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The map label for this gene is folC [H]

Identifier: 158334901

GI number: 158334901

Start: 1726596

End: 1727876

Strand: Reverse

Name: folC [H]

Synonym: AM1_1737

Alternate gene names: 158334901

Gene position: 1727876-1726596 (Counterclockwise)

Preceding gene: 158334902

Following gene: 158334899

Centisome position: 26.57

GC content: 52.46

Gene sequence:

>1281_bases
TTGTTTTCATCTTTAATTAGTCGTTACGAGCGATTTGGTATCCATCTGGGCTTAGAGCGGATTCAGTATCTGCTTGATCA
AATTGGCCAACCTCACCTGCAGGTGCCGATCATTCATGTGGCAGGTACGAATGGCAAGGGGTCTGTTTGTGCCTATCTGT
CGTCCGTCTTAACAGCGGCTGGGTATCGAGTCGGCCGCTATACCTCTCCCCATTTGGTGGACTGGACTGAACGAATTTGT
ATTAATAATCAGCCCATTGAGACTTCAGCTCTGACGCAGCTTCTAGTGGATTTAGAGGCGGAGCTGGCCTCGATTGCCTT
GCCGTCCCCTCTCACCCAATTTGAGGTGATTACTGCCCTGGCTTGGCAGTATTTTGCTCAGCAGCAGGTGGATCTGGCGG
TGATTGAAGTGGGCCTGGGGGGACGGTTAGATGCGACGAATGTTTGTGAGCAGCCCTTAGTCAGCGTGATTACCTCATTA
AGTCGCGAACATTGGCAGCGCTTGGGACCGACTCTGGCCGATATCGCTGGGGAAAAGGCAGGGGTTTTGAAGCCTCAGCG
GCCTGCTGTAGTGGGTCTCCTACCCCCCGAAGCTGCAGCAGTAGTCCAAGCCAAAATTCAAAAACTCAATTGTCCGGCGA
TCTGGCCTCAACCGGCTCGGCGAGTTGAAGCTCCCCATGCCACTCCTGATACGTTGCCTTGGGCAGAATCGGCGGGTGTG
AGATTCCCTCTCAGCTTGCCGGGGGAGATTCAATTGACGAATACGGCGGTTGCGATCGCAACCCTCAAACAACTTCAACT
CCAAGGCTGGCAAATTTCAGATGAAGCCATTATCACCGGGATGGCGAATACCCGTTGGCCGGGACGGCTGCAGTGGGTTA
ACTGGCAACAACATCCATTACTGATTGATGGTGCCCATAATGTTGCAGCAGCCCGAGGCTTACGACAGTATGTGGACAGC
CAGCAACCGTCTCAGGTCCATTGGCTCATGGGTATGTTATCCACTAAGGATCACCAAGATATTCTGGAGATTCTTCTACA
GCCAGGGGATACCCTATCTCTCGTTCCAGTTCCCGGCCACAGTAGCGCTGAACCCCAAGCCTTAGCAGAACTAGCGAAGA
CGGTTTGTCCTGGGCTGGCGCGTTGTCAGTGCTTTACCAGCCTAGAAAAAGCATTGAAGGATGCCTTTCGACCTGAGTCT
CAGACATCCTCTATGCATGTGTTATGTGGTTCGTTATATCTCGTAGGTCACTATCTTCAGACCTATGCCCCCACGGTTTG
A

Upstream 100 bases:

>100_bases
TGGTACCCAAAAAAGCAGGACGCAAACCTCGTCCAATTTCCTTGGATGAGTCGGGGCCAGGTCTCTGAGTCCTTCCGTTC
GTTTCTAATGGCTGCTCTCA

Downstream 100 bases:

>100_bases
TTCCAAGCTTTCTCGGCATCGGTGATGGCTTGATCCACCGACTTCTTATCGAGCATGGCCTGCTGCAGATTCTCATAGAG
GATTTTCTGCAGTTTTTTGA

Product: bifunctional folylpolyglutamate synthetase and dihydrofolate synthetase FolC

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MFSSLISRYERFGIHLGLERIQYLLDQIGQPHLQVPIIHVAGTNGKGSVCAYLSSVLTAAGYRVGRYTSPHLVDWTERIC
INNQPIETSALTQLLVDLEAELASIALPSPLTQFEVITALAWQYFAQQQVDLAVIEVGLGGRLDATNVCEQPLVSVITSL
SREHWQRLGPTLADIAGEKAGVLKPQRPAVVGLLPPEAAAVVQAKIQKLNCPAIWPQPARRVEAPHATPDTLPWAESAGV
RFPLSLPGEIQLTNTAVAIATLKQLQLQGWQISDEAIITGMANTRWPGRLQWVNWQQHPLLIDGAHNVAAARGLRQYVDS
QQPSQVHWLMGMLSTKDHQDILEILLQPGDTLSLVPVPGHSSAEPQALAELAKTVCPGLARCQCFTSLEKALKDAFRPES
QTSSMHVLCGSLYLVGHYLQTYAPTV

Sequences:

>Translated_426_residues
MFSSLISRYERFGIHLGLERIQYLLDQIGQPHLQVPIIHVAGTNGKGSVCAYLSSVLTAAGYRVGRYTSPHLVDWTERIC
INNQPIETSALTQLLVDLEAELASIALPSPLTQFEVITALAWQYFAQQQVDLAVIEVGLGGRLDATNVCEQPLVSVITSL
SREHWQRLGPTLADIAGEKAGVLKPQRPAVVGLLPPEAAAVVQAKIQKLNCPAIWPQPARRVEAPHATPDTLPWAESAGV
RFPLSLPGEIQLTNTAVAIATLKQLQLQGWQISDEAIITGMANTRWPGRLQWVNWQQHPLLIDGAHNVAAARGLRQYVDS
QQPSQVHWLMGMLSTKDHQDILEILLQPGDTLSLVPVPGHSSAEPQALAELAKTVCPGLARCQCFTSLEKALKDAFRPES
QTSSMHVLCGSLYLVGHYLQTYAPTV
>Mature_426_residues
MFSSLISRYERFGIHLGLERIQYLLDQIGQPHLQVPIIHVAGTNGKGSVCAYLSSVLTAAGYRVGRYTSPHLVDWTERIC
INNQPIETSALTQLLVDLEAELASIALPSPLTQFEVITALAWQYFAQQQVDLAVIEVGLGGRLDATNVCEQPLVSVITSL
SREHWQRLGPTLADIAGEKAGVLKPQRPAVVGLLPPEAAAVVQAKIQKLNCPAIWPQPARRVEAPHATPDTLPWAESAGV
RFPLSLPGEIQLTNTAVAIATLKQLQLQGWQISDEAIITGMANTRWPGRLQWVNWQQHPLLIDGAHNVAAARGLRQYVDS
QQPSQVHWLMGMLSTKDHQDILEILLQPGDTLSLVPVPGHSSAEPQALAELAKTVCPGLARCQCFTSLEKALKDAFRPES
QTSSMHVLCGSLYLVGHYLQTYAPTV

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=316, Percent_Identity=32.9113924050633, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI66932984, Length=316, Percent_Identity=32.9113924050633, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1788654, Length=411, Percent_Identity=34.0632603406326, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17553150, Length=288, Percent_Identity=32.6388888888889, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17553148, Length=288, Percent_Identity=32.6388888888889, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71984923, Length=288, Percent_Identity=32.6388888888889, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6323760, Length=435, Percent_Identity=34.0229885057471, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6324815, Length=315, Percent_Identity=30.7936507936508, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322718, Length=439, Percent_Identity=26.879271070615, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24641571, Length=354, Percent_Identity=27.1186440677966, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24581568, Length=175, Percent_Identity=34.2857142857143, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 46519; Mature: 46519

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSSLISRYERFGIHLGLERIQYLLDQIGQPHLQVPIIHVAGTNGKGSVCAYLSSVLTAA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHH
GYRVGRYTSPHLVDWTERICINNQPIETSALTQLLVDLEAELASIALPSPLTQFEVITAL
CCCCCCCCCCCHHCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AWQYFAQQQVDLAVIEVGLGGRLDATNVCEQPLVSVITSLSREHWQRLGPTLADIAGEKA
HHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCC
GVLKPQRPAVVGLLPPEAAAVVQAKIQKLNCPAIWPQPARRVEAPHATPDTLPWAESAGV
CCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCC
RFPLSLPGEIQLTNTAVAIATLKQLQLQGWQISDEAIITGMANTRWPGRLQWVNWQQHPL
EEECCCCCCEEECCHHHHHHHHHHHHHCCCEECCCCEEECCCCCCCCCEEEEECCCCCCE
LIDGAHNVAAARGLRQYVDSQQPSQVHWLMGMLSTKDHQDILEILLQPGDTLSLVPVPGH
EEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCC
SSAEPQALAELAKTVCPGLARCQCFTSLEKALKDAFRPESQTSSMHVLCGSLYLVGHYLQ
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
TYAPTV
HHCCCC
>Mature Secondary Structure
MFSSLISRYERFGIHLGLERIQYLLDQIGQPHLQVPIIHVAGTNGKGSVCAYLSSVLTAA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHH
GYRVGRYTSPHLVDWTERICINNQPIETSALTQLLVDLEAELASIALPSPLTQFEVITAL
CCCCCCCCCCCHHCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AWQYFAQQQVDLAVIEVGLGGRLDATNVCEQPLVSVITSLSREHWQRLGPTLADIAGEKA
HHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCC
GVLKPQRPAVVGLLPPEAAAVVQAKIQKLNCPAIWPQPARRVEAPHATPDTLPWAESAGV
CCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCC
RFPLSLPGEIQLTNTAVAIATLKQLQLQGWQISDEAIITGMANTRWPGRLQWVNWQQHPL
EEECCCCCCEEECCHHHHHHHHHHHHHCCCEECCCCEEECCCCCCCCCEEEEECCCCCCE
LIDGAHNVAAARGLRQYVDSQQPSQVHWLMGMLSTKDHQDILEILLQPGDTLSLVPVPGH
EEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCC
SSAEPQALAELAKTVCPGLARCQCFTSLEKALKDAFRPESQTSSMHVLCGSLYLVGHYLQ
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
TYAPTV
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]