BacMap

Definition	Azorhizobium caulinodans ORS 571, complete genome.
Accession	NC_009937
Length	5,369,772

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The map label for this gene is braF [H]

Identifier: 158422103

GI number: 158422103

Start: 536273

End: 537034

Strand: Reverse

Name: braF [H]

Synonym: AZC_0479

Alternate gene names: 158422103

Gene position: 537034-536273 (Counterclockwise)

Preceding gene: 158422104

Following gene: 158422102

Centisome position: 10.0

GC content: 67.72

Gene sequence:

>762_bases
ATGAGCACGCCGGCACTCGTCCTCTCCGGCCTCGAGAAGTCCTTCGCCGGGGTGCGAGCCATCCGCAACCTGTCCTTCAC
CGTGCCCAAGGGCCGCGCCACGGCCCTGATCGGCCCCAACGGCGCGGGCAAGACCACGGTCTTCAATCTGGTCAGCGGCG
TCTATGGCGTGGACGGCGGGTCCATCCATGTGAACGGCACGGACGTCACGCGCCTGCCCTCCCGCCGCCGCATCCGTGCG
GGCGTCGCGCGCTCGTTCCAGAACATCCGCCTGATGCCGCACCTCTCGGTGCTGGAGAACCTGCTGGTGGGCCAGCATGT
GCGGGCTTCCTCGCTCGCCGCGCTGCTCACGCCCTACCGGATCATTCCCAACCATCGCTGGAAGCGCGAGGCCCGCGCAG
CGCTGGAGGAAAGCGGCCTTGGCCAGTATGCGGACGAGACCGTGGGCGCGCTGCCCTATGGCGTGCGCAAGCGCATCGAT
CTCGTGCGTGCGACGCTCGCCGGTGCCGACGTGCTGATGCTGGACGAGCCGGCCGCCGGCCTCAATCCGAGCGAGACCAA
CGCGCTGCGCGATCATCTCAACGCCCTCGCGGCGCGCGGGCTGACGTTGCTCATCGTCGAGCACGACATGCATTTCGTGG
ACAGCATCTGCGAAAATGTGGTGGTGCTCAATTTCGGCGAGAAGATCGCCGAGGGGCGGCTCTCGGACGTGCGCAAGGAT
CCGAAGGTCCGCGAGGCCTATATCGGCACGGACGAGGAGTGA

Upstream 100 bases:

>100_bases
TCTTCGCCTTCTTCATCATCATCTTCATGGCGCTGCGTCCGCAGGGGCTGGTCACCGCTTCGCTCTTCCGCGTGTTCCGC
CGCAAGCCGGGAGCCGCCGC

Downstream 100 bases:

>100_bases
GGGGCATGGCGCTGCTCGAAGTCTCCGGCCTCGAAGTCGCCTATGGCCGCGTCAACGCGCTGCGGGGCGTGGACCTGCGG
GTGGAGGAAGGCCAGATCGT

Product: ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSTPALVLSGLEKSFAGVRAIRNLSFTVPKGRATALIGPNGAGKTTVFNLVSGVYGVDGGSIHVNGTDVTRLPSRRRIRA
GVARSFQNIRLMPHLSVLENLLVGQHVRASSLAALLTPYRIIPNHRWKREARAALEESGLGQYADETVGALPYGVRKRID
LVRATLAGADVLMLDEPAAGLNPSETNALRDHLNALAARGLTLLIVEHDMHFVDSICENVVVLNFGEKIAEGRLSDVRKD
PKVREAYIGTDEE

Sequences:

>Translated_253_residues
MSTPALVLSGLEKSFAGVRAIRNLSFTVPKGRATALIGPNGAGKTTVFNLVSGVYGVDGGSIHVNGTDVTRLPSRRRIRA
GVARSFQNIRLMPHLSVLENLLVGQHVRASSLAALLTPYRIIPNHRWKREARAALEESGLGQYADETVGALPYGVRKRID
LVRATLAGADVLMLDEPAAGLNPSETNALRDHLNALAARGLTLLIVEHDMHFVDSICENVVVLNFGEKIAEGRLSDVRKD
PKVREAYIGTDEE
>Mature_252_residues
STPALVLSGLEKSFAGVRAIRNLSFTVPKGRATALIGPNGAGKTTVFNLVSGVYGVDGGSIHVNGTDVTRLPSRRRIRAG
VARSFQNIRLMPHLSVLENLLVGQHVRASSLAALLTPYRIIPNHRWKREARAALEESGLGQYADETVGALPYGVRKRIDL
VRATLAGADVLMLDEPAAGLNPSETNALRDHLNALAARGLTLLIVEHDMHFVDSICENVVVLNFGEKIAEGRLSDVRKDP
KVREAYIGTDEE

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=260, Percent_Identity=27.3076923076923, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI27881501, Length=225, Percent_Identity=25.3333333333333, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI30795238, Length=225, Percent_Identity=25.3333333333333, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI150417984, Length=221, Percent_Identity=28.5067873303167, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI105990541, Length=234, Percent_Identity=27.7777777777778, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI47078218, Length=209, Percent_Identity=26.3157894736842, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI45446740, Length=209, Percent_Identity=26.3157894736842, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI21536376, Length=205, Percent_Identity=23.9024390243902, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI27262624, Length=214, Percent_Identity=26.6355140186916, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI27262626, Length=214, Percent_Identity=26.6355140186916, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI27436953, Length=238, Percent_Identity=23.9495798319328, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI27477115, Length=183, Percent_Identity=28.9617486338798, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI116734710, Length=208, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI153792144, Length=166, Percent_Identity=28.3132530120482, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI9955963, Length=232, Percent_Identity=24.1379310344828, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI11967969, Length=223, Percent_Identity=26.0089686098655, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI46592964, Length=222, Percent_Identity=25.2252252252252, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI46592956, Length=222, Percent_Identity=25.2252252252252, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI46592915, Length=222, Percent_Identity=25.2252252252252, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI46592898, Length=222, Percent_Identity=25.2252252252252, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI46592978, Length=222, Percent_Identity=25.2252252252252, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI46592971, Length=222, Percent_Identity=25.2252252252252, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI171184400, Length=251, Percent_Identity=25.8964143426295, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1789864, Length=264, Percent_Identity=39.7727272727273, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1790525, Length=244, Percent_Identity=29.0983606557377, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789593, Length=248, Percent_Identity=31.4516129032258, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1790190, Length=235, Percent_Identity=28.0851063829787, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1787370, Length=241, Percent_Identity=29.8755186721992, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI48995001, Length=219, Percent_Identity=30.5936073059361, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788472, Length=235, Percent_Identity=27.6595744680851, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI87081709, Length=238, Percent_Identity=28.9915966386555, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788210, Length=230, Percent_Identity=28.695652173913, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1789672, Length=238, Percent_Identity=25.6302521008403, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787792, Length=257, Percent_Identity=26.4591439688716, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788761, Length=242, Percent_Identity=29.7520661157025, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1786872, Length=238, Percent_Identity=28.1512605042017, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786398, Length=242, Percent_Identity=29.7520661157025, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1787029, Length=237, Percent_Identity=24.8945147679325, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787712, Length=251, Percent_Identity=27.8884462151394, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1789991, Length=226, Percent_Identity=27.8761061946903, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI87081782, Length=250, Percent_Identity=28, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788225, Length=245, Percent_Identity=26.530612244898, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787089, Length=238, Percent_Identity=27.3109243697479, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790544, Length=230, Percent_Identity=29.5652173913043, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI87081791, Length=255, Percent_Identity=25.8823529411765, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1789032, Length=239, Percent_Identity=27.1966527196653, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788897, Length=219, Percent_Identity=27.8538812785388, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082267, Length=224, Percent_Identity=30.3571428571429, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1786253, Length=231, Percent_Identity=29.004329004329, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI48994943, Length=254, Percent_Identity=25.1968503937008, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788450, Length=226, Percent_Identity=26.5486725663717, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790467, Length=246, Percent_Identity=25.609756097561, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789586, Length=229, Percent_Identity=30.1310043668122, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788506, Length=236, Percent_Identity=28.3898305084746, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI87082268, Length=251, Percent_Identity=24.3027888446215, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786319, Length=226, Percent_Identity=25.6637168141593, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786703, Length=208, Percent_Identity=28.8461538461538, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786563, Length=252, Percent_Identity=26.1904761904762, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787759, Length=265, Percent_Identity=28.6792452830189, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790162, Length=251, Percent_Identity=25.4980079681275, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788053, Length=203, Percent_Identity=32.0197044334975, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787164, Length=226, Percent_Identity=26.9911504424779, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787105, Length=236, Percent_Identity=27.5423728813559, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789962, Length=229, Percent_Identity=24.0174672489083, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1786803, Length=215, Percent_Identity=26.046511627907, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790739, Length=237, Percent_Identity=23.6286919831224, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787143, Length=220, Percent_Identity=27.7272727272727, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789873, Length=215, Percent_Identity=24.6511627906977, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788644, Length=254, Percent_Identity=24.4094488188976, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786345, Length=232, Percent_Identity=25.8620689655172, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87081834, Length=225, Percent_Identity=25.7777777777778, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787758, Length=243, Percent_Identity=25.1028806584362, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=223, Percent_Identity=26.457399103139, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17510237, Length=228, Percent_Identity=27.6315789473684, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71983523, Length=241, Percent_Identity=28.6307053941909, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71983516, Length=241, Percent_Identity=28.6307053941909, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71983510, Length=241, Percent_Identity=28.6307053941909, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115533608, Length=233, Percent_Identity=25.7510729613734, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI115534520, Length=249, Percent_Identity=25.7028112449799, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17541710, Length=215, Percent_Identity=26.046511627907, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6325030, Length=128, Percent_Identity=35.15625, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24666092, Length=243, Percent_Identity=25.5144032921811, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI221512771, Length=226, Percent_Identity=24.3362831858407, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24643674, Length=229, Percent_Identity=25.3275109170306, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28574150, Length=224, Percent_Identity=22.3214285714286, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI116007328, Length=224, Percent_Identity=22.3214285714286, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI28573573, Length=239, Percent_Identity=23.4309623430962, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28573571, Length=239, Percent_Identity=23.4309623430962, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920532, Length=198, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24580930, Length=198, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28574744, Length=190, Percent_Identity=32.6315789473684, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28574533, Length=217, Percent_Identity=30.4147465437788, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648314, Length=234, Percent_Identity=24.7863247863248, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21357907, Length=220, Percent_Identity=26.8181818181818, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27346; Mature: 27215

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPALVLSGLEKSFAGVRAIRNLSFTVPKGRATALIGPNGAGKTTVFNLVSGVYGVDGG
CCCHHHHHHHHHHHHHHHHHHHHCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCC
SIHVNGTDVTRLPSRRRIRAGVARSFQNIRLMPHLSVLENLLVGQHVRASSLAALLTPYR
EEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCHH
IIPNHRWKREARAALEESGLGQYADETVGALPYGVRKRIDLVRATLAGADVLMLDEPAAG
HCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCC
LNPSETNALRDHLNALAARGLTLLIVEHDMHFVDSICENVVVLNFGEKIAEGRLSDVRKD
CCCCHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHCCEEEEECCHHHHHCHHHHHHCC
PKVREAYIGTDEE
CCHHHHCCCCCCC
>Mature Secondary Structure 
STPALVLSGLEKSFAGVRAIRNLSFTVPKGRATALIGPNGAGKTTVFNLVSGVYGVDGG
CCHHHHHHHHHHHHHHHHHHHHCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCC
SIHVNGTDVTRLPSRRRIRAGVARSFQNIRLMPHLSVLENLLVGQHVRASSLAALLTPYR
EEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCHH
IIPNHRWKREARAALEESGLGQYADETVGALPYGVRKRIDLVRATLAGADVLMLDEPAAG
HCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCC
LNPSETNALRDHLNALAARGLTLLIVEHDMHFVDSICENVVVLNFGEKIAEGRLSDVRKD
CCCCHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHCCEEEEECCHHHHHCHHHHHHCC
PKVREAYIGTDEE
CCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]