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The map label for this gene is argB

Identifier: 158337231

GI number: 158337231

Start: 4144958

End: 4145812

Strand: Reverse

Name: argB

Synonym: AM1_4108

Alternate gene names: 158337231

Gene position: 4145812-4144958 (Counterclockwise)

Preceding gene: 158337232

Following gene: 158337230

Centisome position: 63.75

GC content: 50.53

Gene sequence:

>855_bases
ATGTTTAACGCTAGCGATCGCGTCCAAGTATTAAGTGAGGCTCTTCCTTACATCCAATCCTTCGCTGGACGCACAGTCGT
TGTTAAATATGGTGGGGCGGCCATGAAAGATAGCACCCTTAAAGCCACAGTAATTCGGGATGTGGTGTTTATGGCTTGTG
TTGGTCTACGCCCTGTCCTCGTCCATGGCGGGGGACCGGAAATTAATCTATGGCTGGAAAAGTTGGGAATTGAGCCTCAG
TTTATGAAGGGTTTGCGAGTAACCGATGCACCAACAATGGATGTGGTTGAGATGGTGTTGGTGGGGCGAGTGAATAAAGA
ACTGGTTTCCCTAATTAACCAGGCCGGAGGATCGGCTGTTGGCGTCTGCGGCAAGGACGGCAAGTTACTCACGGCTCGTC
CCCAGGGAGAATCTGGCATTGGGTTTGTAGGAGAGGTGGCCTCAGTAAACCCCCAACTGATCCATAACCTGCTCGATGGG
GGACATATTCCCATCGTGTCCAGTGTGGCGACCGACGAAGAAGGACAGGTCTATAACATTAATGCTGATACCGTCGCGGG
AGAACTCGCCGCTGCGTTAGATGCTGAGAAACTCATTCTACTAACGGATACCCCAGGCATTCTTCAAGACTATAAAGACG
CAACATCCCTCATTCACAAACTGGATATTCGCCAGGCTCGTGAGCTGATAGCAGCAGAGGTTGTAGCTGGAGGCATGATT
CCAAAGGTGACCTGCTGTATTCGCTCTTTAGCCCAAGGGATTCAAGCTGCCCATATTATTGATGGTCGCGCCCCCCATGC
CCTACTGCTAGAAATTTTTACCGATTCTGGGATTGGAACCATGATTACTGCCTAG

Upstream 100 bases:

>100_bases
CTTACGGTCACCTACCATAACGAGCTAAACTGCTGTCTATGGGCAAGCCTTTACTGTTCAATGGTTTGCGCGTTGTTCTC
TTTGTTTGCCACCCAACCCC

Downstream 100 bases:

>100_bases
TTCATCCTCTCCATTTCACACCTATAAATTATTGCGACTTATACCTATATAGATGGCTTTGTCCCCTGGCATAGATCGGT
GACCATCTTCTTCCCGGCGG

Product: acetylglutamate kinase

Products: NA

Alternate protein names: N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; AGK

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MFNASDRVQVLSEALPYIQSFAGRTVVVKYGGAAMKDSTLKATVIRDVVFMACVGLRPVLVHGGGPEINLWLEKLGIEPQ
FMKGLRVTDAPTMDVVEMVLVGRVNKELVSLINQAGGSAVGVCGKDGKLLTARPQGESGIGFVGEVASVNPQLIHNLLDG
GHIPIVSSVATDEEGQVYNINADTVAGELAAALDAEKLILLTDTPGILQDYKDATSLIHKLDIRQARELIAAEVVAGGMI
PKVTCCIRSLAQGIQAAHIIDGRAPHALLLEIFTDSGIGTMITA

Sequences:

>Translated_284_residues
MFNASDRVQVLSEALPYIQSFAGRTVVVKYGGAAMKDSTLKATVIRDVVFMACVGLRPVLVHGGGPEINLWLEKLGIEPQ
FMKGLRVTDAPTMDVVEMVLVGRVNKELVSLINQAGGSAVGVCGKDGKLLTARPQGESGIGFVGEVASVNPQLIHNLLDG
GHIPIVSSVATDEEGQVYNINADTVAGELAAALDAEKLILLTDTPGILQDYKDATSLIHKLDIRQARELIAAEVVAGGMI
PKVTCCIRSLAQGIQAAHIIDGRAPHALLLEIFTDSGIGTMITA
>Mature_284_residues
MFNASDRVQVLSEALPYIQSFAGRTVVVKYGGAAMKDSTLKATVIRDVVFMACVGLRPVLVHGGGPEINLWLEKLGIEPQ
FMKGLRVTDAPTMDVVEMVLVGRVNKELVSLINQAGGSAVGVCGKDGKLLTARPQGESGIGFVGEVASVNPQLIHNLLDG
GHIPIVSSVATDEEGQVYNINADTVAGELAAALDAEKLILLTDTPGILQDYKDATSLIHKLDIRQARELIAAEVVAGGMI
PKVTCCIRSLAQGIQAAHIIDGRAPHALLLEIFTDSGIGTMITA

Specific function: Arginine biosynthesis; first step. [C]

COG id: COG0548

COG function: function code E; Acetylglutamate kinase

Gene ontology:
GO:0000166: Acetylglutamate kinase
GO:0003991: Acetylglutamate kinase
GO:0004349: Acetylglutamate kinase
GO:0005524: Acetylglutamate kinase
GO:0005737: Acetylglutamate kinase
GO:0006526: Acetylglutamate kinase
GO:0006561: Acetylglutamate kinase
GO:0008652: Acetylglutamate kinase
GO:0016301: Acetylglutamate kinase
GO:0016310: Acetylglutamate kinase
GO:0016740: Acetylglutamate kinase

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetylglutamate kinase family

Homologues:

Organism=Escherichia coli, GI1789181, Length=285, Percent_Identity=27.0175438596491, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI145693208, Length=236, Percent_Identity=33.4745762711864, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320913, Length=300, Percent_Identity=30.3333333333333, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ARGB_ACAM1 (B0CAM3)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518406.1
- ProteinModelPortal:   B0CAM3
- SMR:   B0CAM3
- GeneID:   5682912
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4108
- HOGENOM:   HBG497643
- OMA:   MDIVEMV
- ProtClustDB:   PRK00942
- BioCyc:   AMAR329726:AM1_4108-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00082_B
- InterPro:   IPR004662
- InterPro:   IPR001048
- InterPro:   IPR001057
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000728
- PRINTS:   PR00474
- TIGRFAMs:   TIGR00761

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.8

Molecular weight: Translated: 29914; Mature: 29914

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: BINDING 86-86 BINDING 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNASDRVQVLSEALPYIQSFAGRTVVVKYGGAAMKDSTLKATVIRDVVFMACVGLRPVL
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
VHGGGPEINLWLEKLGIEPQFMKGLRVTDAPTMDVVEMVLVGRVNKELVSLINQAGGSAV
EECCCCCEEHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEE
GVCGKDGKLLTARPQGESGIGFVGEVASVNPQLIHNLLDGGHIPIVSSVATDEEGQVYNI
EEECCCCCEEEECCCCCCCCCEECHHHCCCHHHHHHHHCCCCCCEEEECCCCCCCCEEEC
NADTVAGELAAALDAEKLILLTDTPGILQDYKDATSLIHKLDIRQARELIAAEVVAGGMI
CHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
PKVTCCIRSLAQGIQAAHIIDGRAPHALLLEIFTDSGIGTMITA
HHHHHHHHHHHHHHHEEHHCCCCCCHHEEEEEHHCCCCCEEECC
>Mature Secondary Structure
MFNASDRVQVLSEALPYIQSFAGRTVVVKYGGAAMKDSTLKATVIRDVVFMACVGLRPVL
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
VHGGGPEINLWLEKLGIEPQFMKGLRVTDAPTMDVVEMVLVGRVNKELVSLINQAGGSAV
EECCCCCEEHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEE
GVCGKDGKLLTARPQGESGIGFVGEVASVNPQLIHNLLDGGHIPIVSSVATDEEGQVYNI
EEECCCCCEEEECCCCCCCCCEECHHHCCCHHHHHHHHCCCCCCEEEECCCCCCCCEEEC
NADTVAGELAAALDAEKLILLTDTPGILQDYKDATSLIHKLDIRQARELIAAEVVAGGMI
CHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
PKVTCCIRSLAQGIQAAHIIDGRAPHALLLEIFTDSGIGTMITA
HHHHHHHHHHHHHHHEEHHCCCCCCHHEEEEEHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA