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The map label for this gene is pflB [H]

Identifier: 158333637

GI number: 158333637

Start: 413740

End: 415929

Strand: Direct

Name: pflB [H]

Synonym: AM1_0439

Alternate gene names: 158333637

Gene position: 413740-415929 (Clockwise)

Preceding gene: 158333636

Following gene: 158333638

Centisome position: 6.36

GC content: 53.33

Gene sequence:

>2190_bases
ATGCAAGAGGTAAACGTCCGTGCCTTTATTCAAAGTAACTACACCCCTTATGTCGGGGATGAGACTTTTTTAGCAGCAGC
GAGCGATCGCACCTCGTTACTCTGGGACAAAGTCTCTTTTTCGATGGATGTGGAGCGGCAACAGGGCATTCTGGATGCCG
ACACTCAAGTCCCGGCGGGGATTACGGCCCATGCGCCGGGCTATATTGCTCCTGATCTCGAACAAATTGTGGGCTTACAA
ACGGATCAGCCCCTCAAGCGAGCCATTATGCCCCTGGGGGGAATTCGGGTGGTGCAGAAATCTTTGGAAGCCTATGGCTA
TAAGCTCGATCCAGGCACCCTGAAGACGTTTACCCAATATCGCAAAACCCATAATGATGGGGTATTTGATGCCTATACTC
GCGAGATGCGGTTGGCCCGCCACTCTGGCATTATCACGGGCTTACCTGATGCCTATGGCCGGGGCCGAATTATTGGTGAC
TATCGCCGGGTGGCCCTGTATGGGGTTGATTTCCTGATCAACGATAAGCGACAGCAACTACTGTCTTTGGAAGTAGATGC
GATCAACGAAACCGTAATTCGGCACCGGGAGGAACTCTCGGAGCAGATTCGTGCCCTGCAAGAGCTAAAAGAGATGGCGG
ATCAATATGGGTTTGATATTGGTCAACCCGCCGCCACCGCCCAAGAAGCCGTGCAGTGGACCTACTTTGGCTATCTAGGG
GCGATCAAAGAGCAGAATGGGGCAGCCATGTCCTTAGGCCGAGTCTCCACCTTTTTAGATATTTATATTCAGCGGGATCT
GAACCAAGGCACCCTAACAGAAGCGGAAGCCCAAGAACTAATTGACCATTTTGTGATGAAGTTGCGGATGGTCCGGTTTC
TGCGCACCCCGGCCTATAACGAACTGTTTTCCGGTGATCCCGTATGGGTCACAGAATCGATTGGGGGCATGGGCCTGGAC
GGACGGACCTTGGTCACCCGCACCAGTTTTCGATTCCTCCATACCCTCTATAACCTTGGTCCTGCGCCAGAGCCCAACCT
CACCGTGCTGTGGTCAGAACAACTCCCCGACAACTTTAAGCGCTTCTGTGCACAGGTTTCCATTGATACCAGCTCCATCC
AGTATGAAAACGACGACTTGATGCGAACCGAGTATGGGGATGACTATGGTATTGCCTGCTGTGTGTCCGCTATGCGCATT
GGCAAGCAAATGCAGTTCTTTGGAGCCCGGGTGAATCTGGCGAAAGCGCTGCTGTATGCCATTAACGGCGGCAAGGATGA
AAAATCGGGGGAGCAAATTGCCCCCGCTTATGCGCCGATTACGGCAGACATCCTGGACTACGACGAGGTGATGGCCAAGT
TTGATGTGCTGATGGGCTGGTTAGCCAAGCTCTACGTCAATACCCTCAATGTCATTCACTATATGCATGACAAGTATTGT
TATGAGCGCCTGGAAATGGCCCTCCATGATCGTGATGTGTATCGCACCATGGCCTGCGGCATTGCCGGGCTATCGGTGGT
AGCGGACTCTCTCTCAGCGATCAAACATACCCAAGTCAAGGTGATTCGCGATGACGCGGGACTGGCCATCGATTATAAGT
TAATGGGCAACTACCCGGCCTTTGGCAACAATGACGAACGGGTGGATTCGATTGCCCAGGAGGTGGTGCGGCGGTTTATG
AATCATGTTCGCCAGCATCAAACCTATCGGGATGCAGTACCCACCCAATCCATTCTGACGATTACGTCCAACGTGGTCTA
TGGCAAAAAGACTGGCAGTACCCCCGATGGCCGCAAGGCGGGTGAACCCTTTGGGCCAGGGGCGAACCCCATGCATGGTC
GTGATACGAAGGGTGCGATCGCAGCCTGCGAATCCGTCGCCCAGCTCCCCTATCAACATGCCCAAGACGGCATTTCCTAT
ACCTTTTCCATCGTGCCCCAGGCCTTGGGCAAAGGGGAGACTGACCAAGACCGCAATCTCGTCGGCTTACTGGATGGCTA
CTTCCACAATACGGGCCAGCATATCAACATCAATGTGCTCAACCGTGACACCCTACTGGATGCCATGGAACATCCCGAGC
AGTATCCTCAACTGACCATTCGGGTTTCTGGCTATGCCGTCAACTTTATCAAGCTGACTCGCGAACAACAGCGAGACGTG
GTCAGCCGCACCTTTCACAGTCGGTTCTAG

Upstream 100 bases:

>100_bases
TGAGCAACAATAATTCACTATTTACGATCACTTTGGTGTGATGACTGGAGGTGCCTGCACGATGTTTAAACAGTGGACAG
GTTTTCAGGCCGGGCAGTGG

Downstream 100 bases:

>100_bases
CTACCCCTGCTTCCCCTTACCTGACTAAGGGGAAGTTTCCCTCCCTTTCGAGTTCTCTGGAGGTTTGAGATGAATATTCG
TACTGCCCCTCGCCCCACCC

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 729; Mature: 729

Protein sequence:

>729_residues
MQEVNVRAFIQSNYTPYVGDETFLAAASDRTSLLWDKVSFSMDVERQQGILDADTQVPAGITAHAPGYIAPDLEQIVGLQ
TDQPLKRAIMPLGGIRVVQKSLEAYGYKLDPGTLKTFTQYRKTHNDGVFDAYTREMRLARHSGIITGLPDAYGRGRIIGD
YRRVALYGVDFLINDKRQQLLSLEVDAINETVIRHREELSEQIRALQELKEMADQYGFDIGQPAATAQEAVQWTYFGYLG
AIKEQNGAAMSLGRVSTFLDIYIQRDLNQGTLTEAEAQELIDHFVMKLRMVRFLRTPAYNELFSGDPVWVTESIGGMGLD
GRTLVTRTSFRFLHTLYNLGPAPEPNLTVLWSEQLPDNFKRFCAQVSIDTSSIQYENDDLMRTEYGDDYGIACCVSAMRI
GKQMQFFGARVNLAKALLYAINGGKDEKSGEQIAPAYAPITADILDYDEVMAKFDVLMGWLAKLYVNTLNVIHYMHDKYC
YERLEMALHDRDVYRTMACGIAGLSVVADSLSAIKHTQVKVIRDDAGLAIDYKLMGNYPAFGNNDERVDSIAQEVVRRFM
NHVRQHQTYRDAVPTQSILTITSNVVYGKKTGSTPDGRKAGEPFGPGANPMHGRDTKGAIAACESVAQLPYQHAQDGISY
TFSIVPQALGKGETDQDRNLVGLLDGYFHNTGQHININVLNRDTLLDAMEHPEQYPQLTIRVSGYAVNFIKLTREQQRDV
VSRTFHSRF

Sequences:

>Translated_729_residues
MQEVNVRAFIQSNYTPYVGDETFLAAASDRTSLLWDKVSFSMDVERQQGILDADTQVPAGITAHAPGYIAPDLEQIVGLQ
TDQPLKRAIMPLGGIRVVQKSLEAYGYKLDPGTLKTFTQYRKTHNDGVFDAYTREMRLARHSGIITGLPDAYGRGRIIGD
YRRVALYGVDFLINDKRQQLLSLEVDAINETVIRHREELSEQIRALQELKEMADQYGFDIGQPAATAQEAVQWTYFGYLG
AIKEQNGAAMSLGRVSTFLDIYIQRDLNQGTLTEAEAQELIDHFVMKLRMVRFLRTPAYNELFSGDPVWVTESIGGMGLD
GRTLVTRTSFRFLHTLYNLGPAPEPNLTVLWSEQLPDNFKRFCAQVSIDTSSIQYENDDLMRTEYGDDYGIACCVSAMRI
GKQMQFFGARVNLAKALLYAINGGKDEKSGEQIAPAYAPITADILDYDEVMAKFDVLMGWLAKLYVNTLNVIHYMHDKYC
YERLEMALHDRDVYRTMACGIAGLSVVADSLSAIKHTQVKVIRDDAGLAIDYKLMGNYPAFGNNDERVDSIAQEVVRRFM
NHVRQHQTYRDAVPTQSILTITSNVVYGKKTGSTPDGRKAGEPFGPGANPMHGRDTKGAIAACESVAQLPYQHAQDGISY
TFSIVPQALGKGETDQDRNLVGLLDGYFHNTGQHININVLNRDTLLDAMEHPEQYPQLTIRVSGYAVNFIKLTREQQRDV
VSRTFHSRF
>Mature_729_residues
MQEVNVRAFIQSNYTPYVGDETFLAAASDRTSLLWDKVSFSMDVERQQGILDADTQVPAGITAHAPGYIAPDLEQIVGLQ
TDQPLKRAIMPLGGIRVVQKSLEAYGYKLDPGTLKTFTQYRKTHNDGVFDAYTREMRLARHSGIITGLPDAYGRGRIIGD
YRRVALYGVDFLINDKRQQLLSLEVDAINETVIRHREELSEQIRALQELKEMADQYGFDIGQPAATAQEAVQWTYFGYLG
AIKEQNGAAMSLGRVSTFLDIYIQRDLNQGTLTEAEAQELIDHFVMKLRMVRFLRTPAYNELFSGDPVWVTESIGGMGLD
GRTLVTRTSFRFLHTLYNLGPAPEPNLTVLWSEQLPDNFKRFCAQVSIDTSSIQYENDDLMRTEYGDDYGIACCVSAMRI
GKQMQFFGARVNLAKALLYAINGGKDEKSGEQIAPAYAPITADILDYDEVMAKFDVLMGWLAKLYVNTLNVIHYMHDKYC
YERLEMALHDRDVYRTMACGIAGLSVVADSLSAIKHTQVKVIRDDAGLAIDYKLMGNYPAFGNNDERVDSIAQEVVRRFM
NHVRQHQTYRDAVPTQSILTITSNVVYGKKTGSTPDGRKAGEPFGPGANPMHGRDTKGAIAACESVAQLPYQHAQDGISY
TFSIVPQALGKGETDQDRNLVGLLDGYFHNTGQHININVLNRDTLLDAMEHPEQYPQLTIRVSGYAVNFIKLTREQQRDV
VSRTFHSRF

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=734, Percent_Identity=66.6212534059946, Blast_Score=1036, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=735, Percent_Identity=64.6258503401361, Blast_Score=986, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=579, Percent_Identity=28.6701208981002, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI1790388, Length=669, Percent_Identity=24.813153961136, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1788933, Length=54, Percent_Identity=74.0740740740741, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 81807; Mature: 81807

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEVNVRAFIQSNYTPYVGDETFLAAASDRTSLLWDKVSFSMDVERQQGILDADTQVPAG
CCCCCEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCCC
ITAHAPGYIAPDLEQIVGLQTDQPLKRAIMPLGGIRVVQKSLEAYGYKLDPGTLKTFTQY
CEECCCCCCCCCHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHCCEECCCCHHHHHHHH
RKTHNDGVFDAYTREMRLARHSGIITGLPDAYGRGRIIGDYRRVALYGVDFLINDKRQQL
HHHCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCEEHHHHEEHHEEEHHEECCHHHHH
LSLEVDAINETVIRHREELSEQIRALQELKEMADQYGFDIGQPAATAQEAVQWTYFGYLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AIKEQNGAAMSLGRVSTFLDIYIQRDLNQGTLTEAEAQELIDHFVMKLRMVRFLRTPAYN
HHCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
ELFSGDPVWVTESIGGMGLDGRTLVTRTSFRFLHTLYNLGPAPEPNLTVLWSEQLPDNFK
HHCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHH
RFCAQVSIDTSSIQYENDDLMRTEYGDDYGIACCVSAMRIGKQMQFFGARVNLAKALLYA
HHHHHHCCCCCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INGGKDEKSGEQIAPAYAPITADILDYDEVMAKFDVLMGWLAKLYVNTLNVIHYMHDKYC
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YERLEMALHDRDVYRTMACGIAGLSVVADSLSAIKHTQVKVIRDDAGLAIDYKLMGNYPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEEEEECCCCC
FGNNDERVDSIAQEVVRRFMNHVRQHQTYRDAVPTQSILTITSNVVYGKKTGSTPDGRKA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCEEECCCCCCCCCCCCC
GEPFGPGANPMHGRDTKGAIAACESVAQLPYQHAQDGISYTFSIVPQALGKGETDQDRNL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCE
VGLLDGYFHNTGQHININVLNRDTLLDAMEHPEQYPQLTIRVSGYAVNFIKLTREQQRDV
EHHHHHHHCCCCCEEEEEEECHHHHHHHHHCCCCCCEEEEEEECEEEEHHHHHHHHHHHH
VSRTFHSRF
HHHHHHCCC
>Mature Secondary Structure
MQEVNVRAFIQSNYTPYVGDETFLAAASDRTSLLWDKVSFSMDVERQQGILDADTQVPAG
CCCCCEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCCC
ITAHAPGYIAPDLEQIVGLQTDQPLKRAIMPLGGIRVVQKSLEAYGYKLDPGTLKTFTQY
CEECCCCCCCCCHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHCCEECCCCHHHHHHHH
RKTHNDGVFDAYTREMRLARHSGIITGLPDAYGRGRIIGDYRRVALYGVDFLINDKRQQL
HHHCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCEEHHHHEEHHEEEHHEECCHHHHH
LSLEVDAINETVIRHREELSEQIRALQELKEMADQYGFDIGQPAATAQEAVQWTYFGYLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AIKEQNGAAMSLGRVSTFLDIYIQRDLNQGTLTEAEAQELIDHFVMKLRMVRFLRTPAYN
HHCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
ELFSGDPVWVTESIGGMGLDGRTLVTRTSFRFLHTLYNLGPAPEPNLTVLWSEQLPDNFK
HHCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHH
RFCAQVSIDTSSIQYENDDLMRTEYGDDYGIACCVSAMRIGKQMQFFGARVNLAKALLYA
HHHHHHCCCCCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INGGKDEKSGEQIAPAYAPITADILDYDEVMAKFDVLMGWLAKLYVNTLNVIHYMHDKYC
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YERLEMALHDRDVYRTMACGIAGLSVVADSLSAIKHTQVKVIRDDAGLAIDYKLMGNYPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEEEEECCCCC
FGNNDERVDSIAQEVVRRFMNHVRQHQTYRDAVPTQSILTITSNVVYGKKTGSTPDGRKA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCEEECCCCCCCCCCCCC
GEPFGPGANPMHGRDTKGAIAACESVAQLPYQHAQDGISYTFSIVPQALGKGETDQDRNL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCE
VGLLDGYFHNTGQHININVLNRDTLLDAMEHPEQYPQLTIRVSGYAVNFIKLTREQQRDV
EHHHHHHHCCCCCEEEEEEECHHHHHHHHHCCCCCCEEEEEEECEEEEHHHHHHHHHHHH
VSRTFHSRF
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA