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The map label for this gene is rhdA [H]

Identifier: 158335810

GI number: 158335810

Start: 2702753

End: 2703580

Strand: Reverse

Name: rhdA [H]

Synonym: AM1_2661

Alternate gene names: 158335810

Gene position: 2703580-2702753 (Counterclockwise)

Preceding gene: 158335812

Following gene: 158335809

Centisome position: 41.57

GC content: 50.36

Gene sequence:

>828_bases
GTGGTTAGTGCCTTAGAAGCGAAAGTTCTGATCGAACAAGGCGCTACTCTCCTGGATGCCCGAGGGAATCAGTGGTTTGG
GCTCAATCGGTTATCGGGAAGTGTGTCGATACGATGGCAAGATTTTTCTCCCTCTAATAAAGTCCGTAAAGGAACGCTGT
TAGAGAGCGATGCAGTTTTAAATCAGAAATTAAGGAAGCTAGGAATCTCTCAAGAGCGGCCTGTCGTCGTTTTTGCAAAT
CCGCCCCAGGGGTGGGGAGAGGAAGGCCGCATTGTCTGGATGTTAAGAACTCTGGGTCATTCCCAGGCGGTGATGGTGGA
CGGTGGATATTCAGCCTTAGCTTCATTGAACCTGAAGGCCCCAGAAATATCTCAGCTAGGAGATTTTGAGATTCACAGAA
TGCCCACTTGGCAGATTCAGAAAGCAGCCTTAAAAGCGGCACTGGATCAACCCGATTTAGTCTTAATCGATACACGTACT
CCCAAAGAGTATGCTGGTGCAACCCCTTATGGCGAGAAACGGCGAGGACATGTGCCGGGGGCCATTTCACTCCATTTTCA
GGAACTATTAACACCTCAAGGAAAGCTGTTACCCCGAGATCAGATTGTGGCCAAACTCGCTGAGTTGGGGATTACCCAAG
CGTCTGAAGTGGTGGCTTACTGTACTGGAGGGATTCGCTCGGGATGGCTGACGGTGGTTCTGGCGGACTTGGGATATCAG
GTGCAAAATTATGCAGGTTCAATGTGGGAATGGTCTGCAGGGTCTCCGCAAGAGTTTCCGCTGACCGTTCAGTCTCAGGG
GCTAAAGGCGACGGATATGCCACAGTAA

Upstream 100 bases:

>100_bases
GATCGCATTTACCCAAACAACAATCACTACTCCTCATACCCATTCTCCACAGGCTGCTTACACGCTCCGGCCTCAACCTC
CTAAGCTTGAACAAACCTGG

Downstream 100 bases:

>100_bases
AGAGGTGCGATCGCACGCCACACAGATAACCTTAGATATCACACCTATTAATTCCCACTGTTTATACCTAACTTGATACA
TCTGCAGAAACAGTTTGTGG

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MVSALEAKVLIEQGATLLDARGNQWFGLNRLSGSVSIRWQDFSPSNKVRKGTLLESDAVLNQKLRKLGISQERPVVVFAN
PPQGWGEEGRIVWMLRTLGHSQAVMVDGGYSALASLNLKAPEISQLGDFEIHRMPTWQIQKAALKAALDQPDLVLIDTRT
PKEYAGATPYGEKRRGHVPGAISLHFQELLTPQGKLLPRDQIVAKLAELGITQASEVVAYCTGGIRSGWLTVVLADLGYQ
VQNYAGSMWEWSAGSPQEFPLTVQSQGLKATDMPQ

Sequences:

>Translated_275_residues
MVSALEAKVLIEQGATLLDARGNQWFGLNRLSGSVSIRWQDFSPSNKVRKGTLLESDAVLNQKLRKLGISQERPVVVFAN
PPQGWGEEGRIVWMLRTLGHSQAVMVDGGYSALASLNLKAPEISQLGDFEIHRMPTWQIQKAALKAALDQPDLVLIDTRT
PKEYAGATPYGEKRRGHVPGAISLHFQELLTPQGKLLPRDQIVAKLAELGITQASEVVAYCTGGIRSGWLTVVLADLGYQ
VQNYAGSMWEWSAGSPQEFPLTVQSQGLKATDMPQ
>Mature_275_residues
MVSALEAKVLIEQGATLLDARGNQWFGLNRLSGSVSIRWQDFSPSNKVRKGTLLESDAVLNQKLRKLGISQERPVVVFAN
PPQGWGEEGRIVWMLRTLGHSQAVMVDGGYSALASLNLKAPEISQLGDFEIHRMPTWQIQKAALKAALDQPDLVLIDTRT
PKEYAGATPYGEKRRGHVPGAISLHFQELLTPQGKLLPRDQIVAKLAELGITQASEVVAYCTGGIRSGWLTVVLADLGYQ
VQNYAGSMWEWSAGSPQEFPLTVQSQGLKATDMPQ

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Escherichia coli, GI87082121, Length=202, Percent_Identity=25.2475247524752, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17561888, Length=219, Percent_Identity=25.1141552511416, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71997283, Length=221, Percent_Identity=24.8868778280543, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 30115; Mature: 30115

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSALEAKVLIEQGATLLDARGNQWFGLNRLSGSVSIRWQDFSPSNKVRKGTLLESDAVL
CCCCHHHHHHHHCCCEEEECCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCEECHHHHH
NQKLRKLGISQERPVVVFANPPQGWGEEGRIVWMLRTLGHSQAVMVDGGYSALASLNLKA
HHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCEEEEECCHHEEEEECCCC
PEISQLGDFEIHRMPTWQIQKAALKAALDQPDLVLIDTRTPKEYAGATPYGEKRRGHVPG
CCCHHCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCCCCCCCCCCC
AISLHFQELLTPQGKLLPRDQIVAKLAELGITQASEVVAYCTGGIRSGWLTVVLADLGYQ
EEEEEHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHEEEHHHHCHH
VQNYAGSMWEWSAGSPQEFPLTVQSQGLKATDMPQ
HHHHCCCCEECCCCCCCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MVSALEAKVLIEQGATLLDARGNQWFGLNRLSGSVSIRWQDFSPSNKVRKGTLLESDAVL
CCCCHHHHHHHHCCCEEEECCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCEECHHHHH
NQKLRKLGISQERPVVVFANPPQGWGEEGRIVWMLRTLGHSQAVMVDGGYSALASLNLKA
HHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCEEEEECCHHEEEEECCCC
PEISQLGDFEIHRMPTWQIQKAALKAALDQPDLVLIDTRTPKEYAGATPYGEKRRGHVPG
CCCHHCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCCCCCCCCCCC
AISLHFQELLTPQGKLLPRDQIVAKLAELGITQASEVVAYCTGGIRSGWLTVVLADLGYQ
EEEEEHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHEEEHHHHCHH
VQNYAGSMWEWSAGSPQEFPLTVQSQGLKATDMPQ
HHHHCCCCEECCCCCCCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8617271; 10788330 [H]