Click here to switch to the map view.

The map label for this gene is hyuA [H]

Identifier: 158317479

GI number: 158317479

Start: 6965097

End: 6966536

Strand: Reverse

Name: hyuA [H]

Synonym: Franean1_5731

Alternate gene names: 158317479

Gene position: 6966536-6965097 (Counterclockwise)

Preceding gene: 158317480

Following gene: 158317478

Centisome position: 77.56

GC content: 70.14

Gene sequence:

>1440_bases
GTGAGGACGCTGATCACCGGCGGGACGCTGGTCGGCCCGCTGGGCGCGACGCCCACCGAGATCCTGGTCGACGGCGAGAC
CGTCGCGGCTCTGTTCGCGCCCGGCAGCCTGCCAGAAGGCCTGCGCGGCAGCCTGCACGACGGCCCCGGCTCCTCGGCCG
ACCGGGTCATCGACGCCACCGGGAAGTACGTGATCCCGGGCGGCGTCGACGTCCACACGCACATGGAGCTGCCCTTCGGC
GGCACCTCGGCGTCCGACACCTTCGCCACCGGGACGGCCGCCGCGGCCTGGGGCGGCACCACGACGATCATCGACTTCGC
GGTGCAGCGCACCGGCGAGGTGGTCGAGGAGTCGCTGGCCGCCTGGCACGCGAAGGCCGAAGGTCAGTGCGCCATCGACT
ACGGCTTCCACATGATTATCGGCGGTGTCGACGACCAGGCGCTCAAGGCGATGGACTACCTCGTCGCCAACGAGGGCATT
TCCAGCTTCAAGCTGTTCATGGCGTACCCCGGAGTGTTCTACAGCGACGACGGCCAGATCCTGCGGGCTATGCAACGGGC
GGCCGAATCCGGCGCGATGGTCATGATGCACGCGGAGAACGGCATCGCGATCGACGTCCTCGCCGCCCAGGCGCTCGCCC
GTGGCGGGACGGGCCCTGTTCACCACGGTTACACCCGTCCACCCGAGCTGGAAGGGGAGGCGACGAACCGGGCGGCGGTG
CTGGCCCGGGTCGCCGGAAACTGCCCGCTCTACTTCGTGCACATGTCGGCGTCCGAGGCGCTGGCGGTCGTTTCGGCGGC
CCGCGGCGCCGGCCACAACGTGTTCGCCGAGACCTGCCCGCAGTACCTGTATCTCAGTCTCGAGGAACATCTCGGCGCGC
CCGGCTTCGAAGGCGCGAAATACGTCGCCTCGCCACCCCTGCGGACGAACACGGCCAGCCACCACGACGATCTGTGGCGC
GGCCTGCGTACCGACGACCTCGCCGTGGTCTCCACTGACCACTGCCCGTTCTGCTTCCGGGACCAGAAGGAGCTCGGCCG
CGGCGACTTCACCAGGATCCCGAACGGGATCGGCACCGTCGAGCACCGCATCGACCTCGTCTACCAGGGAGTCGCCAGCG
GGCGTCTCTCCCTGCCCCGCTGGGTGGAAACCTGCGCGACCACCCCGGCGCGGATGTTCGGCCTCTACCCCCGCAAGGGG
ATCGTCGCGCCCGGCTCGGACGCCGACATCGTCATCTACGACCCGGCGGCCAGCACGACCATCTCCGCGTCGACGCACCA
CATGAACCTCGACTACTCCGCGTGGGAGGGCTTCCAGGTCGCGGGAAAGGTCGACACCGTCCTCTCCCGCGGCACGGTCG
TCGTCGCCGGCGGGGAGTTCCACGGGCGGGCCGGGCACGGGCGTTACCTGCGCCGCGGCCTGTCCCAGTACCTGCGATGA

Upstream 100 bases:

>100_bases
GACACCTACGGCGACCTCACCGCGCCGTGACCGGTCGCGTGCCGTCCCGACCCGGGCCGGCAGCCAGGTGGGAAGCAGGT
CCGGTGAAGGGGCAGACGAC

Downstream 100 bases:

>100_bases
GCGTGACGGTGGCGGGCGTGCCTGCGATGAGAACGCCGGTGGCGGGCGTGTCGGTGGCGAGCTGGAAGGCGGATGGCTGA
TGGACGTCGGAGTGGTGCTG

Product: phenylhydantoinase

Products: 3-ureidopropanoate

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MRTLITGGTLVGPLGATPTEILVDGETVAALFAPGSLPEGLRGSLHDGPGSSADRVIDATGKYVIPGGVDVHTHMELPFG
GTSASDTFATGTAAAAWGGTTTIIDFAVQRTGEVVEESLAAWHAKAEGQCAIDYGFHMIIGGVDDQALKAMDYLVANEGI
SSFKLFMAYPGVFYSDDGQILRAMQRAAESGAMVMMHAENGIAIDVLAAQALARGGTGPVHHGYTRPPELEGEATNRAAV
LARVAGNCPLYFVHMSASEALAVVSAARGAGHNVFAETCPQYLYLSLEEHLGAPGFEGAKYVASPPLRTNTASHHDDLWR
GLRTDDLAVVSTDHCPFCFRDQKELGRGDFTRIPNGIGTVEHRIDLVYQGVASGRLSLPRWVETCATTPARMFGLYPRKG
IVAPGSDADIVIYDPAASTTISASTHHMNLDYSAWEGFQVAGKVDTVLSRGTVVVAGGEFHGRAGHGRYLRRGLSQYLR

Sequences:

>Translated_479_residues
MRTLITGGTLVGPLGATPTEILVDGETVAALFAPGSLPEGLRGSLHDGPGSSADRVIDATGKYVIPGGVDVHTHMELPFG
GTSASDTFATGTAAAAWGGTTTIIDFAVQRTGEVVEESLAAWHAKAEGQCAIDYGFHMIIGGVDDQALKAMDYLVANEGI
SSFKLFMAYPGVFYSDDGQILRAMQRAAESGAMVMMHAENGIAIDVLAAQALARGGTGPVHHGYTRPPELEGEATNRAAV
LARVAGNCPLYFVHMSASEALAVVSAARGAGHNVFAETCPQYLYLSLEEHLGAPGFEGAKYVASPPLRTNTASHHDDLWR
GLRTDDLAVVSTDHCPFCFRDQKELGRGDFTRIPNGIGTVEHRIDLVYQGVASGRLSLPRWVETCATTPARMFGLYPRKG
IVAPGSDADIVIYDPAASTTISASTHHMNLDYSAWEGFQVAGKVDTVLSRGTVVVAGGEFHGRAGHGRYLRRGLSQYLR
>Mature_479_residues
MRTLITGGTLVGPLGATPTEILVDGETVAALFAPGSLPEGLRGSLHDGPGSSADRVIDATGKYVIPGGVDVHTHMELPFG
GTSASDTFATGTAAAAWGGTTTIIDFAVQRTGEVVEESLAAWHAKAEGQCAIDYGFHMIIGGVDDQALKAMDYLVANEGI
SSFKLFMAYPGVFYSDDGQILRAMQRAAESGAMVMMHAENGIAIDVLAAQALARGGTGPVHHGYTRPPELEGEATNRAAV
LARVAGNCPLYFVHMSASEALAVVSAARGAGHNVFAETCPQYLYLSLEEHLGAPGFEGAKYVASPPLRTNTASHHDDLWR
GLRTDDLAVVSTDHCPFCFRDQKELGRGDFTRIPNGIGTVEHRIDLVYQGVASGRLSLPRWVETCATTPARMFGLYPRKG
IVAPGSDADIVIYDPAASTTISASTHHMNLDYSAWEGFQVAGKVDTVLSRGTVVVAGGEFHGRAGHGRYLRRGLSQYLR

Specific function: Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin derivatives [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503375, Length=485, Percent_Identity=40, Blast_Score=325, Evalue=7e-89,
Organism=Homo sapiens, GI4503051, Length=427, Percent_Identity=36.0655737704918, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI62422571, Length=427, Percent_Identity=36.0655737704918, Blast_Score=269, Evalue=4e-72,
Organism=Homo sapiens, GI4503379, Length=417, Percent_Identity=34.7721822541966, Blast_Score=262, Evalue=7e-70,
Organism=Homo sapiens, GI4503377, Length=419, Percent_Identity=36.0381861575179, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI19923821, Length=472, Percent_Identity=33.6864406779661, Blast_Score=255, Evalue=8e-68,
Organism=Homo sapiens, GI190194363, Length=421, Percent_Identity=35.3919239904988, Blast_Score=251, Evalue=1e-66,
Organism=Escherichia coli, GI87082175, Length=477, Percent_Identity=40.8805031446541, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1786722, Length=414, Percent_Identity=30.1932367149758, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71989490, Length=472, Percent_Identity=38.5593220338983, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI17539558, Length=474, Percent_Identity=39.662447257384, Blast_Score=295, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI86575075, Length=417, Percent_Identity=28.2973621103118, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI193204318, Length=417, Percent_Identity=23.7410071942446, Blast_Score=85, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6322218, Length=406, Percent_Identity=32.512315270936, Blast_Score=143, Evalue=5e-35,
Organism=Drosophila melanogaster, GI221377917, Length=422, Percent_Identity=41.2322274881517, Blast_Score=291, Evalue=8e-79,
Organism=Drosophila melanogaster, GI17137462, Length=417, Percent_Identity=39.568345323741, Blast_Score=289, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24644287, Length=264, Percent_Identity=40.530303030303, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24644289, Length=188, Percent_Identity=42.5531914893617, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI18859883, Length=459, Percent_Identity=27.2331154684096, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24642586, Length=409, Percent_Identity=22.7383863080685, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011778
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.2

Molecular weight: Translated: 50687; Mature: 50687

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLITGGTLVGPLGATPTEILVDGETVAALFAPGSLPEGLRGSLHDGPGSSADRVIDAT
CCEEEECCCEECCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECC
GKYVIPGGVDVHTHMELPFGGTSASDTFATGTAAAAWGGTTTIIDFAVQRTGEVVEESLA
CCEECCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
AWHAKAEGQCAIDYGFHMIIGGVDDQALKAMDYLVANEGISSFKLFMAYPGVFYSDDGQI
HHHCCCCCCEEEECCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEECCCHHH
LRAMQRAAESGAMVMMHAENGIAIDVLAAQALARGGTGPVHHGYTRPPELEGEATNRAAV
HHHHHHHHCCCCEEEEECCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
LARVAGNCPLYFVHMSASEALAVVSAARGAGHNVFAETCPQYLYLSLEEHLGAPGFEGAK
HHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHCCHHEEEEHHHHCCCCCCCCCC
YVASPPLRTNTASHHDDLWRGLRTDDLAVVSTDHCPFCFRDQKELGRGDFTRIPNGIGTV
EEECCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHCCCCHHHCCCCCCHH
EHRIDLVYQGVASGRLSLPRWVETCATTPARMFGLYPRKGIVAPGSDADIVIYDPAASTT
HHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHCCCCCCCEECCCCCCCEEEECCCCCCE
ISASTHHMNLDYSAWEGFQVAGKVDTVLSRGTVVVAGGEFHGRAGHGRYLRRGLSQYLR
EECCEEEEECCCCCCCCEEECCHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRTLITGGTLVGPLGATPTEILVDGETVAALFAPGSLPEGLRGSLHDGPGSSADRVIDAT
CCEEEECCCEECCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECC
GKYVIPGGVDVHTHMELPFGGTSASDTFATGTAAAAWGGTTTIIDFAVQRTGEVVEESLA
CCEECCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
AWHAKAEGQCAIDYGFHMIIGGVDDQALKAMDYLVANEGISSFKLFMAYPGVFYSDDGQI
HHHCCCCCCEEEECCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEECCCHHH
LRAMQRAAESGAMVMMHAENGIAIDVLAAQALARGGTGPVHHGYTRPPELEGEATNRAAV
HHHHHHHHCCCCEEEEECCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
LARVAGNCPLYFVHMSASEALAVVSAARGAGHNVFAETCPQYLYLSLEEHLGAPGFEGAK
HHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHCCHHEEEEHHHHCCCCCCCCCC
YVASPPLRTNTASHHDDLWRGLRTDDLAVVSTDHCPFCFRDQKELGRGDFTRIPNGIGTV
EEECCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHCCCCHHHCCCCCCHH
EHRIDLVYQGVASGRLSLPRWVETCATTPARMFGLYPRKGIVAPGSDADIVIYDPAASTT
HHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHCCCCCCCEECCCCCCCEEEECCCCCCE
ISASTHHMNLDYSAWEGFQVAGKVDTVLSRGTVVVAGGEFHGRAGHGRYLRRGLSQYLR
EECCEEEEECCCCCCCCEEECCHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,6-dihydrouracil; H2O

Specific reaction: 5,6-dihydrouracil + H2O = 3-ureidopropanoate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]