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The map label for this gene is paaF [C]

Identifier: 158316337

GI number: 158316337

Start: 5448924

End: 5449730

Strand: Reverse

Name: paaF [C]

Synonym: Franean1_4566

Alternate gene names: 158316337

Gene position: 5449730-5448924 (Counterclockwise)

Preceding gene: 158316338

Following gene: 158316328

Centisome position: 60.67

GC content: 72.37

Gene sequence:

>807_bases
ATGGCCGACGTCAGCCAGACCCGTCGGTCCGAGACCGACGGGATCCTGACGCTCACCTTCACACGCGACGAGAAGCTCAA
CGCCGTCAGCCCCGAGATGATCGAGGCGCTGCGCCAGGCCGCGACCGATCTGGGCGAGCGCGACGACCTCAAGGTGCTCG
TGATCGCGGCGGAAGGCCGCTACTTCACGGCCGGCATCGACATCCGCCAGAGCGGCGCCGGTGGCAAGCGCGGGTACGCC
GCGGACGGCAGCTTCAGCCCGCGGCGGCTGCGCCGTGAGTACCGGCGGCTGCACCTGGTCTTCGACGAGCTGGAGGCGGT
CGAGAAGCCCGTCGTGCTGGCCGCGCACGCGCCGTGCCTGGGCGTCGGCCTCGAACTGGCCGCGTCCTGCGACTTCCGGC
TGGCGTCCACCGCCGCGGTGTTCTCCCTGCCCGAACTGCCCGCGCTCGCCGTCATCCCGGGCTCCGGGGGCATCAGCCGA
GTGACCAGGCTGGTCGGCCCGCACTGGGGCAAGTGGCTCGCGTTCGGGGAGGAGGTCGACGCCCACCACGCCCGGGACAT
CGGGCTCGTGCACGCCGTCTACCCGGCCGAGGAGTTCTCCGAGCGGGTGAGCGCCTTCGCCCGCAAGCTGGCCCGGCTGC
CCTCGGAGGCGGCGGGCGTGGCGAAGGCGGCCGTCGACATCGCCGCGAGCGTCGACCGGCAGACGGCGCGCGACTTCGAC
CGCTTCGCCAACACCATCCTGTTGACGTCGGACGAGCACCGGGCGCTGACCCGGGCCTTCGAGACGCGCAAGAAGTCCGC
TGAGTAG

Upstream 100 bases:

>100_bases
GCGGTGCCGCGCCGCTCGAGGCCGGCCACCGGGCCGGCACCGAGCGACACCGGGCAGGGGCACCACAGGAACGCCATCCC
TACGAGAAGCGGGAGCCGTT

Downstream 100 bases:

>100_bases
ATCTGACGTCCAGTGGCTCTGGCGTCGAGCAGATCTTCGGCGTCGAGGGGACCTGATGCCTAGGGGTGCTGACGCTGCCT
GCGTCAGCACCCCTCGGTGT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MADVSQTRRSETDGILTLTFTRDEKLNAVSPEMIEALRQAATDLGERDDLKVLVIAAEGRYFTAGIDIRQSGAGGKRGYA
ADGSFSPRRLRREYRRLHLVFDELEAVEKPVVLAAHAPCLGVGLELAASCDFRLASTAAVFSLPELPALAVIPGSGGISR
VTRLVGPHWGKWLAFGEEVDAHHARDIGLVHAVYPAEEFSERVSAFARKLARLPSEAAGVAKAAVDIAASVDRQTARDFD
RFANTILLTSDEHRALTRAFETRKKSAE

Sequences:

>Translated_268_residues
MADVSQTRRSETDGILTLTFTRDEKLNAVSPEMIEALRQAATDLGERDDLKVLVIAAEGRYFTAGIDIRQSGAGGKRGYA
ADGSFSPRRLRREYRRLHLVFDELEAVEKPVVLAAHAPCLGVGLELAASCDFRLASTAAVFSLPELPALAVIPGSGGISR
VTRLVGPHWGKWLAFGEEVDAHHARDIGLVHAVYPAEEFSERVSAFARKLARLPSEAAGVAKAAVDIAASVDRQTARDFD
RFANTILLTSDEHRALTRAFETRKKSAE
>Mature_267_residues
ADVSQTRRSETDGILTLTFTRDEKLNAVSPEMIEALRQAATDLGERDDLKVLVIAAEGRYFTAGIDIRQSGAGGKRGYAA
DGSFSPRRLRREYRRLHLVFDELEAVEKPVVLAAHAPCLGVGLELAASCDFRLASTAAVFSLPELPALAVIPGSGGISRV
TRLVGPHWGKWLAFGEEVDAHHARDIGLVHAVYPAEEFSERVSAFARKLARLPSEAAGVAKAAVDIAASVDRQTARDFDR
FANTILLTSDEHRALTRAFETRKKSAE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=260, Percent_Identity=27.3076923076923, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI68989263, Length=223, Percent_Identity=30.0448430493274, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI70995211, Length=190, Percent_Identity=28.4210526315789, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI31542718, Length=219, Percent_Identity=27.8538812785388, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI20127408, Length=172, Percent_Identity=29.0697674418605, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=30.5882352941176, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=31.6831683168317, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=27.3809523809524, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790281, Length=198, Percent_Identity=29.2929292929293, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=25.2918287937743, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI221142681, Length=215, Percent_Identity=29.7674418604651, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=23.0769230769231, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=26.9230769230769, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=203, Percent_Identity=27.0935960591133, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=185, Percent_Identity=27.5675675675676, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=226, Percent_Identity=28.3185840707965, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=210, Percent_Identity=26.1904761904762, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=213, Percent_Identity=24.8826291079812, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=29.0697674418605, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20129971, Length=232, Percent_Identity=26.7241379310345, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24653477, Length=232, Percent_Identity=26.7241379310345, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19920382, Length=181, Percent_Identity=29.2817679558011, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29123; Mature: 28992

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVSQTRRSETDGILTLTFTRDEKLNAVSPEMIEALRQAATDLGERDDLKVLVIAAEGR
CCCCHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCE
YFTAGIDIRQSGAGGKRGYAADGSFSPRRLRREYRRLHLVFDELEAVEKPVVLAAHAPCL
EEEECCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHH
GVGLELAASCDFRLASTAAVFSLPELPALAVIPGSGGISRVTRLVGPHWGKWLAFGEEVD
HHCHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHCCCHHH
AHHARDIGLVHAVYPAEEFSERVSAFARKLARLPSEAAGVAKAAVDIAASVDRQTARDFD
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RFANTILLTSDEHRALTRAFETRKKSAE
HHHCEEEEECCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADVSQTRRSETDGILTLTFTRDEKLNAVSPEMIEALRQAATDLGERDDLKVLVIAAEGR
CCCHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCE
YFTAGIDIRQSGAGGKRGYAADGSFSPRRLRREYRRLHLVFDELEAVEKPVVLAAHAPCL
EEEECCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHH
GVGLELAASCDFRLASTAAVFSLPELPALAVIPGSGGISRVTRLVGPHWGKWLAFGEEVD
HHCHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHCCCHHH
AHHARDIGLVHAVYPAEEFSERVSAFARKLARLPSEAAGVAKAAVDIAASVDRQTARDFD
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RFANTILLTSDEHRALTRAFETRKKSAE
HHHCEEEEECCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA