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The map label for this gene is fadA [H]

Identifier: 158316328

GI number: 158316328

Start: 5435821

End: 5436984

Strand: Reverse

Name: fadA [H]

Synonym: Franean1_4557

Alternate gene names: 158316328

Gene position: 5436984-5435821 (Counterclockwise)

Preceding gene: 158316337

Following gene: 158316327

Centisome position: 60.53

GC content: 71.65

Gene sequence:

>1164_bases
ATGAAGGGGGTTACCGTGCGGGACGCCGTCATCGTCGAAGCGGTACGGACGCCGATCGGTCGGCGCAACGGCACGCTGCG
GGGCGTTCACCCGGTCGATCTGGCCTCGGACGTGCTCCGGACGCTCACCGAGCGTGCGCAGGTCGCCCCCGAACTCGTCG
ACGACGTCATCTTTGGCTGCGTCACTCAGATCGCCGACCAGTCGACGAACGTCGCCCGCCTGGCCGTGCTGGCCGCCGGC
TGGCCGGAAAGCATCCCGGGGGTGACGCTGGACCGGGCCTGCGGCTCGAGTCAGCAGGCGGTCCACTTCGCCGCGGCCGG
GGTGATGGCGGGCCAGTACGACCTCGTCGTCGCCGGCGGCGTCGAGTCGATGAGCCGGGTTCCGCTGGGTTCGGCCCGGG
CCGTCGGTGAGCCGTTCGGCCCCCGGGTGCGCGCGCGCTACCAGCGGGACAGCTTCAGTCAGGGTCTCGGCGCCGAGGAG
ATCGCGCACCGGTGGGGCCTGTCCCGGACCCGGCTCGACGAGTTCGCCCTGGAGTCGCACCACCGCGCGATGGCGGCCGT
CGACTCGGGCGCGTTCGACGCGCAGCTCGTTCCCGTCGAGGGACCGGACGGCGTCCTCACCGCCGACGAGGGGGTGCGGC
GGGAGACGACGCTCGAGAAGCTCGGTGCGCTGAAGCCCGCGTTCCGCGAGGACGGGGTGATCCACGCGGGCAACAGTTCC
CAGATCTCCGACGGCAGCGCGGCACTGCTGATCACGACCAGCGAACGGGCGGCCGAGCTGGGCCTGCGACCGCTCGCTCG
CTTCCACGCCGGTACGGTCGCCGGTGACGATCCGGTGATCATGTTGACCGCTCCGATCCCCGCGACGGAGAAGGTCCTGA
AGCGGGCCGGGCTCACGATCGCCGACATCGGTGTGTACGAGGTGAACGAGGCGTTCGCGAGCGTTCCGCTCGCCTGGGAG
ATCGAGACGGGCGCCGACCACGCCCGGCTGAACCCGCTCGGGGGCGCGATCGCGGTGGGCCACCCGCTCGGCGCCTCGGG
CGCGATCCTGATGACCCGCATGATCCACCACATGCGCGACCACGGCATCCGCTACGGCCTGCAGACCATGTGCGAGGCCG
GCGGCATGGCCAACGCGACCGTCATCGAGCTGATCGGTGCCTGA

Upstream 100 bases:

>100_bases
CCGACCTCGGCGCCGGTCTCGAGGGCGGCCACGTTGACAGGCGGGGAGTCGGCTCCTAGCTTGTTCTCTCAGTTGAATTA
TGTTCAAGGTAGTGAACTCG

Downstream 100 bases:

>100_bases
GGCCGGGCCGGGCCCGGGCCGCGGCATCGACGGTTTCTCCCGCCCGTCGCCCCGAGGAGCGTCATGAACCTGAAGTCCGT
CCCGGCATTCGTCACCGGCG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKGVTVRDAVIVEAVRTPIGRRNGTLRGVHPVDLASDVLRTLTERAQVAPELVDDVIFGCVTQIADQSTNVARLAVLAAG
WPESIPGVTLDRACGSSQQAVHFAAAGVMAGQYDLVVAGGVESMSRVPLGSARAVGEPFGPRVRARYQRDSFSQGLGAEE
IAHRWGLSRTRLDEFALESHHRAMAAVDSGAFDAQLVPVEGPDGVLTADEGVRRETTLEKLGALKPAFREDGVIHAGNSS
QISDGSAALLITTSERAAELGLRPLARFHAGTVAGDDPVIMLTAPIPATEKVLKRAGLTIADIGVYEVNEAFASVPLAWE
IETGADHARLNPLGGAIAVGHPLGASGAILMTRMIHHMRDHGIRYGLQTMCEAGGMANATVIELIGA

Sequences:

>Translated_387_residues
MKGVTVRDAVIVEAVRTPIGRRNGTLRGVHPVDLASDVLRTLTERAQVAPELVDDVIFGCVTQIADQSTNVARLAVLAAG
WPESIPGVTLDRACGSSQQAVHFAAAGVMAGQYDLVVAGGVESMSRVPLGSARAVGEPFGPRVRARYQRDSFSQGLGAEE
IAHRWGLSRTRLDEFALESHHRAMAAVDSGAFDAQLVPVEGPDGVLTADEGVRRETTLEKLGALKPAFREDGVIHAGNSS
QISDGSAALLITTSERAAELGLRPLARFHAGTVAGDDPVIMLTAPIPATEKVLKRAGLTIADIGVYEVNEAFASVPLAWE
IETGADHARLNPLGGAIAVGHPLGASGAILMTRMIHHMRDHGIRYGLQTMCEAGGMANATVIELIGA
>Mature_387_residues
MKGVTVRDAVIVEAVRTPIGRRNGTLRGVHPVDLASDVLRTLTERAQVAPELVDDVIFGCVTQIADQSTNVARLAVLAAG
WPESIPGVTLDRACGSSQQAVHFAAAGVMAGQYDLVVAGGVESMSRVPLGSARAVGEPFGPRVRARYQRDSFSQGLGAEE
IAHRWGLSRTRLDEFALESHHRAMAAVDSGAFDAQLVPVEGPDGVLTADEGVRRETTLEKLGALKPAFREDGVIHAGNSS
QISDGSAALLITTSERAAELGLRPLARFHAGTVAGDDPVIMLTAPIPATEKVLKRAGLTIADIGVYEVNEAFASVPLAWE
IETGADHARLNPLGGAIAVGHPLGASGAILMTRMIHHMRDHGIRYGLQTMCEAGGMANATVIELIGA

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=391, Percent_Identity=37.5959079283887, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI167614485, Length=392, Percent_Identity=34.6938775510204, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI148539872, Length=389, Percent_Identity=37.7892030848329, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI4557237, Length=392, Percent_Identity=34.9489795918367, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI4504327, Length=424, Percent_Identity=33.9622641509434, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI194353979, Length=381, Percent_Identity=28.3464566929134, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI48994986, Length=390, Percent_Identity=43.3333333333333, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=43.3915211970075, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1788554, Length=392, Percent_Identity=41.8367346938776, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI87082165, Length=393, Percent_Identity=37.6590330788804, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI1788683, Length=423, Percent_Identity=33.096926713948, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI133906874, Length=391, Percent_Identity=37.5959079283887, Blast_Score=230, Evalue=8e-61,
Organism=Caenorhabditis elegans, GI25147385, Length=389, Percent_Identity=34.4473007712082, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17535921, Length=392, Percent_Identity=34.1836734693878, Blast_Score=197, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=427, Percent_Identity=31.8501170960187, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17535917, Length=400, Percent_Identity=29.5, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6322031, Length=391, Percent_Identity=35.0383631713555, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6325229, Length=402, Percent_Identity=33.8308457711443, Blast_Score=192, Evalue=6e-50,
Organism=Drosophila melanogaster, GI24655093, Length=388, Percent_Identity=36.0824742268041, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI17648125, Length=394, Percent_Identity=35.5329949238579, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI24640423, Length=394, Percent_Identity=35.0253807106599, Blast_Score=208, Evalue=5e-54,
Organism=Drosophila melanogaster, GI17137578, Length=428, Percent_Identity=32.4766355140187, Blast_Score=191, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40627; Mature: 40627

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGVTVRDAVIVEAVRTPIGRRNGTLRGVHPVDLASDVLRTLTERAQVAPELVDDVIFGC
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTQIADQSTNVARLAVLAAGWPESIPGVTLDRACGSSQQAVHFAAAGVMAGQYDLVVAGG
HHHHHCCCCCCEEEEEEECCCCCCCCCCEEHHHCCCCCHHHHHHHHHHCCCCEEEEEECC
VESMSRVPLGSARAVGEPFGPRVRARYQRDSFSQGLGAEEIAHRWGLSRTRLDEFALESH
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
HRAMAAVDSGAFDAQLVPVEGPDGVLTADEGVRRETTLEKLGALKPAFREDGVIHAGNSS
HHHHHHHCCCCCCCEEEECCCCCCEEECCCCCCHHHHHHHHHCCCCCHHCCCEEECCCCC
QISDGSAALLITTSERAAELGLRPLARFHAGTVAGDDPVIMLTAPIPATEKVLKRAGLTI
CCCCCCEEEEEEECHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCEE
ADIGVYEVNEAFASVPLAWEIETGADHARLNPLGGAIAVGHPLGASGAILMTRMIHHMRD
EECCHHHHHHHHHCCCEEEEECCCCCCCEECCCCCEEEECCCCCCCHHHHHHHHHHHHHH
HGIRYGLQTMCEAGGMANATVIELIGA
HHHHHHHHHHHHCCCCCCCCCEEEECC
>Mature Secondary Structure
MKGVTVRDAVIVEAVRTPIGRRNGTLRGVHPVDLASDVLRTLTERAQVAPELVDDVIFGC
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTQIADQSTNVARLAVLAAGWPESIPGVTLDRACGSSQQAVHFAAAGVMAGQYDLVVAGG
HHHHHCCCCCCEEEEEEECCCCCCCCCCEEHHHCCCCCHHHHHHHHHHCCCCEEEEEECC
VESMSRVPLGSARAVGEPFGPRVRARYQRDSFSQGLGAEEIAHRWGLSRTRLDEFALESH
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
HRAMAAVDSGAFDAQLVPVEGPDGVLTADEGVRRETTLEKLGALKPAFREDGVIHAGNSS
HHHHHHHCCCCCCCEEEECCCCCCEEECCCCCCHHHHHHHHHCCCCCHHCCCEEECCCCC
QISDGSAALLITTSERAAELGLRPLARFHAGTVAGDDPVIMLTAPIPATEKVLKRAGLTI
CCCCCCEEEEEEECHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCEE
ADIGVYEVNEAFASVPLAWEIETGADHARLNPLGGAIAVGHPLGASGAILMTRMIHHMRD
EECCHHHHHHHHHCCCEEEEECCCCCCCEECCCCCEEEECCCCCCCHHHHHHHHHHHHHH
HGIRYGLQTMCEAGGMANATVIELIGA
HHHHHHHHHHHHCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA