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The map label for this gene is braE [H]

Identifier: 158422104

GI number: 158422104

Start: 537031

End: 537870

Strand: Reverse

Name: braE [H]

Synonym: AZC_0480

Alternate gene names: 158422104

Gene position: 537870-537031 (Counterclockwise)

Preceding gene: 158422105

Following gene: 158422103

Centisome position: 10.02

GC content: 67.02

Gene sequence:

>840_bases
ATGAGCGGCTATCTCACCGGCGTCCTCGTCGTCCTCTCCTTCAACATCATGGCGGCCTATGCGGTGTATCTGCCGCTCGC
CGCCGGCCAGCTCAATCTCGGCATCGCCGGCTTCATGGCGATCGGCGCCTATGCGGCGGCCTATCTCACCAATGAACTCG
GCTGGTCGCTCTGGACCGCCATTCCCGCTGGCAGCGCGGCGGCCGGCCTCGTGGGGCTCATCATCGGCATCCCGGTGCTG
CGCACCCATGGCATCTATCTGGCGTTGGCCACCTTCGCGCTGGGCCAGGTGATCGCGGCGATCTTCCTCAATCTCGACGT
GGTGGGCGCCGCCGCCGGCTATCCGGTGGCGACCTATGCCGCTCCGCCCGTGGTGTTCGCCGCCACGGCGGGCGTCGTGC
TCGTCATGCTGCTGCTCTCGCGCACCCGCTATGCCCTTTATCTCACCGCCGTGAAGAGCGATCCCACGGTGGCCGATCTC
ATGGGCATCTCGGTGCGCGGCATCCAGGTCTCCGCCTTCGCGCTCGGCGCGGCGGTGGCCGGCTTCGGCGGCGCCTTCTA
CGCGCACCATTACAGCTTCATCGAAGCCCAGCACTTCAACGTGCTGCTCTCGGTCTTCACCGTCCTCTATGTGCTGTTCG
GCGGCGTGCAGACGGTGTGGGGGCCGCTCGCCGGCGCCATCTTCTTCACCCTCGTGCCGGAAGTGCTGCGGGCCTCCGAT
CAGTGGCGCTATGCGCTCTTCGCCTTCTTCATCATCATCTTCATGGCGCTGCGTCCGCAGGGGCTGGTCACCGCTTCGCT
CTTCCGCGTGTTCCGCCGCAAGCCGGGAGCCGCCGCATGA

Upstream 100 bases:

>100_bases
CGGATGTGGTGAACATCACTGTGTACGGCTTGCTGCTCGCCATCCTGATCCTGCGACCGCAAGGTCTCTTCGGCCGGCCG
CCGGCGCGGGAGAAGCTGTG

Downstream 100 bases:

>100_bases
GCACGCCGGCACTCGTCCTCTCCGGCCTCGAGAAGTCCTTCGCCGGGGTGCGAGCCATCCGCAACCTGTCCTTCACCGTG
CCCAAGGGCCGCGCCACGGC

Product: branched-chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSGYLTGVLVVLSFNIMAAYAVYLPLAAGQLNLGIAGFMAIGAYAAAYLTNELGWSLWTAIPAGSAAAGLVGLIIGIPVL
RTHGIYLALATFALGQVIAAIFLNLDVVGAAAGYPVATYAAPPVVFAATAGVVLVMLLLSRTRYALYLTAVKSDPTVADL
MGISVRGIQVSAFALGAAVAGFGGAFYAHHYSFIEAQHFNVLLSVFTVLYVLFGGVQTVWGPLAGAIFFTLVPEVLRASD
QWRYALFAFFIIIFMALRPQGLVTASLFRVFRRKPGAAA

Sequences:

>Translated_279_residues
MSGYLTGVLVVLSFNIMAAYAVYLPLAAGQLNLGIAGFMAIGAYAAAYLTNELGWSLWTAIPAGSAAAGLVGLIIGIPVL
RTHGIYLALATFALGQVIAAIFLNLDVVGAAAGYPVATYAAPPVVFAATAGVVLVMLLLSRTRYALYLTAVKSDPTVADL
MGISVRGIQVSAFALGAAVAGFGGAFYAHHYSFIEAQHFNVLLSVFTVLYVLFGGVQTVWGPLAGAIFFTLVPEVLRASD
QWRYALFAFFIIIFMALRPQGLVTASLFRVFRRKPGAAA
>Mature_278_residues
SGYLTGVLVVLSFNIMAAYAVYLPLAAGQLNLGIAGFMAIGAYAAAYLTNELGWSLWTAIPAGSAAAGLVGLIIGIPVLR
THGIYLALATFALGQVIAAIFLNLDVVGAAAGYPVATYAAPPVVFAATAGVVLVMLLLSRTRYALYLTAVKSDPTVADLM
GISVRGIQVSAFALGAAVAGFGGAFYAHHYSFIEAQHFNVLLSVFTVLYVLFGGVQTVWGPLAGAIFFTLVPEVLRASDQ
WRYALFAFFIIIFMALRPQGLVTASLFRVFRRKPGAAA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=288, Percent_Identity=27.4305555555556, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 29296; Mature: 29165

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SGYLTGVLVVLSFNIMAAYAVYLPLAAGQLNLGIAGFMAIGAYAAAYLTNELGWSLWTA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPAGSAAAGLVGLIIGIPVLRTHGIYLALATFALGQVIAAIFLNLDVVGAAAGYPVATYA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHC
APPVVFAATAGVVLVMLLLSRTRYALYLTAVKSDPTVADLMGISVRGIQVSAFALGAAVA
CCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHCCEECCEEEHHHHHHHHHH
GFGGAFYAHHYSFIEAQHFNVLLSVFTVLYVLFGGVQTVWGPLAGAIFFTLVPEVLRASD
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
QWRYALFAFFIIIFMALRPQGLVTASLFRVFRRKPGAAA
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]