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The map label for this gene is thiE [H]

Identifier: 158422124

GI number: 158422124

Start: 557677

End: 558387

Strand: Reverse

Name: thiE [H]

Synonym: AZC_0500

Alternate gene names: 158422124

Gene position: 558387-557677 (Counterclockwise)

Preceding gene: 158422125

Following gene: 158422123

Centisome position: 10.4

GC content: 72.29

Gene sequence:

>711_bases
ATGCCATCAGGCCCGGCATCCGAAAGGGCCGGGTGCCCCTCTTTCGCCTGCTTTCTGGCCGAACCCGTCATCATGCCCAC
AGAACCGGAAATCCCCCGCTGCCGCCTCATGCTCGTGCTTCCGCCCACCCTGTCGGCGGCGGAAGCCATCGCCTGCATGG
GCGCGGGCGATGTCGCAGCGGTGATCGTCACGACCCCCGCCGGGGATGAAACCGCGGCCAAGGCCCAACTCTCGGAGCTC
TGCACCGCGACGCAGGCCGCGGGCGTCGCCTTCCTTCTGGCCGACCGGGTGGAGCTGGCCTCCGACATCGGCGCCGACGG
CGTTCACCTCGGCAGCTACGCCGCGCTCAAGCGCGCCCTGCCGGCAGTCAAGCCCGGCGGCATCGCCGGCATCGGCCTCG
AAGGCCGGCACGAAGCGATGGAAGCGGGCGAAGCGGGCGCGGATTACGTGCTGTTCGGCGCGCGGGACGGCAGCGGCCCC
ATCGAGCCGGTGGTCGAACTGGTGAGCTGGTGGGCGGAAGTCTTCGAGGTGCCGTGCGTGGGCGTGGCGACCACGGTGGA
GGATGCCCGCGCCATTGCGGTGGCGGGCGCCGATTTCGTGGCGCTGGCGGCCGTGCCCGCCGGTCCGGATGGTCCCGCAC
TCGTTGCGGACGTGGAGCGCGCCGTCGCCGCCGTGGACCGCACCGTGGCCGACGCCACGCCCAAGGCCTGA

Upstream 100 bases:

>100_bases
CTGATCGGCCCCTTGCGCCTTTATCCGCGTTGAGAGAGCCCGCCGTCCCGCGAGGGACCGGCGGGCTCTTGCGTTCTCCC
CCCGGCCCGCCTAGAGCCTG

Downstream 100 bases:

>100_bases
GTGGAAAACGCATGATGGATCGCGTGCTTCGTCTCCTGCGCCCGCTGCTTGGCATTGCGGCGGCCTCCGCCCTCAGTGTC
GCCCTCGCCTTGCCGGCGGC

Product: thiamine-phosphate pyrophosphorylase precursor

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MPSGPASERAGCPSFACFLAEPVIMPTEPEIPRCRLMLVLPPTLSAAEAIACMGAGDVAAVIVTTPAGDETAAKAQLSEL
CTATQAAGVAFLLADRVELASDIGADGVHLGSYAALKRALPAVKPGGIAGIGLEGRHEAMEAGEAGADYVLFGARDGSGP
IEPVVELVSWWAEVFEVPCVGVATTVEDARAIAVAGADFVALAAVPAGPDGPALVADVERAVAAVDRTVADATPKA

Sequences:

>Translated_236_residues
MPSGPASERAGCPSFACFLAEPVIMPTEPEIPRCRLMLVLPPTLSAAEAIACMGAGDVAAVIVTTPAGDETAAKAQLSEL
CTATQAAGVAFLLADRVELASDIGADGVHLGSYAALKRALPAVKPGGIAGIGLEGRHEAMEAGEAGADYVLFGARDGSGP
IEPVVELVSWWAEVFEVPCVGVATTVEDARAIAVAGADFVALAAVPAGPDGPALVADVERAVAAVDRTVADATPKA
>Mature_235_residues
PSGPASERAGCPSFACFLAEPVIMPTEPEIPRCRLMLVLPPTLSAAEAIACMGAGDVAAVIVTTPAGDETAAKAQLSELC
TATQAAGVAFLLADRVELASDIGADGVHLGSYAALKRALPAVKPGGIAGIGLEGRHEAMEAGEAGADYVLFGARDGSGPI
EPVVELVSWWAEVFEVPCVGVATTVEDARAIAVAGADFVALAAVPAGPDGPALVADVERAVAAVDRTVADATPKA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23611; Mature: 23480

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSGPASERAGCPSFACFLAEPVIMPTEPEIPRCRLMLVLPPTLSAAEAIACMGAGDVAA
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCEEE
VIVTTPAGDETAAKAQLSELCTATQAAGVAFLLADRVELASDIGADGVHLGSYAALKRAL
EEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEECHHHHHHHHHC
PAVKPGGIAGIGLEGRHEAMEAGEAGADYVLFGARDGSGPIEPVVELVSWWAEVFEVPCV
CCCCCCCEEECCCCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
GVATTVEDARAIAVAGADFVALAAVPAGPDGPALVADVERAVAAVDRTVADATPKA
CEEEHHHCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PSGPASERAGCPSFACFLAEPVIMPTEPEIPRCRLMLVLPPTLSAAEAIACMGAGDVAA
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCEEE
VIVTTPAGDETAAKAQLSELCTATQAAGVAFLLADRVELASDIGADGVHLGSYAALKRAL
EEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEECHHHHHHHHHC
PAVKPGGIAGIGLEGRHEAMEAGEAGADYVLFGARDGSGPIEPVVELVSWWAEVFEVPCV
CCCCCCCEEECCCCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
GVATTVEDARAIAVAGADFVALAAVPAGPDGPALVADVERAVAAVDRTVADATPKA
CEEEHHHCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA