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The map label for this gene is nifD [H]

Identifier: 158318612

GI number: 158318612

Start: 8384567

End: 8386027

Strand: Reverse

Name: nifD [H]

Synonym: Franean1_6880

Alternate gene names: 158318612

Gene position: 8386027-8384567 (Counterclockwise)

Preceding gene: 158318613

Following gene: 158318611

Centisome position: 93.36

GC content: 65.85

Gene sequence:

>1461_bases
ATGACGACGACTCCGGCTCCGCAGCGGGCCGAGACCGAGGCGATGATCGCCGAGGTCCTGGAGCAGTACCCCCAGAAGGC
GGCCAAGTTCCGCGCCAAGCACCTCAAGGCCAACGACCCCGAGGGGTCGAAGGAGTGCGAGGTCAAGTCCAACATCAAGT
CCCGCCCCGGGGTCATGACGATCCGTGGCTGTGCCTACGCCGGTTCCAAGGGCGTGGTGTGGGGCCCGGTGAAGGACCTG
GTCACGATCAGCCACGGCCCGGTCGGCTGCGGCCAGTACTCCTGGGCCACCCGGCGCAACTACGCCCGCGGTCCGTGGGG
TGTCACCAACTTCACCGCGATGCAGATCACCACGGACTTCCAGGAGAAGGACATCGTCTTCGGCGGTGACCCCAAGCTGG
AGCAGGTCTGCGACGAGATCAACGAGCTCTTCCCGCTGGCCAAGGGCATCTCGGTCCAGTCCGAGTGCCCGATCGGTCTG
ATCGGCGACGACATCGAGGCGGTTTCCCGCACGGCGTCGAAGAAGCTTGGCAAGCCGGTCATCCCGGTCCGCTGTGAGGG
CTTCCGCGGGGTGAGCCAGTCGCTCGGCCACCACATCGCCAACGACGCGGTCCGCGACCACGTTCTCGGCACCGGCGGCG
AGTCCTTCAAGGAGACCCCGTACGACGTCGCGCTCATCGGCGACTACAACATCGGCGGCGACGCCTGGGCGTCCCGGCGG
ATCCTCGAGGACATGGGCCTGCGCGTCATCGCCCAGTGGTCCGGCGACGGCACGCTGAACGAGATGGCCTCGACGCACCT
GTCGAAGCTGAACCTGATCCACTGCTACCGCTCCATGAACTACATCTGCACCACCATGGAGGAGCGTTACGGCACTCCGT
GGATCGAGTTCAACTTCTTCGGGCCGACCAAGATCGTCAACTCGATGCGGGCCATCGCGGCCAAGTTCGACGAGACGATC
CAGGCGAAGACCGAGGCCGCGATCGCGCGTTACCAGAAGCGCTTCGACGAGATCACGGCGGCGTTCAAGCCGCGCCTCGA
CGGCAAGCGCGTCATGCTCGCTGTCGGCGGCCTGCGTCCCCGTCACACCATCGGCGCCTACGAGGACCTCGGCATGGAGG
TCGTCGGCACCGGTTACGAGTTCGCGCACAAGGACGACTACACCCGCACGTACGCCGAGCTCAAGGAAGGCGTCGTGCTC
TACGACGACCCGACGGCGTTCGAGCTGGAGGAGTTCGCCAAGCGGCTCAAGCCGGACCTCATGGGTGCGGGCGTCAAGGA
GAAGTACGTCTTCCACAAGATGGGCATCCCCTTCCGCCAGATGCACTCCTGGGACTACTCCGGGCCGTACCACGGCGTCG
ACGGCTTCGCGGTCTTCGCCCGCGACATGGACATCGCGATCAACAGCCCGACCTGGGACCTCATGGAGACCCCCTGGTCG
AAGGCCGGAGAGGTGTTCTGA

Upstream 100 bases:

>100_bases
GAGCAGGAAGACGAGTCGATCATCGGCCAGAAGGCCAACGCGACCGTCGCCTGAGGCTCCGGCCACCTGCTCTGACACTG
TCGAGGATGAGGTCCCGATC

Downstream 100 bases:

>100_bases
CCGGATTTTCCGGTCTCCGCCGGCGATCGCCGGCGGCCATCAGTCACGGTAGAAAGGAGGATGTCCGGTGACGACGACTC
CCGAGACAGGCAGTTCCATT

Product: nitrogenase molybdenum-iron protein subunit alpha

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTTTPAPQRAETEAMIAEVLEQYPQKAAKFRAKHLKANDPEGSKECEVKSNIKSRPGVMTIRGCAYAGSKGVVWGPVKDL
VTISHGPVGCGQYSWATRRNYARGPWGVTNFTAMQITTDFQEKDIVFGGDPKLEQVCDEINELFPLAKGISVQSECPIGL
IGDDIEAVSRTASKKLGKPVIPVRCEGFRGVSQSLGHHIANDAVRDHVLGTGGESFKETPYDVALIGDYNIGGDAWASRR
ILEDMGLRVIAQWSGDGTLNEMASTHLSKLNLIHCYRSMNYICTTMEERYGTPWIEFNFFGPTKIVNSMRAIAAKFDETI
QAKTEAAIARYQKRFDEITAAFKPRLDGKRVMLAVGGLRPRHTIGAYEDLGMEVVGTGYEFAHKDDYTRTYAELKEGVVL
YDDPTAFELEEFAKRLKPDLMGAGVKEKYVFHKMGIPFRQMHSWDYSGPYHGVDGFAVFARDMDIAINSPTWDLMETPWS
KAGEVF

Sequences:

>Translated_486_residues
MTTTPAPQRAETEAMIAEVLEQYPQKAAKFRAKHLKANDPEGSKECEVKSNIKSRPGVMTIRGCAYAGSKGVVWGPVKDL
VTISHGPVGCGQYSWATRRNYARGPWGVTNFTAMQITTDFQEKDIVFGGDPKLEQVCDEINELFPLAKGISVQSECPIGL
IGDDIEAVSRTASKKLGKPVIPVRCEGFRGVSQSLGHHIANDAVRDHVLGTGGESFKETPYDVALIGDYNIGGDAWASRR
ILEDMGLRVIAQWSGDGTLNEMASTHLSKLNLIHCYRSMNYICTTMEERYGTPWIEFNFFGPTKIVNSMRAIAAKFDETI
QAKTEAAIARYQKRFDEITAAFKPRLDGKRVMLAVGGLRPRHTIGAYEDLGMEVVGTGYEFAHKDDYTRTYAELKEGVVL
YDDPTAFELEEFAKRLKPDLMGAGVKEKYVFHKMGIPFRQMHSWDYSGPYHGVDGFAVFARDMDIAINSPTWDLMETPWS
KAGEVF
>Mature_485_residues
TTTPAPQRAETEAMIAEVLEQYPQKAAKFRAKHLKANDPEGSKECEVKSNIKSRPGVMTIRGCAYAGSKGVVWGPVKDLV
TISHGPVGCGQYSWATRRNYARGPWGVTNFTAMQITTDFQEKDIVFGGDPKLEQVCDEINELFPLAKGISVQSECPIGLI
GDDIEAVSRTASKKLGKPVIPVRCEGFRGVSQSLGHHIANDAVRDHVLGTGGESFKETPYDVALIGDYNIGGDAWASRRI
LEDMGLRVIAQWSGDGTLNEMASTHLSKLNLIHCYRSMNYICTTMEERYGTPWIEFNFFGPTKIVNSMRAIAAKFDETIQ
AKTEAAIARYQKRFDEITAAFKPRLDGKRVMLAVGGLRPRHTIGAYEDLGMEVVGTGYEFAHKDDYTRTYAELKEGVVLY
DDPTAFELEEFAKRLKPDLMGAGVKEKYVFHKMGIPFRQMHSWDYSGPYHGVDGFAVFARDMDIAINSPTWDLMETPWSK
AGEVF

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 54045; Mature: 53914

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPAPQRAETEAMIAEVLEQYPQKAAKFRAKHLKANDPEGSKECEVKSNIKSRPGVMT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCEEE
IRGCAYAGSKGVVWGPVKDLVTISHGPVGCGQYSWATRRNYARGPWGVTNFTAMQITTDF
EEEEEECCCCCCEECCHHHHEEECCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEEEECC
QEKDIVFGGDPKLEQVCDEINELFPLAKGISVQSECPIGLIGDDIEAVSRTASKKLGKPV
CCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCC
IPVRCEGFRGVSQSLGHHIANDAVRDHVLGTGGESFKETPYDVALIGDYNIGGDAWASRR
CEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
ILEDMGLRVIAQWSGDGTLNEMASTHLSKLNLIHCYRSMNYICTTMEERYGTPWIEFNFF
HHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GPTKIVNSMRAIAAKFDETIQAKTEAAIARYQKRFDEITAAFKPRLDGKRVMLAVGGLRP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
RHTIGAYEDLGMEVVGTGYEFAHKDDYTRTYAELKEGVVLYDDPTAFELEEFAKRLKPDL
CCCCCCHHHCCCEEEECCCHHCCCCCHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCHHH
MGAGVKEKYVFHKMGIPFRQMHSWDYSGPYHGVDGFAVFARDMDIAINSPTWDLMETPWS
HCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEEECCEEEECCCCCHHHHCCCC
KAGEVF
CCCCCC
>Mature Secondary Structure 
TTTPAPQRAETEAMIAEVLEQYPQKAAKFRAKHLKANDPEGSKECEVKSNIKSRPGVMT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCEEE
IRGCAYAGSKGVVWGPVKDLVTISHGPVGCGQYSWATRRNYARGPWGVTNFTAMQITTDF
EEEEEECCCCCCEECCHHHHEEECCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEEEECC
QEKDIVFGGDPKLEQVCDEINELFPLAKGISVQSECPIGLIGDDIEAVSRTASKKLGKPV
CCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCC
IPVRCEGFRGVSQSLGHHIANDAVRDHVLGTGGESFKETPYDVALIGDYNIGGDAWASRR
CEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
ILEDMGLRVIAQWSGDGTLNEMASTHLSKLNLIHCYRSMNYICTTMEERYGTPWIEFNFF
HHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GPTKIVNSMRAIAAKFDETIQAKTEAAIARYQKRFDEITAAFKPRLDGKRVMLAVGGLRP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
RHTIGAYEDLGMEVVGTGYEFAHKDDYTRTYAELKEGVVLYDDPTAFELEEFAKRLKPDL
CCCCCCHHHCCCEEEECCCHHCCCCCHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCHHH
MGAGVKEKYVFHKMGIPFRQMHSWDYSGPYHGVDGFAVFARDMDIAINSPTWDLMETPWS
HCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEEECCEEEECCCCCHHHHCCCC
KAGEVF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1584016 [H]