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The map label for this gene is aroA

Identifier: 158335461

GI number: 158335461

Start: 2307554

End: 2308894

Strand: Direct

Name: aroA

Synonym: AM1_2309

Alternate gene names: 158335461

Gene position: 2307554-2308894 (Clockwise)

Preceding gene: 158335457

Following gene: 158335463

Centisome position: 35.48

GC content: 53.99

Gene sequence:

>1341_bases
ATGCCTACTGATGTGTTGACCGTCTGCCCTGCCCCTACAGGAACAACCTGGGTGATCCAGTCTGAAGGTTATCTTCAGGG
TAAAACGACAATCCCCGGTGATAAGTCCATTTCCCATCGCGCCTTGATGTTGGGCGCTTTGGCCTCCGGTGAAACCACGA
TTCAAGGGCTACTGCTGGGGGAAGATCCTCGTAGCACAGCCCATTGCTTTCGGGCCTTGGGTGCTGAGATTTCTGAATTG
AACACAGAACATGTGCGGGTGCAGGGGATTGGTTTAGGCCATTTACTAGAGCCCGACGTGGTATTAGATGCCGGGAATTC
AGGCACCACTCTACGGCTGATGTTAGGGATTCTCGCCTCCCACCCGGATCGATTATTTACAGTGACGGGGGATACTTCCC
TTTGCAGTCGCCCCATGGGCCGCGTTATCAAACCCCTTCAGCAAATGGGCGCTCATATTTGGGGTCGCCAAAGCGATACC
CTAGCTCCTTTAGCGATTCGAGGCCAGCAGTTACAGCCCATCCACTACCCTTCACCCATCGCATCGGCTCAGGTCAAGTC
TTGCATTATGTTTGCTGGCTTAATGGCCGAGGGCCAAACCACCATTACCGAACCGGCCTTATCTCGTGATCACTCGGAGC
GAATGTTTGCTGCTTTTGGTGCCAATATCATCGTTGATCCGGACACCTGTAGCGTCACGGTGACCGGGCCAGCGCAGCTA
CACGGGCAAGCCATCATCGTACCGGGTGATATCAGCTCAGCCGCTTTTTGGCTAGTAGCAGCGGCTATTGTTCCGGGTTC
AGATATCTGGATTGAAAATGTCGGGGTTAACCCCACTCGCACCGGCGTTCTAGAGGTGCTAGAGCAGATGGGAGCCAAGA
TGACCCTGGCCAACCAACGAACCGTAGCGGGGGAGCCTGTGGCCGATATCCATATCCAGCATAGTCAGCTTCACGGCACT
GTCATTGAGGGAGCTGTGATTCCGCGGTTAGTAGATGAAGTTCCGATTCTGGCCGTTGCAGCCTTGTTTGCCCAAGGACC
TACCACCATCCGAGATGCAGCAGAATTACGGGTCAAGGAAAGCGATCGCTTGACGGCCATGGCCACTCAACTCCAAAAAA
TGGGAGCAAATATCACCGAGCAACCCGATGGATTAGAGATCCAAGGGGGTGCCAGTTTACAGGGAGCAGACGTGGATAGT
TTTGGGGATCACCGCGTGGGGATGAGTCTTGCGATCGCAGCCCTAAACGCTACTGGATCTACCACCATTCACCGGGCAGA
AGCCGCTGCCATTTCATATCCCAATTTTGTAGCGACCTTACAACAGCTTTGGCACTCCTAA

Upstream 100 bases:

>100_bases
CGTCTACTGAACATTGTAAAAGCGATCCAGACCAGACTGCAGCCAATTCCTCTAAACACGATAGGATGGCGATTGGCTGT
TCTTCGGGATTGTAAAGTCT

Downstream 100 bases:

>100_bases
TCCCGTCTTTCCCAATCAGAGAGAGAATCGGTAAGGGGGCCAATCGTTAAGACAGGTCTGCCAGCAGTGAATCCAGTTCA
GACAAGCTGGTTTGCTTACC

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MPTDVLTVCPAPTGTTWVIQSEGYLQGKTTIPGDKSISHRALMLGALASGETTIQGLLLGEDPRSTAHCFRALGAEISEL
NTEHVRVQGIGLGHLLEPDVVLDAGNSGTTLRLMLGILASHPDRLFTVTGDTSLCSRPMGRVIKPLQQMGAHIWGRQSDT
LAPLAIRGQQLQPIHYPSPIASAQVKSCIMFAGLMAEGQTTITEPALSRDHSERMFAAFGANIIVDPDTCSVTVTGPAQL
HGQAIIVPGDISSAAFWLVAAAIVPGSDIWIENVGVNPTRTGVLEVLEQMGAKMTLANQRTVAGEPVADIHIQHSQLHGT
VIEGAVIPRLVDEVPILAVAALFAQGPTTIRDAAELRVKESDRLTAMATQLQKMGANITEQPDGLEIQGGASLQGADVDS
FGDHRVGMSLAIAALNATGSTTIHRAEAAAISYPNFVATLQQLWHS

Sequences:

>Translated_446_residues
MPTDVLTVCPAPTGTTWVIQSEGYLQGKTTIPGDKSISHRALMLGALASGETTIQGLLLGEDPRSTAHCFRALGAEISEL
NTEHVRVQGIGLGHLLEPDVVLDAGNSGTTLRLMLGILASHPDRLFTVTGDTSLCSRPMGRVIKPLQQMGAHIWGRQSDT
LAPLAIRGQQLQPIHYPSPIASAQVKSCIMFAGLMAEGQTTITEPALSRDHSERMFAAFGANIIVDPDTCSVTVTGPAQL
HGQAIIVPGDISSAAFWLVAAAIVPGSDIWIENVGVNPTRTGVLEVLEQMGAKMTLANQRTVAGEPVADIHIQHSQLHGT
VIEGAVIPRLVDEVPILAVAALFAQGPTTIRDAAELRVKESDRLTAMATQLQKMGANITEQPDGLEIQGGASLQGADVDS
FGDHRVGMSLAIAALNATGSTTIHRAEAAAISYPNFVATLQQLWHS
>Mature_445_residues
PTDVLTVCPAPTGTTWVIQSEGYLQGKTTIPGDKSISHRALMLGALASGETTIQGLLLGEDPRSTAHCFRALGAEISELN
TEHVRVQGIGLGHLLEPDVVLDAGNSGTTLRLMLGILASHPDRLFTVTGDTSLCSRPMGRVIKPLQQMGAHIWGRQSDTL
APLAIRGQQLQPIHYPSPIASAQVKSCIMFAGLMAEGQTTITEPALSRDHSERMFAAFGANIIVDPDTCSVTVTGPAQLH
GQAIIVPGDISSAAFWLVAAAIVPGSDIWIENVGVNPTRTGVLEVLEQMGAKMTLANQRTVAGEPVADIHIQHSQLHGTV
IEGAVIPRLVDEVPILAVAALFAQGPTTIRDAAELRVKESDRLTAMATQLQKMGANITEQPDGLEIQGGASLQGADVDSF
GDHRVGMSLAIAALNATGSTTIHRAEAAAISYPNFVATLQQLWHS

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:
GO:0003824: 3-phosphoshikimate 1-carboxyvinyltransferase
GO:0003866: 3-phosphoshikimate 1-carboxyvinyltransferase
GO:0005737: 3-phosphoshikimate 1-carboxyvinyltransferase
GO:0008652: 3-phosphoshikimate 1-carboxyvinyltransferase
GO:0009073: 3-phosphoshikimate 1-carboxyvinyltransferase
GO:0016740: 3-phosphoshikimate 1-carboxyvinyltransferase
GO:0016765: 3-phosphoshikimate 1-carboxyvinyltransferase

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=439, Percent_Identity=28.9293849658314, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320332, Length=431, Percent_Identity=29.2343387470998, Blast_Score=119, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_ACAM1 (B0C218)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516633.1
- ProteinModelPortal:   B0C218
- SMR:   B0C218
- GeneID:   5681122
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2309
- HOGENOM:   HBG646626
- OMA:   LSRNHTE
- BioCyc:   AMAR329726:AM1_2309-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 47035; Mature: 46904

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTDVLTVCPAPTGTTWVIQSEGYLQGKTTIPGDKSISHRALMLGALASGETTIQGLLLG
CCCCEEEECCCCCCCEEEEECCCEEECCCCCCCCCCCCHHEEEEEECCCCCCEEEEEEEC
EDPRSTAHCFRALGAEISELNTEHVRVQGIGLGHLLEPDVVLDAGNSGTTLRLMLGILAS
CCCCHHHHHHHHHCCHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHC
HPDRLFTVTGDTSLCSRPMGRVIKPLQQMGAHIWGRQSDTLAPLAIRGQQLQPIHYPSPI
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCC
ASAQVKSCIMFAGLMAEGQTTITEPALSRDHSERMFAAFGANIIVDPDTCSVTVTGPAQL
HHHHHHHHHHHHHHHCCCCCEECCCHHCCCHHHHHHHHHCCCEEECCCCEEEEECCHHHH
HGQAIIVPGDISSAAFWLVAAAIVPGSDIWIENVGVNPTRTGVLEVLEQMGAKMTLANQR
CCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEECCCC
TVAGEPVADIHIQHSQLHGTVIEGAVIPRLVDEVPILAVAALFAQGPTTIRDAAELRVKE
CCCCCCCEEEEEEEHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECC
SDRLTAMATQLQKMGANITEQPDGLEIQGGASLQGADVDSFGDHRVGMSLAIAALNATGS
CCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TTIHRAEAAAISYPNFVATLQQLWHS
CEEEEHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
PTDVLTVCPAPTGTTWVIQSEGYLQGKTTIPGDKSISHRALMLGALASGETTIQGLLLG
CCCEEEECCCCCCCEEEEECCCEEECCCCCCCCCCCCHHEEEEEECCCCCCEEEEEEEC
EDPRSTAHCFRALGAEISELNTEHVRVQGIGLGHLLEPDVVLDAGNSGTTLRLMLGILAS
CCCCHHHHHHHHHCCHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHC
HPDRLFTVTGDTSLCSRPMGRVIKPLQQMGAHIWGRQSDTLAPLAIRGQQLQPIHYPSPI
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCC
ASAQVKSCIMFAGLMAEGQTTITEPALSRDHSERMFAAFGANIIVDPDTCSVTVTGPAQL
HHHHHHHHHHHHHHHCCCCCEECCCHHCCCHHHHHHHHHCCCEEECCCCEEEEECCHHHH
HGQAIIVPGDISSAAFWLVAAAIVPGSDIWIENVGVNPTRTGVLEVLEQMGAKMTLANQR
CCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEECCCC
TVAGEPVADIHIQHSQLHGTVIEGAVIPRLVDEVPILAVAALFAQGPTTIRDAAELRVKE
CCCCCCCEEEEEEEHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECC
SDRLTAMATQLQKMGANITEQPDGLEIQGGASLQGADVDSFGDHRVGMSLAIAALNATGS
CCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TTIHRAEAAAISYPNFVATLQQLWHS
CEEEEHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA