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The map label for this gene is walK [H]

Identifier: 158318232

GI number: 158318232

Start: 7898351

End: 7900189

Strand: Direct

Name: walK [H]

Synonym: Franean1_6497

Alternate gene names: 158318232

Gene position: 7898351-7900189 (Clockwise)

Preceding gene: 158318231

Following gene: 158318233

Centisome position: 87.93

GC content: 75.8

Gene sequence:

>1839_bases
GTGACGATGACCGGCACCACCGGCACCCACCGGCGGCGCGGCCCGGCCGGCTGGTCGCTGCGTACCAGGCTGCTCGCGCT
GCTCCTCGCGCTGCTCGCCGTGATGTCCACGGTGATCGTCTTCGTGACGGCAGTCGCGTTGCGCGGGGTGCTCGTCCAGC
AGCTCGACGACCGGTTGGAGGACGCGAGCCGCCGCTGGGGGCTGACCAGCCAGGCCGGACCGGTCATGCTGCCCGGCTAT
GGAAACGCCGGCCCGGGGGGTGCCGGAACTCCCAGCCTGGCCCAGCCGCCCACGCCGGACCTGACCGCCGACGAGCGGGT
GGACTGGTTCCTGGGCTTCCGCGGTCAGCCGTCGGGGACCCTCGGCGCGGTCTTCAAGCTGCGGGATCCCGACACCACCG
CCCAGAGCTCGGCGCGCGAGGCGCTGCGGGGCGCGCCACCGCCGGGATCGGCGGCCCCGGACCTGACCTCCCTGATCGAG
AGCGCCGCCGTCGCCGTCATCGACGACAACGGCGACGTGCAGCCGCTCACCGGCGTGGACGCCGCCGTGGTGGCCCAGAT
CCCGCTCGACGGCCAGGAACACACCCGCGCGATCGCCGACCAGGGCGACTACCGCCTCATCGCCCACTTCGACCCCAACG
GGCAGACGGTGCTCGTCACCGGCGTGCCGATGAACGAGGTCAGCGCGACCCTGACCGAGGTCGGGATCGCCGGAGCGATC
GTGGCGGCGCTGGGGGTGCTGGCCGCGGGCATCGCGGGCGCGGCGATCATCCGGGTCACGCTGCGGCCGCTGAACCGGGT
CGCGGCGACGGCGAGCCGGGTCGCCGAGCTCCCGCTCGACCGGGGGGCCGTCGACCTCATGGCGGTGGCACCGGACGTCG
ACACCGACCCCCGGACCGAGGTCGGGCAGGTCGGCGCGGCGCTGAACCGGATGCTCGGCCACATCGGGGCGGCGTTCTCG
GCCCGGCACGCGAGCGAGACGCGGATGCGCCAGTTCCTCGCCGACGCCAGCCACGAGCTGCGCACACCGCTGGCGGCGAT
CAGCGGCTACGCCGAGCTCACCCGGCGCACCCGCGACACCGTCCCGCCGGACGTCGCCTACGCGATGGGCCGGGTCGAGT
CGGAGAGCGCGCGGATGACCGCGCTCGTCTCCGACCTGCTGCTGCTGGCCCGCCTCGACTCCGGACGCCCGCTCGTGAGG
GAGACCGTCGACCTCTCCCGGCTCGTGGTGGACGCGGTCAGCGACGCCCACGTGGCCGCGCCCGAGCACCGCTTCGAGCT
GGACCTGCCCAGCATGCCCGTGACCGTGGCGGGCGATCCGGCCCGGCTGCACCAGGTCCTCGCCAACCTGCTCGCCAACG
CCCGCACGCACACCCCGCCGGGCAGCCGGGTGACGGCCCGGCTGACCGTCGAGCCGGGACCTTCCGCTCCGGGGCCCTCC
GCTCCGGTGGCGAACGAGCCTGGCGCGGGCCGGCCGTCCGCGGTGCTCTCGGTGATCGACGACGGGCCGGGCATCCCGGA
CGCACTGCTTCCGAAGGTGTTCGAGCGCTTCGCGCGGGGGGACAGTTCGCGTTCCCGCGCGGCCGGCAGCACCGGCCTCG
GGCTGTCCATCGTGGCGGCGGTGGTCGAGGCGCACCATGGACGGGTCTCGGCCGCGAGCCGTCCCGGCCGCACCGCCTTC
ACCGTGGTCCTGCCCCTGGACACCGCCGACCCGGGCGCCCCGGACCCGGGCGCCCTCGACCCACCGGGCGCCCTCGACCC
ACCGGGCGCCGTCGACGGCTGCGCGCCCGACGCCGCCGATCCCGCCGTGACGGCCGCGGGGACCCGCCCTCACAGGTGA

Upstream 100 bases:

>100_bases
TCTCCTACCTGCGCAAGAAGATCGACGCCGGCCGGCCCGCGATGATCCACACAATGCGCGGCGCCGGCTACGTGCTCCGG
CCGGCGACCTGACCGGGCCC

Downstream 100 bases:

>100_bases
TGCACCGGTGGGGCACTGTCCCACCCAGAGGTCGGGTAGTCGCCCTCGTTCGGTGAGCAGTTCCTCGGCCTGACGGCGCT
AGGCTGATGTGGCGGGATCA

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MTMTGTTGTHRRRGPAGWSLRTRLLALLLALLAVMSTVIVFVTAVALRGVLVQQLDDRLEDASRRWGLTSQAGPVMLPGY
GNAGPGGAGTPSLAQPPTPDLTADERVDWFLGFRGQPSGTLGAVFKLRDPDTTAQSSAREALRGAPPPGSAAPDLTSLIE
SAAVAVIDDNGDVQPLTGVDAAVVAQIPLDGQEHTRAIADQGDYRLIAHFDPNGQTVLVTGVPMNEVSATLTEVGIAGAI
VAALGVLAAGIAGAAIIRVTLRPLNRVAATASRVAELPLDRGAVDLMAVAPDVDTDPRTEVGQVGAALNRMLGHIGAAFS
ARHASETRMRQFLADASHELRTPLAAISGYAELTRRTRDTVPPDVAYAMGRVESESARMTALVSDLLLLARLDSGRPLVR
ETVDLSRLVVDAVSDAHVAAPEHRFELDLPSMPVTVAGDPARLHQVLANLLANARTHTPPGSRVTARLTVEPGPSAPGPS
APVANEPGAGRPSAVLSVIDDGPGIPDALLPKVFERFARGDSSRSRAAGSTGLGLSIVAAVVEAHHGRVSAASRPGRTAF
TVVLPLDTADPGAPDPGALDPPGALDPPGAVDGCAPDAADPAVTAAGTRPHR

Sequences:

>Translated_612_residues
MTMTGTTGTHRRRGPAGWSLRTRLLALLLALLAVMSTVIVFVTAVALRGVLVQQLDDRLEDASRRWGLTSQAGPVMLPGY
GNAGPGGAGTPSLAQPPTPDLTADERVDWFLGFRGQPSGTLGAVFKLRDPDTTAQSSAREALRGAPPPGSAAPDLTSLIE
SAAVAVIDDNGDVQPLTGVDAAVVAQIPLDGQEHTRAIADQGDYRLIAHFDPNGQTVLVTGVPMNEVSATLTEVGIAGAI
VAALGVLAAGIAGAAIIRVTLRPLNRVAATASRVAELPLDRGAVDLMAVAPDVDTDPRTEVGQVGAALNRMLGHIGAAFS
ARHASETRMRQFLADASHELRTPLAAISGYAELTRRTRDTVPPDVAYAMGRVESESARMTALVSDLLLLARLDSGRPLVR
ETVDLSRLVVDAVSDAHVAAPEHRFELDLPSMPVTVAGDPARLHQVLANLLANARTHTPPGSRVTARLTVEPGPSAPGPS
APVANEPGAGRPSAVLSVIDDGPGIPDALLPKVFERFARGDSSRSRAAGSTGLGLSIVAAVVEAHHGRVSAASRPGRTAF
TVVLPLDTADPGAPDPGALDPPGALDPPGAVDGCAPDAADPAVTAAGTRPHR
>Mature_611_residues
TMTGTTGTHRRRGPAGWSLRTRLLALLLALLAVMSTVIVFVTAVALRGVLVQQLDDRLEDASRRWGLTSQAGPVMLPGYG
NAGPGGAGTPSLAQPPTPDLTADERVDWFLGFRGQPSGTLGAVFKLRDPDTTAQSSAREALRGAPPPGSAAPDLTSLIES
AAVAVIDDNGDVQPLTGVDAAVVAQIPLDGQEHTRAIADQGDYRLIAHFDPNGQTVLVTGVPMNEVSATLTEVGIAGAIV
AALGVLAAGIAGAAIIRVTLRPLNRVAATASRVAELPLDRGAVDLMAVAPDVDTDPRTEVGQVGAALNRMLGHIGAAFSA
RHASETRMRQFLADASHELRTPLAAISGYAELTRRTRDTVPPDVAYAMGRVESESARMTALVSDLLLLARLDSGRPLVRE
TVDLSRLVVDAVSDAHVAAPEHRFELDLPSMPVTVAGDPARLHQVLANLLANARTHTPPGSRVTARLTVEPGPSAPGPSA
PVANEPGAGRPSAVLSVIDDGPGIPDALLPKVFERFARGDSSRSRAAGSTGLGLSIVAAVVEAHHGRVSAASRPGRTAFT
VVLPLDTADPGAPDPGALDPPGALDPPGAVDGCAPDAADPAVTAAGTRPHR

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=251, Percent_Identity=32.2709163346614, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788393, Length=273, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1786783, Length=330, Percent_Identity=30.9090909090909, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1790346, Length=280, Percent_Identity=28.2142857142857, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1790861, Length=288, Percent_Identity=31.9444444444444, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1789149, Length=245, Percent_Identity=30.6122448979592, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1788279, Length=342, Percent_Identity=25.1461988304094, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1790551, Length=338, Percent_Identity=27.5147928994083, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI145693157, Length=253, Percent_Identity=30.4347826086957, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1786912, Length=256, Percent_Identity=31.640625, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788713, Length=265, Percent_Identity=25.6603773584906, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1790300, Length=248, Percent_Identity=28.6290322580645, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1787894, Length=276, Percent_Identity=27.536231884058, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789403, Length=351, Percent_Identity=28.2051282051282, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1790436, Length=241, Percent_Identity=29.8755186721992, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI48994928, Length=241, Percent_Identity=26.1410788381743, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082128, Length=242, Percent_Identity=26.0330578512397, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 62943; Mature: 62812

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMTGTTGTHRRRGPAGWSLRTRLLALLLALLAVMSTVIVFVTAVALRGVLVQQLDDRLE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DASRRWGLTSQAGPVMLPGYGNAGPGGAGTPSLAQPPTPDLTADERVDWFLGFRGQPSGT
HHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
LGAVFKLRDPDTTAQSSAREALRGAPPPGSAAPDLTSLIESAAVAVIDDNGDVQPLTGVD
EEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCEEEEECCCCCCCCCCCCC
AAVVAQIPLDGQEHTRAIADQGDYRLIAHFDPNGQTVLVTGVPMNEVSATLTEVGIAGAI
EEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHH
VAALGVLAAGIAGAAIIRVTLRPLNRVAATASRVAELPLDRGAVDLMAVAPDVDTDPRTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHH
VGQVGAALNRMLGHIGAAFSARHASETRMRQFLADASHELRTPLAAISGYAELTRRTRDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VPPDVAYAMGRVESESARMTALVSDLLLLARLDSGRPLVRETVDLSRLVVDAVSDAHVAA
CCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHCC
PEHRFELDLPSMPVTVAGDPARLHQVLANLLANARTHTPPGSRVTARLTVEPGPSAPGPS
CCCCEEECCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCC
APVANEPGAGRPSAVLSVIDDGPGIPDALLPKVFERFARGDSSRSRAAGSTGLGLSIVAA
CCCCCCCCCCCHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHH
VVEAHHGRVSAASRPGRTAFTVVLPLDTADPGAPDPGALDPPGALDPPGAVDGCAPDAAD
HHHHHCCCEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAVTAAGTRPHR
CCEEECCCCCCC
>Mature Secondary Structure 
TMTGTTGTHRRRGPAGWSLRTRLLALLLALLAVMSTVIVFVTAVALRGVLVQQLDDRLE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DASRRWGLTSQAGPVMLPGYGNAGPGGAGTPSLAQPPTPDLTADERVDWFLGFRGQPSGT
HHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
LGAVFKLRDPDTTAQSSAREALRGAPPPGSAAPDLTSLIESAAVAVIDDNGDVQPLTGVD
EEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCEEEEECCCCCCCCCCCCC
AAVVAQIPLDGQEHTRAIADQGDYRLIAHFDPNGQTVLVTGVPMNEVSATLTEVGIAGAI
EEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHH
VAALGVLAAGIAGAAIIRVTLRPLNRVAATASRVAELPLDRGAVDLMAVAPDVDTDPRTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHH
VGQVGAALNRMLGHIGAAFSARHASETRMRQFLADASHELRTPLAAISGYAELTRRTRDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VPPDVAYAMGRVESESARMTALVSDLLLLARLDSGRPLVRETVDLSRLVVDAVSDAHVAA
CCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHCC
PEHRFELDLPSMPVTVAGDPARLHQVLANLLANARTHTPPGSRVTARLTVEPGPSAPGPS
CCCCEEECCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCC
APVANEPGAGRPSAVLSVIDDGPGIPDALLPKVFERFARGDSSRSRAAGSTGLGLSIVAA
CCCCCCCCCCCHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHH
VVEAHHGRVSAASRPGRTAFTVVLPLDTADPGAPDPGALDPPGALDPPGAVDGCAPDAAD
HHHHHCCCEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAVTAAGTRPHR
CCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA