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The map label for this gene is pgk

Identifier: 158313899

GI number: 158313899

Start: 2487733

End: 2488935

Strand: Direct

Name: pgk

Synonym: Franean1_2064

Alternate gene names: 158313899

Gene position: 2487733-2488935 (Clockwise)

Preceding gene: 158313898

Following gene: 158313900

Centisome position: 27.7

GC content: 73.73

Gene sequence:

>1203_bases
GTGAGGACGATCGACGACCTGCAGGTCACCGGTCACCGCGTACTTGTCCGCAGTGATCTCAACGTGCCCCTGGACCATTC
CAAGGGCGCGCCGCGCATCACCGACGACGGCCGGGTGCGCGCGAGCGTGCCGACCATCCAGGCCCTTCTGGACCGGAACG
CGAAGGTGATCGTCTGCTCGCATCTGGGACGTCCCAAGGGGGCCCCGGAGGAGAAGTACTCCCTCGCCCCGGTCGCCGAG
CGGCTCGCCGAGCTGCTCGGCATCCCGGTGGCGTTCGCCGGGGACGGCGGCGGCGACATCGCCGGTGACCGGGCCCGGGA
GGTGGTCGGGCAGCTCGCCGAGGGCCAGGTGGCCCTGCTGGAGAACCTGCGCTTCCACCCGGGCGAGACCAGCAAGGACA
CCGTCGCCCGCGCGACCTTCGCCGACGAGCTCTCGGCGCTCGCCGAGTTCTACGTCGGCGACGCGTTCGGCGCGGTGCAC
CGGGCCCACGCGAGCGTGTCGGAGGTGCCCAAGCGCCTCCCGCACGCCGCCGGCCGGCTGGTGCTGACCGAGCTCGAGGT
GCTGCGGGCGCTGACCGCGGCGCCGGCCCGGCCGTACGCCGTCGTGCTCGGCGGGTCGAAGGTGTCGGACAAGCTCGGCG
TCATCCGGGCCCTGCTGCCGAAGGTGGACGCGCTCCTCGTCGGCGGCGGCATGTGCTTCACCTTCCTGGCGGCCCTCGGG
CACCCGGTCGGCGCCTCGCTCCTGGAGAGCGAGATGATCGACACCTGCAAGGACCTGCTGGCCGAGGCCGGTGACCGGCT
CGTGCTGCCCACCGACGTGGTGGTCGCCGACCGGTTCGCCGCCGACGCGGAGACCGCCGTCGTCGCCGCCGACGCGATCC
CGGACGGCTGGCTCGGCCTCGACATCGGGCCGGCATCCACCGCCGCGTTCGCGCGCGTGGTCGCCGGTGCGGCGACGATC
TTCTGGAACGGCCCGATGGGCGTGTTCGAGTTCGCGCCGTTCGCGGAGGGTACCCGCGGGGTCGCCGAGGCGGTCGCGTC
CGGCGGCGGCTTCTCGGTCGTTGGCGGCGGCGACTCGGCCGCCGCGGTGCGCATCCTCGGCATCCCGGAGGACGACTTCA
GCCACATCTCCACCGGCGGCGGCGCGAGCCTGGAGTACCTGGAGGGCAAGACGCTCCCGGGCCTCGCCGCGCTCGACGTC
TGA

Upstream 100 bases:

>100_bases
CAACCGGCTCGTCGACCTGACCGGGCTCGTCGCCGCCCGGATCTGACATCGGCGAACCGGCCCGTCCATCGCACCTTCGC
CCACCCCGGAGCAGCCCAGC

Downstream 100 bases:

>100_bases
GTACCACCGAGTCTGATCTTCACACTTTCACCGAGGAAGGGAAGCAGGGACGTGGCGAAGGGGGCACCCGCCCGCGGGTC
GGCCGGAACCGGCCGGCGGA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MRTIDDLQVTGHRVLVRSDLNVPLDHSKGAPRITDDGRVRASVPTIQALLDRNAKVIVCSHLGRPKGAPEEKYSLAPVAE
RLAELLGIPVAFAGDGGGDIAGDRAREVVGQLAEGQVALLENLRFHPGETSKDTVARATFADELSALAEFYVGDAFGAVH
RAHASVSEVPKRLPHAAGRLVLTELEVLRALTAAPARPYAVVLGGSKVSDKLGVIRALLPKVDALLVGGGMCFTFLAALG
HPVGASLLESEMIDTCKDLLAEAGDRLVLPTDVVVADRFAADAETAVVAADAIPDGWLGLDIGPASTAAFARVVAGAATI
FWNGPMGVFEFAPFAEGTRGVAEAVASGGGFSVVGGGDSAAAVRILGIPEDDFSHISTGGGASLEYLEGKTLPGLAALDV

Sequences:

>Translated_400_residues
MRTIDDLQVTGHRVLVRSDLNVPLDHSKGAPRITDDGRVRASVPTIQALLDRNAKVIVCSHLGRPKGAPEEKYSLAPVAE
RLAELLGIPVAFAGDGGGDIAGDRAREVVGQLAEGQVALLENLRFHPGETSKDTVARATFADELSALAEFYVGDAFGAVH
RAHASVSEVPKRLPHAAGRLVLTELEVLRALTAAPARPYAVVLGGSKVSDKLGVIRALLPKVDALLVGGGMCFTFLAALG
HPVGASLLESEMIDTCKDLLAEAGDRLVLPTDVVVADRFAADAETAVVAADAIPDGWLGLDIGPASTAAFARVVAGAATI
FWNGPMGVFEFAPFAEGTRGVAEAVASGGGFSVVGGGDSAAAVRILGIPEDDFSHISTGGGASLEYLEGKTLPGLAALDV
>Mature_400_residues
MRTIDDLQVTGHRVLVRSDLNVPLDHSKGAPRITDDGRVRASVPTIQALLDRNAKVIVCSHLGRPKGAPEEKYSLAPVAE
RLAELLGIPVAFAGDGGGDIAGDRAREVVGQLAEGQVALLENLRFHPGETSKDTVARATFADELSALAEFYVGDAFGAVH
RAHASVSEVPKRLPHAAGRLVLTELEVLRALTAAPARPYAVVLGGSKVSDKLGVIRALLPKVDALLVGGGMCFTFLAALG
HPVGASLLESEMIDTCKDLLAEAGDRLVLPTDVVVADRFAADAETAVVAADAIPDGWLGLDIGPASTAAFARVVAGAATI
FWNGPMGVFEFAPFAEGTRGVAEAVASGGGFSVVGGGDSAAAVRILGIPEDDFSHISTGGGASLEYLEGKTLPGLAALDV

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI31543397, Length=418, Percent_Identity=43.7799043062201, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI4505763, Length=418, Percent_Identity=43.3014354066986, Blast_Score=306, Evalue=3e-83,
Organism=Escherichia coli, GI1789294, Length=398, Percent_Identity=43.4673366834171, Blast_Score=285, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17508823, Length=415, Percent_Identity=44.3373493975904, Blast_Score=309, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6319857, Length=419, Percent_Identity=43.6754176610979, Blast_Score=301, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24581203, Length=417, Percent_Identity=39.8081534772182, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI17136394, Length=418, Percent_Identity=41.3875598086124, Blast_Score=272, Evalue=3e-73,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_FRASN (A8KYR5)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001506407.1
- ProteinModelPortal:   A8KYR5
- SMR:   A8KYR5
- GeneID:   5670465
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_2064
- HOGENOM:   HBG453500
- OMA:   EMHETAR
- ProtClustDB:   PRK00073
- BioCyc:   FSP1855:FRANEAN1_2064-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41182; Mature: 41182

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 38-38 BINDING 124-124 BINDING 161-161 BINDING 211-211 BINDING 299-299 BINDING 330-330

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIDDLQVTGHRVLVRSDLNVPLDHSKGAPRITDDGRVRASVPTIQALLDRNAKVIVCS
CCCCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHCCCCCEEEEE
HLGRPKGAPEEKYSLAPVAERLAELLGIPVAFAGDGGGDIAGDRAREVVGQLAEGQVALL
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCHHHHH
ENLRFHPGETSKDTVARATFADELSALAEFYVGDAFGAVHRAHASVSEVPKRLPHAAGRL
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHH
VLTELEVLRALTAAPARPYAVVLGGSKVSDKLGVIRALLPKVDALLVGGGMCFTFLAALG
HHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHC
HPVGASLLESEMIDTCKDLLAEAGDRLVLPTDVVVADRFAADAETAVVAADAIPDGWLGL
CCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHCCCCCCEEEEECCCCCCCEEE
DIGPASTAAFARVVAGAATIFWNGPMGVFEFAPFAEGTRGVAEAVASGGGFSVVGGGDSA
ECCCCHHHHHHHHHHHHEEEEECCCCCHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCC
AAVRILGIPEDDFSHISTGGGASLEYLEGKTLPGLAALDV
EEEEEEECCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MRTIDDLQVTGHRVLVRSDLNVPLDHSKGAPRITDDGRVRASVPTIQALLDRNAKVIVCS
CCCCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHCCCCCEEEEE
HLGRPKGAPEEKYSLAPVAERLAELLGIPVAFAGDGGGDIAGDRAREVVGQLAEGQVALL
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCHHHHH
ENLRFHPGETSKDTVARATFADELSALAEFYVGDAFGAVHRAHASVSEVPKRLPHAAGRL
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHH
VLTELEVLRALTAAPARPYAVVLGGSKVSDKLGVIRALLPKVDALLVGGGMCFTFLAALG
HHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHC
HPVGASLLESEMIDTCKDLLAEAGDRLVLPTDVVVADRFAADAETAVVAADAIPDGWLGL
CCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHCCCCCCEEEEECCCCCCCEEE
DIGPASTAAFARVVAGAATIFWNGPMGVFEFAPFAEGTRGVAEAVASGGGFSVVGGGDSA
ECCCCHHHHHHHHHHHHEEEEECCCCCHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCC
AAVRILGIPEDDFSHISTGGGASLEYLEGKTLPGLAALDV
EEEEEEECCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA