Click here to switch to the map view.

The map label for this gene is menE [H]

Identifier: 158316302

GI number: 158316302

Start: 5402982

End: 5404571

Strand: Reverse

Name: menE [H]

Synonym: Franean1_4529

Alternate gene names: 158316302

Gene position: 5404571-5402982 (Counterclockwise)

Preceding gene: 158316303

Following gene: 158316301

Centisome position: 60.17

GC content: 71.76

Gene sequence:

>1590_bases
ATGAGCGGCCCGATCCCATCGTGGGGACGCGATGTCACTGTCGAACAGGTAGGCGGCGTTCCGTTCCGAATGTACGAGCC
GCGGGCGCGGAGGCTGGAGTCCCTGCTGGACCATGCCGACCGCTGGGCGGGCCGGGCCCACGCCGTGCAGGGCGACGTGC
GGCTCGACTTCGGCGACCTCGTGCCCGCGGTCGGTCACAAGGCGGCCCAGCTGAGGCAGCACGGGGTGGGCCGTGGGGAC
CGGGTCGCGCTGCTGGGCTGGAACAGCCCGGACTGGGTGGTGAACCTCTGGGCCACCTGGTGGCTCGGGGCCGTCCCGGT
CCTGGTCAACGCCTGGTGGAGCACCCGAGAGATGGAGCACGCCTTCACGGCGCTCACACCGGTCGCCGTGCTCGCCGACC
GCCGGCTGGAGCACAAGGTGCCAGCCGGGAGTCCCGTCGCGCCGTGGCTGATGGCCGACGCGGTCGACGGTGCGCCGCCT
GAGCGTCCCGATAGCGACGACGAGAACGAGCCCGCCCTGATTCTGTTCACCTCCGGCAGCACCGGATTCCCCAAGGCGGT
CGTGCTCTCGCACCGCTCGATCATCTCCGGCCTGCACTCACTGCTGAGAATCACCAAGCGGCTTCCGCAGGAACTCGAGG
GCGCGGCGCCCAGCGTCGCCCTGCACACGGGGCCGCTGTTCCACATCGGCGGCGTACAGACCCTCGTGCGAGGGGTCGTG
GTCGGCGAGACCCTGGTGTTCCCGGAAGGCAAGTTCGACGCAGATGCGGCGATGGACCTCATCTCCGCGCATGGTGTCAC
CCGCTGGAGCGCGGTCCCCACCATGGTCAGCCGGCTGCTCGACGCCCAGGCACAGCGGCCGGTCGACCTGCACAGCCTGC
GGTCACTGACACTGGGCGGCGCCCCCGCCCACCCCAGTCTGTACCAGCGGATCCGCGACGAGCTGCCGTCCGTCCAGGCC
CGCGTGGCCACGGGTTACGGCCTCACCGAGAACGGTGGGCAGGCCACCGCGGCCAGCGGCCGCGACACCCGTGACCGGCC
CGGCTGCTGCGGCCTGCCGCTACCGACCGTCGAGATCTCCTTCGGTGACCGGACGCCGGGCGGGGACGGCGAGGTCCTGC
TCCGCGCCGCGTCGCAGATGCTCGGCTACTACGGGGAGGCGTCCGGCCCCATCGACGCCGAGGGGTGGCTGCACACCGGT
GACCTGGGCTACCTCGACGAGGACGGCTATCTCTGGGTGACCGGTCGCAGCAAGGATCTCATTCTGCGTGGGGGCGAGAA
CATCGCGCCGCTGTCGGTCGAGCGCGCGCTGGTCGGCGTGCCCGGCGTTCTCGACGCGGCGGTGGTCGGCCTGCCGCACG
TCGACCTGGGGGAGGAGGTCGCGGCGGTCGTGGTGGTCGACGAGGCCACCGCGGGCCGGCCGGATCTCGCGGAGTACGTC
ATCGAGGTCCTGCGCTCGGACCTGGCGTCCTTCGCGATCCCGACCCGGTGGCGCTTCCAGACCGAGGAACTGCCGGTGCT
CGGGTCGGAGAAGATCGACAAGCACGCGCTCGCCGCGGAGTTCGCCGCCGAGACCGCCGCTTCCCGGTGA

Upstream 100 bases:

>100_bases
CAAGTCCGTCGAGCCGAATACGCGAGCAGACGGGCAGATGACCATTGCCGAGCCCCGTCGGCCGCATGGCCCGCGTGGAC
GGCGGAGAGAGGAAGCGGCG

Downstream 100 bases:

>100_bases
ACATCCCGGCCTCGGTGCGCGCCGCGTCGGCGCGCACCGAGGCCGGGTACGGCTGCGGCACGACACCCCTGCCGAAGCAG
CGCCTACGAAGCGGCGCCGG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSGPIPSWGRDVTVEQVGGVPFRMYEPRARRLESLLDHADRWAGRAHAVQGDVRLDFGDLVPAVGHKAAQLRQHGVGRGD
RVALLGWNSPDWVVNLWATWWLGAVPVLVNAWWSTREMEHAFTALTPVAVLADRRLEHKVPAGSPVAPWLMADAVDGAPP
ERPDSDDENEPALILFTSGSTGFPKAVVLSHRSIISGLHSLLRITKRLPQELEGAAPSVALHTGPLFHIGGVQTLVRGVV
VGETLVFPEGKFDADAAMDLISAHGVTRWSAVPTMVSRLLDAQAQRPVDLHSLRSLTLGGAPAHPSLYQRIRDELPSVQA
RVATGYGLTENGGQATAASGRDTRDRPGCCGLPLPTVEISFGDRTPGGDGEVLLRAASQMLGYYGEASGPIDAEGWLHTG
DLGYLDEDGYLWVTGRSKDLILRGGENIAPLSVERALVGVPGVLDAAVVGLPHVDLGEEVAAVVVVDEATAGRPDLAEYV
IEVLRSDLASFAIPTRWRFQTEELPVLGSEKIDKHALAAEFAAETAASR

Sequences:

>Translated_529_residues
MSGPIPSWGRDVTVEQVGGVPFRMYEPRARRLESLLDHADRWAGRAHAVQGDVRLDFGDLVPAVGHKAAQLRQHGVGRGD
RVALLGWNSPDWVVNLWATWWLGAVPVLVNAWWSTREMEHAFTALTPVAVLADRRLEHKVPAGSPVAPWLMADAVDGAPP
ERPDSDDENEPALILFTSGSTGFPKAVVLSHRSIISGLHSLLRITKRLPQELEGAAPSVALHTGPLFHIGGVQTLVRGVV
VGETLVFPEGKFDADAAMDLISAHGVTRWSAVPTMVSRLLDAQAQRPVDLHSLRSLTLGGAPAHPSLYQRIRDELPSVQA
RVATGYGLTENGGQATAASGRDTRDRPGCCGLPLPTVEISFGDRTPGGDGEVLLRAASQMLGYYGEASGPIDAEGWLHTG
DLGYLDEDGYLWVTGRSKDLILRGGENIAPLSVERALVGVPGVLDAAVVGLPHVDLGEEVAAVVVVDEATAGRPDLAEYV
IEVLRSDLASFAIPTRWRFQTEELPVLGSEKIDKHALAAEFAAETAASR
>Mature_528_residues
SGPIPSWGRDVTVEQVGGVPFRMYEPRARRLESLLDHADRWAGRAHAVQGDVRLDFGDLVPAVGHKAAQLRQHGVGRGDR
VALLGWNSPDWVVNLWATWWLGAVPVLVNAWWSTREMEHAFTALTPVAVLADRRLEHKVPAGSPVAPWLMADAVDGAPPE
RPDSDDENEPALILFTSGSTGFPKAVVLSHRSIISGLHSLLRITKRLPQELEGAAPSVALHTGPLFHIGGVQTLVRGVVV
GETLVFPEGKFDADAAMDLISAHGVTRWSAVPTMVSRLLDAQAQRPVDLHSLRSLTLGGAPAHPSLYQRIRDELPSVQAR
VATGYGLTENGGQATAASGRDTRDRPGCCGLPLPTVEISFGDRTPGGDGEVLLRAASQMLGYYGEASGPIDAEGWLHTGD
LGYLDEDGYLWVTGRSKDLILRGGENIAPLSVERALVGVPGVLDAAVVGLPHVDLGEEVAAVVVVDEATAGRPDLAEYVI
EVLRSDLASFAIPTRWRFQTEELPVLGSEKIDKHALAAEFAAETAASR

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=521, Percent_Identity=27.447216890595, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI187761345, Length=506, Percent_Identity=29.2490118577075, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI187761343, Length=506, Percent_Identity=29.2490118577075, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI42544132, Length=365, Percent_Identity=26.027397260274, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI27477105, Length=491, Percent_Identity=24.2362525458248, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI8923896, Length=525, Percent_Identity=24.7619047619048, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI122937307, Length=373, Percent_Identity=27.6139410187668, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI28416953, Length=527, Percent_Identity=23.9089184060721, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI253970489, Length=370, Percent_Identity=27.8378378378378, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI38505220, Length=482, Percent_Identity=24.896265560166, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI115511026, Length=392, Percent_Identity=27.0408163265306, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI83745141, Length=181, Percent_Identity=32.5966850828729, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI157311624, Length=369, Percent_Identity=25.4742547425474, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI157311622, Length=369, Percent_Identity=25.4742547425474, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI58082049, Length=369, Percent_Identity=25.2032520325203, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI195947378, Length=294, Percent_Identity=26.530612244898, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI145693145, Length=358, Percent_Identity=29.8882681564246, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1788107, Length=367, Percent_Identity=28.3378746594005, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI221142682, Length=529, Percent_Identity=24.007561436673, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788595, Length=469, Percent_Identity=28.1449893390192, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786810, Length=525, Percent_Identity=26.2857142857143, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790505, Length=396, Percent_Identity=25.2525252525253, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17558820, Length=487, Percent_Identity=28.5420944558522, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32563687, Length=478, Percent_Identity=25.5230125523013, Blast_Score=161, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17560308, Length=518, Percent_Identity=27.2200772200772, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI71994703, Length=572, Percent_Identity=25.3496503496504, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI71994694, Length=572, Percent_Identity=25.3496503496504, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI71994690, Length=572, Percent_Identity=25.3496503496504, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI71996755, Length=505, Percent_Identity=23.7623762376238, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=330, Percent_Identity=29.6969696969697, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=498, Percent_Identity=23.8955823293173, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=520, Percent_Identity=27.1153846153846, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17557194, Length=509, Percent_Identity=26.7190569744597, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI25141359, Length=497, Percent_Identity=22.5352112676056, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71985884, Length=349, Percent_Identity=28.6532951289398, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71983001, Length=507, Percent_Identity=25.4437869822485, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71982997, Length=507, Percent_Identity=25.4437869822485, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17560140, Length=330, Percent_Identity=23.6363636363636, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=384, Percent_Identity=28.90625, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319264, Length=389, Percent_Identity=27.5064267352185, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323182, Length=527, Percent_Identity=24.2884250474383, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24581924, Length=524, Percent_Identity=27.8625954198473, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI21355181, Length=429, Percent_Identity=27.039627039627, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24648260, Length=477, Percent_Identity=24.5283018867925, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21356441, Length=443, Percent_Identity=25.7336343115124, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24648255, Length=466, Percent_Identity=25.5364806866953, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24648253, Length=466, Percent_Identity=25.3218884120172, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21358303, Length=535, Percent_Identity=23.7383177570093, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922652, Length=511, Percent_Identity=22.3091976516634, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24653035, Length=445, Percent_Identity=25.3932584269663, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648257, Length=494, Percent_Identity=24.2914979757085, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281365686, Length=461, Percent_Identity=22.9934924078091, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356947, Length=472, Percent_Identity=24.5762711864407, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI161076582, Length=409, Percent_Identity=24.9388753056235, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24667955, Length=422, Percent_Identity=24.8815165876777, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24667959, Length=422, Percent_Identity=24.8815165876777, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24656500, Length=370, Percent_Identity=25.6756756756757, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI62472339, Length=422, Percent_Identity=24.8815165876777, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI18859661, Length=493, Percent_Identity=24.1379310344828, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20130357, Length=444, Percent_Identity=24.7747747747748, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17933690, Length=167, Percent_Identity=28.1437125748503, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921316, Length=170, Percent_Identity=30, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 56640; Mature: 56508

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPIPSWGRDVTVEQVGGVPFRMYEPRARRLESLLDHADRWAGRAHAVQGDVRLDFGDL
CCCCCCCCCCCEEHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCCEEECCCEEECHHHH
VPAVGHKAAQLRQHGVGRGDRVALLGWNSPDWVVNLWATWWLGAVPVLVNAWWSTREMEH
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFTALTPVAVLADRRLEHKVPAGSPVAPWLMADAVDGAPPERPDSDDENEPALILFTSGS
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCC
TGFPKAVVLSHRSIISGLHSLLRITKRLPQELEGAAPSVALHTGPLFHIGGVQTLVRGVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCHHHHHHHHH
VGETLVFPEGKFDADAAMDLISAHGVTRWSAVPTMVSRLLDAQAQRPVDLHSLRSLTLGG
HCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
APAHPSLYQRIRDELPSVQARVATGYGLTENGGQATAASGRDTRDRPGCCGLPLPTVEIS
CCCCHHHHHHHHHHCCCHHEEHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEE
FGDRTPGGDGEVLLRAASQMLGYYGEASGPIDAEGWLHTGDLGYLDEDGYLWVTGRSKDL
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEECCCCEEEEECCCCEE
ILRGGENIAPLSVERALVGVPGVLDAAVVGLPHVDLGEEVAAVVVVDEATAGRPDLAEYV
EEECCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHHHH
IEVLRSDLASFAIPTRWRFQTEELPVLGSEKIDKHALAAEFAAETAASR
HHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGPIPSWGRDVTVEQVGGVPFRMYEPRARRLESLLDHADRWAGRAHAVQGDVRLDFGDL
CCCCCCCCCCEEHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCCEEECCCEEECHHHH
VPAVGHKAAQLRQHGVGRGDRVALLGWNSPDWVVNLWATWWLGAVPVLVNAWWSTREMEH
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFTALTPVAVLADRRLEHKVPAGSPVAPWLMADAVDGAPPERPDSDDENEPALILFTSGS
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCC
TGFPKAVVLSHRSIISGLHSLLRITKRLPQELEGAAPSVALHTGPLFHIGGVQTLVRGVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCHHHHHHHHH
VGETLVFPEGKFDADAAMDLISAHGVTRWSAVPTMVSRLLDAQAQRPVDLHSLRSLTLGG
HCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
APAHPSLYQRIRDELPSVQARVATGYGLTENGGQATAASGRDTRDRPGCCGLPLPTVEIS
CCCCHHHHHHHHHHCCCHHEEHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEE
FGDRTPGGDGEVLLRAASQMLGYYGEASGPIDAEGWLHTGDLGYLDEDGYLWVTGRSKDL
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEECCCCEEEEECCCCEE
ILRGGENIAPLSVERALVGVPGVLDAAVVGLPHVDLGEEVAAVVVVDEATAGRPDLAEYV
EEECCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHHHH
IEVLRSDLASFAIPTRWRFQTEELPVLGSEKIDKHALAAEFAAETAASR
HHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA