAAGCCGGCGGCTTATCCGGGCAGCGTCGTCTCGACGAACCTCAAGGTG
TCCGGCGTGCCGGTCTTCTCGGCCGGCGACATCTCCGGCGAGGGGGCGCAGGACATCGTGCTGCGTGACCGCGCCCATGG
CATCTATCGCCGCCTCTTCATCCGCGACGGCGTGCTCGCTGGCGCCGTGCTCTTCGGCGACACCGCCGACGGGCTCTATT
TCCTCGACCTCATCACGAACCGCACCCCCATCGCGCCGCTGCGCGCCGCGCTCGCCTTCGGGCGGCCGGCGCTGGCGGCG
TGA

Upstream 100 bases:

>100_bases
TTATGGGCTGCTCTCCGGCGTGATGATCGAGGCGGCCGAGGGCGCTGCCGTCCCGGCGGAATGATCTGAGGCCCATGATC
CCCAGCAACGGAATGCGCAC

Downstream 100 bases:

>100_bases
GGAGAGAGACATCATGAGCGGCGACTTTACAGTGGATCAGAAACGCTATCTGGAAGGTTTCATGTCCGGCGCGCAGGTGG
CCCGCGCGGGTCGCGCGCCC

Product: nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSEPLVIIGNGMAATKLCEELGACGLGRYSVAVVGAEPGLAYNRVLLSDVLAGKLPATDLEMKPASWWAKQGVTLLYGTP
AASIDRESRAVVLADGRRLPFSRLVLATGSHPIRLPLPGADLPGVTTFRDLADVSALLAAAKPGVRAVVIGGGLLGLEAA
IGLAGAGVATTLVHLMDRLMERQLDATAADLLRQAVEARGVTVRLQAHSAAIEGESHVTGLRLADGEVLPADLVVMACGV
RPNADLARATGLTCNRGVVVDDHLATSDPAIFALGECAEHRGMAYGLVAPAYEQAHVLARHLAGKPAAYPGSVVSTNLKV
SGVPVFSAGDISGEGAQDIVLRDRAHGIYRRLFIRDGVLAGAVLFGDTADGLYFLDLITNRTPIAPLRAALAFGRPALAA

Sequences:

>Translated_400_residues
MSEPLVIIGNGMAATKLCEELGACGLGRYSVAVVGAEPGLAYNRVLLSDVLAGKLPATDLEMKPASWWAKQGVTLLYGTP
AASIDRESRAVVLADGRRLPFSRLVLATGSHPIRLPLPGADLPGVTTFRDLADVSALLAAAKPGVRAVVIGGGLLGLEAA
IGLAGAGVATTLVHLMDRLMERQLDATAADLLRQAVEARGVTVRLQAHSAAIEGESHVTGLRLADGEVLPADLVVMACGV
RPNADLARATGLTCNRGVVVDDHLATSDPAIFALGECAEHRGMAYGLVAPAYEQAHVLARHLAGKPAAYPGSVVSTNLKV
SGVPVFSAGDISGEGAQDIVLRDRAHGIYRRLFIRDGVLAGAVLFGDTADGLYFLDLITNRTPIAPLRAALAFGRPALAA
>Mature_399_residues
SEPLVIIGNGMAATKLCEELGACGLGRYSVAVVGAEPGLAYNRVLLSDVLAGKLPATDLEMKPASWWAKQGVTLLYGTPA
ASIDRESRAVVLADGRRLPFSRLVLATGSHPIRLPLPGADLPGVTTFRDLADVSALLAAAKPGVRAVVIGGGLLGLEAAI
GLAGAGVATTLVHLMDRLMERQLDATAADLLRQAVEARGVTVRLQAHSAAIEGESHVTGLRLADGEVLPADLVVMACGVR
PNADLARATGLTCNRGVVVDDHLATSDPAIFALGECAEHRGMAYGLVAPAYEQAHVLARHLAGKPAAYPGSVVSTNLKVS
GVPVFSAGDISGEGAQDIVLRDRAHGIYRRLFIRDGVLAGAVLFGDTADGLYFLDLITNRTPIAPLRAALAFGRPALAA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=278, Percent_Identity=26.978417266187, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI65787454, Length=278, Percent_Identity=26.978417266187, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI226437568, Length=278, Percent_Identity=26.978417266187, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI4757732, Length=192, Percent_Identity=28.125, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI22202629, Length=192, Percent_Identity=28.125, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789765, Length=385, Percent_Identity=36.1038961038961, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1789065, Length=337, Percent_Identity=30.8605341246291, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788892, Length=278, Percent_Identity=30.2158273381295, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17559934, Length=284, Percent_Identity=29.2253521126761, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI32564386, Length=285, Percent_Identity=27.3684210526316, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24639257, Length=360, Percent_Identity=27.2222222222222, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281359715, Length=365, Percent_Identity=27.3972602739726, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI281359713, Length=365, Percent_Identity=27.3972602739726, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24639250, Length=365, Percent_Identity=27.3972602739726, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI18543267, Length=365, Percent_Identity=27.3972602739726, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24639252, Length=365, Percent_Identity=27.3972602739726, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24585130, Length=280, Percent_Identity=22.1428571428571, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI28573993, Length=209, Percent_Identity=28.7081339712919, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24581020, Length=209, Percent_Identity=28.7081339712919, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 41352; Mature: 41221

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPLVIIGNGMAATKLCEELGACGLGRYSVAVVGAEPGLAYNRVLLSDVLAGKLPATDL
CCCCEEEEECCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
EMKPASWWAKQGVTLLYGTPAASIDRESRAVVLADGRRLPFSRLVLATGSHPIRLPLPGA
CCCCHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCCCCCHHHEEEECCCCEEEEECCCC
DLPGVTTFRDLADVSALLAAAKPGVRAVVIGGGLLGLEAAIGLAGAGVATTLVHLMDRLM
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHH
ERQLDATAADLLRQAVEARGVTVRLQAHSAAIEGESHVTGLRLADGEVLPADLVVMACGV
HHHHCHHHHHHHHHHHHHCCCEEEEEECCEEECCCCCEEEEEECCCCCCCHHHEEEECCC
RPNADLARATGLTCNRGVVVDDHLATSDPAIFALGECAEHRGMAYGLVAPAYEQAHVLAR
CCCCCHHHHCCCCCCCCEEEECCCCCCCCCEEEECHHHHHCCCEEEECCCCHHHHHHHHH
HLAGKPAAYPGSVVSTNLKVSGVPVFSAGDISGEGAQDIVLRDRAHGIYRRLFIRDGVLA
HHCCCCCCCCCCEEECCEEECCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHCCHHE
GAVLFGDTADGLYFLDLITNRTPIAPLRAALAFGRPALAA
EEEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEPLVIIGNGMAATKLCEELGACGLGRYSVAVVGAEPGLAYNRVLLSDVLAGKLPATDL
CCCEEEEECCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
EMKPASWWAKQGVTLLYGTPAASIDRESRAVVLADGRRLPFSRLVLATGSHPIRLPLPGA
CCCCHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCCCCCHHHEEEECCCCEEEEECCCC
DLPGVTTFRDLADVSALLAAAKPGVRAVVIGGGLLGLEAAIGLAGAGVATTLVHLMDRLM
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHH
ERQLDATAADLLRQAVEARGVTVRLQAHSAAIEGESHVTGLRLADGEVLPADLVVMACGV
HHHHCHHHHHHHHHHHHHCCCEEEEEECCEEECCCCCEEEEEECCCCCCCHHHEEEECCC
RPNADLARATGLTCNRGVVVDDHLATSDPAIFALGECAEHRGMAYGLVAPAYEQAHVLAR
CCCCCHHHHCCCCCCCCEEEECCCCCCCCCEEEECHHHHHCCCEEEECCCCHHHHHHHHH
HLAGKPAAYPGSVVSTNLKVSGVPVFSAGDISGEGAQDIVLRDRAHGIYRRLFIRDGVLA
HHCCCCCCCCCCEEECCEEECCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHCCHHE
GAVLFGDTADGLYFLDLITNRTPIAPLRAALAFGRPALAA
EEEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]