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The map label for this gene is hisH [H]

Identifier: 158313754

GI number: 158313754

Start: 2300392

End: 2301006

Strand: Direct

Name: hisH [H]

Synonym: Franean1_1919

Alternate gene names: 158313754

Gene position: 2300392-2301006 (Clockwise)

Preceding gene: 158313753

Following gene: 158313758

Centisome position: 25.61

GC content: 73.66

Gene sequence:

>615_bases
TTGAGCGGCCCGCGGCCGGACGTCGTCGTCCTCGACTACGGCTCGGGGAACCTGCGTTCGGCCGAGCGGGCGCTCGCCCG
GGTCGGTGCCGAGGTGACGGTGACGGCGGATCTCGACGCCGCCCGGGCCGCCGACGGGCTCGTCGTCCCCGGGGTCGGCG
CCTTCGCGGCGTGCATGGCGGGTGTGGATGCGGTCGGCGGCGGCGAGGTCGTGCGTGAGCGGCTGGCTGCCGGCCGACCG
GTCCTCGGGATCTGCGTGGGCATGCAGATCCTCTACCAGCTCGGCGACGAGCATGGTGTACGGACGGAGGGCCTCGGCGT
GCTCGAGGGGAGTGTGCGCCGGCTGACGGCGCCGGTCCTGCCGCACATGGGCTGGAACGTCGTCACGCCCCCACCGGAGT
CGGTCCTGTTCGAGGGCGTCGACGCGGAGACGCGTTTCTACTTCGTGCACTCTTACGCGGCGGCGGCGCGGGCGGGTGAC
ACCGTCGCCGAACACGGCGAGGCGTTCGCCGCCGCGGTCGAGCGTGGACCGCTCGCGGCCACCCAGTTCCATCCGGAGAA
GTCCGGCGACGCCGGTGCCCACGTCCTGCGGAACTGGCTGCGCACCCTGAGCTGA

Upstream 100 bases:

>100_bases
ACGTCGTCGAGGCGCAGTTCAAGTCGGTGGCCCGGGCCATGCGCGACGCTGTGGCGCTCGACGCGCGGGTCGCCGGCGTG
CCGTCCACCAAGGGCGTGCT

Downstream 100 bases:

>100_bases
CCACCTGCAGCGGCCAGCCGCCGAGCCGCCGCAGCCGCCGCCGCCACAGGCGCAGGCGTCGGCTCCGGCCCTGTTGATCA
GGGGGCGTCGGGGGCGAGTC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MSGPRPDVVVLDYGSGNLRSAERALARVGAEVTVTADLDAARAADGLVVPGVGAFAACMAGVDAVGGGEVVRERLAAGRP
VLGICVGMQILYQLGDEHGVRTEGLGVLEGSVRRLTAPVLPHMGWNVVTPPPESVLFEGVDAETRFYFVHSYAAAARAGD
TVAEHGEAFAAAVERGPLAATQFHPEKSGDAGAHVLRNWLRTLS

Sequences:

>Translated_204_residues
MSGPRPDVVVLDYGSGNLRSAERALARVGAEVTVTADLDAARAADGLVVPGVGAFAACMAGVDAVGGGEVVRERLAAGRP
VLGICVGMQILYQLGDEHGVRTEGLGVLEGSVRRLTAPVLPHMGWNVVTPPPESVLFEGVDAETRFYFVHSYAAAARAGD
TVAEHGEAFAAAVERGPLAATQFHPEKSGDAGAHVLRNWLRTLS
>Mature_203_residues
SGPRPDVVVLDYGSGNLRSAERALARVGAEVTVTADLDAARAADGLVVPGVGAFAACMAGVDAVGGGEVVRERLAAGRPV
LGICVGMQILYQLGDEHGVRTEGLGVLEGSVRRLTAPVLPHMGWNVVTPPPESVLFEGVDAETRFYFVHSYAAAARAGDT
VAEHGEAFAAAVERGPLAATQFHPEKSGDAGAHVLRNWLRTLS

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=199, Percent_Identity=43.7185929648241, Blast_Score=139, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6319725, Length=216, Percent_Identity=34.7222222222222, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21165; Mature: 21034

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPRPDVVVLDYGSGNLRSAERALARVGAEVTVTADLDAARAADGLVVPGVGAFAACMA
CCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCHHHCCCCEEECCHHHHHHHHH
GVDAVGGGEVVRERLAAGRPVLGICVGMQILYQLGDEHGVRTEGLGVLEGSVRRLTAPVL
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
PHMGWNVVTPPPESVLFEGVDAETRFYFVHSYAAAARAGDTVAEHGEAFAAAVERGPLAA
CCCCCCCCCCCCHHHEECCCCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHCCCCCE
TQFHPEKSGDAGAHVLRNWLRTLS
EECCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGPRPDVVVLDYGSGNLRSAERALARVGAEVTVTADLDAARAADGLVVPGVGAFAACMA
CCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCHHHCCCCEEECCHHHHHHHHH
GVDAVGGGEVVRERLAAGRPVLGICVGMQILYQLGDEHGVRTEGLGVLEGSVRRLTAPVL
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
PHMGWNVVTPPPESVLFEGVDAETRFYFVHSYAAAARAGDTVAEHGEAFAAAVERGPLAA
CCCCCCCCCCCCHHHEECCCCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHCCCCCE
TQFHPEKSGDAGAHVLRNWLRTLS
EECCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA