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The map label for this gene is hisA

Identifier: 158313758

GI number: 158313758

Start: 2304007

End: 2304744

Strand: Direct

Name: hisA

Synonym: Franean1_1923

Alternate gene names: 158313758

Gene position: 2304007-2304744 (Clockwise)

Preceding gene: 158313754

Following gene: 229595062

Centisome position: 25.65

GC content: 75.75

Gene sequence:

>738_bases
GTGACGTTGACGCTGCTACCCGCGGTGGATGTGGCCGACGGCCTGGCCGTCCGCCTGGTCCAGGGCGAGGCCGGAACCGA
GACCTCCTACGGCGACCCGCGCGAGGCGGCGCTGGCCTGGCAGCGCGACGGCGCCGAGTGGATCCATCTGGTCGACCTCG
ACGCGGCCTTCGGCCGCGGGTCCAACCGTGACCTGATCGCCGAGGTCGTCCGCTCGGTCGACGTGGCCGTGGAGCTCTCC
GGCGGCATTCGGGACGACGACTCCCTCGACGCCGCGCTGGCCACCGGCGCGGCCCGGGTCAACATCGGCACGGCGGCGCT
CGAGGACCCGGAGTGGGTGCGCAAGGCGATCGACCGGGTCGGCGACCGGATCGCCGTGGGCCTGGACGTCCGTGGCACCA
CCCTCGCCGCGCGGGGCTGGACGCGCGAGGGCGGCGAGCTCTACGAGGTGCTCGCACGGCTGGACGCCGACGGCTGCGCC
CGCTACGTGCTCACCGACGTCCGCCGGGACGGGACCCTCACCGGCCCGAACGTCGAGCTGCTGCGGGCCGTGACGGCCGC
GACGACCCGGCCGGTGGTCGCCAGCGGCGGCGTCTCCTCCCTGGAGGACCTGCGCGTGATCGCGACGGTGCCCGGTGTCG
AGGGCGCCATTGTCGGCAAGGCGCTCTACGCCGGCGCGTTCACCCTGCCCGAGGCGCTCGCGGTCGCGGCGGAGGTGCCC
GCGGCGGGCGCACCGTGA

Upstream 100 bases:

>100_bases
ACGCTAGAGCGATCCGTGGTCGGAGGATCCCAGGTTGCGGCAACTCGTGTGGTCCGGTGGTGCGCCTCCTCGAGGGCCGG
GTGACCCGTAGGGTGGGGAT

Downstream 100 bases:

>100_bases
CCGTCGCCGTCCGGGTGATCCCCTGCCTTGACGTCGACGGCGGGCGGGTCGTCAAGGGTGTGAACTTCACCGACCTGCGG
GACGCGGGCGACCCGGTCGA

Product: phosphoribosyl isomerase A

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTLTLLPAVDVADGLAVRLVQGEAGTETSYGDPREAALAWQRDGAEWIHLVDLDAAFGRGSNRDLIAEVVRSVDVAVELS
GGIRDDDSLDAALATGAARVNIGTAALEDPEWVRKAIDRVGDRIAVGLDVRGTTLAARGWTREGGELYEVLARLDADGCA
RYVLTDVRRDGTLTGPNVELLRAVTAATTRPVVASGGVSSLEDLRVIATVPGVEGAIVGKALYAGAFTLPEALAVAAEVP
AAGAP

Sequences:

>Translated_245_residues
MTLTLLPAVDVADGLAVRLVQGEAGTETSYGDPREAALAWQRDGAEWIHLVDLDAAFGRGSNRDLIAEVVRSVDVAVELS
GGIRDDDSLDAALATGAARVNIGTAALEDPEWVRKAIDRVGDRIAVGLDVRGTTLAARGWTREGGELYEVLARLDADGCA
RYVLTDVRRDGTLTGPNVELLRAVTAATTRPVVASGGVSSLEDLRVIATVPGVEGAIVGKALYAGAFTLPEALAVAAEVP
AAGAP
>Mature_244_residues
TLTLLPAVDVADGLAVRLVQGEAGTETSYGDPREAALAWQRDGAEWIHLVDLDAAFGRGSNRDLIAEVVRSVDVAVELSG
GIRDDDSLDAALATGAARVNIGTAALEDPEWVRKAIDRVGDRIAVGLDVRGTTLAARGWTREGGELYEVLARLDADGCAR
YVLTDVRRDGTLTGPNVELLRAVTAATTRPVVASGGVSSLEDLRVIATVPGVEGAIVGKALYAGAFTLPEALAVAAEVPA
AGAP

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=243, Percent_Identity=36.6255144032922, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=24.21875, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_FRASN (A8LGR0)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001506266.1
- ProteinModelPortal:   A8LGR0
- GeneID:   5670324
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_1923
- HOGENOM:   HBG541613
- OMA:   CARYVVT
- ProtClustDB:   PRK14024
- BioCyc:   FSP1855:FRANEAN1_1923-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR010188
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR01919

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 25350; Mature: 25219

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: ACT_SITE 10-10 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTLLPAVDVADGLAVRLVQGEAGTETSYGDPREAALAWQRDGAEWIHLVDLDAAFGRG
CEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHEEECCCCCEEEEEEEHHHHCCC
SNRDLIAEVVRSVDVAVELSGGIRDDDSLDAALATGAARVNIGTAALEDPEWVRKAIDRV
CCHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHC
GDRIAVGLDVRGTTLAARGWTREGGELYEVLARLDADGCARYVLTDVRRDGTLTGPNVEL
CCEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHH
LRAVTAATTRPVVASGGVSSLEDLRVIATVPGVEGAIVGKALYAGAFTLPEALAVAAEVP
HHHHHHHCCCCEEECCCCCCHHHEEEEEECCCCCCHHHHHHHHHCHHHHHHHHHHHHHCC
AAGAP
CCCCC
>Mature Secondary Structure 
TLTLLPAVDVADGLAVRLVQGEAGTETSYGDPREAALAWQRDGAEWIHLVDLDAAFGRG
EEEEECCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHEEECCCCCEEEEEEEHHHHCCC
SNRDLIAEVVRSVDVAVELSGGIRDDDSLDAALATGAARVNIGTAALEDPEWVRKAIDRV
CCHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHC
GDRIAVGLDVRGTTLAARGWTREGGELYEVLARLDADGCARYVLTDVRRDGTLTGPNVEL
CCEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHH
LRAVTAATTRPVVASGGVSSLEDLRVIATVPGVEGAIVGKALYAGAFTLPEALAVAAEVP
HHHHHHHCCCCEEECCCCCCHHHEEEEEECCCCCCHHHHHHHHHCHHHHHHHHHHHHHCC
AAGAP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA