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The map label for this gene is paaF [C]

Identifier: 158314944

GI number: 158314944

Start: 3689680

End: 3690504

Strand: Direct

Name: paaF [C]

Synonym: Franean1_3135

Alternate gene names: 158314944

Gene position: 3689680-3690504 (Clockwise)

Preceding gene: 158314943

Following gene: 158314945

Centisome position: 41.08

GC content: 66.55

Gene sequence:

>825_bases
GTGAGCTCGTGGGTGAAACTGGTTGTAGACGAGGACGAGGCCGCGGGCATCGTGTGTATCACGCTCAACCGACCTGAGAA
GCGGAACGCGCTGGACACCGAGCTGCTGAGTGAGTATGTCAGTGTCCTGGAGTCGTATCTGGGCTCCCCGACAATCCGGG
TCATCGTAACCCAGGGCGCGGGGACGGTGTTCTGCGCGGGAATGGACCTGCACGATCTCAAGGACTGGGTGCACAGGTGG
GACACCGGGGAGGTGGCATGGTCCGACACCGGACTCCTGTCGAGGGCGATGCGGCTGCTGCGTGAGCACACCTGCATCAC
GATCGCCTCCGTTCAGGGCGTGTGCGCCGGCGGCGGGATCGCCCTGGTTGTGGCCCACGACCTAGCGGTCGCCTCGGACA
CCGCGCGGTTCATCCTGCCGGACACGGCGCGGGGGAGTTTCGGTGCCGCCGCGGCCGCCGCGGCCCATTTCGCCGTCCCG
ACCAAGGTCGCCTTCGACATACAGCTCACCGGTCGCGAGCTCCTCGGACGGGAGGCGATGTCGCTGGGTCTGGTTTCTCG
ATCGGTACCGCTGGATCGGCTGCCCGCCGAGACGGAGGAGGTCGCCGCCCAGGTGGCGAGCCGGCAACGGGAGCCGCTGA
TCCACGCCAAGATGGCGCGTCACCTGAACGAAGGCCGGCCTTTGACCGACACCATGAGCATCGATCTGCTCGTCCGGACA
AGGCAGGACGTCGCCCGGAACTCTTTCAACGACGTCACCGGATTCCTTGCGTCGCGTAAGGGTGCGGTGGACGCCTCACC
GGGGGCCTCACAGGCGATTAGCTAG

Upstream 100 bases:

>100_bases
GGCGGGCAGCCCGAGCCGCCGGCGGAGTGGTGATGCTTCAACTCGATGGCAGGGCTCGTGGAAGTTGGGGATTCGCGGCC
CGACAGGGGAGGAGAGTTTC

Downstream 100 bases:

>100_bases
CCGGCTTCGTCATGTCGGTGTCCTGATTTTAAACGCCCAGGTGAGCGGCCTGCACCGGCCGATGACGGAGCGGTCCACCC
CTGGCGCGAGCCCATCACAT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSSWVKLVVDEDEAAGIVCITLNRPEKRNALDTELLSEYVSVLESYLGSPTIRVIVTQGAGTVFCAGMDLHDLKDWVHRW
DTGEVAWSDTGLLSRAMRLLREHTCITIASVQGVCAGGGIALVVAHDLAVASDTARFILPDTARGSFGAAAAAAAHFAVP
TKVAFDIQLTGRELLGREAMSLGLVSRSVPLDRLPAETEEVAAQVASRQREPLIHAKMARHLNEGRPLTDTMSIDLLVRT
RQDVARNSFNDVTGFLASRKGAVDASPGASQAIS

Sequences:

>Translated_274_residues
MSSWVKLVVDEDEAAGIVCITLNRPEKRNALDTELLSEYVSVLESYLGSPTIRVIVTQGAGTVFCAGMDLHDLKDWVHRW
DTGEVAWSDTGLLSRAMRLLREHTCITIASVQGVCAGGGIALVVAHDLAVASDTARFILPDTARGSFGAAAAAAAHFAVP
TKVAFDIQLTGRELLGREAMSLGLVSRSVPLDRLPAETEEVAAQVASRQREPLIHAKMARHLNEGRPLTDTMSIDLLVRT
RQDVARNSFNDVTGFLASRKGAVDASPGASQAIS
>Mature_273_residues
SSWVKLVVDEDEAAGIVCITLNRPEKRNALDTELLSEYVSVLESYLGSPTIRVIVTQGAGTVFCAGMDLHDLKDWVHRWD
TGEVAWSDTGLLSRAMRLLREHTCITIASVQGVCAGGGIALVVAHDLAVASDTARFILPDTARGSFGAAAAAAAHFAVPT
KVAFDIQLTGRELLGREAMSLGLVSRSVPLDRLPAETEEVAAQVASRQREPLIHAKMARHLNEGRPLTDTMSIDLLVRTR
QDVARNSFNDVTGFLASRKGAVDASPGASQAIS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=31.7460317460317, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI70995211, Length=185, Percent_Identity=28.1081081081081, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI194097323, Length=230, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594469, Length=186, Percent_Identity=26.8817204301075, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI37594471, Length=186, Percent_Identity=26.8817204301075, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI45643119, Length=242, Percent_Identity=26.8595041322314, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI260275230, Length=242, Percent_Identity=26.8595041322314, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI260274832, Length=242, Percent_Identity=26.8595041322314, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=209, Percent_Identity=32.5358851674641, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788597, Length=192, Percent_Identity=32.8125, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI221142681, Length=198, Percent_Identity=30.3030303030303, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=221, Percent_Identity=30.316742081448, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=249, Percent_Identity=27.710843373494, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=218, Percent_Identity=27.5229357798165, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=194, Percent_Identity=29.3814432989691, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=240, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=190, Percent_Identity=25.2631578947368, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=190, Percent_Identity=25.2631578947368, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129971, Length=196, Percent_Identity=29.5918367346939, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653477, Length=196, Percent_Identity=29.5918367346939, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21357171, Length=213, Percent_Identity=26.7605633802817, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24650670, Length=223, Percent_Identity=30.4932735426009, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29315; Mature: 29184

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSWVKLVVDEDEAAGIVCITLNRPEKRNALDTELLSEYVSVLESYLGSPTIRVIVTQGA
CCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
GTVFCAGMDLHDLKDWVHRWDTGEVAWSDTGLLSRAMRLLREHTCITIASVQGVCAGGGI
CEEEEECCCHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCEEEEEECCCEECCCCE
ALVVAHDLAVASDTARFILPDTARGSFGAAAAAAAHFAVPTKVAFDIQLTGRELLGREAM
EEEEEHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHH
SLGLVSRSVPLDRLPAETEEVAAQVASRQREPLIHAKMARHLNEGRPLTDTMSIDLLVRT
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHEEEEEEEEC
RQDVARNSFNDVTGFLASRKGAVDASPGASQAIS
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSWVKLVVDEDEAAGIVCITLNRPEKRNALDTELLSEYVSVLESYLGSPTIRVIVTQGA
CCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
GTVFCAGMDLHDLKDWVHRWDTGEVAWSDTGLLSRAMRLLREHTCITIASVQGVCAGGGI
CEEEEECCCHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCEEEEEECCCEECCCCE
ALVVAHDLAVASDTARFILPDTARGSFGAAAAAAAHFAVPTKVAFDIQLTGRELLGREAM
EEEEEHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHH
SLGLVSRSVPLDRLPAETEEVAAQVASRQREPLIHAKMARHLNEGRPLTDTMSIDLLVRT
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHEEEEEEEEC
RQDVARNSFNDVTGFLASRKGAVDASPGASQAIS
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA