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The map label for this gene is fadD [C]

Identifier: 157963693

GI number: 157963693

Start: 4727800

End: 4729455

Strand: Direct

Name: fadD [C]

Synonym: Spea_3882

Alternate gene names: 157963693

Gene position: 4727800-4729455 (Clockwise)

Preceding gene: 157963692

Following gene: 157963694

Centisome position: 91.37

GC content: 46.86

Gene sequence:

>1656_bases
ATGGAATCTTCTATGAAAACCCCAATTGAAATGCTCGAACATTGGGTTGAAAAACAAGGTGATCAAGTTTACCTAAAGCA
ACCTATTGACGGCCAATATAAGACTTTTACTTGGCGTGATGTACAAACCAAGATGCAGCAGATTGCAGGCGCATTGAGAC
ACCTTGGCCTCAATCCTGGTGACAAAGTTGCCGTGCTTTCTAAAAACTGTGCCGAATGGTTTATTACTGATTTAGCCCTG
ATGCACGGCGGCTATATTAGTGTCCCCATCTACCCTACCGCTAACGCAGACACTATTCGCTACACCCTAGAGCACAGTGA
AGCTAAGGCAATTTTTATCGGTAAGCTCGATTATTGGGCCGATCAAGAAGCGGGTGTTGGCGGCGATATTCTGCGTATTG
CTATGCCGTACGACACCATGCCATCTCAGTATCATTGGGATAAGATGCTCAATATGGGCCAGCCTTTAATTGATGCGCCG
CTGCCAACACCTGAGCAAGTGATGACCATCATCTATACATCAGGCTCGACTGGTAAGCCTAAAGGTGCGATTCAAAATTT
TGCCAGCTATGCCTGGACTTGCACAGCGGTAGTACGCGACCTTAAAACAGACGTCGAAGACAGATTACTGTCTTACTTGC
CACTGGCACATATTACCGAGCGTGTCGCGATTCAAGGTTCTTCTTTCTACTCAGGTAGTAGTGTTGCCTTCGTCGAGAGC
CTAGATAGCTTCGTTGCCGACGTGCAGCGTGCAAGACCCACAGTATTCTTCTCAGTGCCACGCCTGTGGAGCCTATTCCA
GAAAAATATTATCGATAAGATTGGCTATAGCAAGCTTAACTTTTTGCTTAAAGTGCCGATCATTAGCAGCATAGTAAAAC
GTAAAATTCATCAAGGTTTAGGTCTAGAGCACTGCCACCTGCTCGGCTCAGGTTCTGCGCCGATCCCGCCATCACTGGTT
GAGTGGTATCACAAGATTGGTCTCGATATCAGTGAGGCTTGGGGCATGACCGAAAACTGCGCCTACTCAATTATTAACTA
CCCATTTGATGCCCGTAAAATTGGCTCCGTTGGCCGTGCGGTCGAAGGCTGTGAAGCCAAGTGTTCGGATGTTGGTGAGC
TGCTAGTTAAGAGCCCAGGCCTAATGCAAGGTTATTACAAGCAAGAGGAAGAGACAGCTAAGAGCTTCGATGACGATGGC
TTCTTCCACACTGGTGATCTCTGCTCTATCGATGATGAGGGCAACGTCAGCATTACTGGCCGTGTTAAGGATAACTTCAA
GACTTCGAAAGGTAAGTATGTTGCTCCAGTACCGATTGAGCGCAAATTGGCACAAGATCCTCACATAGAGATGATCTGTG
TTATCGGCTCAGGCCTGCCACACCCAATTGCCTTAGTGCAATTGTCTGAAGGCGCTACACTGCAACCTAGGGAAGAAGTA
AGGCTATCGCTGAAAAACACCATAGATGCGGTAAACCCACACCTAGAGTCACATGAAACAGTCGATGCTATCGTTATTGT
GAATGAAGCTTGGGATGTTGAAAATGACGTACTCACTCCAACCCTTAAGATTAAACGCCATGTGCTTGAGAAGAAGTTTA
GTGAGAAGGTCGACGGCATTCGTGGCGGAAAAGTTTGCTGGGAAGATGAAATCTAA

Upstream 100 bases:

>100_bases
CGCCATAATCAAGATTGCAACAGCTTGTTAAACGAACGATTAAAACAAAAGTACAGGTATAAGCTGAATTAGAACCGACA
ACAATCAATATGGAAATGCG

Downstream 100 bases:

>100_bases
GCTAGAGTTATTTTAATCGCCATGCATACGGCACTCTTCAGGGGTGCCGTTTTTATTTGCTGGATAAAAAACCATCAATT
AACTAAATTTAATGACTAAG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase; LACS [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MESSMKTPIEMLEHWVEKQGDQVYLKQPIDGQYKTFTWRDVQTKMQQIAGALRHLGLNPGDKVAVLSKNCAEWFITDLAL
MHGGYISVPIYPTANADTIRYTLEHSEAKAIFIGKLDYWADQEAGVGGDILRIAMPYDTMPSQYHWDKMLNMGQPLIDAP
LPTPEQVMTIIYTSGSTGKPKGAIQNFASYAWTCTAVVRDLKTDVEDRLLSYLPLAHITERVAIQGSSFYSGSSVAFVES
LDSFVADVQRARPTVFFSVPRLWSLFQKNIIDKIGYSKLNFLLKVPIISSIVKRKIHQGLGLEHCHLLGSGSAPIPPSLV
EWYHKIGLDISEAWGMTENCAYSIINYPFDARKIGSVGRAVEGCEAKCSDVGELLVKSPGLMQGYYKQEEETAKSFDDDG
FFHTGDLCSIDDEGNVSITGRVKDNFKTSKGKYVAPVPIERKLAQDPHIEMICVIGSGLPHPIALVQLSEGATLQPREEV
RLSLKNTIDAVNPHLESHETVDAIVIVNEAWDVENDVLTPTLKIKRHVLEKKFSEKVDGIRGGKVCWEDEI

Sequences:

>Translated_551_residues
MESSMKTPIEMLEHWVEKQGDQVYLKQPIDGQYKTFTWRDVQTKMQQIAGALRHLGLNPGDKVAVLSKNCAEWFITDLAL
MHGGYISVPIYPTANADTIRYTLEHSEAKAIFIGKLDYWADQEAGVGGDILRIAMPYDTMPSQYHWDKMLNMGQPLIDAP
LPTPEQVMTIIYTSGSTGKPKGAIQNFASYAWTCTAVVRDLKTDVEDRLLSYLPLAHITERVAIQGSSFYSGSSVAFVES
LDSFVADVQRARPTVFFSVPRLWSLFQKNIIDKIGYSKLNFLLKVPIISSIVKRKIHQGLGLEHCHLLGSGSAPIPPSLV
EWYHKIGLDISEAWGMTENCAYSIINYPFDARKIGSVGRAVEGCEAKCSDVGELLVKSPGLMQGYYKQEEETAKSFDDDG
FFHTGDLCSIDDEGNVSITGRVKDNFKTSKGKYVAPVPIERKLAQDPHIEMICVIGSGLPHPIALVQLSEGATLQPREEV
RLSLKNTIDAVNPHLESHETVDAIVIVNEAWDVENDVLTPTLKIKRHVLEKKFSEKVDGIRGGKVCWEDEI
>Mature_551_residues
MESSMKTPIEMLEHWVEKQGDQVYLKQPIDGQYKTFTWRDVQTKMQQIAGALRHLGLNPGDKVAVLSKNCAEWFITDLAL
MHGGYISVPIYPTANADTIRYTLEHSEAKAIFIGKLDYWADQEAGVGGDILRIAMPYDTMPSQYHWDKMLNMGQPLIDAP
LPTPEQVMTIIYTSGSTGKPKGAIQNFASYAWTCTAVVRDLKTDVEDRLLSYLPLAHITERVAIQGSSFYSGSSVAFVES
LDSFVADVQRARPTVFFSVPRLWSLFQKNIIDKIGYSKLNFLLKVPIISSIVKRKIHQGLGLEHCHLLGSGSAPIPPSLV
EWYHKIGLDISEAWGMTENCAYSIINYPFDARKIGSVGRAVEGCEAKCSDVGELLVKSPGLMQGYYKQEEETAKSFDDDG
FFHTGDLCSIDDEGNVSITGRVKDNFKTSKGKYVAPVPIERKLAQDPHIEMICVIGSGLPHPIALVQLSEGATLQPREEV
RLSLKNTIDAVNPHLESHETVDAIVIVNEAWDVENDVLTPTLKIKRHVLEKKFSEKVDGIRGGKVCWEDEI

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG1022

COG function: function code I; Long-chain acyl-CoA synthetases (AMP-forming)

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI83745141, Length=617, Percent_Identity=24.3111831442464, Blast_Score=195, Evalue=1e-49,
Organism=Homo sapiens, GI42794760, Length=611, Percent_Identity=26.0229132569558, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI42794758, Length=611, Percent_Identity=26.0229132569558, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI42794756, Length=611, Percent_Identity=26.0229132569558, Blast_Score=174, Evalue=3e-43,
Organism=Homo sapiens, GI40807491, Length=595, Percent_Identity=26.3865546218487, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI57165412, Length=590, Percent_Identity=26.4406779661017, Blast_Score=158, Evalue=2e-38,
Organism=Homo sapiens, GI57165410, Length=587, Percent_Identity=25.7240204429302, Blast_Score=153, Evalue=5e-37,
Organism=Homo sapiens, GI27477105, Length=496, Percent_Identity=24.5967741935484, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI4758332, Length=622, Percent_Identity=24.2765273311897, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI12669909, Length=622, Percent_Identity=24.2765273311897, Blast_Score=147, Evalue=4e-35,
Organism=Homo sapiens, GI42794754, Length=625, Percent_Identity=23.84, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI42794752, Length=625, Percent_Identity=23.84, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI156151445, Length=474, Percent_Identity=22.5738396624473, Blast_Score=97, Evalue=5e-20,
Organism=Escherichia coli, GI1788107, Length=488, Percent_Identity=23.5655737704918, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI221142682, Length=491, Percent_Identity=26.0692464358452, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI145693145, Length=459, Percent_Identity=22.2222222222222, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786810, Length=455, Percent_Identity=26.1538461538462, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789201, Length=290, Percent_Identity=27.5862068965517, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17510401, Length=577, Percent_Identity=27.5563258232236, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17556552, Length=590, Percent_Identity=26.271186440678, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17541856, Length=564, Percent_Identity=27.3049645390071, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25147511, Length=613, Percent_Identity=23.6541598694943, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI193204819, Length=591, Percent_Identity=25.3807106598985, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17553312, Length=634, Percent_Identity=25.5520504731861, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17564090, Length=560, Percent_Identity=25.5357142857143, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17558820, Length=297, Percent_Identity=30.976430976431, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=538, Percent_Identity=22.6765799256506, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI32563687, Length=323, Percent_Identity=29.4117647058824, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71994703, Length=497, Percent_Identity=23.3400402414487, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71994694, Length=497, Percent_Identity=23.3400402414487, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71994690, Length=495, Percent_Identity=23.4343434343434, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17557194, Length=456, Percent_Identity=25.6578947368421, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538037, Length=459, Percent_Identity=26.1437908496732, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI133901848, Length=218, Percent_Identity=29.3577981651376, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=331, Percent_Identity=25.0755287009063, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320852, Length=575, Percent_Identity=27.304347826087, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6323903, Length=592, Percent_Identity=25.8445945945946, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324893, Length=591, Percent_Identity=24.8730964467005, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6322182, Length=592, Percent_Identity=24.6621621621622, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI17933690, Length=617, Percent_Identity=25.4457050243112, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI19921316, Length=619, Percent_Identity=22.2940226171244, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI281366413, Length=577, Percent_Identity=24.4367417677643, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24666501, Length=580, Percent_Identity=24.4827586206897, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24666497, Length=580, Percent_Identity=24.4827586206897, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI62471679, Length=600, Percent_Identity=23, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI62471683, Length=600, Percent_Identity=23, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI62471685, Length=600, Percent_Identity=23, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24586636, Length=600, Percent_Identity=23, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI62471689, Length=600, Percent_Identity=23, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI62471681, Length=600, Percent_Identity=23, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI62471687, Length=600, Percent_Identity=23, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24586634, Length=600, Percent_Identity=23, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI22026970, Length=600, Percent_Identity=23, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21358303, Length=455, Percent_Identity=24.3956043956044, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18859661, Length=549, Percent_Identity=24.5901639344262, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21356441, Length=542, Percent_Identity=23.4317343173432, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21355181, Length=494, Percent_Identity=23.8866396761134, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281365686, Length=134, Percent_Identity=33.5820895522388, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 61363; Mature: 61363

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESSMKTPIEMLEHWVEKQGDQVYLKQPIDGQYKTFTWRDVQTKMQQIAGALRHLGLNPG
CCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCC
DKVAVLSKNCAEWFITDLALMHGGYISVPIYPTANADTIRYTLEHSEAKAIFIGKLDYWA
CCEEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCC
DQEAGVGGDILRIAMPYDTMPSQYHWDKMLNMGQPLIDAPLPTPEQVMTIIYTSGSTGKP
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHEEEEEEECCCCCCC
KGAIQNFASYAWTCTAVVRDLKTDVEDRLLSYLPLAHITERVAIQGSSFYSGSSVAFVES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEECCCCCCCCCCHHHHHH
LDSFVADVQRARPTVFFSVPRLWSLFQKNIIDKIGYSKLNFLLKVPIISSIVKRKIHQGL
HHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCHHHHHHHEECHHHHHHHHHHHHHCC
GLEHCHLLGSGSAPIPPSLVEWYHKIGLDISEAWGMTENCAYSIINYPFDARKIGSVGRA
CCCCEEEECCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHH
VEGCEAKCSDVGELLVKSPGLMQGYYKQEEETAKSFDDDGFFHTGDLCSIDDEGNVSITG
HHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEECCCEEECCCCCCEEEEE
RVKDNFKTSKGKYVAPVPIERKLAQDPHIEMICVIGSGLPHPIALVQLSEGATLQPREEV
EECCCCCCCCCCEECCCCHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCHHHH
RLSLKNTIDAVNPHLESHETVDAIVIVNEAWDVENDVLTPTLKIKRHVLEKKFSEKVDGI
HHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
RGGKVCWEDEI
CCCCEEECCCC
>Mature Secondary Structure
MESSMKTPIEMLEHWVEKQGDQVYLKQPIDGQYKTFTWRDVQTKMQQIAGALRHLGLNPG
CCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCC
DKVAVLSKNCAEWFITDLALMHGGYISVPIYPTANADTIRYTLEHSEAKAIFIGKLDYWA
CCEEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCC
DQEAGVGGDILRIAMPYDTMPSQYHWDKMLNMGQPLIDAPLPTPEQVMTIIYTSGSTGKP
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHEEEEEEECCCCCCC
KGAIQNFASYAWTCTAVVRDLKTDVEDRLLSYLPLAHITERVAIQGSSFYSGSSVAFVES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEECCCCCCCCCCHHHHHH
LDSFVADVQRARPTVFFSVPRLWSLFQKNIIDKIGYSKLNFLLKVPIISSIVKRKIHQGL
HHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCHHHHHHHEECHHHHHHHHHHHHHCC
GLEHCHLLGSGSAPIPPSLVEWYHKIGLDISEAWGMTENCAYSIINYPFDARKIGSVGRA
CCCCEEEECCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHH
VEGCEAKCSDVGELLVKSPGLMQGYYKQEEETAKSFDDDGFFHTGDLCSIDDEGNVSITG
HHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEECCCEEECCCCCCEEEEE
RVKDNFKTSKGKYVAPVPIERKLAQDPHIEMICVIGSGLPHPIALVQLSEGATLQPREEV
EECCCCCCCCCCEECCCCHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCHHHH
RLSLKNTIDAVNPHLESHETVDAIVIVNEAWDVENDVLTPTLKIKRHVLEKKFSEKVDGI
HHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
RGGKVCWEDEI
CCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]