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The map label for this gene is gcvH [H]

Identifier: 158316760

GI number: 158316760

Start: 5995059

End: 5995439

Strand: Reverse

Name: gcvH [H]

Synonym: Franean1_4998

Alternate gene names: 158316760

Gene position: 5995439-5995059 (Counterclockwise)

Preceding gene: 158316761

Following gene: 158316759

Centisome position: 66.75

GC content: 71.13

Gene sequence:

>381_bases
GTGTACCCGGACGACCTGAAGTACACCCGTGAGCACGAGTGGGCCCGGGCCGAGGGGAACAGGGTGCGGGTCGGCATCAC
CGCGTACGCGCAGGAGGCACTCGGCGACATCGTCTTCGTGTCGCTGCCCGAGGTCGGGCAGGCCGTCTCGGCCGGGGAGC
CGTGCGGCGAGGTCGAGTCGACCAAGAGCGTCTCCGACATCTACGCGCCCGTCTCCGGCGAGGTCGCGGCCCGCAACGAG
GCGCTCGTCGCCGCTCCCGAGCTGGTGAACTCCGACCCCTACGGGGAGGGCTGGCTGGTCGAGGTCACGGTGGTGGACGC
GGCCGTCCTCGACGACCTGCTCGACGCCACCGCCTACCGCGAGCACGTCAAGGAGCAGTAG

Upstream 100 bases:

>100_bases
GACGACGCCGTCGCGGTGGGCGCCGGCCGTCCGGACGGTCCCATGCGTCCGTCGACACCCGTGCCGGCGCTCGGCACCAC
GACCGCAGGAGGCGATTCCG

Downstream 100 bases:

>100_bases
CCGCGCGTGAAGTTCGTGGCCGGCGTGAAGTTCGTGGCCGGACTGTCATTCTGGGACAGGCGTTCCCGGGGCGGGCGCGG
TCATTGGGCGGTGCTATGCG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MYPDDLKYTREHEWARAEGNRVRVGITAYAQEALGDIVFVSLPEVGQAVSAGEPCGEVESTKSVSDIYAPVSGEVAARNE
ALVAAPELVNSDPYGEGWLVEVTVVDAAVLDDLLDATAYREHVKEQ

Sequences:

>Translated_126_residues
MYPDDLKYTREHEWARAEGNRVRVGITAYAQEALGDIVFVSLPEVGQAVSAGEPCGEVESTKSVSDIYAPVSGEVAARNE
ALVAAPELVNSDPYGEGWLVEVTVVDAAVLDDLLDATAYREHVKEQ
>Mature_126_residues
MYPDDLKYTREHEWARAEGNRVRVGITAYAQEALGDIVFVSLPEVGQAVSAGEPCGEVESTKSVSDIYAPVSGEVAARNE
ALVAAPELVNSDPYGEGWLVEVTVVDAAVLDDLLDATAYREHVKEQ

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=118, Percent_Identity=44.9152542372881, Blast_Score=113, Evalue=4e-26,
Organism=Homo sapiens, GI89057342, Length=118, Percent_Identity=44.0677966101695, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1789271, Length=125, Percent_Identity=55.2, Blast_Score=139, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17507493, Length=119, Percent_Identity=42.8571428571429, Blast_Score=104, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17551294, Length=119, Percent_Identity=40.3361344537815, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=103, Percent_Identity=45.6310679611651, Blast_Score=97, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17865652, Length=115, Percent_Identity=42.6086956521739, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13608; Mature: 13608

Theoretical pI: Translated: 3.97; Mature: 3.97

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPDDLKYTREHEWARAEGNRVRVGITAYAQEALGDIVFVSLPEVGQAVSAGEPCGEVES
CCCCCCCCCCCCCHHCCCCCEEEEEHHHHHHHHHCCEEEEECCCCCCHHCCCCCCCCCCC
TKSVSDIYAPVSGEVAARNEALVAAPELVNSDPYGEGWLVEVTVVDAAVLDDLLDATAYR
CCCHHHHHCCCCCCEECCCCEEEECHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHH
EHVKEQ
HHHCCC
>Mature Secondary Structure
MYPDDLKYTREHEWARAEGNRVRVGITAYAQEALGDIVFVSLPEVGQAVSAGEPCGEVES
CCCCCCCCCCCCCHHCCCCCEEEEEHHHHHHHHHCCEEEEECCCCCCHHCCCCCCCCCCC
TKSVSDIYAPVSGEVAARNEALVAAPELVNSDPYGEGWLVEVTVVDAAVLDDLLDATAYR
CCCHHHHHCCCCCCEECCCCEEEECHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHH
EHVKEQ
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA