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The map label for this gene is mdh [H]

Identifier: 158317712

GI number: 158317712

Start: 7274054

End: 7274986

Strand: Reverse

Name: mdh [H]

Synonym: Franean1_5970

Alternate gene names: 158317712

Gene position: 7274986-7274054 (Counterclockwise)

Preceding gene: 158317713

Following gene: 158317710

Centisome position: 80.99

GC content: 67.74

Gene sequence:

>933_bases
ATGCCCGGAAAAGTCACTGTCGTCGGGGCCGGTTTCTACGGTTCGACGACCGCGCAGCGACTTGCTGAGTACAACATTTT
CGACACGGTCGTCATCATCGACATCGTCGAGGGCAAGCCGCAGGGCCAGGCGCTCGACCTCAATCAGTCCCGGTCGATCG
AGGGCTTCGAGACCAAGGTCGTCGGGACCAACGACTACGCCGACACCGCCGGTTCCGACGTGGTCGTCATCACCGCCGGC
CTGCCCCGCAAGCCGGGCATGAGCCGGATGGACCTCATCGAGGTCAATGCCAAGATCGTCCGTCAGGTGTCGGAGAGCAT
CGCGAAGACGTCGCCGGACGCGGTCGTCGTTGTGGTCTCCAACCCGCTCGACGAGATGACCGCGCTCGCCGCGAACGTGC
TGGGCTTCCCGCGCTCGCGGGTGCTCGGCCAGGCGGGCATGCTCGACACCGCCCGTTTCACTGACTTCGTCGCGGAGACA
CTGAACGTGCCGGTCGCGTCGGTGAAGACCCTCACGCTGGGCTCGCACGGTGACACGATGGTCCCGGTGCCCTCGCAGTG
CACCGTCGACGGCAAGCCGTTGAGCGACCTCCTCCCGGCGGACAAGATCGAGGAGCTGGTCAACCGGACCCGCAACGGAG
GCGCGGAGGTCGTCGCGCTACTCAAGACCGGTTCGGCCTACTACGCTCCGTCCGCGGCGGCGGCCCGCATGGTCAAGGCG
GTCGCCGAGGACTCCGGCGCCGTAATGCCGGTCTGTGCCTGGGTGCAGGGCGAGTTCGGCATCTCCGACGTCTACCTCGG
CGTGCCCGCGCAGATCGGCGCCGGGGGCGTGAAGAAGGTTGTCGAGATCGACCTGACCGGCACCGAGCTCGCCGCGCTCA
AGACGGCCGCCGAGGCCGTGCGCGCCAAGCAGGCGGACGTCGCCAGCCTGTAG

Upstream 100 bases:

>100_bases
CGGTCACCTGCGACCACAACGCCGGCGCTGAAGACCCCGCCGTGGGCACCGCCGAGATCGTCGACGCGATCATCGCGATA
CTGGGGAGCTGATCAGTCCA

Downstream 100 bases:

>100_bases
CGGCGGCGACCAGCCGACAGCTGACCAGCCGATAGCTTTCGCTCCGCCGGCCCACGCACCCACAGGACGCGCTCGGACCC
CGGGAACCTCCGGGACCACC

Product: malate dehydrogenase, NAD-dependent

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MPGKVTVVGAGFYGSTTAQRLAEYNIFDTVVIIDIVEGKPQGQALDLNQSRSIEGFETKVVGTNDYADTAGSDVVVITAG
LPRKPGMSRMDLIEVNAKIVRQVSESIAKTSPDAVVVVVSNPLDEMTALAANVLGFPRSRVLGQAGMLDTARFTDFVAET
LNVPVASVKTLTLGSHGDTMVPVPSQCTVDGKPLSDLLPADKIEELVNRTRNGGAEVVALLKTGSAYYAPSAAAARMVKA
VAEDSGAVMPVCAWVQGEFGISDVYLGVPAQIGAGGVKKVVEIDLTGTELAALKTAAEAVRAKQADVASL

Sequences:

>Translated_310_residues
MPGKVTVVGAGFYGSTTAQRLAEYNIFDTVVIIDIVEGKPQGQALDLNQSRSIEGFETKVVGTNDYADTAGSDVVVITAG
LPRKPGMSRMDLIEVNAKIVRQVSESIAKTSPDAVVVVVSNPLDEMTALAANVLGFPRSRVLGQAGMLDTARFTDFVAET
LNVPVASVKTLTLGSHGDTMVPVPSQCTVDGKPLSDLLPADKIEELVNRTRNGGAEVVALLKTGSAYYAPSAAAARMVKA
VAEDSGAVMPVCAWVQGEFGISDVYLGVPAQIGAGGVKKVVEIDLTGTELAALKTAAEAVRAKQADVASL
>Mature_309_residues
PGKVTVVGAGFYGSTTAQRLAEYNIFDTVVIIDIVEGKPQGQALDLNQSRSIEGFETKVVGTNDYADTAGSDVVVITAGL
PRKPGMSRMDLIEVNAKIVRQVSESIAKTSPDAVVVVVSNPLDEMTALAANVLGFPRSRVLGQAGMLDTARFTDFVAETL
NVPVASVKTLTLGSHGDTMVPVPSQCTVDGKPLSDLLPADKIEELVNRTRNGGAEVVALLKTGSAYYAPSAAAARMVKAV
AEDSGAVMPVCAWVQGEFGISDVYLGVPAQIGAGGVKKVVEIDLTGTELAALKTAAEAVRAKQADVASL

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=312, Percent_Identity=31.7307692307692, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI221136809, Length=312, Percent_Identity=31.7307692307692, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI5031857, Length=312, Percent_Identity=33.0128205128205, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI260099723, Length=312, Percent_Identity=33.0128205128205, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI291575128, Length=316, Percent_Identity=31.9620253164557, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI4557032, Length=316, Percent_Identity=31.9620253164557, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI15082234, Length=307, Percent_Identity=30.6188925081433, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI9257228, Length=313, Percent_Identity=31.3099041533546, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI4504973, Length=313, Percent_Identity=31.3099041533546, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI260099725, Length=225, Percent_Identity=36.4444444444444, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI260099727, Length=196, Percent_Identity=37.7551020408163, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI21735621, Length=291, Percent_Identity=31.9587628865979, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI207028494, Length=198, Percent_Identity=31.8181818181818, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI103472011, Length=313, Percent_Identity=24.9201277955272, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789632, Length=251, Percent_Identity=34.2629482071713, Blast_Score=104, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17535107, Length=293, Percent_Identity=30.7167235494881, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17554310, Length=225, Percent_Identity=34.2222222222222, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6322765, Length=250, Percent_Identity=34.8, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324446, Length=166, Percent_Identity=34.3373493975904, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320125, Length=190, Percent_Identity=28.9473684210526, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17136226, Length=310, Percent_Identity=32.258064516129, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45550422, Length=313, Percent_Identity=30.0319488817891, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24647881, Length=289, Percent_Identity=33.9100346020761, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24663599, Length=308, Percent_Identity=32.7922077922078, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24663595, Length=273, Percent_Identity=30.03663003663, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 32190; Mature: 32059

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGKVTVVGAGFYGSTTAQRLAEYNIFDTVVIIDIVEGKPQGQALDLNQSRSIEGFETKV
CCCEEEEEECCCCCCHHHHHHHHCCHHEEEEEEEEECCCCCCCEECCCCCCCCCCCEEEE
VGTNDYADTAGSDVVVITAGLPRKPGMSRMDLIEVNAKIVRQVSESIAKTSPDAVVVVVS
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEEC
NPLDEMTALAANVLGFPRSRVLGQAGMLDTARFTDFVAETLNVPVASVKTLTLGSHGDTM
CCHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHEEEEEECCCCCEE
VPVPSQCTVDGKPLSDLLPADKIEELVNRTRNGGAEVVALLKTGSAYYAPSAAAARMVKA
ECCCCCCEECCCCHHHCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHH
VAEDSGAVMPVCAWVQGEFGISDVYLGVPAQIGAGGVKKVVEIDLTGTELAALKTAAEAV
HHHCCCCEEEEEEEECCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHH
RAKQADVASL
HHHHHHHCCC
>Mature Secondary Structure 
PGKVTVVGAGFYGSTTAQRLAEYNIFDTVVIIDIVEGKPQGQALDLNQSRSIEGFETKV
CCEEEEEECCCCCCHHHHHHHHCCHHEEEEEEEEECCCCCCCEECCCCCCCCCCCEEEE
VGTNDYADTAGSDVVVITAGLPRKPGMSRMDLIEVNAKIVRQVSESIAKTSPDAVVVVVS
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEEC
NPLDEMTALAANVLGFPRSRVLGQAGMLDTARFTDFVAETLNVPVASVKTLTLGSHGDTM
CCHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHEEEEEECCCCCEE
VPVPSQCTVDGKPLSDLLPADKIEELVNRTRNGGAEVVALLKTGSAYYAPSAAAARMVKA
ECCCCCCEECCCCHHHCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHH
VAEDSGAVMPVCAWVQGEFGISDVYLGVPAQIGAGGVKKVVEIDLTGTELAALKTAAEAV
HHHCCCCEEEEEEEECCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHH
RAKQADVASL
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA