Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 157964067

GI number: 157964067

Start: 5170510

End: 5171871

Strand: Reverse

Name: trmE

Synonym: Spea_4256

Alternate gene names: 157964067

Gene position: 5171871-5170510 (Counterclockwise)

Preceding gene: 157964068

Following gene: 157964065

Centisome position: 99.95

GC content: 47.36

Gene sequence:

>1362_bases
ATGACTACAGATACTATCGTGGCACAAGCGACCGCTCCTGGGCGTGGTGGCGTGGGTATTATTCGCGTATCTGGCGACTT
AGCGACTAATGTTGCTATAGCCGTTTTAGGACATATCCCTAAAACCCGTTACGCCGATTACTGTGATTTTAAAGATGAGG
CTGGTGAAGTCATCGACCAAGGTATCGCCTTGTTCTTTAAAGGCCCAAACTCCTTTACCGGTGAAGACGTTCTGGAACTG
CAAGGCCACGGCGGTCAAATAGTTCTCGATATGCTGATTAAGCGCGTCATGGAAGTCGATGGGCTGCGTATCGCAAAACC
GGGTGAATTCAGCGAACAGGCTTTCATGAATGATAAGCTTGACCTGACTCAAGCCGAAGCGATTGCCGACCTAATTGATG
CCACAAGTGAACAAGCTGCCAAAAGCGCTCTCAACTCACTTCAGGGCGAATTCTCTACTCAGATACATGATCTGGTCGAA
AAGGTCACAAACTTGCGCTTATACGTCGAGGCGGCAATTGATTTCCCTGATGAAGAGGTCGATTTCCTGTCCGACGGTAA
AATAGCCGCCTCCTTGAATGGGATTGTTGGCAAACTCGATGGGGTTCAAGCTAGCGCTAAACAGGGCTCTATTATTCGTG
AAGGCATGAAAGTAGTGATTGCAGGTCGTCCAAATGCGGGTAAATCAAGCCTACTTAACGCCTTGGCTGGCAAAGAGTCA
GCTATTGTGACCGAAATAGCTGGCACCACTCGCGATGTATTACGTGAACATATCCATTTAGATGGTATGCCATTACACAT
TATTGATACCGCAGGTTTACGTGACACCGTCGATACCGTAGAGAAAATTGGTATCGAGCGCGCTTGGGATGAGATAAAAA
CAGCCGATCGCGTACTGTTTATGGTCGATGGCACCACAACTGCAGCCATCGATCCTCATGAAATCTGGCCAGACTTTATC
GATCGTCTCCCTAGTAACTTAGGCGTTACCGTGGTACGTAACAAAGCCGATCTAACCGGTGAAGATCTTTCCGTGACTCA
AGAAGCGGGACATAGCGTGTATCGTATCTCAGCCAAAACAGGCCTAGGTGTCGAAGACCTGAAGCAACATCTAAAATCAT
TAATGGGTTACCAGAGTAATCTAGAGGGCGGCTTTATTGCTCGACGTCGTCACTTAGAGGCACTAGATCTAGCTAGCAGT
CACTTGATGCTAGGTAAAGAGCAATTGGAAGTGTATCAAGCTGGTGAACTGCTCGCCGAAGAACTACGCATGTGTCAGAT
GGCACTATCTGAGATCACAGGTAAGTTCACTTCAGATGATCTTCTCGGCAAGATCTTTAGTTCATTCTGTATTGGCAAGT
AA

Upstream 100 bases:

>100_bases
ATAAAAAGCGGTCAATACTGAGTATTGACCGCTTTTTTGCTTTCTATGTAGAGACAATATTTTTAACTTCACCCGAAAAA
TCAAATTAGGAAACACAGTA

Downstream 100 bases:

>100_bases
AATAGCTTAAATTAAGAACAATTTAACAAAATAGAAAGCCACAGCAGACTTAACTACTGTGGCTTTTTGCTTTTCTAAAT
AGCTAACGTATTGCAGATGG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTTDTIVAQATAPGRGGVGIIRVSGDLATNVAIAVLGHIPKTRYADYCDFKDEAGEVIDQGIALFFKGPNSFTGEDVLEL
QGHGGQIVLDMLIKRVMEVDGLRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDATSEQAAKSALNSLQGEFSTQIHDLVE
KVTNLRLYVEAAIDFPDEEVDFLSDGKIAASLNGIVGKLDGVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTVDTVEKIGIERAWDEIKTADRVLFMVDGTTTAAIDPHEIWPDFI
DRLPSNLGVTVVRNKADLTGEDLSVTQEAGHSVYRISAKTGLGVEDLKQHLKSLMGYQSNLEGGFIARRRHLEALDLASS
HLMLGKEQLEVYQAGELLAEELRMCQMALSEITGKFTSDDLLGKIFSSFCIGK

Sequences:

>Translated_453_residues
MTTDTIVAQATAPGRGGVGIIRVSGDLATNVAIAVLGHIPKTRYADYCDFKDEAGEVIDQGIALFFKGPNSFTGEDVLEL
QGHGGQIVLDMLIKRVMEVDGLRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDATSEQAAKSALNSLQGEFSTQIHDLVE
KVTNLRLYVEAAIDFPDEEVDFLSDGKIAASLNGIVGKLDGVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTVDTVEKIGIERAWDEIKTADRVLFMVDGTTTAAIDPHEIWPDFI
DRLPSNLGVTVVRNKADLTGEDLSVTQEAGHSVYRISAKTGLGVEDLKQHLKSLMGYQSNLEGGFIARRRHLEALDLASS
HLMLGKEQLEVYQAGELLAEELRMCQMALSEITGKFTSDDLLGKIFSSFCIGK
>Mature_452_residues
TTDTIVAQATAPGRGGVGIIRVSGDLATNVAIAVLGHIPKTRYADYCDFKDEAGEVIDQGIALFFKGPNSFTGEDVLELQ
GHGGQIVLDMLIKRVMEVDGLRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDATSEQAAKSALNSLQGEFSTQIHDLVEK
VTNLRLYVEAAIDFPDEEVDFLSDGKIAASLNGIVGKLDGVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKESA
IVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTVDTVEKIGIERAWDEIKTADRVLFMVDGTTTAAIDPHEIWPDFID
RLPSNLGVTVVRNKADLTGEDLSVTQEAGHSVYRISAKTGLGVEDLKQHLKSLMGYQSNLEGGFIARRRHLEALDLASSH
LMLGKEQLEVYQAGELLAEELRMCQMALSEITGKFTSDDLLGKIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=473, Percent_Identity=34.0380549682875, Blast_Score=221, Evalue=2e-57,
Organism=Homo sapiens, GI193082993, Length=463, Percent_Identity=33.9092872570194, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI193082988, Length=505, Percent_Identity=31.8811881188119, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=72.2838137472284, Blast_Score=678, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=92, Percent_Identity=38.0434782608696, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=469, Percent_Identity=29.4243070362473, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6323665, Length=497, Percent_Identity=30.3822937625755, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI45550813, Length=473, Percent_Identity=31.9238900634249, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_SHEPA (A8HAH9)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001504101.1
- ProteinModelPortal:   A8HAH9
- SMR:   A8HAH9
- STRING:   A8HAH9
- GeneID:   5664640
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_4256
- NMPDR:   fig|398579.3.peg.3696
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   SPEA398579:SPEA_4256-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48887; Mature: 48756

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: BINDING 22-22 BINDING 79-79 BINDING 119-119 BINDING 453-453

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDTIVAQATAPGRGGVGIIRVSGDLATNVAIAVLGHIPKTRYADYCDFKDEAGEVIDQ
CCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHC
GIALFFKGPNSFTGEDVLELQGHGGQIVLDMLIKRVMEVDGLRIAKPGEFSEQAFMNDKL
CEEEEEECCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHCCCEECCCCCCCHHHHCCCCC
DLTQAEAIADLIDATSEQAAKSALNSLQGEFSTQIHDLVEKVTNLRLYVEAAIDFPDEEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCC
DFLSDGKIAASLNGIVGKLDGVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
CCCCCCCEEEEHHHHHHCCCCCCCCCCCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCCC
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTVDTVEKIGIERAWDEIKTADRVLF
HHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
MVDGTTTAAIDPHEIWPDFIDRLPSNLGVTVVRNKADLTGEDLSVTQEAGHSVYRISAKT
EECCCEEECCCHHHHCHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHCCCCEEEEECCC
GLGVEDLKQHLKSLMGYQSNLEGGFIARRRHLEALDLASSHLMLGKEQLEVYQAGELLAE
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELRMCQMALSEITGKFTSDDLLGKIFSSFCIGK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTDTIVAQATAPGRGGVGIIRVSGDLATNVAIAVLGHIPKTRYADYCDFKDEAGEVIDQ
CCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHC
GIALFFKGPNSFTGEDVLELQGHGGQIVLDMLIKRVMEVDGLRIAKPGEFSEQAFMNDKL
CEEEEEECCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHCCCEECCCCCCCHHHHCCCCC
DLTQAEAIADLIDATSEQAAKSALNSLQGEFSTQIHDLVEKVTNLRLYVEAAIDFPDEEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCC
DFLSDGKIAASLNGIVGKLDGVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
CCCCCCCEEEEHHHHHHCCCCCCCCCCCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCCC
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTVDTVEKIGIERAWDEIKTADRVLF
HHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
MVDGTTTAAIDPHEIWPDFIDRLPSNLGVTVVRNKADLTGEDLSVTQEAGHSVYRISAKT
EECCCEEECCCHHHHCHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHCCCCEEEEECCC
GLGVEDLKQHLKSLMGYQSNLEGGFIARRRHLEALDLASSHLMLGKEQLEVYQAGELLAE
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELRMCQMALSEITGKFTSDDLLGKIFSSFCIGK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA