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The map label for this gene is nifH

Identifier: 158318613

GI number: 158318613

Start: 8386074

End: 8386943

Strand: Reverse

Name: nifH

Synonym: Franean1_6881

Alternate gene names: 158318613

Gene position: 8386943-8386074 (Counterclockwise)

Preceding gene: 158318619

Following gene: 158318612

Centisome position: 93.37

GC content: 64.37

Gene sequence:

>870_bases
ATGCGCCAGATCGCGTTCTACGGCAAGGGTGGTATTGGCAAGTCCACCACCCAGCAGAACACCATGGCTGCCATGGCCGA
GATGGGCCGTCGCGTCATGATCGTCGGCTGCGACCCCAAGGCTGACTCGACCCGCCTCATCCTGCACTCGAAGGCCCAGA
CGTCCGTCATCCAGCTCGCGGCGGAGAAGGGCTCGGTCGAGGACCTGGAGCTGGACGAGGTCCTGGTCGAGGGCCAGTGG
GGCATCAAGTGCGTCGAGTCCGGTGGCCCGGAGCCGGGCGTGGGCTGCGCCGGCCGTGGCGTCATCACCTCCATCACCTA
CCTGGAGGAGGCCGGCGCCTACGAGAACCTCGACTTCGTCACCTACGACGTCCTCGGCGACGTTGTCTGCGGTGGCTTCG
CGATGCCGATCCGCCAGGGCAAGGCCCAGGAGATCTACATCGTGACCTCCGGCGAGATGATGGCGATGTACGCGGCGAAC
AACATCGCCCGCGGTGTCCTCAAGTACGCGCACTCCGGCGGTGTCCGCCTCGGTGGGCTCATCTGCAACAGCCGTAACAC
CGACCGCGAGGACGAGCTGATCATCGAGCTCGCCCGCCGTCTCAACACCCAGATGATCCACTTCATCCCGCGTAACAACG
TCGTGCAGCACGCCGAGCTGCGCCGGATGACGGTCATCGAGTACGACCCCGAGAACAGCCAGGCCAACGAGTACCGCCAG
CTGGCCAAGAAGATCGACGAGAACGACATGAAGACCATCCCCACCCCGATCAGCATGGACGAGCTCGAGGAACTGCTGAT
CGAGTTCGGGATCATGGAGCAGGAAGACGAGTCGATCATCGGCCAGAAGGCCAACGCGACCGTCGCCTGA

Upstream 100 bases:

>100_bases
CCCAGTCGGTTCGCGGGGCCACGACCGCAACCGATTTCCGAACCGCGGGACGCACTGCACGAAACCGTGCGTGCGTACGC
AGCTCCAGGAGGAGAATGCA

Downstream 100 bases:

>100_bases
GGCTCCGGCCACCTGCTCTGACACTGTCGAGGATGAGGTCCCGATCATGACGACGACTCCGGCTCCGCAGCGGGCCGAGA
CCGAGGCGATGATCGCCGAG

Product: nitrogenase iron protein

Products: NA

Alternate protein names: Nitrogenase Fe protein; Nitrogenase component II; Nitrogenase reductase

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRQIAFYGKGGIGKSTTQQNTMAAMAEMGRRVMIVGCDPKADSTRLILHSKAQTSVIQLAAEKGSVEDLELDEVLVEGQW
GIKCVESGGPEPGVGCAGRGVITSITYLEEAGAYENLDFVTYDVLGDVVCGGFAMPIRQGKAQEIYIVTSGEMMAMYAAN
NIARGVLKYAHSGGVRLGGLICNSRNTDREDELIIELARRLNTQMIHFIPRNNVVQHAELRRMTVIEYDPENSQANEYRQ
LAKKIDENDMKTIPTPISMDELEELLIEFGIMEQEDESIIGQKANATVA

Sequences:

>Translated_289_residues
MRQIAFYGKGGIGKSTTQQNTMAAMAEMGRRVMIVGCDPKADSTRLILHSKAQTSVIQLAAEKGSVEDLELDEVLVEGQW
GIKCVESGGPEPGVGCAGRGVITSITYLEEAGAYENLDFVTYDVLGDVVCGGFAMPIRQGKAQEIYIVTSGEMMAMYAAN
NIARGVLKYAHSGGVRLGGLICNSRNTDREDELIIELARRLNTQMIHFIPRNNVVQHAELRRMTVIEYDPENSQANEYRQ
LAKKIDENDMKTIPTPISMDELEELLIEFGIMEQEDESIIGQKANATVA
>Mature_289_residues
MRQIAFYGKGGIGKSTTQQNTMAAMAEMGRRVMIVGCDPKADSTRLILHSKAQTSVIQLAAEKGSVEDLELDEVLVEGQW
GIKCVESGGPEPGVGCAGRGVITSITYLEEAGAYENLDFVTYDVLGDVVCGGFAMPIRQGKAQEIYIVTSGEMMAMYAAN
NIARGVLKYAHSGGVRLGGLICNSRNTDREDELIIELARRLNTQMIHFIPRNNVVQHAELRRMTVIEYDPENSQANEYRQ
LAKKIDENDMKTIPTPISMDELEELLIEFGIMEQEDESIIGQKANATVA

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFH_FRASN (A8L2C4)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001511121.1
- ProteinModelPortal:   A8L2C4
- SMR:   A8L2C4
- GeneID:   5675194
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_6881
- HOGENOM:   HBG488165
- OMA:   NDKLTIP
- ProtClustDB:   CLSK949120
- BioCyc:   FSP1855:FRANEAN1_6881-MONOMER
- HAMAP:   MF_00533
- InterPro:   IPR000392
- InterPro:   IPR005977
- PIRSF:   PIRSF000363
- PRINTS:   PR00091
- TIGRFAMs:   TIGR01287

Pfam domain/function: PF00142 Fer4_NifH

EC number: =1.18.6.1

Molecular weight: Translated: 31726; Mature: 31726

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00746 NIFH_FRXC_1; PS00692 NIFH_FRXC_2; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQIAFYGKGGIGKSTTQQNTMAAMAEMGRRVMIVGCDPKADSTRLILHSKAQTSVIQLA
CCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHH
AEKGSVEDLELDEVLVEGQWGIKCVESGGPEPGVGCAGRGVITSITYLEEAGAYENLDFV
HCCCCCCCCCHHHEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEE
TYDVLGDVVCGGFAMPIRQGKAQEIYIVTSGEMMAMYAANNIARGVLKYAHSGGVRLGGL
EHHHHHHHHHCCEECHHCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHCCCCEEEEE
ICNSRNTDREDELIIELARRLNTQMIHFIPRNNVVQHAELRRMTVIEYDPENSQANEYRQ
EECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCEEEEEEECCCCCCHHHHHH
LAKKIDENDMKTIPTPISMDELEELLIEFGIMEQEDESIIGQKANATVA
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MRQIAFYGKGGIGKSTTQQNTMAAMAEMGRRVMIVGCDPKADSTRLILHSKAQTSVIQLA
CCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHH
AEKGSVEDLELDEVLVEGQWGIKCVESGGPEPGVGCAGRGVITSITYLEEAGAYENLDFV
HCCCCCCCCCHHHEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEE
TYDVLGDVVCGGFAMPIRQGKAQEIYIVTSGEMMAMYAANNIARGVLKYAHSGGVRLGGL
EHHHHHHHHHCCEECHHCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHCCCCEEEEE
ICNSRNTDREDELIIELARRLNTQMIHFIPRNNVVQHAELRRMTVIEYDPENSQANEYRQ
EECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCEEEEEEECCCCCCHHHHHH
LAKKIDENDMKTIPTPISMDELEELLIEFGIMEQEDESIIGQKANATVA
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA