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The map label for this gene is ppsA [H]

Identifier: 158333638

GI number: 158333638

Start: 415999

End: 418524

Strand: Direct

Name: ppsA [H]

Synonym: AM1_0440

Alternate gene names: 158333638

Gene position: 415999-418524 (Clockwise)

Preceding gene: 158333637

Following gene: 158333639

Centisome position: 6.4

GC content: 52.53

Gene sequence:

>2526_bases
ATGAATATTCGTACTGCCCCTCGCCCCACCCCTGAAACCAGCCGTGACCAGGCATTGATCTTAGGTTTTGATGAGGTAGG
CATTGCCGATATCCCCCTAGTGGGCGGCAAAAATGCCTCCCTAGGGGAAATGATCCAGCATTTGGTTCCCCTAGGCGTGA
ACGTCCCCAATGGCTTTGCCACCACCGCCTATGCCTATCGATATTTTGTTCAGCAGGCAGGCATTGAAACCCAACTGCGG
CAGATTTTTGCTGATTTAGATGTTGAGGATGTTGACAATTTACGCCAGCGAGGACGACAGGCGCGATCGCTCATTCTCCA
CACCCCCTTTCCCAAGGATCTGGAGTTAGCGATTTCCACTGCCTATTGCCAACTGTGCGAACAATATGGCGTAGGCAATG
CCGCTTCCGAAGGGCAAGCTTCCAAAGAGCAAGAACGCCTGCAGGCCTATCACTACAACGTTGACGTCGCCGTCCGCTCC
AGTGCCACAGCAGAAGACCTACCCGATGCGAGCTTTGCGGGACAACAGGAAACCTACCTCAATGTCCATGGTGTTAAACA
GGTTTTGGAATCCTGCCACAAATGCTTTGCCTCCATCTTTACAGATCGGGCGATTTCCTATCGCACCCTTAAAGGCTTTG
ACCATTTCCAGGTGGCCCTATCCGTGGGCGTGCAGAAAATGGTGCGATCGGATCTAGCCGCTTCTGGAGTGATGTTCTCC
ATCGATACGGAAACGGGGTTTAAGAACGCAGCCTTGGTCACCGCCGCCTACGGTCTGGGAGAAAATGTGGTTCAAGGCGC
AGTCAACCCCGATGAATTTCTAGTCTTTAAACCGACCTTACTTGACGGCTTTCGTCCCATCCTGAGCAAACATCTGGGCA
GTAAAGAGATCAAAATGGTCTACGATTTGGGGGGTAGCAAACTTACAAAAAATATTGCCGTTCCAGAAGCAGACCGGACC
AAGTTTGCCGTCAACGACGATGAAATCCTCACCTTAGCCAGCTGGGCCTGCATTATTGAAGACCATTATTCCCAGGTCCG
GGGGATGGACACCCCCATGGATATCGAATGGGCCAAGGACGGCCAAACGGGAGTCTTATTTATTGTCCAAGCCCGACCAG
AAACGGTCCAGTCCCAGAAGGTCGCCAATCGACTCTCTGCCTATCGAATGACGGTGGATACTTTGCCTACCCCGTTTATT
GAGGGTCGAGCTGTGGGTGATCGCATTGGCCAGGGGGCAGCTCGGGTGATTGCTTCGGTGCAAGGGATTAAGGAATTCCA
GGCGGGAGAGGTCTTGGTGACCAACCGCACGGACCCCGACTGGGAACCAATTATGAAAAAGGCCAGTGCCATTGTCACCA
ATCAAGGGGGACGCACCTGCCATGCCGCGATTATCGCTCGGGAAATGGGTATTCCCGCCATTGTTGGTTGCAGCACCGCT
ACCGAAGTCCTGAAAACAGGTCAGAATATCACCGTCTCCTGTGCTGAAGGTGAAACGGGACAGGTCTATCCCGGACTGCT
GCCCTTTGTCGTGGAAGAAACCGCCTTAGATGAACTGCCTCCCACCCGAACCCAAATTCTCATGAATGTGGGCAACCCCG
ATGAAGCCTTTGGGCTGGCAGCCATCCCCTGTGATGGGGTGGGGCTAGCGCGATTGGAGTTTATTATCGCCAATCAGATT
AAAACCCATCCTTTGGCGCTGTTGAAATATGACGAGTTGATGGATGTAGAAGCCAAGTTGGCCATTGCTCAACTGACTGC
TGATTACACCTACAAACCGGATTTCTTTATCGATAAGCTGGCCCAAGGGGTGGGCATGATTGCGGCGGCTTTCTATCCGA
ATCCTGTGATCGTCCGCATGTCCGACTTTAAGAGTAATGAATATGCCAACTTAATCGGCGGACGCCAGTATGAACCCACA
GAAGAAAACCCAATGATTGGCTGGCGGGGAGCCTCTCGCTATTACGACCCCAATTACCGTCAAGCCTATGCCCTAGAATG
TCAGGCCCTGAAGAGGGTTCGCGATGAGATGGGACTGCGGAATCTCATTCCCATGATTCCCTTCTGTCGTACTCCTGAAG
AAGGCCAGCAGGTCCTCGAGGAAATGGCCCAGCATGGTCTGAAACGGGGTGAAAACGGCTTGCAGGTTTATGTGATGTGC
GAAGTACCCAGCAATGTGGTGCTGGCAGAGGAATTTGCTGAGGTGTTTGATGGCTTCTCCATCGGCTCCAATGACTTGAC
CCAACTCACCCTAGGACTAGACCGAGACTCCGCCCTTGTTGCCCATATTTTTGATGAGCGTAATGCCGCCGTGAAGAAGT
TGGTGCGCCAAGTAATTGCTGTTGCCAAGGCTAAGGAGCGCAAAATTGGCATCTGTGGTCAGGCCCCCAGTGACTATCCT
GAGTTTGCCCAGTTCTTGGTGGAGGAAGGGATTGACTCCATCAGCCTTAATCCAGATACGGTGCTCAAAACTCGGTTGGC
CATTGCCCAAGTTGAAGACCAATCGACGGGTGTCGTTCAGAACTGA

Upstream 100 bases:

>100_bases
GGTCAGCCGCACCTTTCACAGTCGGTTCTAGCTACCCCTGCTTCCCCTTACCTGACTAAGGGGAAGTTTCCCTCCCTTTC
GAGTTCTCTGGAGGTTTGAG

Downstream 100 bases:

>100_bases
CGGCTAGTCTATGGACCGGACGTCACAAAACTGTAAGGAGAGACCAGATGCCATATCACTCTGTGCCCCATCGAGATCAA
CGGTCACCGTCAGGTGAAAC

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 841; Mature: 841

Protein sequence:

>841_residues
MNIRTAPRPTPETSRDQALILGFDEVGIADIPLVGGKNASLGEMIQHLVPLGVNVPNGFATTAYAYRYFVQQAGIETQLR
QIFADLDVEDVDNLRQRGRQARSLILHTPFPKDLELAISTAYCQLCEQYGVGNAASEGQASKEQERLQAYHYNVDVAVRS
SATAEDLPDASFAGQQETYLNVHGVKQVLESCHKCFASIFTDRAISYRTLKGFDHFQVALSVGVQKMVRSDLAASGVMFS
IDTETGFKNAALVTAAYGLGENVVQGAVNPDEFLVFKPTLLDGFRPILSKHLGSKEIKMVYDLGGSKLTKNIAVPEADRT
KFAVNDDEILTLASWACIIEDHYSQVRGMDTPMDIEWAKDGQTGVLFIVQARPETVQSQKVANRLSAYRMTVDTLPTPFI
EGRAVGDRIGQGAARVIASVQGIKEFQAGEVLVTNRTDPDWEPIMKKASAIVTNQGGRTCHAAIIAREMGIPAIVGCSTA
TEVLKTGQNITVSCAEGETGQVYPGLLPFVVEETALDELPPTRTQILMNVGNPDEAFGLAAIPCDGVGLARLEFIIANQI
KTHPLALLKYDELMDVEAKLAIAQLTADYTYKPDFFIDKLAQGVGMIAAAFYPNPVIVRMSDFKSNEYANLIGGRQYEPT
EENPMIGWRGASRYYDPNYRQAYALECQALKRVRDEMGLRNLIPMIPFCRTPEEGQQVLEEMAQHGLKRGENGLQVYVMC
EVPSNVVLAEEFAEVFDGFSIGSNDLTQLTLGLDRDSALVAHIFDERNAAVKKLVRQVIAVAKAKERKIGICGQAPSDYP
EFAQFLVEEGIDSISLNPDTVLKTRLAIAQVEDQSTGVVQN

Sequences:

>Translated_841_residues
MNIRTAPRPTPETSRDQALILGFDEVGIADIPLVGGKNASLGEMIQHLVPLGVNVPNGFATTAYAYRYFVQQAGIETQLR
QIFADLDVEDVDNLRQRGRQARSLILHTPFPKDLELAISTAYCQLCEQYGVGNAASEGQASKEQERLQAYHYNVDVAVRS
SATAEDLPDASFAGQQETYLNVHGVKQVLESCHKCFASIFTDRAISYRTLKGFDHFQVALSVGVQKMVRSDLAASGVMFS
IDTETGFKNAALVTAAYGLGENVVQGAVNPDEFLVFKPTLLDGFRPILSKHLGSKEIKMVYDLGGSKLTKNIAVPEADRT
KFAVNDDEILTLASWACIIEDHYSQVRGMDTPMDIEWAKDGQTGVLFIVQARPETVQSQKVANRLSAYRMTVDTLPTPFI
EGRAVGDRIGQGAARVIASVQGIKEFQAGEVLVTNRTDPDWEPIMKKASAIVTNQGGRTCHAAIIAREMGIPAIVGCSTA
TEVLKTGQNITVSCAEGETGQVYPGLLPFVVEETALDELPPTRTQILMNVGNPDEAFGLAAIPCDGVGLARLEFIIANQI
KTHPLALLKYDELMDVEAKLAIAQLTADYTYKPDFFIDKLAQGVGMIAAAFYPNPVIVRMSDFKSNEYANLIGGRQYEPT
EENPMIGWRGASRYYDPNYRQAYALECQALKRVRDEMGLRNLIPMIPFCRTPEEGQQVLEEMAQHGLKRGENGLQVYVMC
EVPSNVVLAEEFAEVFDGFSIGSNDLTQLTLGLDRDSALVAHIFDERNAAVKKLVRQVIAVAKAKERKIGICGQAPSDYP
EFAQFLVEEGIDSISLNPDTVLKTRLAIAQVEDQSTGVVQN
>Mature_841_residues
MNIRTAPRPTPETSRDQALILGFDEVGIADIPLVGGKNASLGEMIQHLVPLGVNVPNGFATTAYAYRYFVQQAGIETQLR
QIFADLDVEDVDNLRQRGRQARSLILHTPFPKDLELAISTAYCQLCEQYGVGNAASEGQASKEQERLQAYHYNVDVAVRS
SATAEDLPDASFAGQQETYLNVHGVKQVLESCHKCFASIFTDRAISYRTLKGFDHFQVALSVGVQKMVRSDLAASGVMFS
IDTETGFKNAALVTAAYGLGENVVQGAVNPDEFLVFKPTLLDGFRPILSKHLGSKEIKMVYDLGGSKLTKNIAVPEADRT
KFAVNDDEILTLASWACIIEDHYSQVRGMDTPMDIEWAKDGQTGVLFIVQARPETVQSQKVANRLSAYRMTVDTLPTPFI
EGRAVGDRIGQGAARVIASVQGIKEFQAGEVLVTNRTDPDWEPIMKKASAIVTNQGGRTCHAAIIAREMGIPAIVGCSTA
TEVLKTGQNITVSCAEGETGQVYPGLLPFVVEETALDELPPTRTQILMNVGNPDEAFGLAAIPCDGVGLARLEFIIANQI
KTHPLALLKYDELMDVEAKLAIAQLTADYTYKPDFFIDKLAQGVGMIAAAFYPNPVIVRMSDFKSNEYANLIGGRQYEPT
EENPMIGWRGASRYYDPNYRQAYALECQALKRVRDEMGLRNLIPMIPFCRTPEEGQQVLEEMAQHGLKRGENGLQVYVMC
EVPSNVVLAEEFAEVFDGFSIGSNDLTQLTLGLDRDSALVAHIFDERNAAVKKLVRQVIAVAKAKERKIGICGQAPSDYP
EFAQFLVEEGIDSISLNPDTVLKTRLAIAQVEDQSTGVVQN

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:
GO:0003824: Phosphoenolpyruvate synthase
GO:0005524: Phosphoenolpyruvate synthase
GO:0006094: Phosphoenolpyruvate synthase
GO:0008986: Phosphoenolpyruvate synthase
GO:0016301: Phosphoenolpyruvate synthase
GO:0016310: Phosphoenolpyruvate synthase
GO:0016772: Phosphoenolpyruvate synthase
GO:0016874: Phosphoenolpyruvate synthase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=818, Percent_Identity=54.0342298288509, Blast_Score=877, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=400, Percent_Identity=26.5, Blast_Score=119, Evalue=8e-28,
Organism=Escherichia coli, GI1789193, Length=411, Percent_Identity=24.5742092457421, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1788726, Length=376, Percent_Identity=24.2021276595745, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI48994992, Length=373, Percent_Identity=26.8096514745308, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17564524, Length=103, Percent_Identity=33.9805825242718, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 92113; Mature: 92113

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRTAPRPTPETSRDQALILGFDEVGIADIPLVGGKNASLGEMIQHLVPLGVNVPNGFA
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCH
TTAYAYRYFVQQAGIETQLRQIFADLDVEDVDNLRQRGRQARSLILHTPFPKDLELAIST
HHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHH
AYCQLCEQYGVGNAASEGQASKEQERLQAYHYNVDVAVRSSATAEDLPDASFAGQQETYL
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEECEEEEEECCCCHHCCCCCCCCCCCCCEE
NVHGVKQVLESCHKCFASIFTDRAISYRTLKGFDHFQVALSVGVQKMVRSDLAASGVMFS
EHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
IDTETGFKNAALVTAAYGLGENVVQGAVNPDEFLVFKPTLLDGFRPILSKHLGSKEIKMV
EECCCCCCCCEEEEEECCCCCHHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEE
YDLGGSKLTKNIAVPEADRTKFAVNDDEILTLASWACIIEDHYSQVRGMDTPMDIEWAKD
EECCCCHHCCCCCCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCEEECCC
GQTGVLFIVQARPETVQSQKVANRLSAYRMTVDTLPTPFIEGRAVGDRIGQGAARVIASV
CCCEEEEEEECCCCHHHHHHHHHHHHHHEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
QGIKEFQAGEVLVTNRTDPDWEPIMKKASAIVTNQGGRTCHAAIIAREMGIPAIVGCSTA
HHHHHHCCCCEEEECCCCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCEEEECHHH
TEVLKTGQNITVSCAEGETGQVYPGLLPFVVEETALDELPPTRTQILMNVGNPDEAFGLA
HHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEEECCCCCHHCEEE
AIPCDGVGLARLEFIIANQIKTHPLALLKYDELMDVEAKLAIAQLTADYTYKPDFFIDKL
ECCCCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AQGVGMIAAAFYPNPVIVRMSDFKSNEYANLIGGRQYEPTEENPMIGWRGASRYYDPNYR
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCC
QAYALECQALKRVRDEMGLRNLIPMIPFCRTPEEGQQVLEEMAQHGLKRGENGLQVYVMC
HHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEE
EVPSNVVLAEEFAEVFDGFSIGSNDLTQLTLGLDRDSALVAHIFDERNAAVKKLVRQVIA
ECCCCEEHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHH
VAKAKERKIGICGQAPSDYPEFAQFLVEEGIDSISLNPDTVLKTRLAIAQVEDQSTGVVQ
HHHHHCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCC
N
C
>Mature Secondary Structure
MNIRTAPRPTPETSRDQALILGFDEVGIADIPLVGGKNASLGEMIQHLVPLGVNVPNGFA
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCH
TTAYAYRYFVQQAGIETQLRQIFADLDVEDVDNLRQRGRQARSLILHTPFPKDLELAIST
HHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHH
AYCQLCEQYGVGNAASEGQASKEQERLQAYHYNVDVAVRSSATAEDLPDASFAGQQETYL
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEECEEEEEECCCCHHCCCCCCCCCCCCCEE
NVHGVKQVLESCHKCFASIFTDRAISYRTLKGFDHFQVALSVGVQKMVRSDLAASGVMFS
EHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
IDTETGFKNAALVTAAYGLGENVVQGAVNPDEFLVFKPTLLDGFRPILSKHLGSKEIKMV
EECCCCCCCCEEEEEECCCCCHHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEE
YDLGGSKLTKNIAVPEADRTKFAVNDDEILTLASWACIIEDHYSQVRGMDTPMDIEWAKD
EECCCCHHCCCCCCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCEEECCC
GQTGVLFIVQARPETVQSQKVANRLSAYRMTVDTLPTPFIEGRAVGDRIGQGAARVIASV
CCCEEEEEEECCCCHHHHHHHHHHHHHHEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
QGIKEFQAGEVLVTNRTDPDWEPIMKKASAIVTNQGGRTCHAAIIAREMGIPAIVGCSTA
HHHHHHCCCCEEEECCCCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCEEEECHHH
TEVLKTGQNITVSCAEGETGQVYPGLLPFVVEETALDELPPTRTQILMNVGNPDEAFGLA
HHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEEECCCCCHHCEEE
AIPCDGVGLARLEFIIANQIKTHPLALLKYDELMDVEAKLAIAQLTADYTYKPDFFIDKL
ECCCCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AQGVGMIAAAFYPNPVIVRMSDFKSNEYANLIGGRQYEPTEENPMIGWRGASRYYDPNYR
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCC
QAYALECQALKRVRDEMGLRNLIPMIPFCRTPEEGQQVLEEMAQHGLKRGENGLQVYVMC
HHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEE
EVPSNVVLAEEFAEVFDGFSIGSNDLTQLTLGLDRDSALVAHIFDERNAAVKKLVRQVIA
ECCCCEEHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHH
VAKAKERKIGICGQAPSDYPEFAQFLVEEGIDSISLNPDTVLKTRLAIAQVEDQSTGVVQ
HHHHHCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]