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The map label for this gene is pflA [H]

Identifier: 158333639

GI number: 158333639

Start: 418587

End: 419399

Strand: Direct

Name: pflA [H]

Synonym: AM1_0441

Alternate gene names: 158333639

Gene position: 418587-419399 (Clockwise)

Preceding gene: 158333638

Following gene: 158333640

Centisome position: 6.44

GC content: 52.4

Gene sequence:

>813_bases
GTGCCCCATCGAGATCAACGGTCACCGTCAGGTGAAACAATCTCAGCTCCTGCCTCTCTCCTTGCGGTTTCCTCTCCCAA
TTCTGGTCTGACAGGTCGCATCCATTCTGTAGAAACTTGTGGTAGTGTCGATGGCCCAGGGCTGCGATTTGTAGTGTTTA
TGCAAGGCTGTCCCTTGCGCTGTCTCTACTGCCATAACCCAGACTGCCGAGATGTAACAGGTGGACAGGTGACGACCGTC
GAGGCATTGATAGCAGAGATTCAACGCTATCGATCCTATATGCAGGCATCTGGTGGCGGCGTCACTGTGAGTGGGGGGGA
GCCCTTACTTCAGCCCGAATTTGTTGCGGAACTCATGCGCCAATGTCAGGCCCTAGGCATTCATACCGCCTTAGATACCT
CAGGCTTTTCTGATCTCACCAGCGCTCAGCGGGTACTGCAATATACCGATTTGGTGCTATTAGATATCAAATCCTACGAC
CCTAAACGATTTATCCAAGTGACCCAAGTTTCACGGGAACCTACGCTTTGCCTTGCCCGTTATTTGCATCAAATAGGCAA
ACCCACCTGGATTCGGTTTGTGTTAGTGCCGGGCTTAACAGATGACGTTGAGAATGTGGCCGCGCTCGCCCAGTTCGTAG
CCCATCTCACCAATATTGAACGGGTGGAGGTTCTGCCGTTCCACCAAATGGGGGCCTACAAATGGGAAGAACTGGGCTAT
GACTATCTTTTAAAGGAGACTCAGCCCCCCTCACCTGAGCTAGTTGAGCGGGTTCGCCTACAGTTTCGTGAGTATGGGGT
ATCGGTCCGCTGA

Upstream 100 bases:

>100_bases
AAGTTGAAGACCAATCGACGGGTGTCGTTCAGAACTGACGGCTAGTCTATGGACCGGACGTCACAAAACTGTAAGGAGAG
ACCAGATGCCATATCACTCT

Downstream 100 bases:

>100_bases
CCCCGCTATTTGAATAGGATTGCATTTACCTAAGGAGCACACATGATGACGACTCAAACCCAGCCTCAAGTCACCGATAT
TCCTTCCCTAGAAGATCTGA

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MPHRDQRSPSGETISAPASLLAVSSPNSGLTGRIHSVETCGSVDGPGLRFVVFMQGCPLRCLYCHNPDCRDVTGGQVTTV
EALIAEIQRYRSYMQASGGGVTVSGGEPLLQPEFVAELMRQCQALGIHTALDTSGFSDLTSAQRVLQYTDLVLLDIKSYD
PKRFIQVTQVSREPTLCLARYLHQIGKPTWIRFVLVPGLTDDVENVAALAQFVAHLTNIERVEVLPFHQMGAYKWEELGY
DYLLKETQPPSPELVERVRLQFREYGVSVR

Sequences:

>Translated_270_residues
MPHRDQRSPSGETISAPASLLAVSSPNSGLTGRIHSVETCGSVDGPGLRFVVFMQGCPLRCLYCHNPDCRDVTGGQVTTV
EALIAEIQRYRSYMQASGGGVTVSGGEPLLQPEFVAELMRQCQALGIHTALDTSGFSDLTSAQRVLQYTDLVLLDIKSYD
PKRFIQVTQVSREPTLCLARYLHQIGKPTWIRFVLVPGLTDDVENVAALAQFVAHLTNIERVEVLPFHQMGAYKWEELGY
DYLLKETQPPSPELVERVRLQFREYGVSVR
>Mature_269_residues
PHRDQRSPSGETISAPASLLAVSSPNSGLTGRIHSVETCGSVDGPGLRFVVFMQGCPLRCLYCHNPDCRDVTGGQVTTVE
ALIAEIQRYRSYMQASGGGVTVSGGEPLLQPEFVAELMRQCQALGIHTALDTSGFSDLTSAQRVLQYTDLVLLDIKSYDP
KRFIQVTQVSREPTLCLARYLHQIGKPTWIRFVLVPGLTDDVENVAALAQFVAHLTNIERVEVLPFHQMGAYKWEELGYD
YLLKETQPPSPELVERVRLQFREYGVSVR

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=242, Percent_Identity=46.2809917355372, Blast_Score=241, Evalue=2e-65,
Organism=Escherichia coli, GI1790389, Length=268, Percent_Identity=29.1044776119403, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI226510931, Length=182, Percent_Identity=32.4175824175824, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 29968; Mature: 29836

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHRDQRSPSGETISAPASLLAVSSPNSGLTGRIHSVETCGSVDGPGLRFVVFMQGCPLR
CCCCCCCCCCCCEECCCCEEEEEECCCCCCCEEEECHHHCCCCCCCCEEEEEEECCCCEE
CLYCHNPDCRDVTGGQVTTVEALIAEIQRYRSYMQASGGGVTVSGGEPLLQPEFVAELMR
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHH
QCQALGIHTALDTSGFSDLTSAQRVLQYTDLVLLDIKSYDPKRFIQVTQVSREPTLCLAR
HHHHHCCHHHHCCCCCHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHCCCCCHHHHHH
YLHQIGKPTWIRFVLVPGLTDDVENVAALAQFVAHLTNIERVEVLPFHQMGAYKWEELGY
HHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCHHHCCH
DYLLKETQPPSPELVERVRLQFREYGVSVR
HHEECCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PHRDQRSPSGETISAPASLLAVSSPNSGLTGRIHSVETCGSVDGPGLRFVVFMQGCPLR
CCCCCCCCCCCEECCCCEEEEEECCCCCCCEEEECHHHCCCCCCCCEEEEEEECCCCEE
CLYCHNPDCRDVTGGQVTTVEALIAEIQRYRSYMQASGGGVTVSGGEPLLQPEFVAELMR
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHH
QCQALGIHTALDTSGFSDLTSAQRVLQYTDLVLLDIKSYDPKRFIQVTQVSREPTLCLAR
HHHHHCCHHHHCCCCCHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHCCCCCHHHHHH
YLHQIGKPTWIRFVLVPGLTDDVENVAALAQFVAHLTNIERVEVLPFHQMGAYKWEELGY
HHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCHHHCCH
DYLLKETQPPSPELVERVRLQFREYGVSVR
HHEECCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]