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The map label for this gene is yafV [C]

Identifier: 158317480

GI number: 158317480

Start: 6966607

End: 6967446

Strand: Reverse

Name: yafV [C]

Synonym: Franean1_5732

Alternate gene names: 158317480

Gene position: 6967446-6966607 (Counterclockwise)

Preceding gene: 158317481

Following gene: 158317479

Centisome position: 77.57

GC content: 66.43

Gene sequence:

>840_bases
ATGTCCAGGACGATCCGAGCGGCGCTTGTCCAGGCGCGGTGGACGGGCGACCGAGAAACGATGATCAAGGCTCACGAGGA
GCACGCGCGGTCGGCCGCCGCGCTGGGCGCACAGGTCATCTGCTTCCAGGAGCTCTTCTACGGGCCTTATTTCTGCCAGG
TTCAGGACGCCGCCTTCTACTCCTACGCGGAGTCGGTGCCGGGGCCGATCGTCGACCGGTTCGCCGCGCTCGCCGCTGAG
CTCGGGATAGTGATGATTCTGCCGGTCTACGAGCAGGAGCAACCCGGGATCCTCTACAACACGGCGGCGGTCGTGGACGC
CGACGGCTCCTTTCTCGGCAAGTACCGCAAGACCCACATCCCGCACGTGGCCGGATTCTGGGAGAAGTTCTACTTCCGGC
CCGGGAACCTCGGATATCCGGTCTTCGACACGGCGGTCGGCCGGATCGGGGTCTACATCTGTTACGACCGTCATTTTCCG
GAGGGCTGGCGCGCGCTCGGCCTGAACGGGGCCGAGCTGGTGTTCAACCCGTCCGCCACCTCCCGCGGCCTGTCGAACTA
CCTGTGGAAGCTCGAGCAGCCCGCGGCCGCGGTCGCCAACGAGTACTACGTCGGCGCGGTGAACCGGGTGGGTGTCGAGG
ACCTGGGCGACGACGACTTCTACGGAACCTCCTACTTCGTCGACCCCGAGGGGACGTTCGTCGACGGCACCGCGGACGCG
CACGAACCGGAACTGCTGGTCCGAGACCTGGACATGAGCCTGTTGACCACGGTGCGCAACCGGTGGGCGTTCTATCGTGA
CCGACGGCCGGACACCTACGGCGACCTCACCGCGCCGTGA

Upstream 100 bases:

>100_bases
CTGACGGTTGTCGAGCTGGCCCCCAGGGCACCGGGCCAGCTCGCGGACGGTCGGCAGGCGGCCGCCTGCACCCGGCCAGC
GCGGCTGACAGGGGTGACGT

Downstream 100 bases:

>100_bases
CCGGTCGCGTGCCGTCCCGACCCGGGCCGGCAGCCAGGTGGGAAGCAGGTCCGGTGAAGGGGCAGACGACGTGAGGACGC
TGATCACCGGCGGGACGCTG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSRTIRAALVQARWTGDRETMIKAHEEHARSAAALGAQVICFQELFYGPYFCQVQDAAFYSYAESVPGPIVDRFAALAAE
LGIVMILPVYEQEQPGILYNTAAVVDADGSFLGKYRKTHIPHVAGFWEKFYFRPGNLGYPVFDTAVGRIGVYICYDRHFP
EGWRALGLNGAELVFNPSATSRGLSNYLWKLEQPAAAVANEYYVGAVNRVGVEDLGDDDFYGTSYFVDPEGTFVDGTADA
HEPELLVRDLDMSLLTTVRNRWAFYRDRRPDTYGDLTAP

Sequences:

>Translated_279_residues
MSRTIRAALVQARWTGDRETMIKAHEEHARSAAALGAQVICFQELFYGPYFCQVQDAAFYSYAESVPGPIVDRFAALAAE
LGIVMILPVYEQEQPGILYNTAAVVDADGSFLGKYRKTHIPHVAGFWEKFYFRPGNLGYPVFDTAVGRIGVYICYDRHFP
EGWRALGLNGAELVFNPSATSRGLSNYLWKLEQPAAAVANEYYVGAVNRVGVEDLGDDDFYGTSYFVDPEGTFVDGTADA
HEPELLVRDLDMSLLTTVRNRWAFYRDRRPDTYGDLTAP
>Mature_278_residues
SRTIRAALVQARWTGDRETMIKAHEEHARSAAALGAQVICFQELFYGPYFCQVQDAAFYSYAESVPGPIVDRFAALAAEL
GIVMILPVYEQEQPGILYNTAAVVDADGSFLGKYRKTHIPHVAGFWEKFYFRPGNLGYPVFDTAVGRIGVYICYDRHFPE
GWRALGLNGAELVFNPSATSRGLSNYLWKLEQPAAAVANEYYVGAVNRVGVEDLGDDDFYGTSYFVDPEGTFVDGTADAH
EPELLVRDLDMSLLTTVRNRWAFYRDRRPDTYGDLTAP

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI7706509, Length=261, Percent_Identity=39.8467432950192, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI9910460, Length=277, Percent_Identity=31.0469314079422, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI297632348, Length=283, Percent_Identity=31.095406360424, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI5031947, Length=283, Percent_Identity=31.095406360424, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI297632350, Length=283, Percent_Identity=31.095406360424, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI297632346, Length=183, Percent_Identity=32.2404371584699, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17533173, Length=262, Percent_Identity=37.7862595419847, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17556280, Length=180, Percent_Identity=30.5555555555556, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322335, Length=307, Percent_Identity=25.7328990228013, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323383, Length=295, Percent_Identity=28.135593220339, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21358471, Length=275, Percent_Identity=38.5454545454545, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI21355835, Length=286, Percent_Identity=25.8741258741259, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 31185; Mature: 31054

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTIRAALVQARWTGDRETMIKAHEEHARSAAALGAQVICFQELFYGPYFCQVQDAAFY
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHH
SYAESVPGPIVDRFAALAAELGIVMILPVYEQEQPGILYNTAAVVDADGSFLGKYRKTHI
HHHHHCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEEEEEECCCHHHHHHHHHCC
PHVAGFWEKFYFRPGNLGYPVFDTAVGRIGVYICYDRHFPEGWRALGLNGAELVFNPSAT
CHHHHHHHHHHCCCCCCCCCHHHHHHHHEEEEEEEECCCCCCHHEECCCCCEEEECCCCC
SRGLSNYLWKLEQPAAAVANEYYVGAVNRVGVEDLGDDDFYGTSYFVDPEGTFVDGTADA
CCHHHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCCCCCCCCCEEEECCCCCEECCCCCC
HEPELLVRDLDMSLLTTVRNRWAFYRDRRPDTYGDLTAP
CCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SRTIRAALVQARWTGDRETMIKAHEEHARSAAALGAQVICFQELFYGPYFCQVQDAAFY
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHH
SYAESVPGPIVDRFAALAAELGIVMILPVYEQEQPGILYNTAAVVDADGSFLGKYRKTHI
HHHHHCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEEEEEECCCHHHHHHHHHCC
PHVAGFWEKFYFRPGNLGYPVFDTAVGRIGVYICYDRHFPEGWRALGLNGAELVFNPSAT
CHHHHHHHHHHCCCCCCCCCHHHHHHHHEEEEEEEECCCCCCHHEECCCCCEEEECCCCC
SRGLSNYLWKLEQPAAAVANEYYVGAVNRVGVEDLGDDDFYGTSYFVDPEGTFVDGTADA
CCHHHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCCCCCCCCCEEEECCCCCEECCCCCC
HEPELLVRDLDMSLLTTVRNRWAFYRDRRPDTYGDLTAP
CCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]