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The map label for this gene is galE [C]

Identifier: 158317879

GI number: 158317879

Start: 7466903

End: 7467943

Strand: Reverse

Name: galE [C]

Synonym: Franean1_6137

Alternate gene names: 158317879

Gene position: 7467943-7466903 (Counterclockwise)

Preceding gene: 158317880

Following gene: 158317878

Centisome position: 83.14

GC content: 69.84

Gene sequence:

>1041_bases
ATGAGGCCACGTCGCGTGCTTGTGACAGGGGTGTCGCGTCCGCTGGGCGCCGAGGTCGCTGCCGCACTCGCCGCCGACCC
CGAGATCGTCGATGTGGTCGGGGTCGACACCATCGCCCCGACGGCGGATCTCGGCCGGACCCAGTTCGTCCGGGTGGACA
TCCGAAACCCGCTGATCGCGAAGGTCATCTCCACCGCCGCCATCGACACCGTCCTGCATCTGAGTGTGCTCGCCACGCCG
CTCGGCGCCGGCGGGCGCACGGCGATGAAGGAGATCAACGTCATCGGGACGATGCAGCTCCTCGCCGCCTGCCAGAAGAC
CCCCGGGGTGAAGAAACTGGTGGTGAAGTCGACGACGTCGATCTACGGCTCGTCGCCGCGCGACCCCGCGCTGTTCACCG
AGGAGATGGAACCGCGCGGCCTTCCCGGCGGCGGTTACGCCAAGGATGCCGTCGAGGTCGAGGGGTACGTCCGAGGCTTC
GGCCGGAGGCGCCCCGACATCGCGGTGACGGTGCTGCGCCTGGCGAACGTGCTCGGCCCGCGAGTGGACAGCCCGCTCGC
GCGGTACCTCGACCTTCCGCTGGTCCCGACGGTCCTCGGCTTCGACCCACGGATTCAGCTGCTGCACTCCGACGACGCGA
TGGCGGTACTCCTGAAGGCCACCCGGGAGACCCACGCCGGCACCTTCAACGTGGCCGGGGACGGCGTGCTCCTGCTCTCG
CAGGCGATCCGGCGGGCCGGGCGGCCGGCGCTGCCAGTGCCCTTCCCCGCGATCGGGTCGTTGGGCAACATCGCCCGCCG
GCTGCGGCTCGTCGACTTCTCCTCCGAGCAGCTCGGGTTCCTCGCGCACGGGCGCGCGGTGGACACCACCAAGCTCAAAG
AGGTGTTCGGATACGTTCCTCAGTACACGACCGTCGCGACGTTCGACAGTTTCGTTCAGGACCGCGGACTGCGGTTCACC
ATCGACCACGAGCTGGTCTCGCGGGTGGAGCACGGCCTTCAGGGCGCGCTCGCCCGACGCCGGCTGCTCGGCACGTCCTG
A

Upstream 100 bases:

>100_bases
CGATGTGACGCTACGCAGCGATGAGGGTGGTGCGCGGCGTACGATGACCCCGCGAGGCCGGCCGCTGGCCGAGTCGGTAG
GTCATGCCGGGGAGGCGACC

Downstream 100 bases:

>100_bases
TGGGCGAGCGGGACACCAGCTCGCGGAGAGGGGCCGACGTGCCGATGCCACCGGATGGACGCGCCGGTCCCCCGGGGGCG
GCCGGTCCGCCGCCGCCGCG

Product: NAD-dependent epimerase/dehydratase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MRPRRVLVTGVSRPLGAEVAAALAADPEIVDVVGVDTIAPTADLGRTQFVRVDIRNPLIAKVISTAAIDTVLHLSVLATP
LGAGGRTAMKEINVIGTMQLLAACQKTPGVKKLVVKSTTSIYGSSPRDPALFTEEMEPRGLPGGGYAKDAVEVEGYVRGF
GRRRPDIAVTVLRLANVLGPRVDSPLARYLDLPLVPTVLGFDPRIQLLHSDDAMAVLLKATRETHAGTFNVAGDGVLLLS
QAIRRAGRPALPVPFPAIGSLGNIARRLRLVDFSSEQLGFLAHGRAVDTTKLKEVFGYVPQYTTVATFDSFVQDRGLRFT
IDHELVSRVEHGLQGALARRRLLGTS

Sequences:

>Translated_346_residues
MRPRRVLVTGVSRPLGAEVAAALAADPEIVDVVGVDTIAPTADLGRTQFVRVDIRNPLIAKVISTAAIDTVLHLSVLATP
LGAGGRTAMKEINVIGTMQLLAACQKTPGVKKLVVKSTTSIYGSSPRDPALFTEEMEPRGLPGGGYAKDAVEVEGYVRGF
GRRRPDIAVTVLRLANVLGPRVDSPLARYLDLPLVPTVLGFDPRIQLLHSDDAMAVLLKATRETHAGTFNVAGDGVLLLS
QAIRRAGRPALPVPFPAIGSLGNIARRLRLVDFSSEQLGFLAHGRAVDTTKLKEVFGYVPQYTTVATFDSFVQDRGLRFT
IDHELVSRVEHGLQGALARRRLLGTS
>Mature_346_residues
MRPRRVLVTGVSRPLGAEVAAALAADPEIVDVVGVDTIAPTADLGRTQFVRVDIRNPLIAKVISTAAIDTVLHLSVLATP
LGAGGRTAMKEINVIGTMQLLAACQKTPGVKKLVVKSTTSIYGSSPRDPALFTEEMEPRGLPGGGYAKDAVEVEGYVRGF
GRRRPDIAVTVLRLANVLGPRVDSPLARYLDLPLVPTVLGFDPRIQLLHSDDAMAVLLKATRETHAGTFNVAGDGVLLLS
QAIRRAGRPALPVPFPAIGSLGNIARRLRLVDFSSEQLGFLAHGRAVDTTKLKEVFGYVPQYTTVATFDSFVQDRGLRFT
IDHELVSRVEHGLQGALARRRLLGTS

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786974, Length=191, Percent_Identity=30.3664921465969, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 37060; Mature: 37060

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRRVLVTGVSRPLGAEVAAALAADPEIVDVVGVDTIAPTADLGRTQFVRVDIRNPLIA
CCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCHHHH
KVISTAAIDTVLHLSVLATPLGAGGRTAMKEINVIGTMQLLAACQKTPGVKKLVVKSTTS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IYGSSPRDPALFTEEMEPRGLPGGGYAKDAVEVEGYVRGFGRRRPDIAVTVLRLANVLGP
HCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
RVDSPLARYLDLPLVPTVLGFDPRIQLLHSDDAMAVLLKATRETHAGTFNVAGDGVLLLS
CCCCHHHHHHCCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCHHHHHH
QAIRRAGRPALPVPFPAIGSLGNIARRLRLVDFSSEQLGFLAHGRAVDTTKLKEVFGYVP
HHHHHCCCCCCCCCCHHHCCHHHHHHHHHHEECCCCCCCCEECCCCCCHHHHHHHHCCCC
QYTTVATFDSFVQDRGLRFTIDHELVSRVEHGLQGALARRRLLGTS
CCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRPRRVLVTGVSRPLGAEVAAALAADPEIVDVVGVDTIAPTADLGRTQFVRVDIRNPLIA
CCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCHHHH
KVISTAAIDTVLHLSVLATPLGAGGRTAMKEINVIGTMQLLAACQKTPGVKKLVVKSTTS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IYGSSPRDPALFTEEMEPRGLPGGGYAKDAVEVEGYVRGFGRRRPDIAVTVLRLANVLGP
HCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
RVDSPLARYLDLPLVPTVLGFDPRIQLLHSDDAMAVLLKATRETHAGTFNVAGDGVLLLS
CCCCHHHHHHCCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCHHHHHH
QAIRRAGRPALPVPFPAIGSLGNIARRLRLVDFSSEQLGFLAHGRAVDTTKLKEVFGYVP
HHHHHCCCCCCCCCCHHHCCHHHHHHHHHHEECCCCCCCCEECCCCCCHHHHHHHHCCCC
QYTTVATFDSFVQDRGLRFTIDHELVSRVEHGLQGALARRRLLGTS
CCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]