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The map label for this gene is nifK [H]

Identifier: 158318611

GI number: 158318611

Start: 8382943

End: 8384499

Strand: Reverse

Name: nifK [H]

Synonym: Franean1_6879

Alternate gene names: 158318611

Gene position: 8384499-8382943 (Counterclockwise)

Preceding gene: 158318612

Following gene: 158318610

Centisome position: 93.35

GC content: 67.57

Gene sequence:

>1557_bases
GTGACGACGACTCCCGAGACAGGCAGTTCCATTCCGTTGCGGGTCCTCGACCACAGCGAGCTGTTCAAGGACGAGGTCTA
CCAGAAGCAGTTCGAGGGAAAGACCGAGTTCGAGAACGGCAGTGACTCCGCCGAGGTTGCCCGCGTCCTCGAGTGGACCC
GCGGCTGGGAGTACCGGGAGAAGAACTTCGCCCGGGAGGCGCTGACCGTCAACCCGGCGAAGGCCTGCCAGCCGCTCGGT
GCGGTGCTCGCGGGCCTCGGGTTCCAGGGCACGCTGCCGCTCGTGCACGGTTCGCAGGGCTGCGTCGCGTACTTCCGCAG
CCACTTCGCTCGGCACTTCAAGGAGCCCGTCCCCGCGGCATCCACGTCGATGACCGAGGACGCGGCGGTCTTCGGCGGCC
TGAACAACCTGGTCGAGGCGCTGGAGAACGCGACCAGCCTGTACAAGCCGAAGATGGTCGCGGTCAGCACCACCTGCATG
GCCGAGGTCATCGGTGAGGACCTCTTCGCCTACATCGGCGCGGCCAAGGAGAAGGAGGTGATCTCCACCGACTACCCGGT
TCCCTACGCCCACACCCCGAGCTTCGTGGGCTCGCACATCACCGGGTACGACAGCATGCTCAAGGGAATCCTTGAGAACC
TGACGAAGTCGGCGGACGCGACGGAGCCGAAGGCTGGTGGGAAGCCCCGGCTGAACATCATCCCCGGTTTCGAGACCTAC
ACCGGTAACCTCCGCGAGTACCGGCGCGTGCTCGAGCTCATGGGCGTGGACCCGCTGATCCTCGGCGACCACGCCGACTC
GCTCGACTCGCCGGCCGACGGGGAGTACGACCTCTACCCCGGTGGCACGCCGCTGGCCGAGGCGGCGAAGGCGAAGTTCA
GCCGCGCCACCGTGCTGCTGCAGGAGTCCGCCACCCGCAAGACCACCGAGCTGATCCGGGACGTGTGGAAGCAGGACACG
CTGGTGCTGGAGACCCCGATCGGGGTCCGCGGCACCGACCAGTTCCTGACCGAGATCGCCCGGCTGGCGGGCGTCGAGAT
CCCGGCCGAGCTCACCGTCGAGCGCGGCCGTCTCGTTGACGCCCTGACGGACTCGCACGCCTACCTCCACGGCAAGAGGG
TCGCCATCGCCGGCGACCCGGACCTCGTCGTGGCGCTGACCCGCTTCGTGCTCGAGCTCGGCATGATCCCGGTGCACGTG
CTCAGCACGAACGCCGACACCACCTTCAAGGCCCGCATGGAGAAGGTGCTCTCGGCGAGCAAGTTCGGCGAGGCGGCCAC
CGTCTGGCCGGAGAAGGACCTGTGGCACCTGCGGTCGCTGGTCTTCACCGAGCCGGTCGACCTGCTCATCGGCAGCACCT
ACCTGAAGTACATCTCCCGGGAGGCGAACGTTCCGCTGGTGCGGGTCGGGTTCCCGATCTTCGACCGGCACCACCTGCAC
CGCTTCCCGATCGTCGGTTACACCGGCGGGCTGCACCTGCTCACGCAGCTCGTGAACACCGTGCTGGACGAGCTTGACCG
GACCAGCCCGGACCATAGCTACGACGCCGTGCGCTAG

Upstream 100 bases:

>100_bases
ACCCCCTGGTCGAAGGCCGGAGAGGTGTTCTGACCGGATTTTCCGGTCTCCGCCGGCGATCGCCGGCGGCCATCAGTCAC
GGTAGAAAGGAGGATGTCCG

Downstream 100 bases:

>100_bases
GCCCCGTCCGGGGGACGCCTCCTGAGCGTCCGGTGTCCCCCGGGCGGCGCGCACGGCCTTCCCAGTGGTCGGCCGGCTGG
CGGCCCCGCGCCCCCGCTCC

Product: nitrogenase molybdenum-iron protein subunit beta

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MTTTPETGSSIPLRVLDHSELFKDEVYQKQFEGKTEFENGSDSAEVARVLEWTRGWEYREKNFAREALTVNPAKACQPLG
AVLAGLGFQGTLPLVHGSQGCVAYFRSHFARHFKEPVPAASTSMTEDAAVFGGLNNLVEALENATSLYKPKMVAVSTTCM
AEVIGEDLFAYIGAAKEKEVISTDYPVPYAHTPSFVGSHITGYDSMLKGILENLTKSADATEPKAGGKPRLNIIPGFETY
TGNLREYRRVLELMGVDPLILGDHADSLDSPADGEYDLYPGGTPLAEAAKAKFSRATVLLQESATRKTTELIRDVWKQDT
LVLETPIGVRGTDQFLTEIARLAGVEIPAELTVERGRLVDALTDSHAYLHGKRVAIAGDPDLVVALTRFVLELGMIPVHV
LSTNADTTFKARMEKVLSASKFGEAATVWPEKDLWHLRSLVFTEPVDLLIGSTYLKYISREANVPLVRVGFPIFDRHHLH
RFPIVGYTGGLHLLTQLVNTVLDELDRTSPDHSYDAVR

Sequences:

>Translated_518_residues
MTTTPETGSSIPLRVLDHSELFKDEVYQKQFEGKTEFENGSDSAEVARVLEWTRGWEYREKNFAREALTVNPAKACQPLG
AVLAGLGFQGTLPLVHGSQGCVAYFRSHFARHFKEPVPAASTSMTEDAAVFGGLNNLVEALENATSLYKPKMVAVSTTCM
AEVIGEDLFAYIGAAKEKEVISTDYPVPYAHTPSFVGSHITGYDSMLKGILENLTKSADATEPKAGGKPRLNIIPGFETY
TGNLREYRRVLELMGVDPLILGDHADSLDSPADGEYDLYPGGTPLAEAAKAKFSRATVLLQESATRKTTELIRDVWKQDT
LVLETPIGVRGTDQFLTEIARLAGVEIPAELTVERGRLVDALTDSHAYLHGKRVAIAGDPDLVVALTRFVLELGMIPVHV
LSTNADTTFKARMEKVLSASKFGEAATVWPEKDLWHLRSLVFTEPVDLLIGSTYLKYISREANVPLVRVGFPIFDRHHLH
RFPIVGYTGGLHLLTQLVNTVLDELDRTSPDHSYDAVR
>Mature_517_residues
TTTPETGSSIPLRVLDHSELFKDEVYQKQFEGKTEFENGSDSAEVARVLEWTRGWEYREKNFAREALTVNPAKACQPLGA
VLAGLGFQGTLPLVHGSQGCVAYFRSHFARHFKEPVPAASTSMTEDAAVFGGLNNLVEALENATSLYKPKMVAVSTTCMA
EVIGEDLFAYIGAAKEKEVISTDYPVPYAHTPSFVGSHITGYDSMLKGILENLTKSADATEPKAGGKPRLNIIPGFETYT
GNLREYRRVLELMGVDPLILGDHADSLDSPADGEYDLYPGGTPLAEAAKAKFSRATVLLQESATRKTTELIRDVWKQDTL
VLETPIGVRGTDQFLTEIARLAGVEIPAELTVERGRLVDALTDSHAYLHGKRVAIAGDPDLVVALTRFVLELGMIPVHVL
STNADTTFKARMEKVLSASKFGEAATVWPEKDLWHLRSLVFTEPVDLLIGSTYLKYISREANVPLVRVGFPIFDRHHLHR
FPIVGYTGGLHLLTQLVNTVLDELDRTSPDHSYDAVR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 56945; Mature: 56814

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPETGSSIPLRVLDHSELFKDEVYQKQFEGKTEFENGSDSAEVARVLEWTRGWEYRE
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
KNFAREALTVNPAKACQPLGAVLAGLGFQGTLPLVHGSQGCVAYFRSHFARHFKEPVPAA
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCC
STSMTEDAAVFGGLNNLVEALENATSLYKPKMVAVSTTCMAEVIGEDLFAYIGAAKEKEV
CCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCH
ISTDYPVPYAHTPSFVGSHITGYDSMLKGILENLTKSADATEPKAGGKPRLNIIPGFETY
HCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHC
TGNLREYRRVLELMGVDPLILGDHADSLDSPADGEYDLYPGGTPLAEAAKAKFSRATVLL
CCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHEEH
QESATRKTTELIRDVWKQDTLVLETPIGVRGTDQFLTEIARLAGVEIPAELTVERGRLVD
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCHHHH
ALTDSHAYLHGKRVAIAGDPDLVVALTRFVLELGMIPVHVLSTNADTTFKARMEKVLSAS
HHHCCCCEEECCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHH
KFGEAATVWPEKDLWHLRSLVFTEPVDLLIGSTYLKYISREANVPLVRVGFPIFDRHHLH
HCCCCEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHCCCCCC
RFPIVGYTGGLHLLTQLVNTVLDELDRTSPDHSYDAVR
CCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TTTPETGSSIPLRVLDHSELFKDEVYQKQFEGKTEFENGSDSAEVARVLEWTRGWEYRE
CCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
KNFAREALTVNPAKACQPLGAVLAGLGFQGTLPLVHGSQGCVAYFRSHFARHFKEPVPAA
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCC
STSMTEDAAVFGGLNNLVEALENATSLYKPKMVAVSTTCMAEVIGEDLFAYIGAAKEKEV
CCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCH
ISTDYPVPYAHTPSFVGSHITGYDSMLKGILENLTKSADATEPKAGGKPRLNIIPGFETY
HCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHC
TGNLREYRRVLELMGVDPLILGDHADSLDSPADGEYDLYPGGTPLAEAAKAKFSRATVLL
CCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHEEH
QESATRKTTELIRDVWKQDTLVLETPIGVRGTDQFLTEIARLAGVEIPAELTVERGRLVD
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCHHHH
ALTDSHAYLHGKRVAIAGDPDLVVALTRFVLELGMIPVHVLSTNADTTFKARMEKVLSAS
HHHCCCCEEECCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHH
KFGEAATVWPEKDLWHLRSLVFTEPVDLLIGSTYLKYISREANVPLVRVGFPIFDRHHLH
HCCCCEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHCCCCCC
RFPIVGYTGGLHLLTQLVNTVLDELDRTSPDHSYDAVR
CCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8574444 [H]