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The map label for this gene is trmD

Identifier: 158337230

GI number: 158337230

Start: 4144119

End: 4144826

Strand: Reverse

Name: trmD

Synonym: AM1_4106

Alternate gene names: 158337230

Gene position: 4144826-4144119 (Counterclockwise)

Preceding gene: 158337231

Following gene: 158337227

Centisome position: 63.73

GC content: 49.29

Gene sequence:

>708_bases
GTGCGTTTCGATATTCTCACCTTATTTCCAGAATTTTTTACCTCCCCCTTGCAAACTAGTCTGTTGGGGAAAGCCCTTGC
CAATGAGGTTGCAGAGGTCCATCTCACCAATCCTCGCGATTTCACCACGGATAAGCATCATCGGGTTGATGATGAGGTGT
ATGGGGGCGGGGTCGGTATGTTGATGAAGCCCGAACCTATTTTTGCGGCTGTGGAGTCTTTGCCCTGTTTGCCTCGCCGC
CAGATTGTCTTGTTCACCCCCCAGGGGCGCCCCATGGATCAACCGCTATTTCATGAACTCGCGAATCATTACGATCAGCT
GGTGATGATTTGCGGCCATTACGAAGGGGTCGATGAGCGAGTCGCTGAACATTTAGTCACCCAAGAGCTGTCTCTCGGAG
ATTTTGTGCTGACCTGTGGTGAGATTCCAGCGTTAACTCTTTTAAATGGGGTAATTCGTTTGCGGCCAGGTACAGTCGGC
AAGGTCGAATCTTTAAAGAAAGAGAGTTTTGAAACTGAGTTACTTGATTATCCACAATATACTCGACCCGCTGAGTTTCG
GGGATGGCAGGTCCCTTCTGTACTAAGGTCTGGGAACCATGCACTGATAGAGCGGTGGCGACAGGAACAGCAGATCCAAC
GAACTCGAGATCGCAGACCGGATTTGTACCAAAAATGGCTCAAAAAATCTGATACCTCAGATCCTTAA

Upstream 100 bases:

>100_bases
TTGCGACTTATACCTATATAGATGGCTTTGTCCCCTGGCATAGATCGGTGACCATCTTCTTCCCGGCGGTATAGCTGCAG
CGAATCAGGAACTCCCTACC

Downstream 100 bases:

>100_bases
TCTGGAAGTAAACTGGCCTGACAAGGGGCAAAGGACAATCGATGTTGGCGCGTTGGTCCTAGTTCTCGCAACGCTTGCCG
ATGTTTAGCACTGCCATAGC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MRFDILTLFPEFFTSPLQTSLLGKALANEVAEVHLTNPRDFTTDKHHRVDDEVYGGGVGMLMKPEPIFAAVESLPCLPRR
QIVLFTPQGRPMDQPLFHELANHYDQLVMICGHYEGVDERVAEHLVTQELSLGDFVLTCGEIPALTLLNGVIRLRPGTVG
KVESLKKESFETELLDYPQYTRPAEFRGWQVPSVLRSGNHALIERWRQEQQIQRTRDRRPDLYQKWLKKSDTSDP

Sequences:

>Translated_235_residues
MRFDILTLFPEFFTSPLQTSLLGKALANEVAEVHLTNPRDFTTDKHHRVDDEVYGGGVGMLMKPEPIFAAVESLPCLPRR
QIVLFTPQGRPMDQPLFHELANHYDQLVMICGHYEGVDERVAEHLVTQELSLGDFVLTCGEIPALTLLNGVIRLRPGTVG
KVESLKKESFETELLDYPQYTRPAEFRGWQVPSVLRSGNHALIERWRQEQQIQRTRDRRPDLYQKWLKKSDTSDP
>Mature_235_residues
MRFDILTLFPEFFTSPLQTSLLGKALANEVAEVHLTNPRDFTTDKHHRVDDEVYGGGVGMLMKPEPIFAAVESLPCLPRR
QIVLFTPQGRPMDQPLFHELANHYDQLVMICGHYEGVDERVAEHLVTQELSLGDFVLTCGEIPALTLLNGVIRLRPGTVG
KVESLKKESFETELLDYPQYTRPAEFRGWQVPSVLRSGNHALIERWRQEQQIQRTRDRRPDLYQKWLKKSDTSDP

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:
GO:0003723: tRNA (guanine-N(1)-)-methyltransferase
GO:0005737: tRNA (guanine-N(1)-)-methyltransferase
GO:0008033: tRNA (guanine-N(1)-)-methyltransferase
GO:0008168: tRNA (guanine-N(1)-)-methyltransferase
GO:0009019: tRNA (guanine-N(1)-)-methyltransferase
GO:0016740: tRNA (guanine-N(1)-)-methyltransferase
GO:0032259: tRNA (guanine-N(1)-)-methyltransferase

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=227, Percent_Identity=42.7312775330396, Blast_Score=189, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_ACAM1 (B0CAM2)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518405.1
- ProteinModelPortal:   B0CAM2
- SMR:   B0CAM2
- GeneID:   5682911
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4106
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- ProtClustDB:   PRK00026
- BioCyc:   AMAR329726:AM1_4106-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26980; Mature: 26980

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDILTLFPEFFTSPLQTSLLGKALANEVAEVHLTNPRDFTTDKHHRVDDEVYGGGVGM
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHCCCCCCE
LMKPEPIFAAVESLPCLPRRQIVLFTPQGRPMDQPLFHELANHYDQLVMICGHYEGVDER
EECCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
VAEHLVTQELSLGDFVLTCGEIPALTLLNGVIRLRPGTVGKVESLKKESFETELLDYPQY
HHHHHHHHHCCCCHHHEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
TRPAEFRGWQVPSVLRSGNHALIERWRQEQQIQRTRDRRPDLYQKWLKKSDTSDP
CCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRFDILTLFPEFFTSPLQTSLLGKALANEVAEVHLTNPRDFTTDKHHRVDDEVYGGGVGM
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHCCCCCCE
LMKPEPIFAAVESLPCLPRRQIVLFTPQGRPMDQPLFHELANHYDQLVMICGHYEGVDER
EECCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
VAEHLVTQELSLGDFVLTCGEIPALTLLNGVIRLRPGTVGKVESLKKESFETELLDYPQY
HHHHHHHHHCCCCHHHEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
TRPAEFRGWQVPSVLRSGNHALIERWRQEQQIQRTRDRRPDLYQKWLKKSDTSDP
CCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA