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The map label for this gene is guaA

Identifier: 158317731

GI number: 158317731

Start: 7300224

End: 7301828

Strand: Reverse

Name: guaA

Synonym: Franean1_5989

Alternate gene names: 158317731

Gene position: 7301828-7300224 (Counterclockwise)

Preceding gene: 158317736

Following gene: 158317730

Centisome position: 81.29

GC content: 70.72

Gene sequence:

>1605_bases
GTGAACGAGTCACCTCGCGCCAGCTATGGCTCCACCGGCGCACATCCCGGTTACGATCCGGTTCTCGTGATCGACTTCGG
CGCCCAGTACGCCCAGTTGATCGCTCGGCGCGTTCGCGAGTGCCATGTGTACTCGGAGATCGTCCCGTGGGATATGCCGG
TAGCCGAGATCCTGGCCCGGCGCCCGGCCGCGCTCATCCTCTCTGGCGGGCCGAAGTCCGTCTACTCCCCGGGTGCCCCC
CGGGTCGACCCCGCGCTGTTCGCTGCCGGGGTGCCCGTCCTGGGCATCTGCTACGGCCACCAGGTGATGGCGCAGGCGCT
GGACGGCACGGTCGCCCGCACCGGCACCGCCGAGTACGGAGCCACCCGGCTGCGGGTGGACGACCCCGGGGTGCTCTTCG
ACGGCCTCCCCACCAGCCAGCAGGTCTGGATGTCGCACGGCGACTCGGTGACCGCCGCGCCCCCCGGTTTCCGGGTGACC
GCGTCGACGCCGTCCACCCCGGTCGCGGCGTTCGAGGACCCCACCCGCAGGCTCTACGGCGTGCAGTTCCACCCCGAGGT
CGTGCACAGCGAGCGCGGGATGGATGTCCTGCGGCACTTCCTGCTGGTCGGCGCGGGCTGCCGGCCGTCCTGGACGATGA
TCAATATCGTCGAGGAGGCCGTGACCGCGGTGCGGGCGCAGGTCGGCAATGGCCGGCTGATCTGCGGCCTGTCCGGCGGG
GTCGATTCGGCGGTGGCCGCCGCGCTGGTACAGCGAGCCGTCGGTGACACCCTGACCTGCGTGTTCGTCGACCACGGGCT
GTTGCGGGCGGGCGAGGCGGAACAGGTCGAACGCGACTTCGTCGCCTCCACCGGCGTCGACCTCGTCCACGTGAAGGCGG
CGGACCGCTTCGCCTCCGCGCTGGCCGGGGTGACGGATCCGGAGCAGAAGCGGAAGATCATCGGGCGGGAGTTCATCCGC
GTCTTCGAGGAGTCCGCGCGTGAGCTCGACGCCCGCGCGGAGGCGGAGGGAACGCACATCGGTTTCCTCGTCCAGGGCAC
TCTCTACCCGGACGTCATCGAGTCCGGCTCGCCCACCGCCGCCAAGATCAAGTCCCATCACAATGTCGGCGGGCTGCCGG
ACGACCTGCAGTTCGACCTGGTCGAGCCGCTGCGCACCCTGTTCAAGGACGAGGTCCGCCGGCTCGGTGAGGAGCTCGGC
CTGCCCGAGGACATCGTCTGGCGCCAGCCGTTCCCGGGCCCGGGGCTGGCCGTCCGCATCATCGGTGAGGTCACCCCCGA
GCGGCTCGAGATCGTCCGGGCCGCCGACGCGGTCGTCCGGGACGAGATCCGCCGAGCGGGGCTCGACCGGGAGATCTGGC
AGGTCTTCGCCGTGCTGCTGGCGGACGTCCGGTCGGTCGGCGTCCAGGGCGACGAGCGGACCTACGGTTTCCCGGTGGTG
CTGCGCGCGGTGACCAGCGAGGACGCGATGACCGCCGACTGGGCTCGGCTGCCCTACGACCTGCTGGAACGCATCAGCAA
CCGGGTCGTCAACGAGGTTGGGCAGGTCAACCGGGTCGTCTACGACATCACCTCGAAGCCGCCGGGCACGATCGAGTGGG
AGTAG

Upstream 100 bases:

>100_bases
CAGCGGTTCGCCGCGACGGCCGAAGGTGGCCCCAATGTGGGGCGAGTGCACGGCCATGCCGCCTGATGCTCGGGTCCACC
GTCCTCCGTAGGCTTGATGG

Downstream 100 bases:

>100_bases
CCCCCACAGGTGAGGCCGGCGCCGCGGCCGCCGTGCCGTGCTACGGGCCGTCCGCGCTACGGGGCGGGCGGCCCGGCCGG
CGTCCGTCGTCGGCCGGGCC

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MNESPRASYGSTGAHPGYDPVLVIDFGAQYAQLIARRVRECHVYSEIVPWDMPVAEILARRPAALILSGGPKSVYSPGAP
RVDPALFAAGVPVLGICYGHQVMAQALDGTVARTGTAEYGATRLRVDDPGVLFDGLPTSQQVWMSHGDSVTAAPPGFRVT
ASTPSTPVAAFEDPTRRLYGVQFHPEVVHSERGMDVLRHFLLVGAGCRPSWTMINIVEEAVTAVRAQVGNGRLICGLSGG
VDSAVAAALVQRAVGDTLTCVFVDHGLLRAGEAEQVERDFVASTGVDLVHVKAADRFASALAGVTDPEQKRKIIGREFIR
VFEESARELDARAEAEGTHIGFLVQGTLYPDVIESGSPTAAKIKSHHNVGGLPDDLQFDLVEPLRTLFKDEVRRLGEELG
LPEDIVWRQPFPGPGLAVRIIGEVTPERLEIVRAADAVVRDEIRRAGLDREIWQVFAVLLADVRSVGVQGDERTYGFPVV
LRAVTSEDAMTADWARLPYDLLERISNRVVNEVGQVNRVVYDITSKPPGTIEWE

Sequences:

>Translated_534_residues
MNESPRASYGSTGAHPGYDPVLVIDFGAQYAQLIARRVRECHVYSEIVPWDMPVAEILARRPAALILSGGPKSVYSPGAP
RVDPALFAAGVPVLGICYGHQVMAQALDGTVARTGTAEYGATRLRVDDPGVLFDGLPTSQQVWMSHGDSVTAAPPGFRVT
ASTPSTPVAAFEDPTRRLYGVQFHPEVVHSERGMDVLRHFLLVGAGCRPSWTMINIVEEAVTAVRAQVGNGRLICGLSGG
VDSAVAAALVQRAVGDTLTCVFVDHGLLRAGEAEQVERDFVASTGVDLVHVKAADRFASALAGVTDPEQKRKIIGREFIR
VFEESARELDARAEAEGTHIGFLVQGTLYPDVIESGSPTAAKIKSHHNVGGLPDDLQFDLVEPLRTLFKDEVRRLGEELG
LPEDIVWRQPFPGPGLAVRIIGEVTPERLEIVRAADAVVRDEIRRAGLDREIWQVFAVLLADVRSVGVQGDERTYGFPVV
LRAVTSEDAMTADWARLPYDLLERISNRVVNEVGQVNRVVYDITSKPPGTIEWE
>Mature_534_residues
MNESPRASYGSTGAHPGYDPVLVIDFGAQYAQLIARRVRECHVYSEIVPWDMPVAEILARRPAALILSGGPKSVYSPGAP
RVDPALFAAGVPVLGICYGHQVMAQALDGTVARTGTAEYGATRLRVDDPGVLFDGLPTSQQVWMSHGDSVTAAPPGFRVT
ASTPSTPVAAFEDPTRRLYGVQFHPEVVHSERGMDVLRHFLLVGAGCRPSWTMINIVEEAVTAVRAQVGNGRLICGLSGG
VDSAVAAALVQRAVGDTLTCVFVDHGLLRAGEAEQVERDFVASTGVDLVHVKAADRFASALAGVTDPEQKRKIIGREFIR
VFEESARELDARAEAEGTHIGFLVQGTLYPDVIESGSPTAAKIKSHHNVGGLPDDLQFDLVEPLRTLFKDEVRRLGEELG
LPEDIVWRQPFPGPGLAVRIIGEVTPERLEIVRAADAVVRDEIRRAGLDREIWQVFAVLLADVRSVGVQGDERTYGFPVV
LRAVTSEDAMTADWARLPYDLLERISNRVVNEVGQVNRVVYDITSKPPGTIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=560, Percent_Identity=36.9642857142857, Blast_Score=318, Evalue=6e-87,
Organism=Escherichia coli, GI1788854, Length=525, Percent_Identity=51.6190476190476, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI1789760, Length=152, Percent_Identity=32.8947368421053, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71992717, Length=574, Percent_Identity=36.7595818815331, Blast_Score=318, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI71992710, Length=574, Percent_Identity=36.7595818815331, Blast_Score=317, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI133901714, Length=574, Percent_Identity=36.7595818815331, Blast_Score=317, Evalue=8e-87,
Organism=Saccharomyces cerevisiae, GI6323873, Length=521, Percent_Identity=48.5604606525912, Blast_Score=506, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6322638, Length=164, Percent_Identity=31.0975609756098, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281365319, Length=553, Percent_Identity=35.9855334538879, Blast_Score=302, Evalue=5e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_FRASN (A8LAX2)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001510239.1
- ProteinModelPortal:   A8LAX2
- GeneID:   5674310
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_5989
- HOGENOM:   HBG391775
- OMA:   MKLVEPL
- ProtClustDB:   PRK00074
- BioCyc:   FSP1855:FRANEAN1_5989-MONOMER
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 57898; Mature: 57898

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 97-97 ACT_SITE 184-184 ACT_SITE 186-186

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNESPRASYGSTGAHPGYDPVLVIDFGAQYAQLIARRVRECHVYSEIVPWDMPVAEILAR
CCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
RPAALILSGGPKSVYSPGAPRVDPALFAAGVPVLGICYGHQVMAQALDGTVARTGTAEYG
CCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHC
ATRLRVDDPGVLFDGLPTSQQVWMSHGDSVTAAPPGFRVTASTPSTPVAAFEDPTRRLYG
CEEEEECCCCCEECCCCCCHHHHHHCCCEEEECCCCCEEECCCCCCCCCCCCCCHHHHEE
VQFHPEVVHSERGMDVLRHFLLVGAGCRPSWTMINIVEEAVTAVRAQVGNGRLICGLSGG
CEECHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
VDSAVAAALVQRAVGDTLTCVFVDHGLLRAGEAEQVERDFVASTGVDLVHVKAADRFASA
HHHHHHHHHHHHHHCCCEEEEEECCCHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHH
LAGVTDPEQKRKIIGREFIRVFEESARELDARAEAEGTHIGFLVQGTLYPDVIESGSPTA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCCCH
AKIKSHHNVGGLPDDLQFDLVEPLRTLFKDEVRRLGEELGLPEDIVWRQPFPGPGLAVRI
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCEEEEE
IGEVTPERLEIVRAADAVVRDEIRRAGLDREIWQVFAVLLADVRSVGVQGDERTYGFPVV
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
LRAVTSEDAMTADWARLPYDLLERISNRVVNEVGQVNRVVYDITSKPPGTIEWE
EEEHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MNESPRASYGSTGAHPGYDPVLVIDFGAQYAQLIARRVRECHVYSEIVPWDMPVAEILAR
CCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
RPAALILSGGPKSVYSPGAPRVDPALFAAGVPVLGICYGHQVMAQALDGTVARTGTAEYG
CCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHC
ATRLRVDDPGVLFDGLPTSQQVWMSHGDSVTAAPPGFRVTASTPSTPVAAFEDPTRRLYG
CEEEEECCCCCEECCCCCCHHHHHHCCCEEEECCCCCEEECCCCCCCCCCCCCCHHHHEE
VQFHPEVVHSERGMDVLRHFLLVGAGCRPSWTMINIVEEAVTAVRAQVGNGRLICGLSGG
CEECHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
VDSAVAAALVQRAVGDTLTCVFVDHGLLRAGEAEQVERDFVASTGVDLVHVKAADRFASA
HHHHHHHHHHHHHHCCCEEEEEECCCHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHH
LAGVTDPEQKRKIIGREFIRVFEESARELDARAEAEGTHIGFLVQGTLYPDVIESGSPTA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCCCH
AKIKSHHNVGGLPDDLQFDLVEPLRTLFKDEVRRLGEELGLPEDIVWRQPFPGPGLAVRI
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCEEEEE
IGEVTPERLEIVRAADAVVRDEIRRAGLDREIWQVFAVLLADVRSVGVQGDERTYGFPVV
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
LRAVTSEDAMTADWARLPYDLLERISNRVVNEVGQVNRVVYDITSKPPGTIEWE
EEEHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA