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The map label for this gene is lip2 [H]

Identifier: 158334489

GI number: 158334489

Start: 1299071

End: 1300030

Strand: Reverse

Name: lip2 [H]

Synonym: AM1_1313

Alternate gene names: 158334489

Gene position: 1300030-1299071 (Counterclockwise)

Preceding gene: 158334491

Following gene: 158334486

Centisome position: 19.99

GC content: 53.54

Gene sequence:

>960_bases
ATGCCAAAAACTACCATTGATCTTGTTGATCCAGAACTCCGTCCGACAATGGAATCCTTACCCGCATTCAAAATTGGGAC
AGAAACACTTGCCGAGGTGCGGGCTGGGGTCCTGGATTTTGTGCCACCCACAGCAGATTACGAGCGCGACGACGTTAAGA
TGGAGAGCCGCACCATTCCTGGCCTGGAAGGTGGGCCGGACATCGGAATTAACATCTTTACCCCCATAGGCGGCGACGAT
CTCAAGCCCGCAATGCTGCACATTCATGGTGGCGGCTACATCGTTGGAACAGCCGATCTTGGGAATGTAGGCAACACACG
CACGGCATCTGAAGTCGGTTGCGTTGTCGTTTCGGTCGACTACCAGCTGTCTCCAGAAGTAACGGCACCGACCGCAGTCA
AAGAATGCTACACGGCGCTGAAATGGCTGCACGAAAACGCCGACGAGCTTGGTGTGGATCGCTGCCGTATCGCTATCGGT
GGAGAAAGCGCCGGTGGCGGACTGACGGCGAACTTGGCTTTGTACGCCCGAGATTGCGGCGAGATAGACATTTGTTTTCA
GTTGTTAATCTACCCTATGCTCGACGATCGCACAGTGACGTGTTCAGATGGCAATCCGCATGTAGGTGAATTTGGCTGGA
CCCGTGATGCCAACGCCTTTGGCTGGACTTCCTTGCTGGGACAATCACCCGGAGAAGAAGAGGTTCCGCCTTATTCAGTG
GCGGCCCGTGCCACCGATCTTTCGAATCTTCCGCCTGCATACATGTACGTCGGTAGCTTGGATTTGTTCCTTGAAGAAGA
TGTTGAATACGCAATGCGAATGCTCAAAGCGGGCGTTCCTGTAGAGCTTCACGTTCTTGCGGGTGCCTACCACGGCTTTG
AAATGGCCCCGGAAGCAAGGGTCTCAATCAAGGCCGAACAAGAGAGTAGAGACGCACTTAAACGAGTATTCGAACAATAA

Upstream 100 bases:

>100_bases
TTTGAGCCCAACTCACTCTCCCATCAAAGCAGCAACAACCCAACGGGATCGGTCAGATCGACCCCGAATGATGAAACACG
TTATTTTCAGGAGATAGATG

Downstream 100 bases:

>100_bases
CGTTGGCTTTCGGGCAATATATCGCGACTTTCCTCCATGAAAGGCTGCCAAGCCCACCTTATCGGTTCGAAAACGGCGAC
CCAAACATCTGCAACCGGGG

Product: alpha/beta hydrolase domain-containing protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MPKTTIDLVDPELRPTMESLPAFKIGTETLAEVRAGVLDFVPPTADYERDDVKMESRTIPGLEGGPDIGINIFTPIGGDD
LKPAMLHIHGGGYIVGTADLGNVGNTRTASEVGCVVVSVDYQLSPEVTAPTAVKECYTALKWLHENADELGVDRCRIAIG
GESAGGGLTANLALYARDCGEIDICFQLLIYPMLDDRTVTCSDGNPHVGEFGWTRDANAFGWTSLLGQSPGEEEVPPYSV
AARATDLSNLPPAYMYVGSLDLFLEEDVEYAMRMLKAGVPVELHVLAGAYHGFEMAPEARVSIKAEQESRDALKRVFEQ

Sequences:

>Translated_319_residues
MPKTTIDLVDPELRPTMESLPAFKIGTETLAEVRAGVLDFVPPTADYERDDVKMESRTIPGLEGGPDIGINIFTPIGGDD
LKPAMLHIHGGGYIVGTADLGNVGNTRTASEVGCVVVSVDYQLSPEVTAPTAVKECYTALKWLHENADELGVDRCRIAIG
GESAGGGLTANLALYARDCGEIDICFQLLIYPMLDDRTVTCSDGNPHVGEFGWTRDANAFGWTSLLGQSPGEEEVPPYSV
AARATDLSNLPPAYMYVGSLDLFLEEDVEYAMRMLKAGVPVELHVLAGAYHGFEMAPEARVSIKAEQESRDALKRVFEQ
>Mature_318_residues
PKTTIDLVDPELRPTMESLPAFKIGTETLAEVRAGVLDFVPPTADYERDDVKMESRTIPGLEGGPDIGINIFTPIGGDDL
KPAMLHIHGGGYIVGTADLGNVGNTRTASEVGCVVVSVDYQLSPEVTAPTAVKECYTALKWLHENADELGVDRCRIAIGG
ESAGGGLTANLALYARDCGEIDICFQLLIYPMLDDRTVTCSDGNPHVGEFGWTRDANAFGWTSLLGQSPGEEEVPPYSVA
ARATDLSNLPPAYMYVGSLDLFLEEDVEYAMRMLKAGVPVELHVLAGAYHGFEMAPEARVSIKAEQESRDALKRVFEQ

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=168, Percent_Identity=32.1428571428571, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI21328446, Length=96, Percent_Identity=36.4583333333333, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1786682, Length=238, Percent_Identity=26.0504201680672, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI72001146, Length=156, Percent_Identity=28.8461538461538, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71996133, Length=130, Percent_Identity=32.3076923076923, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17540028, Length=123, Percent_Identity=34.9593495934959, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24656084, Length=90, Percent_Identity=35.5555555555556, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24656076, Length=90, Percent_Identity=35.5555555555556, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20130169, Length=90, Percent_Identity=35.5555555555556, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 34410; Mature: 34279

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTTIDLVDPELRPTMESLPAFKIGTETLAEVRAGVLDFVPPTADYERDDVKMESRTIP
CCCCEEEECCCCCCHHHHHCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCC
GLEGGPDIGINIFTPIGGDDLKPAMLHIHGGGYIVGTADLGNVGNTRTASEVGCVVVSVD
CCCCCCCCCEEEECCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCHHHHCEEEEEEC
YQLSPEVTAPTAVKECYTALKWLHENADELGVDRCRIAIGGESAGGGLTANLALYARDCG
CEECCCCCCCHHHHHHHHHHHHHHCCHHHCCCCEEEEEECCCCCCCCEEEEEEEEECCCC
EIDICFQLLIYPMLDDRTVTCSDGNPHVGEFGWTRDANAFGWTSLLGQSPGEEEVPPYSV
CHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCH
AARATDLSNLPPAYMYVGSLDLFLEEDVEYAMRMLKAGVPVELHVLAGAYHGFEMAPEAR
HEHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCE
VSIKAEQESRDALKRVFEQ
EEEEECHHHHHHHHHHHCC
>Mature Secondary Structure 
PKTTIDLVDPELRPTMESLPAFKIGTETLAEVRAGVLDFVPPTADYERDDVKMESRTIP
CCCEEEECCCCCCHHHHHCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCC
GLEGGPDIGINIFTPIGGDDLKPAMLHIHGGGYIVGTADLGNVGNTRTASEVGCVVVSVD
CCCCCCCCCEEEECCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCHHHHCEEEEEEC
YQLSPEVTAPTAVKECYTALKWLHENADELGVDRCRIAIGGESAGGGLTANLALYARDCG
CEECCCCCCCHHHHHHHHHHHHHHCCHHHCCCCEEEEEECCCCCCCCEEEEEEEEECCCC
EIDICFQLLIYPMLDDRTVTCSDGNPHVGEFGWTRDANAFGWTSLLGQSPGEEEVPPYSV
CHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCH
AARATDLSNLPPAYMYVGSLDLFLEEDVEYAMRMLKAGVPVELHVLAGAYHGFEMAPEAR
HEHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCE
VSIKAEQESRDALKRVFEQ
EEEEECHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]