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The map label for this gene is menB [C]

Identifier: 158316079

GI number: 158316079

Start: 5135110

End: 5135901

Strand: Reverse

Name: menB [C]

Synonym: Franean1_4297

Alternate gene names: 158316079

Gene position: 5135901-5135110 (Counterclockwise)

Preceding gene: 158316080

Following gene: 158316078

Centisome position: 57.18

GC content: 68.06

Gene sequence:

>792_bases
TTGTTAGTCCTATACGACGTCGCCGACGGGATCGCGACGATCACGCTCAACCGCCCCGAGGTCGCGAACGCGCAGAACGG
ACCGCTCCTCGAGGAGCTCGACGCGGCGTGGACGAAGGCGGCGGCCGACAAGGACGTGGCGGTGATCGTGCTGCGCGCGG
AGGGGAAGCACTTCTCCGCCGGGCACGACCTGAAGTCCGGTCAGCCGCTCCCCGAGAAAATCACCCTGGAGATGCTCTAC
GAGCTGGAGGCACGGCGCTACCTGGAGTTCTCGCTGCGGTGGCGCAACGTTCCCAAGCCGTCGATCGCGGCGGTGCAGGG
GCGCTGCATCTCGGGCGGGCTGCTGCTGTGCTGGCCGTGCGACCTGATCATCGCGGCCGAGGACGCGCTGTTCTCCGACC
CCGTCGTCGCCATGGGCATAGGCGGTGTCGAGTACCACGGGCACACCTGGGAGCTCGGCCCGCGCAAGGCCAAGGAGATC
CTCTTCACCGGGCGCGCGGTGACGGCCTCCGAGGCCGAGAAGATCGGCATGGTCAACAAGGTGGTGCCCCGCGACCAGCT
CGACACCGAGACCCGCGCGCTGGCCCAGCACATCGCGACGATGCCCAGCTTCGGGCTCCGGCAGGCCAAGCGCGCGGTCA
ACCAGACCCTCGACGTGCAGGGCTTCTACGCCGCGATCCAGTCGGTGTTCGACGTCCACCAGACCGGGCACGGCAACGCG
CTGAGCGTCAGCGGATGGCCGGTCCTGGTCAACCTGGACCAGATGAAGTCAGCGATCAAGAAGGCCGACTAG

Upstream 100 bases:

>100_bases
AGGTCCTTCGCCGGGACCATCGCCGGTGGCACCTCGGAGATCCAGCGCACGATCGTCGCCGAGCGTGTACTCGGTCTGCC
GCGAAGCTGAGGAGCCAGCC

Downstream 100 bases:

>100_bases
GCCCGAGACACAGCCGACGGCGGCGGGCGGGGGCGCGTGACGGCCCCGCCGGCCGCCTTTAGCCGCGCGTCGCCCGCCGA
CGCAGGCACAGCCCGGGAGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MLVLYDVADGIATITLNRPEVANAQNGPLLEELDAAWTKAAADKDVAVIVLRAEGKHFSAGHDLKSGQPLPEKITLEMLY
ELEARRYLEFSLRWRNVPKPSIAAVQGRCISGGLLLCWPCDLIIAAEDALFSDPVVAMGIGGVEYHGHTWELGPRKAKEI
LFTGRAVTASEAEKIGMVNKVVPRDQLDTETRALAQHIATMPSFGLRQAKRAVNQTLDVQGFYAAIQSVFDVHQTGHGNA
LSVSGWPVLVNLDQMKSAIKKAD

Sequences:

>Translated_263_residues
MLVLYDVADGIATITLNRPEVANAQNGPLLEELDAAWTKAAADKDVAVIVLRAEGKHFSAGHDLKSGQPLPEKITLEMLY
ELEARRYLEFSLRWRNVPKPSIAAVQGRCISGGLLLCWPCDLIIAAEDALFSDPVVAMGIGGVEYHGHTWELGPRKAKEI
LFTGRAVTASEAEKIGMVNKVVPRDQLDTETRALAQHIATMPSFGLRQAKRAVNQTLDVQGFYAAIQSVFDVHQTGHGNA
LSVSGWPVLVNLDQMKSAIKKAD
>Mature_263_residues
MLVLYDVADGIATITLNRPEVANAQNGPLLEELDAAWTKAAADKDVAVIVLRAEGKHFSAGHDLKSGQPLPEKITLEMLY
ELEARRYLEFSLRWRNVPKPSIAAVQGRCISGGLLLCWPCDLIIAAEDALFSDPVVAMGIGGVEYHGHTWELGPRKAKEI
LFTGRAVTASEAEKIGMVNKVVPRDQLDTETRALAQHIATMPSFGLRQAKRAVNQTLDVQGFYAAIQSVFDVHQTGHGNA
LSVSGWPVLVNLDQMKSAIKKAD

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=213, Percent_Identity=31.924882629108, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI31542718, Length=231, Percent_Identity=32.034632034632, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI45643119, Length=209, Percent_Identity=30.1435406698565, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI260275230, Length=209, Percent_Identity=30.1435406698565, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI260274832, Length=209, Percent_Identity=30.1435406698565, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI70995211, Length=186, Percent_Identity=31.7204301075269, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI68989263, Length=222, Percent_Identity=28.3783783783784, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1788597, Length=215, Percent_Identity=35.3488372093023, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787659, Length=210, Percent_Identity=30.952380952381, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787660, Length=227, Percent_Identity=31.7180616740088, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082183, Length=195, Percent_Identity=30.2564102564103, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI221142681, Length=232, Percent_Identity=31.0344827586207, Blast_Score=67, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=213, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=246, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=203, Percent_Identity=24.6305418719212, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=218, Percent_Identity=24.3119266055046, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=216, Percent_Identity=25.462962962963, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=210, Percent_Identity=29.0476190476191, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129971, Length=213, Percent_Identity=36.150234741784, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24653477, Length=213, Percent_Identity=36.150234741784, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24653139, Length=220, Percent_Identity=30.9090909090909, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920382, Length=223, Percent_Identity=29.5964125560538, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922422, Length=198, Percent_Identity=30.3030303030303, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21357171, Length=235, Percent_Identity=27.6595744680851, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24650670, Length=230, Percent_Identity=29.5652173913043, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28635; Mature: 28635

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVLYDVADGIATITLNRPEVANAQNGPLLEELDAAWTKAAADKDVAVIVLRAEGKHFSA
CEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
GHDLKSGQPLPEKITLEMLYELEARRYLEFSLRWRNVPKPSIAAVQGRCISGGLLLCWPC
CCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHCCEEECCCEEEEECC
DLIIAAEDALFSDPVVAMGIGGVEYHGHTWELGPRKAKEILFTGRAVTASEAEKIGMVNK
CEEEEECCCCCCCCEEEEECCCEEECCCEECCCCHHHHHHEEECCEECHHHHHHHCHHHH
VVPRDQLDTETRALAQHIATMPSFGLRQAKRAVNQTLDVQGFYAAIQSVFDVHQTGHGNA
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCE
LSVSGWPVLVNLDQMKSAIKKAD
EEECCCEEEEEHHHHHHHHHCCC
>Mature Secondary Structure
MLVLYDVADGIATITLNRPEVANAQNGPLLEELDAAWTKAAADKDVAVIVLRAEGKHFSA
CEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
GHDLKSGQPLPEKITLEMLYELEARRYLEFSLRWRNVPKPSIAAVQGRCISGGLLLCWPC
CCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHCCEEECCCEEEEECC
DLIIAAEDALFSDPVVAMGIGGVEYHGHTWELGPRKAKEILFTGRAVTASEAEKIGMVNK
CEEEEECCCCCCCCEEEEECCCEEECCCEECCCCHHHHHHEEECCEECHHHHHHHCHHHH
VVPRDQLDTETRALAQHIATMPSFGLRQAKRAVNQTLDVQGFYAAIQSVFDVHQTGHGNA
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCE
LSVSGWPVLVNLDQMKSAIKKAD
EEECCCEEEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA