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The map label for this gene is pcm [H]

Identifier: 158314622

GI number: 158314622

Start: 3315505

End: 3316701

Strand: Direct

Name: pcm [H]

Synonym: Franean1_2801

Alternate gene names: 158314622

Gene position: 3315505-3316701 (Clockwise)

Preceding gene: 158314621

Following gene: 158314623

Centisome position: 36.91

GC content: 69.09

Gene sequence:

>1197_bases
ATGGACACCACCGCAGCCACATCACCAGGCACGCTGCGCGACCGCATGGTCGACCGCATCCTCACCAGCCAGAATCTGCC
CCCGTGGGTCGAAACGGCGCTGCGTTCCGTCAAACGCCACCGCTACGTCCCCGAAGCGCCGCTGGCCGACGCCTACGACG
AGAAGGCGGTCATCACCCACACCTTCCCCGACGGCACCCACCTCAGCTGCGCCTCCGGCCCCACCATCGTGGCCGCCATG
CTCACCGCCCTCGATGTCCGGCCCGATCAGCACATCCTGGAGATCGGCGCCGGCACCGGCTACAACGCGGCCCTCCTCGC
CACCCTCGTGGGCACCGGCGGCCAGGTCACCACTATCGACATCAACGCCGACGTCACCGCCGCAGCACGGCGCAACCTTG
ACGACACCGGCTTTCCCTACGTTCGCGTTCTCACCCGCGACGGCGCTGACGGCGCTGCCGAGGACGGCCCCTTCGATCGG
ATCATCGTCACGGTCGGCGCCTGGGACATCCCACAGGCCTGGTGGGACCAGCTCGTCCCCGATGGCCGCCTCGTCCTCCC
GCTGCGCTGGCGCGGCACTACCCGCGCTGTCGCACTCACCAGGCAGGAAGACCACTGGAAGTCCGACTGGGTCTTCCTGT
GCGGCTTTGTGCCGATGCTCGGCCAGCCCGGCGAGCGGAGGAGCGTCATCCACCCCGACGGCCTCGCCGCCCTGCACCAC
GATCTCGACCAACCCATCGACACCGACGCCCTGCGCGGTGTCCTCGACCGGGAGAAGTCCGTCGTCTGGTCTGACGTGAC
CGTGCACGGTCAGGAACCCTTCGACCGCGTCTGGCTGCACCTCAGCGCCGTGGAAGACGGCACGGTCCGCATCGAGGCCG
ACCAGCAGGCCGTCGCCGAGGGCCTGTGCACACCCGCGATCGCCTCACGCAGCCCAGCCCTGGTCAAAGACGGTTCACTG
GCCTACTTCACCATCCGGCGCGCCGACACCCCAGGGCGCTGGCAGCTCGGCGCCATCGGCCACGGGCCCCTCGGTCGCCG
TCTTGCCTCACGGATCGTCGACCAGATCGACGCCTGGGACCACGACCGCACTGCCGACCCCGAAATCCTCGCCTTCCCGG
CCGGCACGCCGATCCCGAACCAGACGCAAGGCAAGATCATAACCAAGCCGGAGAACCGCCTGGTACTGCGCTACTAG

Upstream 100 bases:

>100_bases
GCTGCCAGACTGGGACCGCTGCCTGTTGATCAACTAGCAGTCGGTTACCCGGCCGAGGCCGCTGTCACCACCACGCCCTC
TGCCGCTGTGAAGGAGACTG

Downstream 100 bases:

>100_bases
TGGTACGCAACTACTCCGGTTCGGGGCGCCTGTCGCCCGCGCTCCTGCGCGACATCCCGGCAGGTTGATCAGTATCGGCC
GGCGTCAGGACGGACAAGTG

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MDTTAATSPGTLRDRMVDRILTSQNLPPWVETALRSVKRHRYVPEAPLADAYDEKAVITHTFPDGTHLSCASGPTIVAAM
LTALDVRPDQHILEIGAGTGYNAALLATLVGTGGQVTTIDINADVTAAARRNLDDTGFPYVRVLTRDGADGAAEDGPFDR
IIVTVGAWDIPQAWWDQLVPDGRLVLPLRWRGTTRAVALTRQEDHWKSDWVFLCGFVPMLGQPGERRSVIHPDGLAALHH
DLDQPIDTDALRGVLDREKSVVWSDVTVHGQEPFDRVWLHLSAVEDGTVRIEADQQAVAEGLCTPAIASRSPALVKDGSL
AYFTIRRADTPGRWQLGAIGHGPLGRRLASRIVDQIDAWDHDRTADPEILAFPAGTPIPNQTQGKIITKPENRLVLRY

Sequences:

>Translated_398_residues
MDTTAATSPGTLRDRMVDRILTSQNLPPWVETALRSVKRHRYVPEAPLADAYDEKAVITHTFPDGTHLSCASGPTIVAAM
LTALDVRPDQHILEIGAGTGYNAALLATLVGTGGQVTTIDINADVTAAARRNLDDTGFPYVRVLTRDGADGAAEDGPFDR
IIVTVGAWDIPQAWWDQLVPDGRLVLPLRWRGTTRAVALTRQEDHWKSDWVFLCGFVPMLGQPGERRSVIHPDGLAALHH
DLDQPIDTDALRGVLDREKSVVWSDVTVHGQEPFDRVWLHLSAVEDGTVRIEADQQAVAEGLCTPAIASRSPALVKDGSL
AYFTIRRADTPGRWQLGAIGHGPLGRRLASRIVDQIDAWDHDRTADPEILAFPAGTPIPNQTQGKIITKPENRLVLRY
>Mature_398_residues
MDTTAATSPGTLRDRMVDRILTSQNLPPWVETALRSVKRHRYVPEAPLADAYDEKAVITHTFPDGTHLSCASGPTIVAAM
LTALDVRPDQHILEIGAGTGYNAALLATLVGTGGQVTTIDINADVTAAARRNLDDTGFPYVRVLTRDGADGAAEDGPFDR
IIVTVGAWDIPQAWWDQLVPDGRLVLPLRWRGTTRAVALTRQEDHWKSDWVFLCGFVPMLGQPGERRSVIHPDGLAALHH
DLDQPIDTDALRGVLDREKSVVWSDVTVHGQEPFDRVWLHLSAVEDGTVRIEADQQAVAEGLCTPAIASRSPALVKDGSL
AYFTIRRADTPGRWQLGAIGHGPLGRRLASRIVDQIDAWDHDRTADPEILAFPAGTPIPNQTQGKIITKPENRLVLRY

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=159, Percent_Identity=35.8490566037736, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 43522; Mature: 43522

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTTAATSPGTLRDRMVDRILTSQNLPPWVETALRSVKRHRYVPEAPLADAYDEKAVITH
CCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
TFPDGTHLSCASGPTIVAAMLTALDVRPDQHILEIGAGTGYNAALLATLVGTGGQVTTID
ECCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEE
INADVTAAARRNLDDTGFPYVRVLTRDGADGAAEDGPFDRIIVTVGAWDIPQAWWDQLVP
ECCCEEHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCC
DGRLVLPLRWRGTTRAVALTRQEDHWKSDWVFLCGFVPMLGQPGERRSVIHPDGLAALHH
CCEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCHHHHHH
DLDQPIDTDALRGVLDREKSVVWSDVTVHGQEPFDRVWLHLSAVEDGTVRIEADQQAVAE
HHCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCHHHEEEEEEEECCCEEEEECCHHHHHH
GLCTPAIASRSPALVKDGSLAYFTIRRADTPGRWQLGAIGHGPLGRRLASRIVDQIDAWD
CCCCCHHCCCCCCEEECCCEEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCC
HDRTADPEILAFPAGTPIPNQTQGKIITKPENRLVLRY
CCCCCCCCEEEECCCCCCCCCCCCEEEECCCCCEEEEC
>Mature Secondary Structure
MDTTAATSPGTLRDRMVDRILTSQNLPPWVETALRSVKRHRYVPEAPLADAYDEKAVITH
CCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
TFPDGTHLSCASGPTIVAAMLTALDVRPDQHILEIGAGTGYNAALLATLVGTGGQVTTID
ECCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEE
INADVTAAARRNLDDTGFPYVRVLTRDGADGAAEDGPFDRIIVTVGAWDIPQAWWDQLVP
ECCCEEHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCC
DGRLVLPLRWRGTTRAVALTRQEDHWKSDWVFLCGFVPMLGQPGERRSVIHPDGLAALHH
CCEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCHHHHHH
DLDQPIDTDALRGVLDREKSVVWSDVTVHGQEPFDRVWLHLSAVEDGTVRIEADQQAVAE
HHCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCHHHEEEEEEEECCCEEEEECCHHHHHH
GLCTPAIASRSPALVKDGSLAYFTIRRADTPGRWQLGAIGHGPLGRRLASRIVDQIDAWD
CCCCCHHCCCCCCEEECCCEEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCC
HDRTADPEILAFPAGTPIPNQTQGKIITKPENRLVLRY
CCCCCCCCEEEECCCCCCCCCCCCEEEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]