Click here to switch to the map view.

The map label for this gene is trmH [H]

Identifier: 157963692

GI number: 157963692

Start: 4726801

End: 4727487

Strand: Direct

Name: trmH [H]

Synonym: Spea_3881

Alternate gene names: 157963692

Gene position: 4726801-4727487 (Clockwise)

Preceding gene: 157963691

Following gene: 157963693

Centisome position: 91.35

GC content: 50.22

Gene sequence:

>687_bases
ATGAGTCCTGAACGTTTCGCCCGCATCAACCAAATGCTTGATAACCGCCAACCCGATCTCACCGTATGCCTAGATACCGT
GCACAAGGGTAATAACATTGCCGCCGTGCTACGTACCGCTGATGCGGCGGGCATACATGAAGTCCATGCGGTTTGGTCCG
AACTAGAAATGCGCGTATCGGGCAACACGGCGTCAGGCAGCCAACAATGGGTTAAGACTCGCGTACACCAGAGTATGGCG
CAAGCTGTAGAAGCGTTTCGCGATCAAGACATGCAGATTCTAGCAACGACCTTTTCAGATACCGCTGTCGATTTCAGAGC
GATTGATTACACCAAACCTACAGTGGTTATTATGGGTAACGAGCGTGATGGCGTCAGCCCTGAAGGCATAGCTGCTGCGG
ATCAACACATCATCATTCCAATGATAGGCATGGTTCAATCACTGAACGTATCCGTTGCTGCCGCACTGATTATGTATGAA
GCTCAACGTCAACGCACTGAGGCAGGCATGTATGGCACGCGAAAATTGGATGACGCCTATTGCCAGATTAAGCTATTTGA
GGAAGGGCACCCTATTTATGCTAAGGCTTGTCGCCGCAAGAAGCTGCCTTATCCAGCCATAGATGCAGAAGGCCAAATTG
TAGCGGATGAAGACTGGTGGCAAAAAATGCGGGCTCCTTCTGAATAA

Upstream 100 bases:

>100_bases
TTATTTAACGTTAGCTATTTAACGTTTGCCACGTTAAACCTTGTTTATCGCTCCAACAGCAACGAATAACTTAACCCAAA
GTACAAAATAGAAGCCAATC

Downstream 100 bases:

>100_bases
CTCCTTAGAACTATTACTAAGAGCCATTGCAAAGACTAGGTTCTAAGAGCCAGCTTTTGGAATAAATGCAAAACCTATAA
TCAATACCAGCTTGCCGCTG

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSPERFARINQMLDNRQPDLTVCLDTVHKGNNIAAVLRTADAAGIHEVHAVWSELEMRVSGNTASGSQQWVKTRVHQSMA
QAVEAFRDQDMQILATTFSDTAVDFRAIDYTKPTVVIMGNERDGVSPEGIAAADQHIIIPMIGMVQSLNVSVAAALIMYE
AQRQRTEAGMYGTRKLDDAYCQIKLFEEGHPIYAKACRRKKLPYPAIDAEGQIVADEDWWQKMRAPSE

Sequences:

>Translated_228_residues
MSPERFARINQMLDNRQPDLTVCLDTVHKGNNIAAVLRTADAAGIHEVHAVWSELEMRVSGNTASGSQQWVKTRVHQSMA
QAVEAFRDQDMQILATTFSDTAVDFRAIDYTKPTVVIMGNERDGVSPEGIAAADQHIIIPMIGMVQSLNVSVAAALIMYE
AQRQRTEAGMYGTRKLDDAYCQIKLFEEGHPIYAKACRRKKLPYPAIDAEGQIVADEDWWQKMRAPSE
>Mature_227_residues
SPERFARINQMLDNRQPDLTVCLDTVHKGNNIAAVLRTADAAGIHEVHAVWSELEMRVSGNTASGSQQWVKTRVHQSMAQ
AVEAFRDQDMQILATTFSDTAVDFRAIDYTKPTVVIMGNERDGVSPEGIAAADQHIIIPMIGMVQSLNVSVAAALIMYEA
QRQRTEAGMYGTRKLDDAYCQIKLFEEGHPIYAKACRRKKLPYPAIDAEGQIVADEDWWQKMRAPSE

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=227, Percent_Identity=56.8281938325991, Blast_Score=273, Evalue=7e-75,
Organism=Escherichia coli, GI1790623, Length=153, Percent_Identity=31.3725490196078, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25451; Mature: 25320

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPERFARINQMLDNRQPDLTVCLDTVHKGNNIAAVLRTADAAGIHEVHAVWSELEMRVS
CCHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHEEEC
GNTASGSQQWVKTRVHQSMAQAVEAFRDQDMQILATTFSDTAVDFRAIDYTKPTVVIMGN
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCEEEEEEC
ERDGVSPEGIAAADQHIIIPMIGMVQSLNVSVAAALIMYEAQRQRTEAGMYGTRKLDDAY
CCCCCCCCCCCCCCCEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
CQIKLFEEGHPIYAKACRRKKLPYPAIDAEGQIVADEDWWQKMRAPSE
EEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHCCCCC
>Mature Secondary Structure 
SPERFARINQMLDNRQPDLTVCLDTVHKGNNIAAVLRTADAAGIHEVHAVWSELEMRVS
CHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHEEEC
GNTASGSQQWVKTRVHQSMAQAVEAFRDQDMQILATTFSDTAVDFRAIDYTKPTVVIMGN
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCEEEEEEC
ERDGVSPEGIAAADQHIIIPMIGMVQSLNVSVAAALIMYEAQRQRTEAGMYGTRKLDDAY
CCCCCCCCCCCCCCCEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
CQIKLFEEGHPIYAKACRRKKLPYPAIDAEGQIVADEDWWQKMRAPSE
EEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]