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The map label for this gene is bphF [H]

Identifier: 158314945

GI number: 158314945

Start: 3690617

End: 3691381

Strand: Direct

Name: bphF [H]

Synonym: Franean1_3136

Alternate gene names: 158314945

Gene position: 3690617-3691381 (Clockwise)

Preceding gene: 158314944

Following gene: 158314946

Centisome position: 41.09

GC content: 68.1

Gene sequence:

>765_bases
ATGACGGAGTCCATCGTGCCCGCGCGCTCGACCTCCCGGTCGCTACGGTGCCGCCTCGCTGATGGCGAGCGAATGCAAGG
CATCATTTTCCCCTTACCCTTCACCCTCGAGCTCGAGATCGCGGCGCGTTGCCGAGTCGACTTCTTCGTCGCCGACCTCG
AGCACGGGCCCGCGGACTACGTGCAGCTGCAACAGCACATCGCGCTGGCAGCGGCCCACGGCTGCGACGTCCTCGTTCGG
ACACATGCCCACGACCCGTCCGGTATCTCCCGCATCCTCGATCTGGGCGCGGCCGGTTTGATGTTCCCGAGAGTGGGCTC
CAGGGCCGACGCCGAGCGTCTCGTTGCCCTGGCAACATACCCGCCCGGCGGTGCTCGGGGCCTGTCAACCAGCACGCCCG
CGGCGCACTACGGTCGGTGGTCGGCCGCGCGGACAGTTGCCGACGCCCGCGAGCGGACTCTGTTGATCGCCATGGTGGAG
ACGGTCGAGGGTGTTGAGGACGCCTACGGGATCGCCAGCGTCGACGGCATCGACGCGCTCTTCGTGGGGACCGCTGACCT
CACCGCGTCGATGGAGATGCCGGGCGCGGTCGGTGATCCTCGGGTACAGGAGCTCGCCCGCAGCGTCGCGCCGCAGGCGC
AACGTGCAGGCAAGTGGTTGCTGGAGGAGGCGACCGGCTCGACCAGCAGCCAGTTCGTTGTTCACAACGGAACCAAGCTC
CTGACCGGTGCGTTCGAGCGCGCCTTCGGAACGCGGAAGACCTGA

Upstream 100 bases:

>100_bases
TGTCGGTGTCCTGATTTTAAACGCCCAGGTGAGCGGCCTGCACCGGCCGATGACGGAGCGGTCCACCCCTGGCGCGAGCC
CATCACATAAGGGAGCGGAG

Downstream 100 bases:

>100_bases
TGCCCGTCGCGCCGCCGCCTCGGGCACCAATGGCGGCTGGCCCGTCTGGAGCGAGGAGAAGCAACCCGAGAATCGGGAAC
CTCGACGGACGACCGGTCCT

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: HOA [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTESIVPARSTSRSLRCRLADGERMQGIIFPLPFTLELEIAARCRVDFFVADLEHGPADYVQLQQHIALAAAHGCDVLVR
THAHDPSGISRILDLGAAGLMFPRVGSRADAERLVALATYPPGGARGLSTSTPAAHYGRWSAARTVADARERTLLIAMVE
TVEGVEDAYGIASVDGIDALFVGTADLTASMEMPGAVGDPRVQELARSVAPQAQRAGKWLLEEATGSTSSQFVVHNGTKL
LTGAFERAFGTRKT

Sequences:

>Translated_254_residues
MTESIVPARSTSRSLRCRLADGERMQGIIFPLPFTLELEIAARCRVDFFVADLEHGPADYVQLQQHIALAAAHGCDVLVR
THAHDPSGISRILDLGAAGLMFPRVGSRADAERLVALATYPPGGARGLSTSTPAAHYGRWSAARTVADARERTLLIAMVE
TVEGVEDAYGIASVDGIDALFVGTADLTASMEMPGAVGDPRVQELARSVAPQAQRAGKWLLEEATGSTSSQFVVHNGTKL
LTGAFERAFGTRKT
>Mature_253_residues
TESIVPARSTSRSLRCRLADGERMQGIIFPLPFTLELEIAARCRVDFFVADLEHGPADYVQLQQHIALAAAHGCDVLVRT
HAHDPSGISRILDLGAAGLMFPRVGSRADAERLVALATYPPGGARGLSTSTPAAHYGRWSAARTVADARERTLLIAMVET
VEGVEDAYGIASVDGIDALFVGTADLTASMEMPGAVGDPRVQELARSVAPQAQRAGKWLLEEATGSTSSQFVVHNGTKLL
TGAFERAFGTRKT

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-oxovalerate to pyruvate and acetaldehyde (Probable) [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=219, Percent_Identity=29.2237442922374, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788578, Length=208, Percent_Identity=30.2884615384615, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 27055; Mature: 26924

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESIVPARSTSRSLRCRLADGERMQGIIFPLPFTLELEIAARCRVDFFVADLEHGPADY
CCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCEEEEEEEEEEEEEEEEEEECCCCCHHH
VQLQQHIALAAAHGCDVLVRTHAHDPSGISRILDLGAAGLMFPRVGSRADAERLVALATY
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEEEC
PPGGARGLSTSTPAAHYGRWSAARTVADARERTLLIAMVETVEGVEDAYGIASVDGIDAL
CCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCEEEEEHHHHHHHHHHHCCCCCCCCCCEE
FVGTADLTASMEMPGAVGDPRVQELARSVAPQAQRAGKWLLEEATGSTSSQFVVHNGTKL
EEECCCCEEECCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCEEEEECCCCH
LTGAFERAFGTRKT
HHHHHHHHCCCCCC
>Mature Secondary Structure 
TESIVPARSTSRSLRCRLADGERMQGIIFPLPFTLELEIAARCRVDFFVADLEHGPADY
CCCCCCCCCCCCEEEEEECCCCCCCEEEECCCEEEEEEEEEEEEEEEEEEECCCCCHHH
VQLQQHIALAAAHGCDVLVRTHAHDPSGISRILDLGAAGLMFPRVGSRADAERLVALATY
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEEEC
PPGGARGLSTSTPAAHYGRWSAARTVADARERTLLIAMVETVEGVEDAYGIASVDGIDAL
CCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCEEEEEHHHHHHHHHHHCCCCCCCCCCEE
FVGTADLTASMEMPGAVGDPRVQELARSVAPQAQRAGKWLLEEATGSTSSQFVVHNGTKL
EEECCCCEEECCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCEEEEECCCCH
LTGAFERAFGTRKT
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9073078 [H]