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The map label for this gene is nifE [H]

Identifier: 158318610

GI number: 158318610

Start: 8381242

End: 8382615

Strand: Reverse

Name: nifE [H]

Synonym: Franean1_6878

Alternate gene names: 158318610

Gene position: 8382615-8381242 (Counterclockwise)

Preceding gene: 158318611

Following gene: 158318609

Centisome position: 93.33

GC content: 71.91

Gene sequence:

>1374_bases
ATGGCCGCCACTGACCGTGCAACGCTATTCACCGAACCGGCATGTGATCACAACCGCGAGAAGTCCGCCAAGGAGCGCAA
GGCCGGGTGCCCCAAGCCCGCGCCCGGCGGCACCAGCGGCGGATGCACCTTCGACGGTGCGATGATCACACTGGTTCCGA
TCGTCGACAGCGCCCACGTCGTGCACGGCCCGATCGCCTGCGCAGGAAACTCCTGGGACGGGCGGGGGAGCCTCTCCTCC
GGCCCCGACCTCTACCGCCGCGGCTTCACCAGCGACGTCGGTGAGCAGGACGTCATCTTCGGCGGCGAGCAGCGCCTGTT
CGACACCATTCTCGAAGCCGTCCGCCGGCACCACCCGCCGGCGGTGTTCGTCTACTCGACCTGCGTCACCGCCATGATCG
GCGACGACCTCGACGCCGTCTGCTCGGCGGCCGCCGAGCACACCGGCGTCCCGGTCATCCCGGTGCACGCTCCGGGGTTC
GCCGGCAACAAGAACCTCGGCAACCGCCTCGCCGGCGAGGCCCTGCTCGAGCACGTCATCGGGACGGTCGAGCCGCCCGA
CGTGACCGAGCTCGACGTGAACCTGGTCGGCGAGTACAACATCGCCGGCGAGCTGTGGGACGTCCTGCCGGTGCTGGCCA
AGATGGGCATCCGGGTCCGGGCCTGCATCAGCGGCGACGCGCGCTACGCGGACGTCGCCGCGGCGCACCGGGCCCGCGCG
ACGATGGTCGTCTGCTCCCGGGCGCTGCTGGGCCTGGCCCGCGGCCTGGAGGATCGCTACGGCATCCCCTGGTTCGAGGG
CAGCTTCTACGGCGTCCGCGCGATGAACGACACCCTGCGCGAGTTCGCCCGCCTGCTCGGCGGCGCGGAGCTGGCCCGGC
GCGCCGAGGAGGTCATCGCCCTCGAGCAGACCGCCGTCGACCTGGCCCTGGAGCCCTACCGCGAGCGGCTCGCCGGCAGG
CGCGCGGTGCTCTACACCGGCGGGGTCAAGAGCTGGTCGATCGTCTCCGCGCTGCAGGACCTCGGCATCGAGGTCGTCGC
GAACGGCATCACCAAGAGCTCCGACGGCGACGTCGAGAAGATCCGCGAACTGCTCGGCCCGGACGCCAGGATCGTCTCCG
AGGGCAGCCCGCGCGAGCTGCTGCGCATCGCCGAGGAGACCCGCGCGGACATCCTCGTCGCCGGCGGCCGCAACCAGTAC
ACGGCGCTCAAGGGCCGGCTGCCGTTCCTCGACATCAACCAGGAGCGGCACATCCCCTACGCGGGCTACCGCGGCGCGGT
CGAGCTGGCCCGCCGCCTCGACATGGCGCTGTCGAACCCGGTGTGGGAGCAGGTCCGCGCCCCCGCGCCCTGGGACGTCG
AGGGGGTGGCGTGA

Upstream 100 bases:

>100_bases
CCGCCAGCACCACCCCGGTTCAGAAGAACTGCTGGTCCATCGAACGGCCCGCTCACCCAGCTCACCGAGGGGACGACCCT
CCCTAAGCCGAGAGATCGCC

Downstream 100 bases:

>100_bases
TGGCGAGGGTCGTCACGAGTGACCGCCGGCCCGGCCTGGACCCGCTGCGGTTCAGCCAGCCGCTCGGCGGGGCACTGGTC
TTCCTCGGCCTCGCCGCGGC

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MAATDRATLFTEPACDHNREKSAKERKAGCPKPAPGGTSGGCTFDGAMITLVPIVDSAHVVHGPIACAGNSWDGRGSLSS
GPDLYRRGFTSDVGEQDVIFGGEQRLFDTILEAVRRHHPPAVFVYSTCVTAMIGDDLDAVCSAAAEHTGVPVIPVHAPGF
AGNKNLGNRLAGEALLEHVIGTVEPPDVTELDVNLVGEYNIAGELWDVLPVLAKMGIRVRACISGDARYADVAAAHRARA
TMVVCSRALLGLARGLEDRYGIPWFEGSFYGVRAMNDTLREFARLLGGAELARRAEEVIALEQTAVDLALEPYRERLAGR
RAVLYTGGVKSWSIVSALQDLGIEVVANGITKSSDGDVEKIRELLGPDARIVSEGSPRELLRIAEETRADILVAGGRNQY
TALKGRLPFLDINQERHIPYAGYRGAVELARRLDMALSNPVWEQVRAPAPWDVEGVA

Sequences:

>Translated_457_residues
MAATDRATLFTEPACDHNREKSAKERKAGCPKPAPGGTSGGCTFDGAMITLVPIVDSAHVVHGPIACAGNSWDGRGSLSS
GPDLYRRGFTSDVGEQDVIFGGEQRLFDTILEAVRRHHPPAVFVYSTCVTAMIGDDLDAVCSAAAEHTGVPVIPVHAPGF
AGNKNLGNRLAGEALLEHVIGTVEPPDVTELDVNLVGEYNIAGELWDVLPVLAKMGIRVRACISGDARYADVAAAHRARA
TMVVCSRALLGLARGLEDRYGIPWFEGSFYGVRAMNDTLREFARLLGGAELARRAEEVIALEQTAVDLALEPYRERLAGR
RAVLYTGGVKSWSIVSALQDLGIEVVANGITKSSDGDVEKIRELLGPDARIVSEGSPRELLRIAEETRADILVAGGRNQY
TALKGRLPFLDINQERHIPYAGYRGAVELARRLDMALSNPVWEQVRAPAPWDVEGVA
>Mature_456_residues
AATDRATLFTEPACDHNREKSAKERKAGCPKPAPGGTSGGCTFDGAMITLVPIVDSAHVVHGPIACAGNSWDGRGSLSSG
PDLYRRGFTSDVGEQDVIFGGEQRLFDTILEAVRRHHPPAVFVYSTCVTAMIGDDLDAVCSAAAEHTGVPVIPVHAPGFA
GNKNLGNRLAGEALLEHVIGTVEPPDVTELDVNLVGEYNIAGELWDVLPVLAKMGIRVRACISGDARYADVAAAHRARAT
MVVCSRALLGLARGLEDRYGIPWFEGSFYGVRAMNDTLREFARLLGGAELARRAEEVIALEQTAVDLALEPYRERLAGRR
AVLYTGGVKSWSIVSALQDLGIEVVANGITKSSDGDVEKIRELLGPDARIVSEGSPRELLRIAEETRADILVAGGRNQYT
ALKGRLPFLDINQERHIPYAGYRGAVELARRLDMALSNPVWEQVRAPAPWDVEGVA

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 49079; Mature: 48948

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATDRATLFTEPACDHNREKSAKERKAGCPKPAPGGTSGGCTFDGAMITLVPIVDSAHV
CCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCEEEEEEECCCCEE
VHGPIACAGNSWDGRGSLSSGPDLYRRGFTSDVGEQDVIFGGEQRLFDTILEAVRRHHPP
EECCEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCC
AVFVYSTCVTAMIGDDLDAVCSAAAEHTGVPVIPVHAPGFAGNKNLGNRLAGEALLEHVI
EEEHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHH
GTVEPPDVTELDVNLVGEYNIAGELWDVLPVLAKMGIRVRACISGDARYADVAAAHRARA
CCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHH
TMVVCSRALLGLARGLEDRYGIPWFEGSFYGVRAMNDTLREFARLLGGAELARRAEEVIA
HHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LEQTAVDLALEPYRERLAGRRAVLYTGGVKSWSIVSALQDLGIEVVANGITKSSDGDVEK
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCHHEECCCCCCCCCCCHHH
IRELLGPDARIVSEGSPRELLRIAEETRADILVAGGRNQYTALKGRLPFLDINQERHIPY
HHHHHCCCCEEECCCCHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCEEECCCCCCCCC
AGYRGAVELARRLDMALSNPVWEQVRAPAPWDVEGVA
CCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AATDRATLFTEPACDHNREKSAKERKAGCPKPAPGGTSGGCTFDGAMITLVPIVDSAHV
CCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCEEEEEEECCCCEE
VHGPIACAGNSWDGRGSLSSGPDLYRRGFTSDVGEQDVIFGGEQRLFDTILEAVRRHHPP
EECCEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCC
AVFVYSTCVTAMIGDDLDAVCSAAAEHTGVPVIPVHAPGFAGNKNLGNRLAGEALLEHVI
EEEHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHH
GTVEPPDVTELDVNLVGEYNIAGELWDVLPVLAKMGIRVRACISGDARYADVAAAHRARA
CCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHH
TMVVCSRALLGLARGLEDRYGIPWFEGSFYGVRAMNDTLREFARLLGGAELARRAEEVIA
HHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LEQTAVDLALEPYRERLAGRRAVLYTGGVKSWSIVSALQDLGIEVVANGITKSSDGDVEK
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCHHEECCCCCCCCCCCHHH
IRELLGPDARIVSEGSPRELLRIAEETRADILVAGGRNQYTALKGRLPFLDINQERHIPY
HHHHHCCCCEEECCCCHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCEEECCCCCCCCC
AGYRGAVELARRLDMALSNPVWEQVRAPAPWDVEGVA
CCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10217509 [H]